+Open data
-Basic information
Entry | Database: PDB / ID: 7w24 | ||||||
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Title | Structure of the M. tuberculosis HtrA N383A mutant | ||||||
Components | Probable serine protease HtrA1 | ||||||
Keywords | HYDROLASE / HtrA family of serine protease / chymotrypsin-like / Periplasm / Protein quality control and signal transduction. | ||||||
Function / homology | Function and homology information peptidase Do / peptidoglycan-based cell wall / serine-type endopeptidase activity / proteolysis / plasma membrane Similarity search - Function | ||||||
Biological species | Mycobacterium tuberculosis H37Rv (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.9 Å | ||||||
Authors | Gupta, A.K. / Gopal, B. | ||||||
Funding support | India, 1items
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Citation | Journal: Acs Chem.Biol. / Year: 2023 Title: Allosteric Determinants in High Temperature Requirement A Enzymes Are Conserved and Regulate the Population of Active Conformations. Authors: Gupta, A.K. / Singh, K. / Patidar, Y. / Sharma, R. / Sardesai, A.A. / Reddy, G. / Gopal, B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7w24.cif.gz | 63.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7w24.ent.gz | 41.8 KB | Display | PDB format |
PDBx/mmJSON format | 7w24.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/w2/7w24 ftp://data.pdbj.org/pub/pdb/validation_reports/w2/7w24 | HTTPS FTP |
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-Related structure data
Related structure data | 7vyzC 7vz0C 7w21C 7w22C 7w23C 7w25C 7w4rC 7w4sC 7w4tC 7w4uC 7w4vC 7w4wC 6ieoS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 31431.215 Da / Num. of mol.: 1 / Mutation: N383A Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mycobacterium tuberculosis H37Rv (bacteria) Strain: ATCC 25618 / H37Rv / Gene: htrA1, degP, htrA, Rv1223 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: O06291, peptidase Do |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 39.28 % |
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Crystal grow | Temperature: 298 K / Method: microbatch / pH: 7.4 Details: 0.1M HEPES, 1.5M Sodium Citrate tribasic dihydrate pH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ROTATING ANODE / Type: BRUKER AXS MICROSTAR / Wavelength: 1.5418 Å |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Jul 15, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→30.16 Å / Num. obs: 5544 / % possible obs: 97.9 % / Redundancy: 3.4 % / Biso Wilson estimate: 39.61 Å2 / CC1/2: 0.98 / Net I/σ(I): 6.7 |
Reflection shell | Resolution: 2.901→3.004 Å / Num. unique obs: 559 / CC1/2: 0.91 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6IEO Resolution: 2.9→30.16 Å / Cross valid method: FREE R-VALUE / σ(F): 28.4 / Phase error: 38.35 / Stereochemistry target values: TWIN_LSQ_F
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.9→30.16 Å
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Refine LS restraints |
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LS refinement shell |
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