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- PDB-7o0q: Crystal structure of the human METTL3-METTL14 complex bound to Co... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7o0q | ||||||
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Title | Crystal structure of the human METTL3-METTL14 complex bound to Compound 12 (ADO_AD_066) | ||||||
![]() | (N6-adenosine-methyltransferase ...) x 2 | ||||||
![]() | ![]() ![]() ![]() | ||||||
Function / homology | ![]() negative regulation of hematopoietic progenitor cell differentiation / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Bedi, R.K. / Dolbois, A. / Caflisch, A. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of the human METTL3-METTL14 complex bound to Compound 12 (ADO_AD_066) Authors: Bedi, R.K. / Dolbois, A. / Caflisch, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 126.5 KB | Display | ![]() |
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PDB format | ![]() | 76.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7acdS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-N6-adenosine-methyltransferase ... , 2 types, 2 molecules AB
#1: Protein | Mass: 28144.080 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#2: Protein | Mass: 33621.246 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
-Non-polymers , 4 types, 54 molecules ![](data/chem/img/UY2.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-UY2 / |
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#4: Chemical | ChemComp-MG / |
#5: Chemical | ChemComp-ACT / ![]() |
#6: Water | ChemComp-HOH / ![]() |
-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.17 Å3/Da / Density % sol: 43.37 % |
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Crystal grow![]() | Temperature: 295 K / Method: vapor diffusion, hanging drop / Details: 20% PEG 3350, 400mM Mg acetate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M-F / Detector: PIXEL / Date: Oct 18, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.49→45.143 Å / Num. obs: 19754 / % possible obs: 99.8 % / Redundancy: 9.56 % / Biso Wilson estimate: 60.71 Å2 / CC1/2: 0.998 / Net I/σ(I): 12.89 |
Reflection shell | Resolution: 2.49→2.64 Å / Num. unique obs: 3099 / CC1/2: 0.66 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 7ACD Resolution: 2.49→44.7 Å / SU ML: 0.3852 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 29.778 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 64.32 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.49→44.7 Å
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Refine LS restraints |
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LS refinement shell |
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