PDB-8os6: Structure of a GFRA1/GDNF LICAM complex

Basic information

• Entry: Database: PDB / ID: 8os6
• Title: Structure of a GFRA1/GDNF LICAM complex

Components

• GDNF family receptor alphaGFRα
• Glial cell line-derived neurotrophic factor

• Keywords: CELL ADHESION / Adhesion / Synapse / Complex / signalling

Function / homology

Function:

RAF/MAP kinase cascade / : / diencephalon development / positive regulation of ureteric bud formation / postganglionic parasympathetic fiber development / positive regulation of monooxygenase activity / regulation of dopaminergic neuron differentiation / glial cell-derived neurotrophic factor receptor binding / regulation of morphogenesis of a branching structure / regulation of dopamine uptake involved in synaptic transmission / enteric nervous system development / positive regulation of branching involved in ureteric bud morphogenesis / peristalsis / sympathetic nervous system development / peripheral nervous system development / mRNA stabilization / metanephros development / neural crest cell migration / branching involved in ureteric bud morphogenesis / MAP kinase kinase kinase activity / growth factor activity / receptor tyrosine kinase binding / neuron projection development / signaling receptor activity / nervous system development / protein-containing complex assembly / negative regulation of neuron apoptotic process / receptor complex / external side of plasma membrane / protein homodimerization activity / positive regulation of transcription by RNA polymerase II / extracellular space / extracellular region

Domain/homology:

: / Glial cell line-derived neurotrophic factor receptor, alpha 1 / Glial cell line-derived neurotrophic factor / Glial cell line-derived neurotrophic factor receptor, alpha 1/2 / Glial cell line-derived neurotrophic factor receptor / GDNF receptor alpha / Glial cell line-derived neurotrophic factor family / GDNF/GAS1 / GDNF/GAS1 domain / GDNF/GAS1 domain / Transforming growth factor-beta, C-terminal / Transforming growth factor beta like domain / TGF-beta family profile. / Cystine-knot cytokine

Component:

GDNF family receptor alpha / Glial cell line-derived neurotrophic factor

• Biological species: Danio rerio (zebrafish)
• Method: X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.66 Å
• Authors: Houghton, F.M. / Adams, S.E. / Briggs, D.C. / McDonald, N.Q.

Funding support

United Kingdom, 1items

Organization	Grant number	Country
The Francis Crick Institute	CC2068	United Kingdom

• Citation: Journal: Nat Commun / Year: 2023; Title: Architecture and regulation of a GDNF-GFRα1 synaptic adhesion assembly.; Authors: F M Houghton / S E Adams / A S Ríos / L Masino / A G Purkiss / D C Briggs / F Ledda / N Q McDonald / ; Abstract: Glial-cell line derived neurotrophic factor (GDNF) bound to its co-receptor GFRα1 stimulates the RET receptor tyrosine kinase, promoting neuronal survival and neuroprotection. The GDNF-GFRα1 complex also supports synaptic cell adhesion independently of RET. Here, we describe the structure of a decameric GDNF-GFRα1 assembly determined by crystallography and electron microscopy, revealing two GFRα1 pentamers bridged by five GDNF dimers. We reconsitituted the assembly between adhering liposomes and used cryo-electron tomography to visualize how the complex fulfils its membrane adhesion function. The GFRα1:GFRα1 pentameric interface was further validated both in vitro by native PAGE and in cellulo by cell-clustering and dendritic spine assays. Finally, we provide biochemical and cell-based evidence that RET and heparan sulfate cooperate to prevent assembly of the adhesion complex by competing for the adhesion interface. Our results provide a mechanistic framework to understand GDNF-driven cell adhesion, its relationship to trophic signalling, and the central role played by GFRα1.

History

Deposition	Apr 18, 2023	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Nov 29, 2023	Provider: repository / Type: Initial release
Revision 1.1	Mar 27, 2024	Group: Database references / Category: citation Item: _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title

Assembly

Deposited unit

A: GDNF family receptor alpha

B: Glial cell line-derived neurotrophic factor

C: GDNF family receptor alpha

D: Glial cell line-derived neurotrophic factor

E: GDNF family receptor alpha

F: Glial cell line-derived neurotrophic factor

G: GDNF family receptor alpha

H: Glial cell line-derived neurotrophic factor

I: GDNF family receptor alpha

J: Glial cell line-derived neurotrophic factor

K: GDNF family receptor alpha

L: Glial cell line-derived neurotrophic factor

M: GDNF family receptor alpha

N: Glial cell line-derived neurotrophic factor

O: GDNF family receptor alpha

P: Glial cell line-derived neurotrophic factor

Q: GDNF family receptor alpha

R: Glial cell line-derived neurotrophic factor

S: GDNF family receptor alpha

T: Glial cell line-derived neurotrophic factor

hetero molecules

Summary:

• 412 kDa, 20 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	411,870	38
Polymers	405,882	20
Non-polymers	5,989	18
Water	342	19

1

Summary:

• Idetical with deposited unit
• defined by author
• Evidence: electron microscopy, Negative stain electron microscopy reveals a particle with the same dimensions, 5-fold symmetry and architecture. Assembly also validated by Native PAGE and mutation of residues in the interface

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	53420 Å2
ΔGint	-123 kcal/mol
Surface area	139130 Å2

Unit cell

Length a, b, c (Å)	114.140, 170.044, 130.791
Angle α, β, γ (deg.)	90.000, 96.170, 90.000
Int Tables number	4
Space group name H-M	P1211
Space group name Hall	P2yb
Symmetry operation	#1: x,y,z #2: -x,y+1/2,-z

Components

Protein , 2 types, 20 molecules A C E G I K M O Q S B D F H J L N P R T

#1: Protein

A C E G I K M O Q S
GDNF family receptor alpha / GFRα

Details:

Mass: 29249.277 Da / Num. of mol.: 10
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Danio rerio (zebrafish) / Gene: gfralpha1a, zgc:110129, gfra1a / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q98TT9

#2: Protein

B D F H J L N P R T
Glial cell line-derived neurotrophic factor / / zGDNF

Details:

Mass: 11338.884 Da / Num. of mol.: 10
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Danio rerio (zebrafish) / Gene: gdnf / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: Q98TU0

Sugars , 4 types, 18 molecules

#3: Polysaccharide

C - [U] J - [Y] N - [Z] P - [BA]
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose

Details:

Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source

Descriptor:

Descriptor	Type	Program
DGlcpNAcb1-4DGlcpNAcb1-ROH	Glycam Condensed Sequence	GMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1	WURCS	PDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}	LINUCS	PDB-CARE

#4: Polysaccharide

D - [V] F - [W] H - [X] 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose

Details:

Type: oligosaccharide / Mass: 570.542 Da / Num. of mol.: 3 / Source method: obtained synthetically

Descriptor:

Descriptor	Type	Program
DGlcpNAcb1-4[LFucpa1-6]DGlcpNAcb1-ROH	Glycam Condensed Sequence	GMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5]/1-1-2/a4-b1_a6-c1	WURCS	PDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}[(6+1)][a-L-Fucp]{}}	LINUCS	PDB-CARE

#5: Polysaccharide

O - [AA] alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose

Details:

Type: oligosaccharide / Mass: 367.349 Da / Num. of mol.: 1 / Source method: isolated from a natural source

Descriptor:

Descriptor	Type	Program
LFucpa1-6DGlcpNAcb1-ROH	Glycam Condensed Sequence	GMML 1.0
WURCS=2.0/2,2,1/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5]/1-2/a6-b1	WURCS	PDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(6+1)][a-L-Fucp]{}}	LINUCS	PDB-CARE

#6: Sugar

B-301 E-401 G-401 I-401 K-401 L-301 M-401 R-301 S-401 T-301
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE / N-Acetylglucosamine

Details:

Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 10 / Source method: isolated from a natural source / Formula: C₈H₁₅NO₆

Identifier:

Identifier	Type	Program
DGlcpNAcb	CONDENSED IUPAC CARBOHYDRATE SYMBOL	GMML 1.0
N-acetyl-b-D-glucopyranosamine	COMMON NAME	GMML 1.0
b-D-GlcpNAc	IUPAC CARBOHYDRATE SYMBOL	PDB-CARE 1.0
GlcNAc	SNFG CARBOHYDRATE SYMBOL	GMML 1.0

Non-polymers , 1 types, 19 molecules

#7: Water

ChemComp-HOH / water / Water

Details:

Mass: 18.015 Da / Num. of mol.: 19 / Source method: isolated from a natural source / Formula: H₂O

Details

• Has ligand of interest: N

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 3.67 ų/Da / Density % sol: 66.48 %
• Crystal grow: Temperature: 303 K / Method: vapor diffusion, sitting drop; Details: 100mM Tris pH 7.5 100mM NaCl 3% (v/v) Acetonitrile 5% (w/v)PEG 20k; Temp details: Room Temperature

Data collection

• Diffraction: Mean temperature: 100 K / Serial crystal experiment: N
• Diffraction source: Source: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.92 Å
• Detector: Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Apr 30, 2017
• Radiation: Monochromator: Single Bounce / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 0.92 Å / Relative weight: 1
• Reflection: Resolution: 2.65→90.45 Å / Num. obs: 140996 / % possible obs: 99.1 % / Redundancy: 13.5 % / B_iso Wilson estimate: 77.01 Ų / CC_1/2: 0.99 / Net I/σ(I): 4.75
• Reflection shell: Resolution: 2.65→2.7 Å / Redundancy: 12 % / Mean I/σ(I) obs: 0.25 / Num. unique obs: 6703 / CC_1/2: 0.281 / % possible all: 95.5

Processing

Software

Name	Version	Classification
PHENIX	1.20.1_4487	refinement
DIALS	2.2.5	data reduction
DIALS	2.2.5	data scaling
PHASER		phasing

Refinement

Method to determine structure: MOLECULAR REPLACEMENT
Starting model: 3FUB

3fub

Resolution: 2.66→90.45 Å / SU ML: 0.529 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 37.4716
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	Rfactor	Num. reflection	% reflection	Selection details
Rfree	0.2828	6139	4.9 %	Random
Rwork	0.2437	119036	-	-
obs	0.2456	125175	88.12 %	-

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
• Displacement parameters: B_iso mean: 82.95 Ų

Refinement step

Cycle: LAST / Resolution: 2.66→90.45 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	23416	0	390	19	23825

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0024	24243
X-RAY DIFFRACTION	f_angle_d	0.469	32720
X-RAY DIFFRACTION	f_chiral_restr	0.0401	3694
X-RAY DIFFRACTION	f_plane_restr	0.0036	4245
X-RAY DIFFRACTION	f_dihedral_angle_d	12.7819	9185

LS refinement shell

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	Refine-ID	% reflection obs (%)
2.66-2.69	0.6975	41	0.6183	805	X-RAY DIFFRACTION	18.09
2.69-2.72	0.5193	62	0.5217	1280	X-RAY DIFFRACTION	28.35
2.72-2.76	0.4248	92	0.4654	1841	X-RAY DIFFRACTION	41.16
2.76-2.79	0.5114	104	0.4759	1907	X-RAY DIFFRACTION	42.53
2.79-2.83	0.4211	135	0.4292	2901	X-RAY DIFFRACTION	64.49
2.83-2.87	0.4617	191	0.4334	3488	X-RAY DIFFRACTION	77.58
2.87-2.91	0.4374	232	0.422	4192	X-RAY DIFFRACTION	94.25
2.91-2.95	0.3925	194	0.4096	4310	X-RAY DIFFRACTION	95.38
2.95-3	0.4167	234	0.4132	4429	X-RAY DIFFRACTION	98.33
3-3.04	0.4608	214	0.4172	4457	X-RAY DIFFRACTION	98.67
3.04-3.1	0.4001	235	0.3892	4429	X-RAY DIFFRACTION	98.75
3.1-3.15	0.3789	232	0.3572	4446	X-RAY DIFFRACTION	98.55
3.15-3.21	0.3663	244	0.3462	4377	X-RAY DIFFRACTION	98.74
3.21-3.28	0.3392	199	0.3316	4457	X-RAY DIFFRACTION	98.62
3.28-3.35	0.3459	226	0.3203	4502	X-RAY DIFFRACTION	99.02
3.35-3.43	0.3271	243	0.3036	4408	X-RAY DIFFRACTION	99.04
3.43-3.52	0.3523	227	0.2936	4449	X-RAY DIFFRACTION	99
3.52-3.61	0.2968	232	0.2896	4439	X-RAY DIFFRACTION	99.24
3.61-3.72	0.3286	229	0.2835	4496	X-RAY DIFFRACTION	99.16
3.72-3.84	0.2963	226	0.2663	4463	X-RAY DIFFRACTION	99.28
3.84-3.97	0.2739	236	0.2317	4461	X-RAY DIFFRACTION	99.24
3.97-4.13	0.2499	221	0.2161	4463	X-RAY DIFFRACTION	99.3
4.13-4.32	0.2653	230	0.2007	4481	X-RAY DIFFRACTION	99.37
4.32-4.55	0.2381	230	0.1936	4532	X-RAY DIFFRACTION	99.56
4.55-4.83	0.2047	238	0.1855	4450	X-RAY DIFFRACTION	99.51
4.83-5.21	0.2123	219	0.1774	4532	X-RAY DIFFRACTION	99.56
5.21-5.73	0.2353	251	0.1871	4466	X-RAY DIFFRACTION	99.6
5.73-6.56	0.2805	251	0.2171	4501	X-RAY DIFFRACTION	99.58
6.56-8.26	0.2452	230	0.1984	4528	X-RAY DIFFRACTION	99.62
8.26-90.45	0.2349	241	0.1751	4546	X-RAY DIFFRACTION	98.68

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	5.17087445516	-3.13016690916	-0.909199539662	0.906376426807	1.82003479486	5.11186721689	-0.3426360511	-0.221780948615	0.181839428552	-0.0762737831383	0.269334551713	-0.546342062817	0.230910339351	-0.171270082717	0.0774501286263	0.724218808473	-0.0211881273035	-0.0350337284833	0.242433378373	-0.00104405125931	0.641617680057	-5.713	1.812	81.67
2	5.78604588318	0.380417636762	3.14540966617	1.46402973271	0.230723798349	1.40478816005	-0.109511001602	0.0227319441755	-0.799965566669	-0.502899767583	0.287270019366	0.0742595929417	0.274541290623	0.126109765464	-0.145871149921	0.713263109481	-0.0269243986732	0.0320051601454	0.382492709274	0.0218263093058	0.762389134169	5.462	-3.729	89.979
3	3.45314770727	-0.265681996817	-2.38538640301	1.50040916174	0.603870967272	4.70786374565	-0.0932465826632	-0.220906108625	-0.029288683292	0.130055762573	0.234188916982	-0.195348389168	0.28761079335	0.196903151135	-0.155090107677	0.799687777641	0.0377026172885	-0.0679366534949	0.274723465821	-0.00202219641173	0.674236566269	2.128	4.672	99.712
4	3.08377721512	2.1196390539	-2.66574142771	3.15840684156	-1.9725377751	9.22785394028	0.159268765964	0.358050821532	0.0909077848008	-0.0179141510997	0.322766131548	0.408635297991	-0.150985850593	-1.22963983188	-0.454036396925	0.665613538108	0.00309618060145	-0.0287310202472	0.665967983551	-0.071861183757	0.657598264487	-21.354	2.492	63.034
5	7.95712386656	-2.25876672138	3.44559178819	5.0961128034	-0.96126043553	2.77711497119	-1.27780127741	-0.124331524991	-0.955400728448	0.21752786116	1.46981892263	1.00440642457	1.10532679482	-1.98748836053	-0.0702975887415	1.08328731667	-0.27998966961	0.204729915895	1.1927302497	0.209069767377	0.776407960026	-23.698	1.031	33.893
6	4.20688185917	-1.62537138601	-0.353996870776	8.09988707562	-4.84258799638	2.6997958662	0.19370216319	-0.00699228975796	0.30577245735	-0.839633115072	-0.09317526815	-0.617753260706	0.0769704900944	-0.494228016299	-0.176630946722	0.661450627542	-0.0719891113607	0.0395364640911	0.471963411712	-0.0291864083214	0.631579403794	-12.227	0.576	65.244
7	3.58551611317	0.505694644416	-0.505971693027	1.41417992334	0.327353839028	2.80866411329	0.200631727982	0.189875025157	-0.229120943993	-0.137926905973	0.25617152525	-0.732313756377	0.30240361007	-0.225324406226	-0.426464987918	0.914423508388	0.127210075764	-0.0767822165869	0.411831021229	0.0717016558672	0.77393448607	-12.62	38.981	76.927
8	3.18541202851	-1.05957263584	-2.57383905626	4.81302642776	-2.88344956647	9.67712329408	0.806107433168	-0.328844137395	-0.512911544247	0.759375168158	-0.239200805462	-0.00719376954913	-2.00477571457	-0.371579156637	-0.541027021846	0.939804418918	0.033946357591	-0.140025351284	0.368386858992	-0.078279209364	0.781071003376	-14.56	45.883	90.124
9	5.39484141765	1.73264811241	0.689189051054	2.60069230851	-2.87697573903	4.91686996978	-0.706973550444	0.352154739257	0.369460866349	0.272948221189	0.330658644192	0.977912748636	-0.30550093061	-0.88680249942	0.278201077141	0.565178239973	0.0321515891745	-0.135447905475	0.448499312055	-0.0142870040551	0.730756970296	-20.352	38.691	85.283
10	6.51976703731	-2.69834718881	1.8094500425	1.50114460232	-1.62579665151	1.79896001305	-0.33447957759	0.412474490181	-0.373724744591	0.456110434289	0.0997034850175	0.410463435346	-0.0949060214827	0.00688625020188	0.225571141404	0.73466426688	0.147217069557	0.0084515629477	0.377834565357	0.0279822503879	0.803049800441	-12.991	20.534	89.223
11	2.33436198497	0.531322839555	1.95946750909	3.08592133338	0.639588085764	4.25606091465	-0.676033539256	0.57402564583	0.429132959594	-1.19112760586	0.277113912888	-0.199527607652	0.448567396948	1.49333671869	0.418945725078	1.08183339132	-0.0382280802694	0.181242908911	0.524169530817	0.142899834356	0.733372842998	-0.935	28.281	93.339
12	1.95343621653	1.63537747593	-0.615614113593	1.237236182	-0.0174830873571	7.00800463119	0.510888464648	-0.0936543390293	0.169015715208	0.32871752988	0.00731128048324	0.182070582777	0.713278435848	1.00477939028	-0.575551003463	0.625929447183	0.114457729693	0.0254478885134	0.35453634238	-0.0257464627565	0.639795568053	-4.226	38.016	102.382
13	3.80606858724	-2.48065094347	-0.466448666776	5.85906244935	2.36782374628	5.69147075739	-0.016828266483	-0.110554923353	-0.277292443002	0.777116897386	-0.939654139613	0.373482539197	1.52707201059	-1.79124210662	0.48735046813	0.900299116214	0.304248549897	0.00968291563283	0.171783229751	0.193582424978	0.617300038556	-16.639	33.161	92.764
14	2.10754483309	-1.20998589498	2.44483243427	2.02600352888	4.24677384663	2.39563138195	0.541381423065	-1.79100733653	0.28747584719	-0.782688655614	-0.288035496667	-0.166599098674	0.452596817887	-0.469460580316	-0.21746753427	0.643817524172	-0.0904154254354	0.0645826555937	0.757151019113	0.0775500396497	0.929443579767	-17.994	17.017	98.7
15	7.07235531578	0.620654261114	-0.191361876108	8.9921259121	1.36685976295	4.37838838497	-0.31611423829	-0.0910207090553	0.344560187656	0.767357096839	0.772145905679	1.36492500307	-0.284434827901	-0.478079902811	-0.500519718237	0.560768160646	0.0351971923222	0.104305824717	0.50532864165	0.0627496636365	0.411669784391	-12.171	35.407	100.625
16	5.60915973876	1.08813370569	-3.5572554252	0.312940172732	-1.64555137843	8.02627930785	-0.872303032228	-0.298739881639	0.214405506489	0.146829254783	0.133204343401	-0.439221926184	0.188959294873	0.306588971934	0.559974730076	0.871209636198	0.0675936585907	-0.00251049218515	0.387912075484	-0.0661688402984	0.78283356696	-9.486	61.906	62.258
17	2.06776649895	0.722541235171	-1.30145021343	7.41238154293	1.01024280473	5.88160022003	-0.277943546679	-0.461477297945	0.113088241262	0.569075640563	0.760795636957	0.878288246905	0.381217657833	-0.582095863117	-0.495746404717	0.843141195238	0.254180835865	-0.00806044186869	0.529232594948	0.134914209285	0.868811577295	-26.26	51.951	71.779
18	0.846568182986	0.185468592889	-1.2417195006	-0.0673841491959	0.337849130256	3.93108086854	0.68595496034	-0.0140845993585	0.393879967295	-0.426753596356	0.391293930068	0.472372249287	-0.137530369842	0.40848053077	-0.83873539671	1.0950110114	0.0951854785262	-0.038993819767	0.312627157279	-0.0341450183632	0.861718275744	-10.765	61.214	56.912
19	6.91976916976	1.66089484254	1.30890274215	7.01209786868	0.573287501687	6.7612407248	0.363423820468	0.537490893754	1.21680867205	0.20869055369	-0.00474482123841	-0.0504858687827	-1.90227601512	-1.0260744855	-0.370952336187	1.439389572	0.408168833551	0.0806464166959	0.741105424726	0.0155435939836	0.649993003251	-13.466	64.896	33.868
20	4.69974842142	-4.46916100212	3.71959724463	6.66163758839	-3.05980816499	2.17342394716	-0.223895680776	0.28387949775	0.227430185363	0.192427128637	-0.190998933228	0.137404467843	-1.21671238789	-1.17234286815	0.529382275817	0.872358906884	0.138653803256	0.195538551357	0.873199760168	0.0598867795261	0.779737106083	-16.82	58.803	41.533
21	4.56924343533	-2.28313991545	-1.91106724575	2.3058075768	-0.192633636519	5.22550424675	-0.439588310676	0.128674265268	-1.29401198046	-0.047286369428	-0.104338676606	0.119806743913	0.582054216338	-0.222006971893	0.457553596778	0.778671231317	0.100641377701	0.00470189704225	0.261220904912	0.0444322220908	0.706961739187	-19.967	44.719	72.432
22	0.188287677056	0.361567103862	-0.266163648093	2.08979847247	-0.585268314077	2.29426019668	1.45609707953	1.84573903247	0.407442057666	-0.399288640819	-0.242057752196	-0.0532217322546	-0.0426488431725	1.492344294	-0.396853398592	1.0001901228	1.38257821694	0.271729811102	-1.37003336671	-0.204284229404	0.958154193418	-9.927	52.544	58.508
23	8.63823590073	0.976593027505	0.0711144447782	-0.284653129428	-1.60224915699	6.01839225911	-0.114692056675	0.0998438933149	-0.265066189124	-0.162989978908	0.113644121146	0.215629833088	0.14656307258	-0.0494031050152	-0.0081570226077	0.670733104804	-0.0823533175738	0.0190156023599	0.24943110079	0.0518053164357	0.692188519517	21.387	59.87	82.243
24	6.74555657972	8.09083540829	-1.67482459897	2.05046468027	1.70192352521	9.4533372366	0.433907605251	-1.52880122479	-0.0537394876967	1.90920452724	-0.912735182658	2.04029964935	-2.50476964627	1.63241454578	0.405649860221	1.27148143441	-0.118734221194	-0.0843799789348	1.07276076644	-0.00352443766127	0.694043782951	21.946	68.395	99.81
25	5.10708486147	-1.3238502112	-2.39677150934	4.03222604163	-4.05265518293	6.95302401793	0.611293532973	-0.391760377436	0.501948120211	-0.0158689173603	-0.225455784205	-0.416010897405	-2.23423707159	-0.371611024004	-0.358016266304	0.951849986861	0.077033418481	0.0421708477554	0.332370842898	0.0389155220002	0.824245698821	16.464	66.316	89.675
26	9.6949163434	5.50477035512	-3.39186387237	4.63628634477	-1.24255281578	6.04638042407	0.857338763249	0.538019357607	0.852716734969	-0.0601866073726	-0.631338040297	0.250409117374	-1.65623805944	-0.157418455541	-0.188286347932	0.787947267587	0.06748168182	0.00680525245409	0.496523719836	0.0533908329117	0.57404694253	1.52	52.997	90.902
27	2.17620529767	0.190485587072	-3.41027243582	6.34171916886	-4.60492655699	6.57652239406	-0.34185244305	0.319328675654	-1.20405186642	-0.853990524234	-0.11990219707	0.366925302345	1.64493531986	2.19729870888	0.57436213615	0.65589170936	0.143266764816	0.0697856598261	0.630898089698	-0.0742451536389	0.636129269506	12.647	43.676	94.801
28	4.69221431451	3.49337468777	5.02111918589	8.06028941945	3.18847179266	1.97956673805	0.591018981698	-0.274619908764	-0.565418232525	0.476500073057	-0.59177152976	0.110356990462	2.14941482693	0.00972468756027	-0.00852611777234	0.676011142158	0.00993375295725	0.0403378955507	0.546912598429	0.0302569316479	0.505088263631	19.829	48.008	110.562
29	6.97668164206	-1.82200776144	2.83603188576	2.27613662742	1.8070927804	4.185856514	0.272982214411	-0.438231575164	0.014242241022	-0.262218544703	-0.286981243142	0.0731823635284	0.0279793533052	-0.323516068514	0.0218766952504	0.829633675601	-0.0457551995066	0.135178338729	0.46649914079	0.0940327210775	0.683832395919	11.653	56.617	96.974
30	5.87681163722	-2.76167471482	-0.510885997531	7.06021510537	2.6176482855	7.37470383873	-0.307984308292	-0.480184077626	-0.441313363446	-0.77947081931	-0.150369852322	0.2866562723	-1.08035182699	-0.463830955506	0.450142323095	1.08033317935	-0.0148777205506	0.0598720910554	0.500609166192	-0.0164050753166	0.568222189888	2.609	52.505	104.487
31	7.60230190001	3.62994775423	-5.51128968788	2.91361301892	-1.54135567149	6.49810196587	1.21123953365	-0.133609122258	1.98113023593	0.301169041187	-0.294194487291	0.189170816915	-0.540319210929	0.331044687819	-0.811216954837	1.06541989418	0.0422055012598	0.157561609365	0.569715265758	0.163948987361	0.847904702974	23.617	58.227	103.221
32	3.21187651687	-1.66179494474	0.632771346011	5.31486477358	2.15778520094	7.21293946057	-0.214060345756	-1.51462477194	-0.515215920969	-0.180898867343	-0.872878125656	-0.437387335252	-0.0672613923908	-0.0354793241866	0.933140480899	0.945464043308	-0.367826246773	0.0773411433989	0.849044745128	0.106573406849	0.934638294737	48.172	61.492	57.658
33	0.740662375722	0.11906871208	-0.861475121041	0.10039765751	1.41407896668	5.70790041926	0.388852413543	-0.147155283558	0.320120086309	-0.00655166334767	0.0769597256812	-0.093723875077	-1.46136300645	0.664986533081	-0.388213707886	0.903932839683	-0.135875264269	0.0475798247651	0.437223940307	0.0248449675601	0.771104182788	39.442	72.202	62.502
34	2.3130277138	0.841187995285	-2.54158229278	1.58154747067	-0.506685789659	4.08039860691	0.329698017693	-0.196393327911	-0.177695857852	-0.200022568131	-0.230971889952	0.0371980112785	-0.651191746903	0.178042022887	-0.0789829671896	0.825915397427	-0.19782667545	-0.0364246267801	0.44346326995	-0.0121468926327	0.81023963126	31.734	69.982	63.016
35	3.1578820025	2.23220156116	-2.01364422668	1.56071559272	-0.17391460572	2.07215513613	-0.211890601652	-0.149613841355	0.0463354523916	0.329172027158	0.257085010296	-0.0876868588083	1.01150729217	0.301978403218	-0.109853922658	1.16948792434	0.245141713023	-0.0111049664557	0.433877524537	-0.00164453539589	0.761808621819	31.36	-7.656	83.378
36	3.93057990192	3.66298279194	-1.81282487987	3.38246761154	0.0323325529843	4.53314109674	0.301656115843	-0.0298876267907	0.971031103227	-0.045778600458	0.0654271215151	0.495829514479	-0.720026504251	0.0883933820689	-0.329510062627	0.769892380054	0.0816741886639	0.032812269799	0.423274027621	-0.0895300560396	0.776768824207	36.008	3.071	83.52
37	1.68331140882	-1.93814979861	1.82544100701	2.31940009317	-2.35403196381	2.90717424193	-1.25283811839	-0.640931684166	2.2789992251	1.94315460271	-0.286445384155	-2.68267423556	1.11064962038	0.980169078848	0.934816321933	0.766263707856	0.238765879135	-0.182124434205	0.843886807229	-0.0272441067177	1.00473917077	35.405	-9.333	100.869
38	3.52260312677	1.12320784312	-0.139540204977	5.36007728264	3.225088094	2.79249641458	0.158446199927	0.15077055767	0.154893762147	0.22939693611	-0.228069743157	0.182393769139	-0.31943805665	0.270444912631	0.0586033930738	0.656480585201	-0.006509088302	-0.0476100040702	0.406973604503	0.0420166617528	0.508976162826	38.758	9.172	94.828
39	0.307262674034	0.232952445204	-1.43924491226	0.936417203329	-1.57699867995	4.84573493739	0.357053390951	-0.267434562067	0.31275741082	0.187149418195	0.110029533352	0.213909990725	-0.924113474141	0.321324421887	-0.495062178147	0.697447611599	-0.0465294533829	-0.0159357482875	0.375575916968	-0.0706010376632	0.637988856793	27.998	6.749	102.493
40	3.88662820325	0.549364101245	-0.914706704011	0.841592219124	-0.301329007033	7.44120932758	-0.379192250629	0.30230365839	0.00136171359573	-0.560011917618	0.224965141954	-0.753537056467	0.427989106622	0.54277973398	0.150850241101	0.531700154148	0.226336298302	0.0146168706027	0.917364140929	-0.0124208320555	0.825653476455	43.928	5.158	103.739
41	4.92258222012	2.76303671691	0.808369617267	3.89818028733	-0.776646398347	2.41049818638	0.856891215502	0.118978468556	0.301276956845	1.39827053391	-0.487927124249	0.409607431131	0.217731763196	0.302699371637	-0.299171563428	0.863605464436	0.194206899658	-0.0297275405534	0.518280610218	-0.0882383054133	0.615787649933	34.345	6.025	106.28
42	1.88589922968	0.61607184067	1.2439727585	2.75597662821	-1.11002319647	6.57288160251	0.200102593538	0.140909060702	-0.533763237714	-0.200324338214	0.356626716396	-0.068737000133	0.949191801273	-0.0647535439886	-0.542774011002	0.755710053179	0.099066811331	0.0979109858077	0.462280858422	-0.0206307218397	0.557090845363	29.1	-19.567	68.693
43	5.40310388129	-0.794625757753	3.85171359625	7.44271982324	0.972202000598	7.17551907977	0.464774384563	-0.0696328987823	-0.996678979792	-0.550110620016	-0.21922983064	-0.210408442666	2.31799397772	0.694001689712	-0.310409425145	1.1842714729	0.11897460686	0.0223366295735	0.629552375227	0.142819376563	0.741387189741	32.945	-25.427	40.408
44	3.11400769722	1.5798977002	3.1090918171	1.19068192357	1.07804748856	6.98878437483	0.0542204457427	0.0602195396789	0.0997772315377	0.400058192349	-0.141603019684	0.376574122446	0.463241933311	-0.119936508407	0.111427587195	0.841850418866	0.0320224221564	0.0598794337188	0.411072973832	-0.101348952411	0.732111261066	34.258	-11.302	70.961
45	7.42915007411	-0.309482580704	-0.86475898584	8.6193216718	1.98717705398	2.03578411789	-1.32449367608	0.389333543284	0.0936482116734	-0.797944118428	-0.0124294536763	-2.50761525453	0.808357534357	-0.0815904351899	1.24577432079	1.08911407167	0.160125552223	0.0651529522058	0.533793038274	-0.0105061811013	0.673510161813	-5.394	55.707	13.066
46	3.25232881477	-1.7662449221	1.83031529532	1.81493219643	1.21620805247	5.45271387941	-0.383594945467	0.441035984281	-0.214350737761	0.307305997103	0.516064092553	-0.0871461766575	-0.432878668014	0.465893288634	-0.153131153197	0.983707621252	-0.0249545177014	0.0325248770367	0.374845160056	-0.0315047252746	0.706589148198	9.295	57.964	24.05
47	3.9811512419	0.574368101561	3.05118688857	0.986996854639	-1.41127319058	5.86849493462	0.946666834107	-0.166670824121	-0.514581184925	0.635725290348	-0.349396023132	0.0908821439391	0.218428773515	0.213180422307	-0.513753768522	0.947324778193	0.0263746460908	0.0862042144719	0.589413604523	-0.0966927401871	0.76347415638	12.895	50.338	19.796
48	8.020933343	1.02117440545	-2.57599483081	1.70253369533	1.4918818776	8.59396889523	0.326542121145	0.783218819245	0.130650488522	0.525233610526	-0.491538367611	-0.24473992055	-1.01762245981	-0.786338755417	0.145302801174	0.8148525282	0.0812032164872	-0.00670646402943	0.471887557553	-0.0820805951824	0.64056956469	4.215	57.404	8.932
49	7.20542240722	0.197519668073	1.02070600509	1.55486088354	-1.36740408355	4.42537565281	0.130513472884	-0.533320467821	0.259239042228	0.97637298123	-0.368427500611	0.19349223482	-0.0367841680417	0.206118934856	0.160925475258	0.888734278876	-0.0546734234313	0.0207600005708	0.481059879546	-0.0192153731173	0.560209704593	24.32	51.41	8.793
50	2.13215265764	0.458872109774	1.86849628835	-0.0946353709046	0.474269827623	2.85850112188	-0.0650372470242	0.036522419205	0.190721946401	-0.0811009800521	-0.342015317024	-0.0693065187496	0.439727004281	0.127472357754	0.37641229682	0.704004912077	0.00566254786048	-0.00466778203335	0.408135765139	-0.0782558505255	0.6113231518	12.251	44.218	-3.539
51	4.89342594153	-0.485860406384	0.630877851169	2.94374985498	-0.741538734671	5.88916543083	-0.0673368993982	0.316652797298	0.923513073989	0.0642434095408	0.175689164622	-0.305122017345	-0.698803791991	0.0632622101239	-0.169047893413	0.852070948983	-0.105182919619	0.126367222793	0.475812922619	-0.0644851259632	0.713718766851	14.197	58.317	4.796
52	6.50560831228	-2.49489013874	-0.489041912878	1.72805170399	0.854634390014	4.01331873736	0.512607243306	0.862406254256	0.482125829046	0.422716834361	-0.142339912588	-0.0288912166851	-0.662690414409	0.295385993915	-0.413054141866	1.03924657818	0.040425897719	0.121595347909	0.379671316722	0.0873803402096	0.604517635727	18.491	54.908	-1.974
53	1.99563660325	-9.30444230285	2.01092471678	4.98663393796	-7.87138383001	2.00115643023	-0.224441572544	0.215553607467	0.42259926809	-1.48588915993	0.0925437883027	0.536916918673	2.14130770327	-0.660900074839	-0.142208799981	1.15979050178	-0.00948140962039	0.198257410037	1.50256307574	-0.140870048384	1.07119203238	-19.47	45.17	43.194
54	1.66220350642	-0.699289015281	1.41424691983	1.79148191893	0.398981646885	5.87618631333	0.412857658626	0.761438383722	0.369809851964	-0.14828481378	0.112936634453	0.385675622039	-1.04240864014	-1.64196469143	-0.466447837364	0.772598382735	0.262984818761	-0.00100019988924	0.473244005218	-0.117981169781	0.803912366474	-13.932	57.678	30.314
55	2.19187482098	1.13955724606	-3.64676889875	-0.421805050096	-1.53676706828	8.24001982771	0.0558836170066	0.361554838065	0.0825744248911	-0.146681571525	0.0949618206296	0.00954126167044	-0.0170641433094	-0.697874566223	-0.142441153627	0.923456665083	0.214532872055	-0.0466112098804	0.425943940801	0.00834827386873	0.663076198698	-11.732	58.427	42.566
56	4.30912626149	-3.99373406058	0.773516200774	7.08359297434	-2.70362152728	8.6835950501	-0.0744393161725	0.235382201132	0.241995037603	0.173322642189	0.0678974429649	0.504866877991	-0.438385449879	0.563233801505	0.0332879783423	0.43666674312	-0.057941819703	0.0194828426097	0.583818570433	0.100225444656	0.665437416541	50.633	35.073	83.996
57	5.96633183503	-5.6852277263	-3.12828836471	8.86377647571	2.61253880571	6.63329515995	-0.492135220721	-0.663751906382	0.263176251266	0.702837243357	0.102120601463	-0.0403888377831	0.312645899101	1.0553837323	0.341648758648	0.6777024688	-0.0884972620535	0.0705169928969	0.653463332637	0.0592292924816	0.62938423677	51.799	37.673	95.362
58	2.08839245737	-0.665876291591	-0.0626489417493	2.08123631088	-0.760549949034	2.0507729427	-0.0742463272764	0.0560704788408	-0.0667651838353	-0.0121217809353	0.0286752877704	-0.268551864587	-0.144424858573	-0.234029760318	0.050055289583	0.977559794454	0.0134239357113	0.0657190191998	0.498014130595	-0.028519490354	0.721262370207	35.925	35.893	100.424
59	6.43827374284	-6.53705135179	-0.157214045941	6.50430710117	-0.146105703505	0.969080288056	-0.987122979019	-0.408403270352	0.524754611419	1.0579634077	0.68830875238	0.00195608529619	-0.187611868736	0.241268188569	0.323440977843	1.07479711363	-0.169170962962	-0.00992400850741	0.753005651931	0.0808005423492	0.924754675435	44.344	47.476	104.183
60	5.229470738	-3.08052180916	-0.574306323229	8.70898287754	-1.43596668799	4.88847366599	-0.280546834402	-0.798994169814	0.0641969787499	-0.0645793998521	0.74573037418	0.147404271946	-0.274549903608	0.288347767181	-0.465245736818	0.86072105071	-0.117564780481	0.0426633702921	0.602286224075	-0.0303955811599	0.534335730202	43.015	36.308	106.518
61	4.09675386671	-3.10330635646	1.51018298692	3.76379734628	0.499078954254	5.64394151314	-0.424967892744	0.195639187097	-0.199690834263	0.21787514089	0.385431732523	-0.381340964467	0.764707289481	1.34935317754	0.0851490714704	0.771984087467	0.110555750619	0.165455415718	1.19547937931	0.0848947774956	0.750628672015	67.32	24.819	77.213
62	2.54915705475	2.35827403467	4.80476183109	3.39011265978	4.30501663249	8.99800913011	0.65643630983	1.40310439774	0.365252197771	0.950925337568	1.59871054217	-0.371248466598	0.689551940515	1.02592363466	-2.16593967904	0.801284115866	0.23826220174	-0.0246009217137	1.18693564303	0.13736886944	1.49456699304	68.003	12.383	53.927
63	6.38789566712	3.599195359	9.31083031757	7.14765198908	3.1172948957	2.04817256944	0.721911539321	0.627735932891	-0.897306593802	1.31412142294	0.283967752143	-0.218811314642	0.907709119139	2.36924208345	-1.22951016028	1.06650707949	0.294649974606	0.213844604364	1.48675952298	0.173981828456	0.830620686507	73.916	17.265	43.656
64	4.49762417357	-0.318475373734	-2.52692600981	3.13073284223	-0.183523798968	7.37418139603	0.0910603733311	0.584294247159	0.135758786138	0.150487281573	0.45165343205	-0.300191425123	0.578275568728	1.16281001328	-0.52607119055	0.897700930041	-0.0549122201379	-0.101338906878	1.26425317327	-0.0762843472208	0.85935314617	73.056	22.973	50.25
65	3.60268280551	-2.68577160769	-4.42179043787	3.66297261778	0.389454262095	9.93529256779	-0.496617331137	-0.192554332924	0.323463470173	0.0874982617334	0.0876020596632	-0.227972549455	-0.341372224176	1.29150939555	0.344259394531	0.541945788895	-0.0910294666238	-0.0191083872293	0.91242361807	0.137722220616	0.766235388935	60.347	35.31	81.132
66	0.358567487017	1.40430218962	0.585422967812	8.90753961142	3.73152246367	8.548028589	0.152572577146	-1.34580148084	-0.315326856149	-0.365038369176	0.504824076628	-0.698544051003	0.807838973652	0.662761261188	-0.516594547925	0.738380277143	0.179859947788	-0.040189527202	1.19215756299	0.0631094422694	0.819647781476	60.163	23.723	66.192
67	2.86061637743	-1.6961985391	1.96810272591	1.1666321342	-1.91906620082	3.50191374693	0.00014603450162	0.00824569032214	-0.220900742938	-3.04824803304E-5	-0.119622263328	0.02530161172	0.466136373609	0.0114092793604	0.113305710857	0.807610267579	-0.00781313860699	0.0873832616866	0.372030461429	0.0108358753759	0.561161561776	20.013	-3.711	14.823
68	2.85280236627	-1.17273540725	-0.772517040043	2.3102089648	-0.299652736394	3.29344133715	0.0287574702908	0.147817752019	-0.234052675297	-0.355177161141	-0.137974384407	0.0602057295069	0.352120041287	0.0575838959638	0.0670592928735	0.905364736981	0.0142498167325	0.0211622837507	0.456362521365	0.000144719591781	0.610909785933	20.77	-0.16	-0.164
69	2.03077434024	-1.91651229451	0.915880265025	1.30762504219	-0.798797824112	4.40963284679	0.334445520892	-0.0841555136921	-0.443981798471	-0.0288876506977	-0.09003033566	-0.0795640808872	1.03242997027	0.312873100598	-0.273636892527	0.939600113001	0.0619604927494	-0.0214775522119	0.509582586971	0.15870366439	0.709765034136	35.238	-19.66	36.109
70	3.71202448116	0.893290961166	2.84839582151	6.88198270566	-1.72084026327	4.87129300738	0.134588969511	0.510764172928	-1.13048835569	0.460018713514	0.0724479787313	-0.0838789510646	2.16495629849	0.428651351753	-0.189976616235	1.320414417	0.104109059642	0.177531556869	0.578409993724	-0.102556422601	0.858148042245	33.738	-24.395	64.955
71	2.84781897186	0.631593718878	2.95057582026	1.60848359456	1.26785875707	6.80179880498	0.254409692171	0.139680064817	-0.0525625208139	-0.344011856262	-0.0206771070369	-0.0694749335098	0.178202684297	0.246615025415	-0.26911069673	1.027412958	0.215129414215	0.0181809976897	0.474350226226	0.0726908722569	0.749155230738	27.456	-14.003	33.843
72	2.84510387705	-2.74476969878	0.0742410267417	2.47657338504	0.376969319086	3.8319466652	0.482199121622	0.182385296764	-0.105165925507	-0.543424990219	-0.393185729788	-0.254599736803	-0.820523318789	-0.218321797554	0.00744366172357	0.967832314313	-0.038402288459	-0.0127018169491	0.370964760991	-0.0647973289956	0.822964810402	41.544	59.612	22.679
73	4.89872039745	-5.24318579518	5.85266358147	8.37601029585	-4.11325502694	2.03312006852	0.376661332726	0.849631486758	-0.529254736398	0.389524624568	-0.940185960015	-0.497719030072	0.527535057976	1.48338898113	0.456125764811	1.02181819176	0.015152146726	-0.016859937136	0.932987577495	0.121774275845	0.72610047998	56.824	45.501	30.573
74	6.11963427641	-3.30521789062	1.61608316514	6.17735817173	-0.67478045899	7.08743370936	1.0215896417	-0.528667877393	-0.801731328828	-0.476556218462	-0.310419289266	0.585452384705	1.29769808178	-0.511967187096	-0.649671314163	0.882393464826	-0.0863233994178	-0.0782293731157	0.581005916708	-0.0670010805825	0.793892319934	42.499	45.22	23.278
75	4.90375506683	-2.19468358215	-4.1259625422	2.41113642427	2.11016299437	8.87143632369	0.334155271739	0.684071136227	-0.77042645802	-0.477985324318	-0.612505453119	-0.109480750193	-1.9061686226	0.0644779005765	0.461434430369	0.972766325527	0.0381338891461	-0.0892487335179	0.630001658656	-0.0384224084473	0.711046933535	43.502	58.038	11.336
76	4.9016419979	1.98090796179	-0.660308042786	8.55247871271	2.9872827761	3.91990764959	-0.107932911093	-0.351661521393	-0.438059886083	-0.861882275267	0.0628785165945	-0.0700748110637	0.539951945933	1.08988311391	0.0918143295806	0.775988067722	0.131469346395	-0.0332901305186	0.562663347877	0.0714834529226	0.554688048163	53.676	43.646	14.593
77	6.53882290307	0.315161707818	0.857286034614	7.55255862404	-2.17419054979	6.10598737414	0.160542649681	-0.949629441048	-0.242325443361	-0.11849447752	0.0108635517226	0.672562978912	0.0733046721686	-0.761446127048	-0.13448263535	0.78742494293	0.0947645369566	0.137282693113	0.741176911925	0.0592275910546	0.733330331459	40.546	35.962	8.878
78	0.419157044359	0.314571577507	-1.21915464188	1.6426981676	-0.747255885199	7.19972769978	0.312273553224	0.216538157608	0.0729677395362	-0.145693276137	0.211997029508	-0.193351191852	1.07092290434	-0.659277987762	-0.520621064272	0.705485807559	-0.0163446504756	0.00600756185558	0.598993165402	0.0238329217543	0.726548544175	38.907	45.767	-0.595
79	6.61025960765	-1.99647475695	3.09474454648	2.29995977562	-6.21945590607	5.58070718407	0.235271935389	0.0383396389036	0.417432456324	-0.830472351474	-1.33131373399	-1.12529739234	0.192410015143	1.0715419737	1.0117502213	0.745633652821	0.139458178599	0.000228325253812	0.703098661115	-0.060392942466	0.68749625344	51.788	48.011	9.182
80	8.11801313862	-0.0683480251763	4.27977808415	4.87835830117	-1.4365990416	9.39269453849	0.0868382910016	1.14178929778	-0.386443344415	0.015454226119	-0.304461501287	-0.541111793314	0.143201767574	1.12752350319	0.178600053427	0.749759634687	-0.165702823112	0.0472913170367	0.715234517764	-0.0627554320638	0.760763843923	56.019	37.208	1.293
81	4.98306889455	-2.96567651191	2.76612064184	2.2049225372	7.32464243453	8.73602716454	-0.275163123242	-0.0822491623985	0.607274125114	-1.99728455707	0.700932171939	-0.383490785822	-1.23386169825	0.474871648884	-0.296884436679	0.767433001714	-0.0761866752871	0.0381040035303	0.610349805413	0.0112384079481	0.604599005045	41.395	55.81	2.605
82	4.19212259001	-1.03490021056	-0.125093586051	3.45140029915	-0.714850473519	3.51008357039	0.234205977975	-0.493534545227	0.0492318520607	-0.0558570486022	-0.201718863062	-0.376120446057	-1.07279768821	0.22850924054	0.0097038557643	1.02898977591	-0.148787660247	0.0169236359195	0.639218258038	0.0430761857595	0.644382271149	43.58	71.398	31.274
83	2.53931245356	-0.83424712159	-3.37500259241	1.29927501143	0.244585053017	10.0999914455	0.91003177398	0.248260802701	0.271768406061	0.0588160957274	-0.0936608866267	0.0285563952076	-1.70926229353	-1.07621345922	-0.945445146232	1.09931159785	0.0339330929118	0.00035653341325	0.554190052233	0.0436495035301	0.846650313986	33.059	72.533	43.506
84	5.7047398327	-3.90983851651	0.895432625701	3.16169634353	1.70648865179	9.52256439396	0.931610097349	0.463737432642	1.36126281767	0.110829609455	-0.258689441489	-0.810047405457	-1.7264310983	0.454354984366	-0.667938859307	0.935417066574	-0.13577463155	0.00891478859121	0.555027436571	-0.0281480648125	0.852456284684	33.723	77.881	66.325
85	2.97084985784	1.56430899408	2.39074472792	2.77140190609	3.05775594338	3.48939836517	0.317167448542	0.20551267194	0.63209319875	0.0274219658776	0.000470047189543	-0.745253509976	-1.82677455786	1.31191263873	-0.17603269965	0.824243538208	-0.386881411234	0.0986375766874	0.822746928889	-0.0148389677998	1.01249632271	40.784	76.929	64.061
86	1.18778586888	-1.5474543314	-0.77257090571	2.71840596343	1.4505181687	3.40415052142	0.464939440568	-0.12045550055	0.168928422148	-0.688732730622	-0.0408442888176	-0.385807349111	0.0774213335578	-0.00367208978923	-0.419345615656	0.879391818726	-0.0733549480893	0.00220227754645	0.389013676692	-0.010370180868	0.709586109711	42.759	62.691	35.572
87	4.12967896073	2.43252587001	-0.02394255432	5.25613233561	1.36262930789	9.63331526785	-0.124199446082	0.0178774250849	-0.403732401862	-0.298530756378	0.00724706777081	-0.0988651524336	0.228253002654	1.21927478951	0.145401599332	0.589751311305	0.105611323782	-0.0183652715728	0.630684280023	0.0751497200185	0.606363137268	57.572	14.701	21.439
88	2.2727047049	0.519630889567	0.0549211385621	0.456267049927	1.49958100988	2.2736120743	-0.141659349673	-0.162288548024	-0.127984625655	0.176503555643	0.124482243348	0.0298357246894	-0.065868508752	-0.0931060866038	0.0490135135161	0.799464048693	0.0217906302538	0.0175452184485	0.518662557976	0.0127144109397	0.692675031477	45.516	12.871	7.622
89	6.8850338288	2.25402879548	-5.02042853705	2.6277243724	0.294175177446	5.01223939619	-0.816379176921	-0.38261815632	-1.10537069837	-0.821770670136	0.0259458787639	-0.290686772607	1.04144551275	0.437270935075	0.613823461817	0.860519839904	-0.0278180607645	0.0218012204847	0.628247695277	-0.0225910032221	0.617767154971	53.95	8.191	9.485
90	6.5569516565	3.31741211166	-0.668825633792	7.16646954267	-2.40153825484	5.66660432674	-0.176143568065	0.217194290841	-0.132909457731	-0.329765630389	0.26989819976	0.146836669536	-0.150261097113	-0.137119506809	-0.0917001018412	0.795782463232	0.231833659606	-0.0163277972453	0.608924497195	0.0200150400906	0.627897382713	50.498	9.415	1.74
91	6.21900657018	1.24216136694	0.956652917909	7.34258014954	4.88495251583	2.2843817703	-0.132488418524	-0.0694868064836	0.0992591300444	0.322348345137	0.554154483911	-0.51045961163	0.67783578225	2.33683536994	-0.46561433937	0.813254153693	0.0796949541634	0.0322190178802	1.20874715129	0.113375974937	0.617871456496	71.545	21.639	35.49
92	2.84092447016	3.17370856484	-0.997710975607	9.60745456791	4.28043810577	5.55118001212	-0.0263463109049	0.231389715273	0.360627926823	-0.968868152378	0.359732580532	-0.922113024609	-0.87056744069	2.43220533751	-0.375912636803	0.632890104873	-0.167541496088	0.0378916908442	1.25176600873	0.0464047332442	0.749571611368	71.279	30.81	66.268
93	0.888269032575	0.940851705386	0.43606620042	4.01881161155	0.674625511007	0.214345244244	0.114059259474	0.426545573881	-0.0505011107407	-0.731253317367	-0.762023654112	-1.46221531753	0.851515463508	0.281166409503	0.607473624431	0.738752006762	0.105633965325	0.161669879867	1.18564743794	-0.0570754783785	1.18646737727	74.483	21.641	67.807
94	2.37932389143	-2.10530666061	-2.08245969505	4.42553159106	2.85399634911	6.15950973371	0.227523976305	0.100416853645	-0.179812141108	-0.187431242263	0.0479818910591	-0.0323420629733	-0.393548904428	0.324400869005	-0.314724147568	0.564181516231	0.0577309350863	-0.0272230682109	1.03460639959	0.0755044731875	0.811445930802	63.497	18.052	37.72
95	6.65960717251	0.302078774379	3.28230104634	8.09305637095	0.69392180412	6.58552000816	0.130646667938	-0.115742417768	0.40941846632	-0.129157417453	0.132189705956	-0.136027620194	1.09000741182	-1.01289247343	-0.280032331787	0.702119339021	-0.0700107171623	0.108426066117	0.609096800544	0.00721945484183	0.474518573749	-9.319	12.091	17.523
96	2.69121166464	2.04986462997	-1.94789802705	6.05670688118	1.96550558787	8.62879782972	0.112119675159	-0.0590357946434	1.05458914902	0.113137961463	0.0130419621178	-0.314181478873	-1.13702842942	-0.787020401014	-0.324110947164	0.780032004829	0.0989806822471	0.0876036053739	0.533586936493	-0.0601533501632	0.633530554379	-6.289	27.114	20.205
97	3.53032783249	-0.84477505602	-0.531440817087	6.60086755063	-0.362456388764	5.87605914097	0.10225477455	0.074695920862	0.0916643735032	-0.728521181788	-0.0481185767033	-0.25128025919	1.01546596222	-0.682830409638	-0.0742976198419	0.896040995797	-0.0402287711641	0.155114405137	0.485799097993	0.0179680277296	0.603517538042	-10.416	16.014	6.95
98	4.26267568801	5.97369693559	0.340584972086	8.10263159276	0.540301010312	5.36121267683	0.175269236869	0.0274555021596	0.149335833442	0.0279841465153	0.253262228347	1.33661878661	-0.84972637623	-0.220949005787	-0.328827522822	0.96769981853	0.0885427872711	0.0736893499238	0.413132976628	-0.0053835505007	0.904110984223	-3.152	37.572	6.922
99	5.55237522856	-2.25350227138	-5.10009522069	6.68462466978	4.37252766166	6.60444948653	0.33266903558	-1.94011203667	-1.53082061917	1.65339864294	-0.00599076453834	0.309464367791	0.372772738425	0.989735362927	-0.428343924831	0.537371617917	0.305986053657	-0.0742527056324	0.389750553079	-0.0164044353455	1.01822463771	8.341	28.073	5.422
100	0.586531378261	-0.600213539434	-1.33086656232	1.9634473505	-0.111123233588	5.02440089188	-0.0239067602089	0.0456510090031	-0.126639110149	-0.797044260762	-0.126493285015	0.346071916697	-0.163616958801	0.451740394327	0.0589392005876	0.701821152885	0.048423803425	-0.00817452817298	0.348168995124	-0.0281697464091	0.590784372573	5.817	18.668	-4.182
101	6.22635705497	2.5073605364	1.30548136125	4.4555481335	-3.34552238966	5.52665277228	0.614433125399	0.224850210591	0.454452400396	0.0824839843158	-0.681483159526	0.747484313312	0.47083119242	-0.0400466555245	0.0259996490534	0.935182764187	0.0893471487127	0.0835664466448	0.367533708876	0.0111471927852	0.584091402157	-7.733	24.901	2.831
102	7.67261258371	-6.11480184068	2.85169577479	8.09507848202	0.661788005347	5.72526993011	-0.086925665323	1.1534901965	1.83249532858	-1.00530406228	-0.74909892707	-1.74299694992	-2.08693735268	0.955680222005	0.603299238429	-0.02570575218	0.899134304307	-0.0967260745207	0.773983245346	0.400835083808	1.20145615187	-6.877	36.669	-0.692
103	2.25426376804	-0.0013859111559	1.78558698953	9.49614745198	0.73546600063	2.18981518769	0.254911436035	0.20775762551	1.08943003246	-0.909338594101	-0.435388159029	0.677373465774	-0.885433412348	-0.663459801654	0.0875191567782	0.879949868835	0.00736289642286	-0.0296911560682	0.737882199701	-0.00869462256729	0.672495689836	-0.903	35.806	-6.254
104	2.13388084777	2.3582595478	-2.44504338953	5.81286863045	5.06897685368	6.92372248838	-0.306148852829	0.192036871202	0.349591136932	-0.416530439671	0.633424668424	1.3725212317	0.313892942458	0.158182145167	-0.212966181483	0.589097672512	0.00149949568039	-0.0522327949744	0.455641956379	0.0306682827388	0.574805667556	-2.49	14.291	-3.05
105	2.28095611813	1.21652782462	1.90356175345	4.68772727111	-1.86896205603	2.67706156667	0.21078853655	-0.0625266232035	0.0304599324562	-0.588981088855	0.543179920566	0.298924548176	1.77956132556	-1.34425901466	-0.750569537906	1.03707263999	-0.0835990565059	0.111624139872	0.871011726214	0.0802162254034	0.725278222836	-17.109	0.859	30.427
106	7.07646435107	-3.03395167821	-0.154442349836	2.21697897453	-0.548934162331	2.06225758804	0.473217827152	0.376438713566	-1.70654367489	-0.89514603412	-0.40203085012	-0.767326656867	2.12227167806	-2.44927532269	-0.149643816814	1.1300875387	-0.30702647206	0.0275548834709	0.68240694537	-0.127133227021	0.791750636924	-21.388	-5.607	60.277
107	5.48469793822	6.04330776723	-3.223695589	2.14546116122	-6.80581638405	7.08059272215	-1.22787988109	-0.534301821592	0.747003782506	-0.935067169708	0.18267265401	-0.0359749170224	0.282227457812	-0.582428329352	0.925741267734	0.911838897243	-0.109348392627	-0.163440573801	1.0515844824	-0.048445413323	0.74515535097	-26.366	-0.903	56.853
108	4.78329640185	-1.79809230064	4.47428092166	7.42137362526	-4.68057044403	7.17967321292	-0.181857485581	0.152600016348	0.0569479517881	0.589891910217	0.270908346327	-0.132334742528	-0.676601457045	0.0684622447667	-0.0976328849449	0.715203193824	-0.118822414091	0.128217011246	0.683315424537	-0.0715085505811	0.554137125592	-14.402	10.227	29.307

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	( CHAIN A AND RESID 149:222 )	A	149 - 222
2	X-RAY DIFFRACTION	2	( CHAIN A AND RESID 223:256 )	A	223 - 256
3	X-RAY DIFFRACTION	3	( CHAIN A AND RESID 257:355 )	A	257 - 355
4	X-RAY DIFFRACTION	4	( CHAIN B AND RESID 139:178 )	B	139 - 178
5	X-RAY DIFFRACTION	5	( CHAIN B AND RESID 179:201 )	B	179 - 201
6	X-RAY DIFFRACTION	6	( CHAIN B AND RESID 202:235 )	B	202 - 235
7	X-RAY DIFFRACTION	7	( CHAIN C AND RESID 151:202 )	C	151 - 202
8	X-RAY DIFFRACTION	8	( CHAIN C AND RESID 203:222 )	C	203 - 222
9	X-RAY DIFFRACTION	9	( CHAIN C AND RESID 223:237 )	C	223 - 237
10	X-RAY DIFFRACTION	10	( CHAIN C AND RESID 238:256 )	C	238 - 256
11	X-RAY DIFFRACTION	11	( CHAIN C AND RESID 257:269 )	C	257 - 269
12	X-RAY DIFFRACTION	12	( CHAIN C AND RESID 270:295 )	C	270 - 295
13	X-RAY DIFFRACTION	13	( CHAIN C AND RESID 296:314 )	C	296 - 314
14	X-RAY DIFFRACTION	14	( CHAIN C AND RESID 315:325 )	C	315 - 325
15	X-RAY DIFFRACTION	15	( CHAIN C AND RESID 326:354 )	C	326 - 354
16	X-RAY DIFFRACTION	16	( CHAIN D AND RESID 138:150 )	D	138 - 150
17	X-RAY DIFFRACTION	17	( CHAIN D AND RESID 151:162 )	D	151 - 162
18	X-RAY DIFFRACTION	18	( CHAIN D AND RESID 163:178 )	D	163 - 178
19	X-RAY DIFFRACTION	19	( CHAIN D AND RESID 179:190 )	D	179 - 190
20	X-RAY DIFFRACTION	20	( CHAIN D AND RESID 191:207 )	D	191 - 207
21	X-RAY DIFFRACTION	21	( CHAIN D AND RESID 208:224 )	D	208 - 224
22	X-RAY DIFFRACTION	22	( CHAIN D AND RESID 225:235 )	D	225 - 235
23	X-RAY DIFFRACTION	23	( CHAIN E AND RESID 150:212 )	E	150 - 212
24	X-RAY DIFFRACTION	24	( CHAIN E AND RESID 213:222 )	E	213 - 222
25	X-RAY DIFFRACTION	25	( CHAIN E AND RESID 223:237 )	E	223 - 237
26	X-RAY DIFFRACTION	26	( CHAIN E AND RESID 238:256 )	E	238 - 256
27	X-RAY DIFFRACTION	27	( CHAIN E AND RESID 257:269 )	E	257 - 269
28	X-RAY DIFFRACTION	28	( CHAIN E AND RESID 270:284 )	E	270 - 284
29	X-RAY DIFFRACTION	29	( CHAIN E AND RESID 285:322 )	E	285 - 322
30	X-RAY DIFFRACTION	30	( CHAIN E AND RESID 323:336 )	E	323 - 336
31	X-RAY DIFFRACTION	31	( CHAIN E AND RESID 337:354 )	E	337 - 354
32	X-RAY DIFFRACTION	32	( CHAIN F AND RESID 138:142 )	F	138 - 142
33	X-RAY DIFFRACTION	33	( CHAIN F AND RESID 143:190 )	F	143 - 190
34	X-RAY DIFFRACTION	34	( CHAIN F AND RESID 191:235 )	F	191 - 235
35	X-RAY DIFFRACTION	35	( CHAIN G AND RESID 150:178 )	G	150 - 178
36	X-RAY DIFFRACTION	36	( CHAIN G AND RESID 179:212 )	G	179 - 212
37	X-RAY DIFFRACTION	37	( CHAIN G AND RESID 213:222 )	G	213 - 222
38	X-RAY DIFFRACTION	38	( CHAIN G AND RESID 223:269 )	G	223 - 269
39	X-RAY DIFFRACTION	39	( CHAIN G AND RESID 270:304 )	G	270 - 304
40	X-RAY DIFFRACTION	40	( CHAIN G AND RESID 305:322 )	G	305 - 322
41	X-RAY DIFFRACTION	41	( CHAIN G AND RESID 323:353 )	G	323 - 353
42	X-RAY DIFFRACTION	42	( CHAIN H AND RESID 140:178 )	H	140 - 178
43	X-RAY DIFFRACTION	43	( CHAIN H AND RESID 179:202 )	H	179 - 202
44	X-RAY DIFFRACTION	44	( CHAIN H AND RESID 203:235 )	H	203 - 235
45	X-RAY DIFFRACTION	45	( CHAIN I AND RESID 151:163 )	I	151 - 163
46	X-RAY DIFFRACTION	46	( CHAIN I AND RESID 164:188 )	I	164 - 188
47	X-RAY DIFFRACTION	47	( CHAIN I AND RESID 189:202 )	I	189 - 202
48	X-RAY DIFFRACTION	48	( CHAIN I AND RESID 203:237 )	I	203 - 237
49	X-RAY DIFFRACTION	49	( CHAIN I AND RESID 238:269 )	I	238 - 269
50	X-RAY DIFFRACTION	50	( CHAIN I AND RESID 270:295 )	I	270 - 295
51	X-RAY DIFFRACTION	51	( CHAIN I AND RESID 296:314 )	I	296 - 314
52	X-RAY DIFFRACTION	52	( CHAIN I AND RESID 315:353 )	I	315 - 353
53	X-RAY DIFFRACTION	53	( CHAIN J AND RESID 138:142 )	J	138 - 142
54	X-RAY DIFFRACTION	54	( CHAIN J AND RESID 143:178 )	J	143 - 178
55	X-RAY DIFFRACTION	55	( CHAIN J AND RESID 179:235 )	J	179 - 235
56	X-RAY DIFFRACTION	56	( CHAIN K AND RESID 150:202 )	K	150 - 202
57	X-RAY DIFFRACTION	57	( CHAIN K AND RESID 203:245 )	K	203 - 245
58	X-RAY DIFFRACTION	58	( CHAIN K AND RESID 246:304 )	K	246 - 304
59	X-RAY DIFFRACTION	59	( CHAIN K AND RESID 305:325 )	K	305 - 325
60	X-RAY DIFFRACTION	60	( CHAIN K AND RESID 326:354 )	K	326 - 354
61	X-RAY DIFFRACTION	61	( CHAIN L AND RESID 140:170 )	L	140 - 170
62	X-RAY DIFFRACTION	62	( CHAIN L AND RESID 171:178 )	L	171 - 178
63	X-RAY DIFFRACTION	63	( CHAIN L AND RESID 179:189 )	L	179 - 189
64	X-RAY DIFFRACTION	64	( CHAIN L AND RESID 190:207 )	L	190 - 207
65	X-RAY DIFFRACTION	65	( CHAIN L AND RESID 208:224 )	L	208 - 224
66	X-RAY DIFFRACTION	66	( CHAIN L AND RESID 225:235 )	L	225 - 235
67	X-RAY DIFFRACTION	67	( CHAIN M AND RESID 151:269 )	M	151 - 269
68	X-RAY DIFFRACTION	68	( CHAIN M AND RESID 270:354 )	M	270 - 354
69	X-RAY DIFFRACTION	69	( CHAIN N AND RESID 141:178 )	N	141 - 178
70	X-RAY DIFFRACTION	70	( CHAIN N AND RESID 179:202 )	N	179 - 202
71	X-RAY DIFFRACTION	71	( CHAIN N AND RESID 203:235 )	N	203 - 235
72	X-RAY DIFFRACTION	72	( CHAIN O AND RESID 150:176 )	O	150 - 176
73	X-RAY DIFFRACTION	73	( CHAIN O AND RESID 177:188 )	O	177 - 188
74	X-RAY DIFFRACTION	74	( CHAIN O AND RESID 189:202 )	O	189 - 202
75	X-RAY DIFFRACTION	75	( CHAIN O AND RESID 203:222 )	O	203 - 222
76	X-RAY DIFFRACTION	76	( CHAIN O AND RESID 223:256 )	O	223 - 256
77	X-RAY DIFFRACTION	77	( CHAIN O AND RESID 257:269 )	O	257 - 269
78	X-RAY DIFFRACTION	78	( CHAIN O AND RESID 270:295 )	O	270 - 295
79	X-RAY DIFFRACTION	79	( CHAIN O AND RESID 296:314 )	O	296 - 314
80	X-RAY DIFFRACTION	80	( CHAIN O AND RESID 315:338 )	O	315 - 338
81	X-RAY DIFFRACTION	81	( CHAIN O AND RESID 339:354 )	O	339 - 354
82	X-RAY DIFFRACTION	82	( CHAIN P AND RESID 141:162 )	P	141 - 162
83	X-RAY DIFFRACTION	83	( CHAIN P AND RESID 163:178 )	P	163 - 178
84	X-RAY DIFFRACTION	84	( CHAIN P AND RESID 179:190 )	P	179 - 190
85	X-RAY DIFFRACTION	85	( CHAIN P AND RESID 191:202 )	P	191 - 202
86	X-RAY DIFFRACTION	86	( CHAIN P AND RESID 203:235 )	P	203 - 235
87	X-RAY DIFFRACTION	87	( CHAIN Q AND RESID 151:230 )	Q	151 - 230
88	X-RAY DIFFRACTION	88	( CHAIN Q AND RESID 231:295 )	Q	231 - 295
89	X-RAY DIFFRACTION	89	( CHAIN Q AND RESID 296:314 )	Q	296 - 314
90	X-RAY DIFFRACTION	90	( CHAIN Q AND RESID 315:354 )	Q	315 - 354
91	X-RAY DIFFRACTION	91	( CHAIN R AND RESID 141:170 )	R	141 - 170
92	X-RAY DIFFRACTION	92	( CHAIN R AND RESID 171:190 )	R	171 - 190
93	X-RAY DIFFRACTION	93	( CHAIN R AND RESID 191:202 )	R	191 - 202
94	X-RAY DIFFRACTION	94	( CHAIN R AND RESID 203:235 )	R	203 - 235
95	X-RAY DIFFRACTION	95	( CHAIN S AND RESID 151:178 )	S	151 - 178
96	X-RAY DIFFRACTION	96	( CHAIN S AND RESID 179:202 )	S	179 - 202
97	X-RAY DIFFRACTION	97	( CHAIN S AND RESID 203:237 )	S	203 - 237
98	X-RAY DIFFRACTION	98	( CHAIN S AND RESID 238:256 )	S	238 - 256
99	X-RAY DIFFRACTION	99	( CHAIN S AND RESID 257:269 )	S	257 - 269
100	X-RAY DIFFRACTION	100	( CHAIN S AND RESID 270:295 )	S	270 - 295
101	X-RAY DIFFRACTION	101	( CHAIN S AND RESID 296:311 )	S	296 - 311
102	X-RAY DIFFRACTION	102	( CHAIN S AND RESID 312:321 )	S	312 - 321
103	X-RAY DIFFRACTION	103	( CHAIN S AND RESID 322:336 )	S	322 - 336
104	X-RAY DIFFRACTION	104	( CHAIN S AND RESID 337:353 )	S	337 - 353
105	X-RAY DIFFRACTION	105	( CHAIN T AND RESID 141:178 )	T	141 - 178
106	X-RAY DIFFRACTION	106	( CHAIN T AND RESID 179:190 )	T	179 - 190
107	X-RAY DIFFRACTION	107	( CHAIN T AND RESID 191:202 )	T	191 - 202
108	X-RAY DIFFRACTION	108	( CHAIN T AND RESID 203:235 )	T	203 - 235