PDB-7st3: Consequences of HLA single chain trimer mutations on peptide pres...

Basic information

• Entry: Database: PDB / ID: 7st3
• Title: Consequences of HLA single chain trimer mutations on peptide presentation and binding affinity

Components

• Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera
• VHH

• Keywords: IMMUNE SYSTEM/VIRAL PROTEIN / HLA / VHH / SCT / HPV / IMMUNE SYSTEM-VIRAL PROTEIN complex

Function / homology

Function:

symbiont-mediated suppression of host JAK-STAT cascade via inhibition of host IRF9 activity / symbiont-mediated activation of of host transcription / : / : / antigen processing and presentation of peptide antigen via MHC class I / positive regulation of actin filament polymerization / cadmium ion binding / symbiont-mediated perturbation of host cell cycle G1/S transition checkpoint / antigen processing and presentation / viral process / lumenal side of endoplasmic reticulum membrane / ER to Golgi transport vesicle membrane / MHC class I protein complex / recycling endosome membrane / phagocytic vesicle membrane / early endosome membrane / DNA-binding transcription factor binding / host cell cytoplasm / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / immune response / DNA-binding transcription factor activity / protein domain specific binding / DNA-templated transcription / host cell nucleus / cell surface / DNA binding / zinc ion binding / extracellular region

Domain/homology:

Papillomavirus E7 / E7 protein, Early protein / MHC class I, alpha chain, C-terminal / MHC_I C-terminus / MHC class I alpha chain, alpha1 alpha2 domains / Class I Histocompatibility antigen, domains alpha 1 and 2 / Beta-2-Microglobulin / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold

Component:

MHC class I antigen / Protein E7 / Beta-2-microglobulin

• Biological species: Human papillomavirus type 16; Pongo pygmaeus (Bornean orangutan); Homo sapiens (human); Lama glama (llama)
• Method: X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.78 Å
• Authors: Finton, K.A.K. / Rupert, P.B.

Funding support

United States, 1items

Organization	Grant number	Country
National Institutes of Health/National Cancer Institute (NIH/NCI)		United States

• Citation: Journal: Front Immunol / Year: 2023; Title: Effects of HLA single chain trimer design on peptide presentation and stability.; Authors: Finton, K.A.K. / Rupert, P.B. / Friend, D.J. / Dinca, A. / Lovelace, E.S. / Buerger, M. / Rusnac, D.V. / Foote-McNabb, U. / Chour, W. / Heath, J.R. / Campbell, J.S. / Pierce, R.H. / Strong, R.K.

History

Deposition	Nov 11, 2021	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Nov 23, 2022	Provider: repository / Type: Initial release
Revision 1.1	May 31, 2023	Group: Database references / Category: citation / citation_author Item: _citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2	Oct 25, 2023	Group: Data collection / Refinement description Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

Assembly

Deposited unit

A: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

B: VHH

C: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

D: VHH

E: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

F: VHH

G: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

H: VHH

I: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

J: VHH

K: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

L: VHH

M: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

N: VHH

O: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

P: VHH

Q: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

R: VHH

S: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

T: VHH

U: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

V: VHH

W: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

X: VHH

Y: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

Z: VHH

a: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

b: VHH

c: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

d: VHH

e: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

f: VHH

Summary:

• 973 kDa, 32 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	973,054	32
Polymers	973,054	32
Non-polymers	0	0
Water	3,567	198

1

A: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

B: VHH

Summary:

• defined by author
• Evidence: surface plasmon resonance
• 60.8 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	60,816	2
Polymers	60,816	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

2

C: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

D: VHH

Summary:

• defined by author
• 60.8 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	60,816	2
Polymers	60,816	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

3

E: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

F: VHH

Summary:

• defined by author
• 60.8 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	60,816	2
Polymers	60,816	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

4

G: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

H: VHH

Summary:

• defined by author
• 60.8 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	60,816	2
Polymers	60,816	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

5

I: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

J: VHH

Summary:

• defined by author
• 60.8 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	60,816	2
Polymers	60,816	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

6

K: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

L: VHH

Summary:

• defined by author
• 60.8 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	60,816	2
Polymers	60,816	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

7

M: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

N: VHH

Summary:

• defined by author
• 60.8 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	60,816	2
Polymers	60,816	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

8

O: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

P: VHH

Summary:

• defined by author
• 60.8 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	60,816	2
Polymers	60,816	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

9

Q: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

R: VHH

Summary:

• defined by author
• 60.8 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	60,816	2
Polymers	60,816	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

10

S: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

T: VHH

Summary:

• defined by author
• 60.8 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	60,816	2
Polymers	60,816	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

11

U: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

V: VHH

Summary:

• defined by author
• 60.8 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	60,816	2
Polymers	60,816	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

12

W: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

X: VHH

Summary:

• defined by author
• 60.8 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	60,816	2
Polymers	60,816	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

13

Y: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

Z: VHH

Summary:

• defined by author
• 60.8 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	60,816	2
Polymers	60,816	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

14

a: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

b: VHH

Summary:

• defined by author
• 60.8 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	60,816	2
Polymers	60,816	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

15

c: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

d: VHH

Summary:

• defined by author
• 60.8 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	60,816	2
Polymers	60,816	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

16

e: Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

f: VHH

Summary:

• defined by author
• 60.8 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	60,816	2
Polymers	60,816	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Unit cell

Length a, b, c (Å)	117.796, 118.041, 273.507
Angle α, β, γ (deg.)	102.449, 102.452, 89.998
Int Tables number	1
Space group name H-M	P1
Space group name Hall	P1
Symmetry operation	#1: x,y,z

Components

#1: Protein

A C E G I K M O Q S U W Y a c e
Protein E7 peptide,Beta-2-microglobulin,MHC class I antigen chimera

Details:

Mass: 48279.074 Da / Num. of mol.: 16 / Mutation: Y108C,A163C
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human papillomavirus type 16, (gene. exp.) Pongo pygmaeus (Bornean orangutan), (gene. exp.) Homo sapiens (human)
Gene: E7, B2M, HLA-A / Production host: Homo sapiens (human)
References: UniProt: P03129, UniProt: P16213, UniProt: A0A678ZGP6

#2: Antibody

B D F H J L N P R T V X Z b d f
VHH

Details:

Mass: 12536.822 Da / Num. of mol.: 16
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Lama glama (llama) / Production host: Homo sapiens (human)

#3: Water

ChemComp-HOH / water / Water

Details:

Mass: 18.015 Da / Num. of mol.: 198 / Source method: isolated from a natural source / Formula: H₂O

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 3.72 ų/Da / Density % sol: 66.96 %
• Crystal grow: Temperature: 295 K / Method: vapor diffusion, hanging drop / Details: 100 mM Tris, pH 7.5, 200 mM Li2SO4, 1.6 M AmSO4

Data collection

• Diffraction: Mean temperature: 93 K / Serial crystal experiment: N
• Diffraction source: Source: SYNCHROTRON / Site: ALS / Beamline: 5.0.1 / Wavelength: 1 Å
• Detector: Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Aug 13, 2021
• Radiation: Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 1 Å / Relative weight: 1
• Reflection: Resolution: 2.78→50.48 Å / Num. obs: 340982 / % possible obs: 98.2 % / Redundancy: 1.8 % / B_iso Wilson estimate: 47.42 Ų / CC_1/2: 0.998 / Net I/σ(I): 9.76
• Reflection shell: Resolution: 2.78→2.88 Å / Num. unique obs: 29320 / CC_1/2: 0.916

Processing

• Software: Name: PHENIX / Version: 1.19.1_4122 / Classification: refinement

Refinement

Method to determine structure: MOLECULAR REPLACEMENT
Starting model: 7SR5

7sr5

Resolution: 2.78→50.48 Å / SU ML: 0.3673 / Cross valid method: FREE R-VALUE / σ(F): 1.98 / Phase error: 26.8582
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	Rfactor	Num. reflection	% reflection
Rfree	0.2747	17167	5.04 %
Rwork	0.2376	323689	-
obs	0.2395	340856	96.75 %

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
• Displacement parameters: B_iso mean: 55.89 Ų

Refinement step

Cycle: LAST / Resolution: 2.78→50.48 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	57639	0	0	198	57837

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.0032	59107
X-RAY DIFFRACTION	f_angle_d	0.5767	80530
X-RAY DIFFRACTION	f_chiral_restr	0.041	8648
X-RAY DIFFRACTION	f_plane_restr	0.0045	10465
X-RAY DIFFRACTION	f_dihedral_angle_d	5.1512	8342

LS refinement shell

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	Refine-ID	% reflection obs (%)
2.78-2.81	0.3908	344	0.3496	6186	X-RAY DIFFRACTION	55.81
2.81-2.85	0.3736	488	0.3114	10697	X-RAY DIFFRACTION	96.32
2.85-2.88	0.3376	694	0.3005	10911	X-RAY DIFFRACTION	97.3
2.88-2.92	0.3252	631	0.2949	10706	X-RAY DIFFRACTION	97.57
2.92-2.96	0.3214	689	0.2808	10873	X-RAY DIFFRACTION	97.92
2.96-3	0.308	625	0.2779	10960	X-RAY DIFFRACTION	98.26
3-3.04	0.3033	564	0.2718	10972	X-RAY DIFFRACTION	98.03
3.04-3.08	0.3038	678	0.2698	10791	X-RAY DIFFRACTION	97.32
3.08-3.13	0.2882	556	0.2636	10863	X-RAY DIFFRACTION	97.76
3.13-3.18	0.3235	559	0.2753	10945	X-RAY DIFFRACTION	97.54
3.18-3.24	0.3075	569	0.2781	10676	X-RAY DIFFRACTION	96.81
3.24-3.3	0.2928	533	0.2453	11030	X-RAY DIFFRACTION	97.72
3.3-3.36	0.3139	487	0.2439	10970	X-RAY DIFFRACTION	98.2
3.36-3.43	0.2856	601	0.242	10974	X-RAY DIFFRACTION	98.29
3.43-3.5	0.2815	582	0.2381	10903	X-RAY DIFFRACTION	98.19
3.5-3.59	0.2602	535	0.2294	11027	X-RAY DIFFRACTION	97.77
3.59-3.68	0.2953	472	0.2383	11035	X-RAY DIFFRACTION	98.07
3.68-3.78	0.2694	642	0.2331	10920	X-RAY DIFFRACTION	98.56
3.78-3.89	0.2522	594	0.2209	10988	X-RAY DIFFRACTION	98.5
3.89-4.01	0.2698	483	0.2242	11048	X-RAY DIFFRACTION	98.3
4.01-4.15	0.2357	633	0.2123	10897	X-RAY DIFFRACTION	98.01
4.15-4.32	0.2406	619	0.2119	11021	X-RAY DIFFRACTION	99
4.32-4.52	0.2377	560	0.2063	11108	X-RAY DIFFRACTION	99.04
4.52-4.76	0.2368	622	0.2042	10957	X-RAY DIFFRACTION	99.16
4.76-5.05	0.2447	624	0.2107	11010	X-RAY DIFFRACTION	99.22
5.05-5.44	0.2612	578	0.2195	10962	X-RAY DIFFRACTION	98.41
5.44-5.99	0.2907	514	0.2329	11104	X-RAY DIFFRACTION	98.48
5.99-6.85	0.2645	593	0.2507	11102	X-RAY DIFFRACTION	99.46
6.86-8.63	0.2556	520	0.2273	11075	X-RAY DIFFRACTION	98.78
8.63-50.48	0.2866	578	0.2485	10978	X-RAY DIFFRACTION	98.42

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.957838268286	-0.00806757523101	0.00311421633831	2.24658124182	-0.78664796823	0.251799183311	0.110987269693	-0.0715350384136	-0.00440983695444	0.333295532347	-0.476250726726	-0.764972521329	-0.0654256633902	-0.0052121672207	0.21156095241	0.457803345179	-0.221631084848	-0.0668475068648	0.442496298214	0.112932525938	0.43103188823	-84.9272218506	-61.3088948667	-120.153195729
2	1.72747403062	0.188363531139	-0.527470285217	2.72698921542	0.230550124556	2.21581867865	0.202487683776	-0.18272439167	-0.158010470344	0.409054866467	-0.330584245227	-0.303123752903	-0.198097578869	-0.0964965792647	0.0912959920812	0.300243438774	-0.0306377904798	-0.0729819790541	0.268225660953	0.0739007906156	0.269794470874	-90.2550339281	-67.5349679782	-117.957808002
3	1.59690715479	0.72957466252	-0.0651961985067	3.85916180217	0.709796886863	2.60842245815	-0.103248848692	-0.118355420768	-0.578087106047	-0.21760980083	-0.0260100841733	-0.225502103048	0.0475554047994	-0.208709461197	0.16366216574	0.264534677502	0.0021672426217	0.0508276015477	0.400541847545	0.0377957731163	0.280357650172	-85.9381117245	-58.5347053079	-135.618929093
4	1.99968684388	1.34484777665	-0.152329152566	2.52529255802	0.760315948172	1.45227993106	0.0254758350658	-0.46802644319	-0.366188066433	-0.160341969004	-0.279671702949	-0.0122714518333	0.00541479646808	-0.268467712896	0.162109329045	0.239527401942	0.00689482903767	-0.0531832611043	0.393777307258	0.0337711300653	0.257156748821	-89.794858191	-52.1284840104	-131.560825496
5	1.94272013536	0.263250775251	0.0642694678436	2.96947233994	0.60787151428	2.23275082514	-0.232577914543	0.160024426924	-0.101025006899	-0.538873879299	0.0482424648661	0.197690227164	-0.0152175638367	-0.50854944047	0.211552736881	0.360561282551	-0.0669524534846	0.0522937314617	0.342699833042	-0.00606547187761	0.217631970712	-87.957859703	-52.7300781228	-139.875645902
6	0.425730594182	-0.268660278999	-0.506286697463	2.16055357535	0.807568021746	1.35540199342	-0.330614489173	0.279561458477	-0.540538469468	-0.431153288747	0.404999990197	-0.743380506323	0.114635337745	0.151444916165	-0.0806050955717	0.438402739989	-0.0198644444347	0.168498161364	0.356492469134	0.0208599887172	0.570173157769	-74.7488465096	-58.9025585806	-139.150323393
7	1.25284588225	-0.176815780749	-0.281765330916	2.6055935992	1.26496549206	1.8695303926	0.0195449521293	0.211003377125	-0.312853272156	-0.476867656259	0.0441230157766	-0.452312299387	0.0543343735829	-0.0916800393156	-0.105451127843	0.462487949848	-0.0342651836551	0.136714064352	0.318237801566	0.00440075692757	0.478727001757	-73.273948285	-51.9527748871	-143.252434449
8	0.0204205609146	-0.0622421476086	-0.0232435836324	0.179905120123	0.0129524782542	0.0040611013526	0.493842512	-0.453470874238	-0.392953877832	0.860806212272	-0.466563836836	-0.652721993311	0.383615409574	-0.0932874166112	0.125536450902	0.959252492058	-0.84667016416	-0.465069633568	0.153991207655	0.672396543857	0.829510364014	-73.981615135	-55.5540350429	-109.226595004
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83	2.74749028497	0.630188223094	-0.369293024569	1.9306834814	-0.385450627877	1.90571760151	-0.129675645902	-0.129056383329	0.316658456429	-0.0719643819665	-0.106240883615	0.0114417322066	-0.282244331417	0.19536273665	0.110900844167	0.250292114146	-0.0517716515449	-0.00202370927597	0.268161519699	-0.0216505058315	0.307185617278	-173.820599608	-24.7652794275	-59.4004552415
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88	4.28633959274	-0.979808849099	-1.17773274263	4.43993680971	3.48553359337	4.99412370998	-0.0737320778746	0.453724633724	0.453010636911	-0.445206168141	0.259332417278	0.255953788285	-0.488577370282	-0.158555874285	-0.142347116014	0.234986731438	-0.0909531022031	-0.00151000402772	0.292522165373	0.0455059944783	0.461243779791	-146.154881998	-0.293493387884	-78.8387787067
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91	1.09642247323	-0.460409739241	0.137305962992	0.919483472397	0.24384213593	0.11246132266	-0.411738836072	0.587560625041	0.343523384789	0.437024352133	0.421619099326	-0.081861664373	0.261963060251	0.275072842559	0.0111481444116	0.743072549782	-0.362075475915	-0.24898570459	0.67634226442	0.0777868045458	0.483487087351	-126.853271988	-19.5134576387	-206.529924972
92	2.03301557344	-0.105905309539	-0.252667516784	0.745647967273	0.444776545868	1.65661279964	-0.614332841833	0.60309707304	0.415359835549	0.0951850059897	0.329740671575	-0.0295916931581	0.124416910825	0.213546301092	0.232796070306	0.769760711769	-0.243054774515	-0.176133886194	0.475998420786	0.0835405697021	0.409313786016	-131.340759314	-23.7318860926	-205.656160913
93	4.31164178236	-0.49569777667	-1.53337315865	1.52850769865	-0.424919827479	2.71240179883	-1.22680095149	-0.0450549397056	-0.163515906259	0.260420760665	0.382001406263	-0.0211019240786	0.225339596613	0.0364750472327	0.539793727847	0.923752946175	-0.0726417789267	-0.183550681289	0.337704085328	0.0457127343644	0.36742406108	-118.988976706	-23.69702216	-189.268014429
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95	3.00644900468	-0.403227935258	-2.03158695775	1.08600805484	0.62500138035	2.20210766795	-0.418589975946	-0.354225319949	0.33671372847	0.0876414350319	0.210161459628	0.260619281293	0.16661534194	-0.0926812477172	0.170391467273	0.515780944853	0.0327120311228	-0.212671098457	0.529341857129	-0.0302566620171	0.587071095299	-116.932629416	-8.17289053219	-183.494116727
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97	6.13039955672	-0.809855231063	0.921082970961	7.64382848227	2.04089407323	1.27213953409	0.277827805054	0.234813405178	0.556446028348	0.0838779031078	-0.103789496532	0.158215567172	-0.694215094965	0.134515242004	-0.189180924055	0.964131277521	-0.28178724964	-0.160361787901	0.373488998042	0.182018659372	0.86667795507	-156.064632231	-38.9230137936	-212.79811941
98	6.91655671054	-2.56851220602	3.39055236888	1.28094510676	-1.2536325646	1.66048197739	0.00775641501917	0.0773403378177	-0.270677821269	-0.322075838642	-0.0998592493294	0.340971235838	0.0684241944647	-0.166893495754	-0.00439070933012	0.646485775526	-0.0445550295837	-0.0129534225498	0.630425106966	0.163457080895	0.455798649737	-156.183358709	-47.9276809081	-192.002366328
99	5.626108428	1.44862531385	0.592834464275	7.36502564015	-1.08955071749	2.09232115367	-0.547646653513	1.05128022269	-0.15287496271	0.00417423506698	0.771454132386	0.543078400139	-0.747233519152	0.317109249065	-0.0295827770082	0.539608201146	-0.179379800303	-0.012763219332	0.488944982521	-0.0207268988725	0.468155907594	-149.552575769	-41.0706831881	-208.940351142
100	2.80817392245	-0.00995681984979	0.586030488687	2.8071831887	-1.58654879206	2.43067261437	-0.407882558237	0.0335607095624	-0.301631887362	0.29444205398	0.404104320386	-0.125661671341	-0.339229350345	0.02180663849	-0.0804772807912	0.559354709587	-0.0485686125691	0.0490245344102	0.397720354016	0.073916304219	0.530462003079	-142.085757801	-38.7278551342	-202.301669224
101	4.18727952038	0.379054036486	0.695041148745	3.49950838637	-1.62496149295	1.82288320582	-0.430717030611	0.33363260381	-0.171058504345	-0.573141284876	0.454729555059	-0.173269244667	0.101964422809	0.198619864884	0.101041350043	0.632749678839	-0.0234406557178	0.100368214991	0.33140977556	0.0294062736332	0.522261343279	-146.942715817	-48.1054002583	-206.70592269
102	2.94836159962	0.250619435278	-0.999305842425	1.57028029599	-0.597367526758	3.67887054869	-0.41572635539	0.479908809029	-0.152331285955	0.0819959884558	0.640193331698	0.152362099267	-0.439844115775	0.326008176216	-0.361593574543	0.682870931912	-0.0941538270296	0.0394841292897	0.470774483408	0.0507880120282	0.41111568065	-150.49447031	-37.3369645525	-203.289174985
103	2.10394725779	-0.207216661033	0.756931585746	0.707237905586	-0.176698258178	0.286259531979	-0.798484243173	0.531089443675	0.244818194307	0.0462095404998	0.210576128315	0.0860248393385	-0.208254451461	0.264476095326	0.490919280507	0.811216022903	-0.284008431173	-0.0835470511574	0.591910424442	-0.0387346455837	0.623895607069	-70.0224166119	-77.6638647929	-205.888937129
104	0.507498394612	-0.695322213345	-0.503015688311	1.49530902239	0.740008976681	0.556117431872	-1.04184163524	0.649328767106	0.197198509941	0.147846662266	0.588223304013	-0.160709121161	0.070090510706	0.0794947279315	0.124988213097	1.07415161436	-0.435685151233	-0.263515648661	0.754234297197	-0.0425709728699	0.377321979351	-73.8855747886	-83.4421606219	-207.547945745
105	2.19202375523	0.130773924505	0.106362453619	0.473305396387	0.103027639397	0.954450209403	-0.832475264015	-0.165311224334	0.157548015349	-0.200820907391	0.36204555944	-0.0800835739384	0.242208674831	-0.146176659695	0.337545066311	0.887717038333	-0.0617030947771	-0.156805027143	0.395488739098	0.00843648331122	0.452310759406	-66.6229724896	-82.0269349387	-193.977199186
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107	2.64337835278	-0.215456992093	-0.172550400375	1.50692336247	0.0873882768234	1.98012681189	-0.423760479263	-0.574340913209	0.404844480132	-0.0531227314121	-0.0847855261579	0.389849948619	0.0135199766726	-0.336015912678	0.39248286931	0.524422119421	0.00378956865218	-0.164046169558	0.518173573081	-0.19082336171	0.668559890104	-60.4597778136	-68.1415574022	-182.100128098
108	0.0411490251795	0.0970242224403	-0.0807593636387	0.302648931479	-0.18768286424	0.232521278422	-0.404225451065	0.583304284847	0.507078783966	0.212636358417	0.294176643538	0.270583733582	-0.501034627953	0.291564523956	0.0323582109893	1.17137923659	-0.434986031541	-0.32770091756	0.677379462867	0.23325381773	0.939013125906	-67.2707027938	-64.7827629742	-214.109787492
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110	2.7811009502	-1.26693340755	1.38094625082	8.09054941937	-5.7708027129	4.19553627097	-0.416696792882	0.416288312588	0.144896958613	-0.221628701944	0.0921215408411	-0.038002129781	-0.213663892555	0.569701761469	0.302780873491	0.542327470264	0.00441229441776	-0.095652854212	0.701388131172	-0.00111623665181	0.494322371909	-96.3449268014	-103.973649372	-210.310717338
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113	3.22233915483	0.566479341701	-0.367062364303	2.8436878961	0.128449658265	0.0564821753265	-0.60156417668	0.524141685516	-0.189887711984	-0.37363559618	0.333491905772	-0.197617774908	0.0316268483767	0.458486258087	0.219944323422	0.681309523256	-0.0271008468922	0.0778294828382	0.398539136873	0.0425826646679	0.591662569558	-88.2719102158	-105.239250142	-206.934672152
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157	3.21055215266	-1.89945188227	2.43473176082	2.14845913777	-1.19704303601	2.23454054503	0.258191748435	0.582843923993	0.0895052399347	-0.41264216049	-0.161821264911	0.125895320728	0.0562505812685	-0.433420298835	0.0241003053309	0.306211717981	-0.0290141989613	0.104811809418	0.448641434245	0.0237876089199	0.43953357153	-140.56310245	-22.0940082734	-258.143773867
158	2.17859816606	-0.47177511535	1.77273891293	3.42871067621	-0.896898046028	1.61858212413	0.333884908698	0.111747443196	-0.43173933948	-0.33982731045	-0.622255742497	-0.525426860219	0.714948068973	0.0496739352838	-0.0864493815234	0.389254771318	0.0323276387047	0.0314919576465	0.306957864415	0.0295201938197	0.437121127018	-129.19019874	-29.8995123902	-250.863835679
159	4.45567904767	-0.243904596351	-3.12976162992	4.64789254723	1.39688887768	6.08724590609	0.0174149963386	-0.218800026625	-0.158669356589	0.333674456007	-0.224252478965	-0.307175379495	0.328335793937	0.417091164252	0.161852744151	0.213517275409	0.0187203587417	-0.0296002546654	0.387513293276	0.0702197577761	0.409979532695	-124.251735466	-22.2318918961	-247.807265355
160	3.53466941637	-1.04751675089	1.044435668	0.314288377147	-0.315452857964	0.316052216258	0.0486763256487	0.073704131792	-0.331882101895	-0.330971887141	-0.225261982651	-0.100848451645	0.411109378424	0.191883114078	0.0711651381169	0.441728006372	0.029295800214	-0.0102378826942	0.3493222234	0.0371894700323	0.420389401261	-126.861166983	-20.2657418327	-265.308703225
161	1.02787892804	0.27505584052	-0.802996386439	1.4727581316	-1.19235643915	2.45960393894	0.0766992669154	-0.292087599401	0.130804722779	0.305482046485	0.0310810029063	0.221476644066	-0.0853417662977	-0.0632505567692	-0.0851017944609	0.277283549504	-0.0793621200167	0.0831301541384	0.486968885556	0.00470427744133	0.26629588213	-138.127591263	-20.1702831239	-246.246588553
162	6.27760476834	-1.56779168578	-1.00507299385	5.84867391631	2.74419979421	6.46436670592	0.316841262119	0.345184403944	0.0526884444201	-0.334561075363	-0.264194161659	-0.290798762676	-0.201545194196	-0.263606267734	-0.143281221635	0.455354910738	-0.0109511317118	0.0587455079223	0.290349220863	0.0412357176456	0.17019635937	-129.882068221	-12.264292008	-260.38609649
163	1.20081629945	-0.350582455365	-0.142560234624	2.42114286503	-1.00386402847	0.475535119014	0.0471745245159	-0.00828552187954	0.275803630317	-0.217831932649	-0.153438706299	-0.790404432633	0.34664731333	-0.00805586185126	0.0493208935442	0.41874953476	0.165978157163	0.0359320381394	0.413002977715	0.0524974290654	0.440417875349	-84.9154575935	-45.2555275656	-272.173301303
164	1.3354332481	-0.650426683226	0.116578934865	2.641068177	-0.659960752087	1.10784249967	0.113653231994	0.08498012654	0.103933186519	-0.249986633842	-0.162488201769	-0.434253582048	0.0859647664057	-0.0917897577055	0.0217492926113	0.310996519015	0.0782968662487	0.0185358382091	0.345919941324	0.0136617305407	0.251451561812	-89.1418474916	-40.3890055418	-271.74506728
165	1.88669451935	-0.0260343107832	0.226236902729	2.38513931304	-0.11900959085	1.28638839876	0.0307640914741	-0.248362633629	0.0369755867602	0.217866890938	-0.121404186961	0.0862562129643	-0.204596672102	-0.310997839585	0.0955185183639	0.360322497297	0.0682579086511	-0.0280812899675	0.352572762179	-0.012441283175	0.259397366012	-88.6554976464	-52.6141689352	-254.89611964
166	1.52229645924	-1.09793241144	0.481233458363	3.03894392407	-0.356947215221	0.571941830609	0.0655756479487	-0.0900444159604	0.507234805153	0.00152633541913	-0.162259925513	-1.36376430964	-0.389932560806	-0.0587372049948	0.225569540712	0.402408768038	0.0106039001005	-0.0614204205193	0.320871315823	0.0823450343342	0.659033702373	-73.8752531291	-50.8771818716	-256.519925826
167	0.676934104987	0.344253449248	-0.284352085132	0.353317711957	-0.426828775999	0.500450082633	0.0601599932518	0.27038211231	0.293884701396	-0.867516851413	-0.570945000803	-1.43967579808	0.381312193515	0.604401757122	0.210435958476	0.556223307822	0.329592107646	0.568083577434	0.72314475322	0.439085908182	1.30167531244	-70.639431768	-45.8544052073	-281.299918773
168	2.73334830574	0.487510187126	-0.177700982806	3.02280466812	-1.00623792641	4.46486110261	-0.0988855606573	0.1091589772	0.406225433618	-0.171754177848	-0.376700211052	-0.0659881948382	-0.237059613219	-0.130685314534	0.37211935354	0.224947000385	0.0765887285443	-0.0179914150514	0.337381244582	-0.0153574152482	0.383827639045	-109.668662312	-16.6832554163	-269.747991511
169	5.00614715878	1.28952122102	0.0684284299664	4.55792184104	0.935362546222	2.18914699346	0.334705039023	-0.105763392392	-0.0187606616071	-0.299280503165	-0.294089858175	-0.0672358180278	-0.232891692395	-0.0660132723112	0.0349550417311	0.309324091419	0.0463896760209	0.0712165822844	0.27561499805	-0.0456476779027	0.265851565499	-102.521877736	-26.4871637522	-272.354633306
170	4.02667410826	-0.000899512689693	0.0903981101209	5.73597978963	-2.35420985468	2.08515510502	-0.00737102238543	0.139001152799	-0.286135704566	-0.112940728742	0.23815802471	0.255185257139	0.287663991655	-0.397380190851	-0.0909069961898	0.201753943409	-0.0398038975634	0.0461367853168	0.213522742544	-0.0337596034356	0.288556248117	-111.239835329	-28.7472822115	-269.33084503
171	1.88107976153	-0.544724141513	-0.688355690058	2.341503669	0.21837885885	1.80487489533	0.0836046113698	0.0265164599379	0.126952095786	-0.0601997754084	-0.21714383072	-0.0413192336119	-0.128321317711	0.0542945888516	0.0977735073333	0.320599229092	0.0382885693363	0.0197803999162	0.358355797274	-0.0086209601502	0.200385894328	-104.636875583	-21.5304461302	-269.410333751
172	0.656371886541	0.190102900598	-0.0974706774078	2.30912023624	-0.993989414864	0.42740518104	-0.00906354543803	0.109355174827	0.157990966172	-0.23979455639	-0.710636782617	-0.673072484845	-0.130415552383	0.0865705058885	0.374441339433	0.426709141285	0.256306182539	0.0331258455555	0.649590991608	0.0154279615732	0.402524776127	-142.353573728	-101.48721883	-270.007131687
173	1.65499525142	-0.681296664029	1.06969041034	2.11063618681	-0.282048592855	1.642716575	0.283610269844	0.261680250803	0.280479945269	-0.500366838599	-0.395322886712	-0.0760873529278	0.0791125072557	0.101141158976	0.0828292651543	0.346460986759	0.148587505781	0.0920625296529	0.437053716279	0.0682029402015	0.341301408696	-147.66278212	-95.5201469763	-273.022528597
174	1.20720606731	-0.725466597218	0.574456858612	2.74397131651	0.0409613138485	1.85171183698	-0.0276471858932	0.00817675201243	-0.00645878756875	0.00450435702774	-0.186408292604	0.229435017966	-0.106885920107	-0.34052804286	0.126413953695	0.277907404019	0.0349181771623	0.0158840373936	0.40618696867	-0.0489084254031	0.320376848288	-146.677589375	-109.005442538	-254.502263977
175	1.36010771403	-0.85858879952	0.458187956589	2.54722442906	-0.79654466586	0.868335231789	0.119984996369	0.0908165462051	0.411986002606	0.0870802413013	-0.398293037812	-0.594614938094	-0.041075529682	-0.171610159413	0.191076272881	0.22925220355	0.0522609988609	-0.0278710975544	0.306644657844	0.0556401455188	0.471808815615	-133.129480542	-107.906120709	-254.516786698
176	0.340186695593	-0.0169953057193	-0.578184262898	0.0544660475843	0.11153809685	1.12139882735	0.520679236131	0.400341666117	0.446063244623	-0.388057356397	-0.493988508388	-0.749323953064	0.0446394200953	0.886516543384	-0.0242849082288	0.567557328367	0.339646931939	0.317379544577	1.08955038997	0.496606298696	0.967483093084	-127.461757779	-102.12159008	-279.275160774
177	2.00714730776	0.880681697301	0.946249348947	3.89015100886	-0.583781205977	0.727621922538	0.181898502629	0.235478598817	0.448733714678	0.271141272112	-0.190947957098	0.0430347300515	0.0464200569734	-0.0370852810067	0.0749054966445	0.366503594711	0.0678561503837	0.030039922462	0.276577249049	0.0597023267626	0.426791029222	-166.948581412	-71.9856386752	-269.062701911
178	3.86439159511	-0.0881677296876	-0.243373905859	2.42515590508	0.867885748799	1.28983671242	0.27118033469	0.0916463378279	-0.0603843026853	-0.281936744088	-0.23386392855	0.254808006527	-0.145074819033	-0.133029829137	-0.0382724365342	0.342579576572	0.0428782635887	-0.0129903076976	0.282611386812	0.00569117516674	0.464335147471	-165.022924992	-81.9196284684	-272.891011519
179	1.84295610909	-0.561266809291	-0.493874080719	2.57920167271	0.575528509693	2.90469986075	0.239150997759	0.128749268384	0.158124368035	-0.0852442643217	-0.0604995119306	0.0725155308708	-0.0331169820321	0.150852059087	-0.0791309329055	0.342928674083	0.103829496685	0.00792027251691	0.39261477455	0.032832431745	0.281050517431	-160.558680982	-77.6513953629	-269.481406814

Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

ID	Refine TLS-ID	Selection details	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	chain 'A' and (resid 1 through 44 )	A	A	1 - 44	1 - 25
2	2	chain 'A' and (resid 45 through 144 )	A	A	45 - 144	26 - 105
3	3	chain 'A' and (resid 145 through 170 )	A	A	145 - 170	106 - 131
4	4	chain 'A' and (resid 171 through 187 )	A	A	171 - 187	132 - 148
5	5	chain 'A' and (resid 188 through 229 )	A	A	188 - 229	149 - 190
6	6	chain 'A' and (resid 230 through 274 )	A	A	230 - 274	191 - 235
7	7	chain 'A' and (resid 275 through 319 )	A	A	275 - 319	236 - 280
8	8	chain 'A' and (resid 320 through 349 )	A	A	320 - 349	281 - 302
9	9	chain 'A' and (resid 350 through 384 )	A	A	350 - 384	303 - 326
10	10	chain 'A' and (resid 385 through 417 )	A	A	385 - 417	327 - 352
11	11	chain 'B' and (resid 3 through 28 )	B	B	3 - 28	1 - 26
12	12	chain 'B' and (resid 29 through 60 )	B	B	29 - 60	27 - 58
13	13	chain 'B' and (resid 61 through 85 )	B	B	61 - 85	59 - 83
14	14	chain 'B' and (resid 86 through 117 )	B	B	86 - 117	84 - 115
15	15	chain 'C' and (resid 1 through 44 )	C	C	1 - 44	1 - 25
16	16	chain 'C' and (resid 45 through 144 )	C	C	45 - 144	26 - 106
17	17	chain 'C' and (resid 145 through 170 )	C	C	145 - 170	107 - 132
18	18	chain 'C' and (resid 171 through 229 )	C	C	171 - 229	133 - 191
19	19	chain 'C' and (resid 230 through 274 )	C	C	230 - 274	192 - 236
20	20	chain 'C' and (resid 275 through 319 )	C	C	275 - 319	237 - 281
21	21	chain 'C' and (resid 320 through 342 )	C	C	320 - 342	282 - 297
22	22	chain 'C' and (resid 343 through 384 )	C	C	343 - 384	298 - 333
23	23	chain 'C' and (resid 385 through 417 )	C	C	385 - 417	334 - 361
24	24	chain 'D' and (resid 3 through 28 )	D	D	3 - 28	1 - 26
25	25	chain 'D' and (resid 29 through 60 )	D	D	29 - 60	27 - 58
26	26	chain 'D' and (resid 61 through 77 )	D	D	61 - 77	59 - 75
27	27	chain 'D' and (resid 78 through 117 )	D	D	78 - 117	76 - 115
28	28	chain 'E' and (resid 1 through 44 )	E	E	1 - 44	1 - 25
29	29	chain 'E' and (resid 45 through 144 )	E	E	45 - 144	26 - 106
30	30	chain 'E' and (resid 145 through 319 )	E	E	145 - 319	107 - 281
31	31	chain 'E' and (resid 320 through 417 )	E	E	320 - 417	282 - 368
32	32	chain 'F' and (resid 3 through 18 )	F	F	3 - 18	1 - 16
33	33	chain 'F' and (resid 19 through 28 )	F	F	19 - 28	17 - 26
34	34	chain 'F' and (resid 29 through 60 )	F	F	29 - 60	27 - 58
35	35	chain 'F' and (resid 61 through 77 )	F	F	61 - 77	59 - 75
36	36	chain 'F' and (resid 78 through 85 )	F	F	78 - 85	76 - 83
37	37	chain 'F' and (resid 86 through 110 )	F	F	86 - 110	84 - 108
38	38	chain 'F' and (resid 111 through 117 )	F	F	111 - 117	109 - 115
39	39	chain 'G' and (resid 1 through 64 )	G	G	1 - 64	1 - 45
40	40	chain 'G' and (resid 65 through 144 )	G	G	65 - 144	46 - 106
41	41	chain 'G' and (resid 145 through 274 )	G	G	145 - 274	107 - 236
42	42	chain 'G' and (resid 275 through 319 )	G	G	275 - 319	237 - 281
43	43	chain 'G' and (resid 320 through 384 )	G	G	320 - 384	282 - 339
44	44	chain 'G' and (resid 385 through 417 )	G	G	385 - 417	340 - 366
45	45	chain 'H' and (resid 3 through 28 )	H	H	3 - 28	1 - 26
46	46	chain 'H' and (resid 29 through 60 )	H	H	29 - 60	27 - 58
47	47	chain 'H' and (resid 61 through 77 )	H	H	61 - 77	59 - 75
48	48	chain 'H' and (resid 78 through 117 )	H	H	78 - 117	76 - 115
49	49	chain 'I' and (resid 1 through 44 )	I	I	1 - 44	1 - 25
50	50	chain 'I' and (resid 45 through 157 )	I	I	45 - 157	26 - 117
51	51	chain 'I' and (resid 158 through 229 )	I	I	158 - 229	118 - 189
52	52	chain 'I' and (resid 230 through 349 )	I	I	230 - 349	190 - 301
53	53	chain 'I' and (resid 350 through 417 )	I	I	350 - 417	302 - 358
54	54	chain 'J' and (resid 3 through 18 )	J	J	3 - 18	1 - 16
55	55	chain 'J' and (resid 19 through 28 )	J	J	19 - 28	17 - 26
56	56	chain 'J' and (resid 29 through 60 )	J	J	29 - 60	27 - 58
57	57	chain 'J' and (resid 61 through 77 )	J	J	61 - 77	59 - 75
58	58	chain 'J' and (resid 78 through 85 )	J	J	78 - 85	76 - 83
59	59	chain 'J' and (resid 86 through 110 )	J	J	86 - 110	84 - 108
60	60	chain 'J' and (resid 111 through 117 )	J	J	111 - 117	109 - 115
61	61	chain 'K' and (resid 1 through 144 )	K	K	1 - 144	1 - 105
62	62	chain 'K' and (resid 145 through 341 )	K	K	145 - 341	106 - 300
63	63	chain 'K' and (resid 342 through 417 )	K	K	342 - 417	301 - 376
64	64	chain 'L' and (resid 3 through 28 )	L	L	3 - 28	1 - 26
65	65	chain 'L' and (resid 29 through 60 )	L	L	29 - 60	27 - 58
66	66	chain 'L' and (resid 61 through 77 )	L	L	61 - 77	59 - 75
67	67	chain 'L' and (resid 78 through 117 )	L	L	78 - 117	76 - 115
68	68	chain 'M' and (resid 1 through 44 )	M	M	1 - 44	1 - 25
69	69	chain 'M' and (resid 45 through 144 )	M	M	45 - 144	26 - 105
70	70	chain 'M' and (resid 145 through 170 )	M	M	145 - 170	106 - 131
71	71	chain 'M' and (resid 171 through 229 )	M	M	171 - 229	132 - 190
72	72	chain 'M' and (resid 230 through 274 )	M	M	230 - 274	191 - 235
73	73	chain 'M' and (resid 275 through 319 )	M	M	275 - 319	236 - 280
74	74	chain 'M' and (resid 320 through 355 )	M	M	320 - 355	281 - 314
75	75	chain 'M' and (resid 356 through 384 )	M	M	356 - 384	315 - 339
76	76	chain 'M' and (resid 385 through 417 )	M	M	385 - 417	340 - 372
77	77	chain 'N' and (resid 3 through 28 )	N	N	3 - 28	1 - 26
78	78	chain 'N' and (resid 29 through 77 )	N	N	29 - 77	27 - 75
79	79	chain 'N' and (resid 78 through 110 )	N	N	78 - 110	76 - 108
80	80	chain 'N' and (resid 111 through 117 )	N	N	111 - 117	109 - 115
81	81	chain 'O' and (resid 1 through 44 )	O	O	1 - 44	1 - 25
82	82	chain 'O' and (resid 45 through 144 )	O	O	45 - 144	26 - 105
83	83	chain 'O' and (resid 145 through 229 )	O	O	145 - 229	106 - 190
84	84	chain 'O' and (resid 230 through 349 )	O	O	230 - 349	191 - 303
85	85	chain 'O' and (resid 350 through 417 )	O	O	350 - 417	304 - 361
86	86	chain 'P' and (resid 3 through 28 )	P	P	3 - 28	1 - 26
87	87	chain 'P' and (resid 29 through 67 )	P	P	29 - 67	27 - 65
88	88	chain 'P' and (resid 68 through 85 )	P	P	68 - 85	66 - 83
89	89	chain 'P' and (resid 86 through 110 )	P	P	86 - 110	84 - 108
90	90	chain 'P' and (resid 111 through 117 )	P	P	111 - 117	109 - 115
91	91	chain 'Q' and (resid 1 through 44 )	Q	Q	1 - 44	1 - 25
92	92	chain 'Q' and (resid 45 through 157 )	Q	Q	45 - 157	26 - 117
93	93	chain 'Q' and (resid 158 through 229 )	Q	Q	158 - 229	118 - 189
94	94	chain 'Q' and (resid 230 through 274 )	Q	Q	230 - 274	190 - 234
95	95	chain 'Q' and (resid 275 through 319 )	Q	Q	275 - 319	235 - 279
96	96	chain 'Q' and (resid 320 through 417 )	Q	Q	320 - 417	280 - 375
97	97	chain 'R' and (resid 3 through 9 )	R	R	3 - 9	1 - 7
98	98	chain 'R' and (resid 10 through 18 )	R	R	10 - 18	8 - 16
99	99	chain 'R' and (resid 19 through 42 )	R	R	19 - 42	17 - 40
100	100	chain 'R' and (resid 43 through 67 )	R	R	43 - 67	41 - 65
101	101	chain 'R' and (resid 68 through 85 )	R	R	68 - 85	66 - 83
102	102	chain 'R' and (resid 86 through 117 )	R	R	86 - 117	84 - 115
103	103	chain 'S' and (resid 1 through 44 )	S	S	1 - 44	1 - 25
104	104	chain 'S' and (resid 45 through 101 )	S	S	45 - 101	26 - 82
105	105	chain 'S' and (resid 102 through 229 )	S	S	102 - 229	83 - 189
106	106	chain 'S' and (resid 230 through 274 )	S	S	230 - 274	190 - 234
107	107	chain 'S' and (resid 275 through 319 )	S	S	275 - 319	235 - 279
108	108	chain 'S' and (resid 320 through 415 )	S	S	320 - 415	280 - 345
109	109	chain 'T' and (resid 3 through 18 )	T	T	3 - 18	1 - 16
110	110	chain 'T' and (resid 19 through 28 )	T	T	19 - 28	17 - 26
111	111	chain 'T' and (resid 29 through 50 )	T	T	29 - 50	27 - 48
112	112	chain 'T' and (resid 51 through 60 )	T	T	51 - 60	49 - 58
113	113	chain 'T' and (resid 61 through 85 )	T	T	61 - 85	59 - 83
114	114	chain 'T' and (resid 86 through 110 )	T	T	86 - 110	84 - 108
115	115	chain 'T' and (resid 111 through 117 )	T	T	111 - 117	109 - 115
116	116	chain 'U' and (resid 1 through 44 )	U	U	1 - 44	1 - 25
117	117	chain 'U' and (resid 45 through 144 )	U	U	45 - 144	26 - 105
118	118	chain 'U' and (resid 145 through 247 )	U	U	145 - 247	106 - 208
119	119	chain 'U' and (resid 248 through 274 )	U	U	248 - 274	209 - 235
120	120	chain 'U' and (resid 275 through 319 )	U	U	275 - 319	236 - 280
121	121	chain 'U' and (resid 320 through 384 )	U	U	320 - 384	281 - 324
122	122	chain 'U' and (resid 385 through 417 )	U	U	385 - 417	325 - 348
123	123	chain 'V' and (resid 3 through 18 )	V	V	3 - 18	1 - 16
124	124	chain 'V' and (resid 19 through 42 )	V	V	19 - 42	17 - 40
125	125	chain 'V' and (resid 43 through 60 )	V	V	43 - 60	41 - 58
126	126	chain 'V' and (resid 61 through 77 )	V	V	61 - 77	59 - 75
127	127	chain 'V' and (resid 78 through 91 )	V	V	78 - 91	76 - 89
128	128	chain 'V' and (resid 92 through 117 )	V	V	92 - 117	90 - 115
129	129	chain 'W' and (resid 1 through 94 )	W	W	1 - 94	1 - 75
130	130	chain 'W' and (resid 95 through 144 )	W	W	95 - 144	76 - 105
131	131	chain 'W' and (resid 145 through 229 )	W	W	145 - 229	106 - 190
132	132	chain 'W' and (resid 230 through 349 )	W	W	230 - 349	191 - 301
133	133	chain 'W' and (resid 350 through 417 )	W	W	350 - 417	302 - 350
134	134	chain 'X' and (resid 3 through 28 )	X	X	3 - 28	1 - 26
135	135	chain 'X' and (resid 29 through 60 )	X	X	29 - 60	27 - 58
136	136	chain 'X' and (resid 61 through 85 )	X	X	61 - 85	59 - 83
137	137	chain 'X' and (resid 86 through 117 )	X	X	86 - 117	84 - 115
138	138	chain 'Y' and (resid 1 through 44 )	Y	Y	1 - 44	1 - 25
139	139	chain 'Y' and (resid 45 through 144 )	Y	Y	45 - 144	26 - 105
140	140	chain 'Y' and (resid 145 through 274 )	Y	Y	145 - 274	106 - 235
141	141	chain 'Y' and (resid 275 through 319 )	Y	Y	275 - 319	236 - 280
142	142	chain 'Y' and (resid 320 through 395 )	Y	Y	320 - 395	281 - 354
143	143	chain 'Y' and (resid 396 through 417 )	Y	Y	396 - 417	355 - 376
144	144	chain 'Z' and (resid 3 through 18 )	Z	Z	3 - 18	1 - 16
145	145	chain 'Z' and (resid 19 through 42 )	Z	Z	19 - 42	17 - 40
146	146	chain 'Z' and (resid 43 through 117 )	Z	Z	43 - 117	41 - 115
147	147	chain 'a' and (resid 1 through 44 )	a	AA	1 - 44	1 - 25
148	148	chain 'a' and (resid 45 through 229 )	a	AA	45 - 229	26 - 190
149	149	chain 'a' and (resid 230 through 274 )	a	AA	230 - 274	191 - 235
150	150	chain 'a' and (resid 275 through 319 )	a	AA	275 - 319	236 - 280
151	151	chain 'a' and (resid 320 through 334 )	a	AA	320 - 334	281 - 295
152	152	chain 'a' and (resid 339 through 417 )	a	AA	339 - 417	296 - 357
153	153	chain 'b' and (resid 3 through 9 )	b	AB	3 - 9	1 - 7
154	154	chain 'b' and (resid 10 through 18 )	b	AB	10 - 18	8 - 16
155	155	chain 'b' and (resid 19 through 28 )	b	AB	19 - 28	17 - 26
156	156	chain 'b' and (resid 29 through 42 )	b	AB	29 - 42	27 - 40
157	157	chain 'b' and (resid 43 through 50 )	b	AB	43 - 50	41 - 48
158	158	chain 'b' and (resid 51 through 67 )	b	AB	51 - 67	49 - 65
159	159	chain 'b' and (resid 68 through 85 )	b	AB	68 - 85	66 - 83
160	160	chain 'b' and (resid 86 through 91 )	b	AB	86 - 91	84 - 89
161	161	chain 'b' and (resid 92 through 110 )	b	AB	92 - 110	90 - 108
162	162	chain 'b' and (resid 111 through 117 )	b	AB	111 - 117	109 - 115
163	163	chain 'c' and (resid 1 through 44 )	c	IB	1 - 44	1 - 25
164	164	chain 'c' and (resid 45 through 157 )	c	IB	45 - 157	26 - 117
165	165	chain 'c' and (resid 158 through 229 )	c	IB	158 - 229	118 - 189
166	166	chain 'c' and (resid 230 through 349 )	c	IB	230 - 349	190 - 302
167	167	chain 'c' and (resid 350 through 417 )	c	IB	350 - 417	303 - 352
168	168	chain 'd' and (resid 3 through 28 )	d	JB	3 - 28	1 - 26
169	169	chain 'd' and (resid 29 through 60 )	d	JB	29 - 60	27 - 58
170	170	chain 'd' and (resid 61 through 77 )	d	JB	61 - 77	59 - 75
171	171	chain 'd' and (resid 78 through 117 )	d	JB	78 - 117	76 - 115
172	172	chain 'e' and (resid 1 through 44 )	e	KB	1 - 44	1 - 25
173	173	chain 'e' and (resid 45 through 144 )	e	KB	45 - 144	26 - 105
174	174	chain 'e' and (resid 145 through 229 )	e	KB	145 - 229	106 - 190
175	175	chain 'e' and (resid 230 through 349 )	e	KB	230 - 349	191 - 301
176	176	chain 'e' and (resid 350 through 417 )	e	KB	350 - 417	302 - 354
177	177	chain 'f' and (resid 3 through 18 )	f	LB	3 - 18	1 - 16
178	178	chain 'f' and (resid 19 through 85 )	f	LB	19 - 85	17 - 83
179	179	chain 'f' and (resid 86 through 117 )	f	LB	86 - 117	84 - 115