[English] 日本語
![](img/lk-miru.gif)
- PDB-7pvv: Crystal structure of the Abl SH3 domain G92N-Y93N-N94T-H95E mutant -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 7pvv | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of the Abl SH3 domain G92N-Y93N-N94T-H95E mutant | ||||||
![]() | Bcr-abl1 e6a2 chimeric protein | ||||||
![]() | ![]() ![]() ![]() | ||||||
Function / homology | ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
Model details | Chimera construction Abl-c-Src SH3 domain | ||||||
![]() | Camara-Artigas, A. / Salinas Garcia, M.C. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: The effect of the hinge loops composition in the domain swapping of the SH3 domain Authors: Camara-Artigas, A. / Salinas Garcia, M.C. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 48 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 33.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 7pvqC ![]() 7pvrC ![]() 7pvsC ![]() 7pvwC ![]() 7pvyC ![]() 7pvzC ![]() 7pw0C ![]() 7pw2C ![]() 3eg3S S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Components on special symmetry positions |
|
-
Components
#1: Protein | Mass: 6476.116 Da / Num. of mol.: 1 / Mutation: G92N, Y93N, N94T, H95E Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
---|---|
#2: Chemical | ChemComp-PEG / ![]() |
#3: Chemical | ChemComp-PGE / ![]() |
#4: Water | ChemComp-HOH / ![]() |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.89 Å3/Da / Density % sol: 57.45 % / Mosaicity: 0.07 ° |
---|---|
Crystal grow![]() | Temperature: 283 K / Method: vapor diffusion, sitting drop / pH: 5.5 Details: 1.6 M ammonium sulfate, 5% PEG200, 10% Glicerol, 40 mM LiCl, 0.1M Sodium Acetate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Sep 11, 2019 | ||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength![]() | ||||||||||||||||||||||||||||||
Reflection | Resolution: 1.82→19.9 Å / Num. obs: 7019 / % possible obs: 99.8 % / Redundancy: 4.8 % / Biso Wilson estimate: 28.15 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.038 / Rpim(I) all: 0.019 / Rrim(I) all: 0.042 / Net I/σ(I): 14.8 / Num. measured all: 33491 | ||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
|
-Phasing
Phasing![]() | Method: ![]() |
---|
-
Processing
Software |
| ||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure![]() ![]() Starting model: 3EG3 Resolution: 1.82→19.9 Å / SU ML: 0.2 / Cross valid method: THROUGHOUT / σ(F): 0.51 / Phase error: 32.98 / Stereochemistry target values: ML
| ||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 98.44 Å2 / Biso mean: 39.7719 Å2 / Biso min: 20.03 Å2 | ||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.82→19.9 Å
| ||||||||||||||||||||||||||||||
LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / % reflection obs: 99 %
| ||||||||||||||||||||||||||||||
Refinement TLS group |
|