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- PDB-7pw2: Crystal structure of the Abl SH3 domain V73E-A74S-S75R-G76T-D77E ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7pw2 | ||||||
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Title | Crystal structure of the Abl SH3 domain V73E-A74S-S75R-G76T-D77E mutant | ||||||
![]() | Non-specific protein-tyrosine kinase![]() | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
Model details | Chimera construction Abl-c-Src SH3 domain | ||||||
![]() | Camara-Artigas, A. / Salinas Garcia, M.C. | ||||||
Funding support | ![]()
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![]() | ![]() Title: The effect of the hinge loops composition in the domain swapping of the SH3 domain Authors: Camara-Artigas, A. / Salinas Garcia, M.C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 45.4 KB | Display | ![]() |
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PDB format | ![]() | 30.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7pvqC ![]() 7pvrC ![]() 7pvsC ![]() 7pvvC ![]() 7pvwC ![]() 7pvyC ![]() 7pvzC ![]() 7pw0C ![]() 3eg3S S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 6721.397 Da / Num. of mol.: 1 / Mutation: V73E, A74S, S75R, G76T, D77E Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: UniProt: A9UF07, ![]() |
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#2: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.05 Å3/Da / Density % sol: 40.02 % / Mosaicity: 0.09 ° |
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 7 / Details: 2.0 M ammonium sulfate, 0.1M HEPES |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 2, 2017 | ||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength![]() | ||||||||||||||||||||||||||||||
Reflection | Resolution: 1.1→17.44 Å / Num. obs: 21193 / % possible obs: 95.3 % / Redundancy: 2 % / Biso Wilson estimate: 17.78 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.027 / Rpim(I) all: 0.022 / Rrim(I) all: 0.035 / Net I/σ(I): 9.9 / Num. measured all: 43012 | ||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Phasing
Phasing![]() | Method: ![]() |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 3EG3 Resolution: 1.1→17.44 Å / SU ML: 0.12 / Cross valid method: THROUGHOUT / σ(F): 0.32 / Phase error: 34.68 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 64.6 Å2 / Biso mean: 27.5203 Å2 / Biso min: 14.31 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.1→17.44 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 13
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