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Open data
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Basic information
Entry | Database: PDB / ID: 7zct | ||||||
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Title | Structure of the red fluorescent protein mScarlet3 at pH 7.5 | ||||||
![]() | Red fluorescent protein drFP583 | ||||||
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Function / homology | Green fluorescent protein, GFP / Green fluorescent protein-related / ![]() ![]() ![]() ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Aumonier, S. / Dupuy, J. / Royant, A. | ||||||
Funding support | ![]()
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![]() | ![]() Title: mScarlet3: a brilliant and fast-maturing red fluorescent protein. Authors: Gadella Jr., T.W.J. / van Weeren, L. / Stouthamer, J. / Hink, M.A. / Wolters, A.H.G. / Giepmans, B.N.G. / Aumonier, S. / Dupuy, J. / Royant, A. #1: ![]() Title: mScarlet: a bright monomeric red fluorescent protein for cellular imaging. Authors: Bindels, D.S. / Haarbosch, L. / van Weeren, L. / Postma, M. / Wiese, K.E. / Mastop, M. / Aumonier, S. / Gotthard, G. / Royant, A. / Hink, M.A. / Gadella, T.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 109.1 KB | Display | ![]() |
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PDB format | ![]() | 82.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5lk4S S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 25865.129 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: Residue NRQ67 is formed by the autocatalytic cyclisation of the three consecutive amino acid residues M67, Y68 and G69 Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | ![]() Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.77 Å3/Da / Density % sol: 30.42 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 0.1 M sodium chloride, 0.1 M, 0.1 M HEPES pH 7.5, 1.6 M Ammonium sulfate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Sep 10, 2020 |
Radiation | Monochromator: CHANNEL-CUT SI(111) CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.33→42.78 Å / Num. obs: 91873 / % possible obs: 97.5 % / Redundancy: 3.8 % / Biso Wilson estimate: 16.7 Å2 / CC1/2: 0.994 / Rsym value: 0.097 / Net I/σ(I): 6 |
Reflection shell | Resolution: 1.33→1.38 Å / Mean I/σ(I) obs: 0.7 / Num. unique obs: 9289 / CC1/2: 0.548 / Rsym value: 0.929 / % possible all: 99.1 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 5LK4 Resolution: 1.33→42.78 Å / Cor.coef. Fo:Fc: 0.969 / Cor.coef. Fo:Fc free: 0.955 / SU B: 1.84 / SU ML: 0.07 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.061 / ESU R Free: 0.063 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 67.36 Å2 / Biso mean: 18.863 Å2 / Biso min: 12.02 Å2
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Refinement step | Cycle: final / Resolution: 1.33→42.78 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.33→1.362 Å / Rfactor Rfree error: 0
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