+Open data
-Basic information
Entry | Database: PDB / ID: 8gbe | ||||||
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Title | Structure of a viral gasdermin protein A47 from Eptesipox virus | ||||||
Components | Protein A47 | ||||||
Keywords | VIRAL PROTEIN / viral mimicry / gasdermin / caspase / autoinhibition / pyroptosis / bats / immunity / cell death / immune system | ||||||
Function / homology | Gasdermin / Orthopoxvirus A47 / Orthopoxvirus A47 protein / Gasdermin, PUB domain / Gasdermin PUB domain / Protein A47 Function and homology information | ||||||
Biological species | Eptesipox virus | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 1.46 Å | ||||||
Authors | Johnson, A.G. / Kranzusch, P.J. | ||||||
Funding support | United States, 1items
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Citation | Journal: Biorxiv / Year: 2023 Title: Structural homology screens reveal poxvirus-encoded proteins impacting inflammasome-mediated defenses. Authors: Boys, I.N. / Johnson, A.G. / Quinlan, M. / Kranzusch, P.J. / Elde, N.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8gbe.cif.gz | 115 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8gbe.ent.gz | 74.2 KB | Display | PDB format |
PDBx/mmJSON format | 8gbe.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gb/8gbe ftp://data.pdbj.org/pub/pdb/validation_reports/gb/8gbe | HTTPS FTP |
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-Related structure data
Similar structure data | Similarity search - Function & homologyF&H Search |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 23802.727 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Eptesipox virus / Gene: EPTV-WA-146 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): RIL / References: UniProt: A0A220T6L2 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48.74 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6.4 / Details: 100 mM ADA and 40% PEG-200 |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.97911 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Dec 9, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97911 Å / Relative weight: 1 |
Reflection | Resolution: 1.46→48.65 Å / Num. obs: 40114 / % possible obs: 100 % / Redundancy: 13.5 % / Biso Wilson estimate: 21.91 Å2 / CC1/2: 0.999 / Rpim(I) all: 0.019 / Net I/σ(I): 18.3 |
Reflection shell | Resolution: 1.46→1.48 Å / Redundancy: 13.2 % / Mean I/σ(I) obs: 1.6 / Num. unique obs: 1969 / CC1/2: 0.505 / Rpim(I) all: 0.718 / % possible all: 99.6 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 1.46→48.65 Å / SU ML: 0.1496 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 21.0281 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 33.31 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.46→48.65 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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