pectinesterase / pectinesterase activity / : / cell wall modification / pectin catabolic process / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein-RNA complex assembly / MAP kinase activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation regulator activity ...pectinesterase / pectinesterase activity / : / cell wall modification / pectin catabolic process / endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / protein-RNA complex assembly / MAP kinase activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation regulator activity / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / maturation of SSU-rRNA / small-subunit processome / modification-dependent protein catabolic process / rRNA processing / protein tag activity / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit rRNA binding / リボソーム生合成 / ribosome binding / 5S rRNA binding / large ribosomal subunit rRNA binding / small ribosomal subunit / cytosolic small ribosomal subunit / cytosolic large ribosomal subunit / cytoplasmic translation / negative regulation of translation / rRNA binding / リボソーム / protein ubiquitination / intracellular signal transduction / structural constituent of ribosome / ribonucleoprotein complex / 翻訳 (生物学) / mRNA binding / ubiquitin protein ligase binding / 核小体 / RNA binding / zinc ion binding / ATP binding / 生体膜 / metal ion binding / 細胞核 / 細胞質基質 / 細胞質 類似検索 - 分子機能
SPARK domain / スパーク / Pectinesterase, Asp active site / Pectinesterase signature 2. / Pectinesterase, catalytic / Pectinesterase / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Pectin lyase fold / Ribosomal protein L23 ...SPARK domain / スパーク / Pectinesterase, Asp active site / Pectinesterase signature 2. / Pectinesterase, catalytic / Pectinesterase / Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Pectin lyase fold / Ribosomal protein L23 / Ribosomal protein L28e / Ribosomal protein L41 / Ribosomal protein L41 / : / Pectin lyase fold/virulence factor / metallochaperone-like domain / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / TRASH domain / Ribosomal protein S21e, conserved site / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S19e, conserved site / Ribosomal protein S26e / Ribosomal protein L29e / Ribosomal protein S26e signature. / Ribosomal protein S2, eukaryotic / Ribosomal L29e protein family / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S21e signature. / Ribosomal protein S2, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S8e, conserved site / Ribosomal protein L24e, conserved site / Ribosomal protein S4e, N-terminal, conserved site / 40S ribosomal protein S4, C-terminal domain / Ribosomal protein S19e signature. / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S19e / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal protein L23/L25, N-terminal / Ribosomal L22e protein family / 40S ribosomal protein S4 C-terminus / Ribosomal protein S4e, N-terminal / Ribosomal protein L34e, conserved site / Ribosomal protein S23, eukaryotic/archaeal / 40S Ribosomal protein S10 / Ribosomal protein L23, N-terminal domain / Ribosomal protein S10, eukaryotic/archaeal / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein S8e / Mitogen-activated protein (MAP) kinase, conserved site / MAP kinase signature. / Ribosomal protein S4, KOW domain / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal Protein L6, KOW domain / Ribosomal protein S25 / S25 ribosomal protein / RS4NT (NUC023) domain / : / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein S17e, conserved site / Ribosomal protein S17e signature. / Ribosomal protein L14e domain / 60S ribosomal protein L6E / Ribosomal protein S30 / Ribosomal protein S30 / Ribosomal protein L34Ae / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L44e signature. / Ribosomal family S4e / Ribosomal protein S7e signature. / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L32e, conserved site / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein L10e / Ribosomal protein L19, eukaryotic / Ribosomal protein L7A/L8 / Ribosomal protein L34e / 60S ribosomal protein L4, C-terminal domain / Ribosomal protein L7, eukaryotic / Ribosomal protein L6e 類似検索 - ドメイン・相同性
グルコース / : / プトレシン / スペルミジン / スペルミン / : / : / : / リボ核酸 / RNA (> 10) ...グルコース / : / プトレシン / スペルミジン / スペルミン / : / : / : / リボ核酸 / RNA (> 10) / RNA (> 100) / RNA (> 1000) / 40S ribosomal protein S30 / 40S ribosomal protein S15a-1 / 40S ribosomal protein S27 / Small ribosomal subunit protein uS10 domain-containing protein / 60S ribosomal protein L32 / 40S ribosomal protein S25 / 40S ribosomal protein S15 / KOW domain-containing protein / 40S ribosomal protein S18 / Uncharacterized protein / Uncharacterized protein / 60S ribosomal protein L35a / 40S ribosomal protein S19 / Ribosomal protein L37 / Ribosomal eL28/Mak16 domain-containing protein / S5 DRBM domain-containing protein / 40S ribosomal protein S21 / 40S ribosomal protein S16 / 60S ribosomal protein L34 / 60S ribosomal protein L29 / Large ribosomal subunit protein uL6 N-terminal domain-containing protein / Ribosomal protein L19 / 40S ribosomal protein S4 / 40S ribosomal protein S26 / 60S ribosomal protein L36 / Ribosomal protein eL8/eL30/eS12/Gadd45 domain-containing protein / Protein kinase domain-containing protein / Ribosomal_L18e/L15P domain-containing protein / 60S ribosomal protein L27 / 40S ribosomal protein S7 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein uL18 C-terminal eukaryotes domain-containing protein / KH type-2 domain-containing protein / Pectinesterase / 40S ribosomal protein S8 / Ribosomal protein L15 / 40S ribosomal protein S6 / Large ribosomal subunit protein uL4 C-terminal domain-containing protein / 40S ribosomal protein S24 / Large ribosomal subunit protein uL15/eL18 domain-containing protein / 60S ribosomal protein L22 / Small ribosomal subunit protein eS1 / 60S ribosomal protein L18a / 60S ribosomal protein L21 / TRASH domain-containing protein / Large ribosomal subunit protein uL23 N-terminal domain-containing protein / Uncharacterized protein / Small ribosomal subunit protein uS7 domain-containing protein / Large ribosomal subunit protein eL14 domain-containing protein / 60S ribosomal protein L39 / 60S ribosomal protein L13 / Large ribosomal subunit protein uL6 alpha-beta domain-containing protein / 60S ribosomal protein L31 / Plectin/eS10 N-terminal domain-containing protein / Uncharacterized protein / Ubiquitin-like domain-containing protein / 40S ribosomal protein S28 / 60S ribosomal protein L41 / Small ribosomal subunit protein uS17 N-terminal domain-containing protein / 60S ribosomal protein L11 / 60S ribosomal protein L37a / 60S ribosomal protein L23 / Small ribosomal subunit protein uS15 N-terminal domain-containing protein / 30S ribosomal protein S12, chloroplastic / Thaliana 60S ribosomal protein L7 / Large ribosomal subunit protein uL2 / Large ribosomal subunit protein eL38 / Small ribosomal subunit protein eS17 / Ribosomal protein S14 / 60S ribosomal protein L10 / Similar to 60S ribosomal protein L35 / Ribosomal protein L3 類似検索 - 構成要素
ジャーナル: Plant Commun / 年: 2022 タイトル: Cryo-EM structure and rRNA modification sites of a plant ribosome. 著者: Patrick Cottilli / Yuzuru Itoh / Yuko Nobe / Anton S Petrov / Purificación Lisón / Masato Taoka / Alexey Amunts / 要旨: Protein synthesis in crop plants contributes to the balance of food and fuel on our planet, which influences human metabolic activity and lifespan. Protein synthesis can be regulated with respect to ...Protein synthesis in crop plants contributes to the balance of food and fuel on our planet, which influences human metabolic activity and lifespan. Protein synthesis can be regulated with respect to changing environmental cues via the deposition of chemical modifications into rRNA. Here, we present the structure of a plant ribosome from tomato and a quantitative mass spectrometry analysis of its rRNAs. The study reveals fine features of the ribosomal proteins and 71 plant-specific rRNA modifications, and it re-annotates 30 rRNA residues in the available sequence. At the protein level, isoAsp is found in position 137 of uS11, and a zinc finger previously believed to be universal is missing from eL34, suggesting a lower effect of zinc deficiency on protein synthesis in plants. At the rRNA level, the plant ribosome differs markedly from its human counterpart with respect to the spatial distribution of modifications. Thus, it represents an additional layer of gene expression regulation, highlighting the molecular signature of a plant ribosome. The results provide a reference model of a plant ribosome for structural studies and an accurate marker for molecular ecology.
D: 60S ribosomal protein L8 F: Ribos_L4_asso_C domain-containing protein E: Ribosomal protein L3 G: Ribosomal_L18_c domain-containing protein H: Ribosomal_L6e_N domain-containing protein I: Thaliana 60S ribosomal protein L7 J: Ribosomal_L7Ae domain-containing protein K: 60S ribosomal protein uL6 L: 60S ribosomal protein L10 M: 60S ribosomal protein uL5 N: 60S ribosomal protein L13 O: Ribosomal_L14e domain-containing protein P: Ribosomal protein L15 Q: Pectinesterase R: 50S ribosomal protein L22, chloroplastic S: Ribosomal_L18e/L15P domain-containing protein T: Ribosomal protein L19 U: 60S ribosomal protein L18a V: 60S ribosomal protein eL21 W: 60S ribosomal protein eL22 X: 60S ribosomal protein uL14 Y: TRASH domain-containing protein Z: Ribosomal_L23eN domain-containing protein a: KOW domain-containing protein b: 60S ribosomal protein L27 c: Ribosomal_L18e/L15P domain-containing protein d: 60S ribosomal protein L29 e: 60S ribosomal protein eL30 f: 60S ribosomal protein eL31 g: 60S ribosomal protein eL32 h: 60S ribosomal protein eL33 i: 60S ribosomal protein eL34 j: Similar to 60S ribosomal protein L35 k: 60S ribosomal protein L36 l: Ribosomal protein L37 m: 60S ribosomal protein L38 n: 60S ribosomal protein eL39 o: Ubiquitin p: 60S ribosomal protein eL42 q: 60S ribosomal protein eL43 r: Ribosomal_L28e domain-containing protein s: tRNA 2: 25S rRNA 5: 5S rRNA 8: 5.8S rRNA S2: 18S NA: KH type-2 domain-containing protein OA: Ribosomal_S7 domain-containing protein PA: S10_plectin domain-containing protein QA: 40S ribosomal protein uS19 RA: 40S ribosomal protein uS9 TA: 40S ribosomal protein uS13 UA: 40S ribosomal protein eS19 VA: Ribosomal_S10 domain-containing protein WA: 40S ribosomal protein eS28 XA: 40S ribosomal protein uS14 YA: Mitogen-activated protein kinase ZA: 40S ribosomal protein S25 aA: tRNA_1 bA: mRNA t: 60S ribosomal protein L41 u: 40S ribosomal protein SA v: 40S ribosomal protein S3a w: 40S ribosomal protein S4 x: 40S ribosomal protein S7 y: 40S ribosomal protein S8 z: Ribosomal_S17_N domain-containing protein AA: 40S ribosomal protein S17 BA: 40S ribosomal protein S21 CA: 40S body ribosomal protein uS12 DA: 40S ribosomal protein S26 EA: S5 DRBM domain-containing protein FA: 40S ribosomal protein S6 GA: 40S body ribosomal protein uS4 HA: 30S ribosomal protein S15, chloroplastic IA: Ribosomal protein S14 KA: 40S ribosomal protein S24 LA: 40S ribosomal protein S27 MA: 40S ribosomal protein S30 JA: 40S ribosomal protein S15a-1 ヘテロ分子