+Open data
-Basic information
Entry | Database: PDB / ID: 8j73 | ||||||
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Title | Methylcrotonoyl-CoA carboxylase core-Dimer | ||||||
Components | Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial | ||||||
Keywords | CYTOSOLIC PROTEIN / MCC / Mitochondria | ||||||
Function / homology | Function and homology information 3-methylcrotonyl-CoA carboxylase complex, mitochondrial / methylcrotonoyl-CoA carboxylase / methylcrotonoyl-CoA carboxylase activity / methylcrotonoyl-CoA carboxylase complex / Defective HLCS causes multiple carboxylase deficiency / Biotin transport and metabolism / L-leucine catabolic process / branched-chain amino acid catabolic process / Branched-chain amino acid catabolism / coenzyme A metabolic process ...3-methylcrotonyl-CoA carboxylase complex, mitochondrial / methylcrotonoyl-CoA carboxylase / methylcrotonoyl-CoA carboxylase activity / methylcrotonoyl-CoA carboxylase complex / Defective HLCS causes multiple carboxylase deficiency / Biotin transport and metabolism / L-leucine catabolic process / branched-chain amino acid catabolic process / Branched-chain amino acid catabolism / coenzyme A metabolic process / mitochondrial matrix / mitochondrion / ATP binding / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.16 Å | ||||||
Authors | Liu, D.S. / Su, J.Y. | ||||||
Funding support | China, 1items
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Citation | Journal: To Be Published Title: Methylcrotonoyl-CoA carboxylase core-Dimer Authors: Liu, D.S. / Su, J.Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8j73.cif.gz | 1.1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb8j73.ent.gz | 981.8 KB | Display | PDB format |
PDBx/mmJSON format | 8j73.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/j7/8j73 ftp://data.pdbj.org/pub/pdb/validation_reports/j7/8j73 | HTTPS FTP |
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-Related structure data
Related structure data | 36024MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 61406.027 Da / Num. of mol.: 12 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: MCCC2, MCCB / Production host: Homo sapiens (human) References: UniProt: Q9HCC0, methylcrotonoyl-CoA carboxylase |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Methylcrotonoyl-CoA carboxylase core-Dimer / Type: COMPLEX / Entity ID: all / Source: NATURAL |
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Source (natural) | Organism: Homo sapiens (human) |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 1800 nm / Nominal defocus min: 1300 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING ONLY |
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3D reconstruction | Resolution: 4.16 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 1474 / Symmetry type: POINT |