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- PDB-8osi: Genetically encoded green ratiometric calcium indicator FNCaMP in... -

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Basic information

Entry
Database: PDB / ID: 8osi
TitleGenetically encoded green ratiometric calcium indicator FNCaMP in calcium-bound state
ComponentsmNeonGreen,Calmodulin,Protein kinase domain-containing protein
KeywordsFLUORESCENT PROTEIN / Fluorescent indicator / Fluorescent sensor / Chromophore / mNeonGreen
Function / homology
Function and homology information


bioluminescence / protein phosphorylation / protein serine/threonine kinase activity / calcium ion binding / ATP binding
Similarity search - Function
Ca2+ insensitive EF hand / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain ...Ca2+ insensitive EF hand / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Serine/threonine-protein kinase, active site / Serine/Threonine protein kinases active-site signature. / Protein kinase domain / Serine/Threonine protein kinases, catalytic domain / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily
Similarity search - Domain/homology
Chem-CR2 / Calmodulin / mNeonGreen / Protein kinase domain-containing protein
Similarity search - Component
Biological speciesBranchiostoma lanceolatum (amphioxus)
Aspergillus niger (mold)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.42 Å
AuthorsVarfolomeeva, L.A. / Boyko, K.M. / Nikolaeva, A.Y. / Subach, O.M. / Subach, F.V.
Funding support Russian Federation, 1items
OrganizationGrant numberCountry
Russian Science Foundation21-74-20135 Russian Federation
CitationJournal: Biochem.Biophys.Res.Commun. / Year: 2023
Title: FNCaMP, ratiometric green calcium indicator based on mNeonGreen protein.
Authors: Subach, O.M. / Varfolomeeva, L. / Vlaskina, A.V. / Agapova, Y.K. / Nikolaeva, A.Y. / Piatkevich, K.D. / Patrushev, M.V. / Boyko, K.M. / Subach, F.V.
History
DepositionApr 19, 2023Deposition site: PDBE / Processing site: PDBE
Revision 1.0May 24, 2023Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: mNeonGreen,Calmodulin,Protein kinase domain-containing protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)52,7166
Polymers52,2801
Non-polymers4365
Water88349
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area350 Å2
ΔGint-50 kcal/mol
Surface area18560 Å2
Unit cell
Length a, b, c (Å)151.400, 151.400, 61.969
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number154
Space group name H-MP3221

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Components

#1: Protein mNeonGreen,Calmodulin,Protein kinase domain-containing protein


Mass: 52280.168 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Branchiostoma lanceolatum (amphioxus), (gene. exp.) Aspergillus niger (mold)
Gene: blFP-Y3, ABL_02521, ATCC64974_81790, CAN33_002760, ATCC64974_70630, CAN33_0054440
Production host: Escherichia coli (E. coli)
References: UniProt: A0A1S4NYF2, UniProt: A0A100IBH9, UniProt: A0A254U4M1
#2: Chemical ChemComp-CR2 / {(4Z)-2-(aminomethyl)-4-[(4-hydroxyphenyl)methylidene]-5-oxo-4,5-dihydro-1H-imidazol-1-yl}acetic acid / CHROMOPHORE (GLY-TYR-GLY)


Type: L-peptide linking / Mass: 275.260 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C13H13N3O4 / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical
ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Ca / Feature type: SUBJECT OF INVESTIGATION
#4: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 49 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.92 Å3/Da / Density % sol: 68.64 %
Crystal growTemperature: 288 K / Method: vapor diffusion, hanging drop / Details: 0.1 M MMT buffer, pH 8.0, 25% PEG 1500

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: MASSIF-3 / Wavelength: 0.9677 Å
DetectorType: DECTRIS EIGER X 4M / Detector: PIXEL / Date: Feb 25, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9677 Å / Relative weight: 1
ReflectionResolution: 2.42→47.95 Å / Num. obs: 31109 / % possible obs: 99.4 % / Redundancy: 16.7 % / CC1/2: 0.994 / Rmerge(I) obs: 0.101 / Rpim(I) all: 0.025 / Rrim(I) all: 0.104 / Χ2: 0.93 / Net I/σ(I): 15.8 / Num. measured all: 520158
Reflection shellResolution: 2.42→2.51 Å / % possible obs: 96.1 % / Redundancy: 15.3 % / Rmerge(I) obs: 1.437 / Num. measured all: 47835 / Num. unique obs: 3135 / CC1/2: 0.835 / Rpim(I) all: 0.36 / Rrim(I) all: 1.483 / Χ2: 0.81 / Net I/σ(I) obs: 1.7

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Processing

Software
NameVersionClassification
REFMAC5.8.0405refinement
XDSdata reduction
Aimlessdata scaling
MOLREPphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.42→47.95 Å / Cor.coef. Fo:Fc: 0.96 / Cor.coef. Fo:Fc free: 0.943 / SU B: 13.971 / SU ML: 0.148 / Cross valid method: THROUGHOUT / ESU R: 0.214 / ESU R Free: 0.189 / Stereochemistry target values: MAXIMUM LIKELIHOOD
RfactorNum. reflection% reflectionSelection details
Rfree0.23104 1539 5 %RANDOM
Rwork0.1958 ---
obs0.19756 29548 99.43 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso mean: 67.15 Å2
Baniso -1Baniso -2Baniso -3
1-0.6 Å20.3 Å20 Å2
2--0.6 Å2-0 Å2
3----1.96 Å2
Refinement stepCycle: 1 / Resolution: 2.42→47.95 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3179 0 23 49 3251
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0140.0123284
X-RAY DIFFRACTIONr_bond_other_d
X-RAY DIFFRACTIONr_angle_refined_deg2.6161.6584398
X-RAY DIFFRACTIONr_angle_other_deg
X-RAY DIFFRACTIONr_dihedral_angle_1_deg8.6225397
X-RAY DIFFRACTIONr_dihedral_angle_2_deg11.591523
X-RAY DIFFRACTIONr_dihedral_angle_3_deg20.47310572
X-RAY DIFFRACTIONr_dihedral_angle_4_deg
X-RAY DIFFRACTIONr_chiral_restr0.2140.2468
X-RAY DIFFRACTIONr_gen_planes_refined0.0150.022488
X-RAY DIFFRACTIONr_gen_planes_other
X-RAY DIFFRACTIONr_nbd_refined
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it8.4955.6721602
X-RAY DIFFRACTIONr_mcbond_other
X-RAY DIFFRACTIONr_mcangle_it10.87510.1821991
X-RAY DIFFRACTIONr_mcangle_other
X-RAY DIFFRACTIONr_scbond_it11.7946.1221682
X-RAY DIFFRACTIONr_scbond_other
X-RAY DIFFRACTIONr_scangle_it
X-RAY DIFFRACTIONr_scangle_other
X-RAY DIFFRACTIONr_long_range_B_refined15.75456.324563
X-RAY DIFFRACTIONr_long_range_B_other
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
LS refinement shellResolution: 2.424→2.486 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.503 113 -
Rwork0.488 2059 -
obs--94.97 %
Refinement TLS params.Method: refined / Origin x: 67.582 Å / Origin y: -7.928 Å / Origin z: -2.052 Å
111213212223313233
T0.1409 Å20.0157 Å2-0.005 Å2-0.0691 Å2-0.0038 Å2--0.0068 Å2
L0.0777 °2-0.0349 °20.0023 °2-0.5766 °20.0611 °2--0.2242 °2
S-0.0146 Å °-0.0042 Å °-0.0136 Å °-0.074 Å °-0.0141 Å °0.0237 Å °-0.0213 Å °0.0118 Å °0.0287 Å °
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1A12 - 421
2X-RAY DIFFRACTION1A501

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