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Open data
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Basic information
Entry | Database: PDB / ID: 7rfz | ||||||
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Title | Importin alpha 2 in complex with MERS ORF4B NLS peptide | ||||||
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![]() | TRANSPORT PROTEIN/Viral Protein / imortin / ![]() ![]() ![]() | ||||||
Function / homology | ![]() Sensing of DNA Double Strand Breaks / entry of viral genome into host nucleus through nuclear pore complex via importin / positive regulation of viral life cycle / NLS-dependent protein nuclear import complex / postsynapse to nucleus signaling pathway / nuclear import signal receptor activity / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Munasinghe, T.S. / Tsimbalyuk, S. / Roby, J.A. / Aragao, D. / Forwood, J.K. | ||||||
Funding support | ![]()
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![]() | ![]() Title: MERS-CoV ORF4b employs an unusual binding mechanism to target IMP alpha and block innate immunity. Authors: Munasinghe, T.S. / Edwards, M.R. / Tsimbalyuk, S. / Vogel, O.A. / Smith, K.M. / Stewart, M. / Foster, J.K. / Bosence, L.A. / Aragao, D. / Roby, J.A. / Basler, C.F. / Forwood, J.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 219.2 KB | Display | ![]() |
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PDB format | ![]() | 140.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7rfxC ![]() 7rfyC ![]() 7rg0C ![]() 7rg2C ![]() 7rg3C ![]() 7rg4C ![]() 7rg5C ![]() 7rg6C ![]() 6bvtS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 55268.473 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 2711.312 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Production host: ![]() ![]() ![]() |
#3: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.03 Å3/Da / Density % sol: 59.44 % |
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Crystal grow![]() | Temperature: 296 K / Method: vapor diffusion, hanging drop / Details: 0.65 M Sodium Citrate, 0.1 M HEPES, pH 7.0 |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Dec 6, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.95→29.84 Å / Num. obs: 52136 / % possible obs: 100 % / Redundancy: 13.1 % / Biso Wilson estimate: 34.65 Å2 / Rmerge(I) obs: 0.1 / Net I/σ(I): 14.4 |
Reflection shell | Resolution: 1.95→2 Å / Rmerge(I) obs: 1.55 / Mean I/σ(I) obs: 2 / Num. unique obs: 3622 / Rsym value: 0.64 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 6BVT Resolution: 1.95→29.51 Å / SU ML: 0.2269 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 22.1226 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 49.87 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.95→29.51 Å
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Refine LS restraints |
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LS refinement shell |
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