デオキシリボ核酸 / DNA (> 10) / DNA (> 100) / リボ核酸 / RNA (> 10) / FACT complex subunit / Transcription elongation factor 1 homolog / DNA-directed RNA polymerase subunit beta / RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III / Transcription elongation factor SPT4 ...デオキシリボ核酸 / DNA (> 10) / DNA (> 100) / リボ核酸 / RNA (> 10) / FACT complex subunit / Transcription elongation factor 1 homolog / DNA-directed RNA polymerase subunit beta / RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III / Transcription elongation factor SPT4 / Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p / RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / RNA polymerase II subunit B32 / Transcription elongation factor SPT5 / RNA polymerase-associated protein LEO1 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / RNA polymerase II subunit B12.5 / DNA-directed RNA polymerase subunit / Component of the Paf1p complex / Transcription elongation factor Spt6 / RNA polymerase II third largest subunit B44, part of central core / Protein that interacts with Spt6p and copurifies with Spt5p and RNA polymerase II / RNAP II-associated protein / DNA-directed RNA polymerase subunit / RNA polymerase subunit ABC10-alpha / FACT complex subunit POB3 / DNA-directed RNA polymerase subunit / RNAPII-associated chromatin remodeling Paf1 complex subunit / Histone H2A type 1-B/E / Histone H2B type 1-J / ヒストンH4 / Histone H3.3 類似検索 - 構成要素
生物種
Komagataella phaffii (菌類) Homo sapiens (ヒト) synthetic construct (人工物)
Japan Agency for Medical Research and Development (AMED)
JP20am0101082
日本
Japan Agency for Medical Research and Development (AMED)
JP21am0101076
日本
Japan Science and Technology
JPMJER1901
日本
引用
ジャーナル: Science / 年: 2022 タイトル: Structural basis of nucleosome disassembly and reassembly by RNAPII elongation complex with FACT. 著者: Haruhiko Ehara / Tomoya Kujirai / Mikako Shirouzu / Hitoshi Kurumizaka / Shun-Ichi Sekine / 要旨: During gene transcription, RNA polymerase II (RNAPII) traverses nucleosomes in chromatin, but the mechanism has remained elusive. Using cryo-electron microscopy, we obtained structures of the RNAPII ...During gene transcription, RNA polymerase II (RNAPII) traverses nucleosomes in chromatin, but the mechanism has remained elusive. Using cryo-electron microscopy, we obtained structures of the RNAPII elongation complex (EC) passing through a nucleosome in the presence of the transcription elongation factors Spt6, Spn1, Elf1, Spt4/5, and Paf1C and the histone chaperone FACT (facilitates chromatin transcription). The structures show snapshots of EC progression on DNA mediating downstream nucleosome disassembly, followed by its reassembly upstream of the EC, which is facilitated by FACT. FACT dynamically adapts to successively occurring subnucleosome intermediates, forming an interface with the EC. Spt6, Spt4/5, and Paf1C form a "cradle" at the EC DNA-exit site and support the upstream nucleosome reassembly. These structures explain the mechanism by which the EC traverses nucleosomes while maintaining the chromatin structure and epigenetic information.
A: DNA-directed RNA polymerase subunit B: DNA-directed RNA polymerase subunit beta C: RNA polymerase II third largest subunit B44, part of central core D: RNA polymerase II subunit B32 E: DNA-directed RNA polymerases I, II, and III subunit RPABC1 F: RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III G: RNA polymerase II subunit H: DNA-directed RNA polymerases I, II, and III subunit RPABC3 I: DNA-directed RNA polymerase subunit J: RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III K: RNA polymerase II subunit B12.5 L: RNA polymerase subunit ABC10-alpha M: Transcription elongation factor 1 homolog N: DNA (198-MER) P: RNA (5'-R(P*GP*AP*CP*CP*CP*GP*GP*GP*UP*GP*UP*CP*UP*UP*CP*CP*CP*CP*A)-3') T: DNA (198-MER) V: Transcription elongation factor SPT4 W: Transcription elongation factor SPT5 m: Transcription elongation factor Spt6 n: Protein that interacts with Spt6p and copurifies with Spt5p and RNA polymerase II q: Component of the Paf1p complex r: RNAPII-associated chromatin remodeling Paf1 complex subunit u: Leo1 v: RNAP II-associated protein x: Constituent of Paf1 complex with RNA polymerase II, Paf1p, Hpr1p, Ctr9, Leo1, Rtf1 and Ccr4p a: Histone H3.3 b: Histone H4 c: Histone H2A type 1-B/E d: Histone H2B type 1-J e: Histone H3.3 f: Histone H4 g: Histone H2A type 1-B/E h: Histone H2B type 1-J j: FACT complex subunit k: FACT complex subunit POB3 ヘテロ分子