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Yorodumi- PDB-7nwn: A carbohydrate binding module family 9 (CBM9) from Caldicellulsir... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7nwn | |||||||||||||||
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Title | A carbohydrate binding module family 9 (CBM9) from Caldicellulsiruptor kristjanssonii | |||||||||||||||
Components | Beta-xylanaseXylanase | |||||||||||||||
Keywords | SUGAR BINDING PROTEIN / CARBOHYDRATE BINDING MODULE | |||||||||||||||
Function / homology | Function and homology information endo-1,4-beta-xylanase activity / endo-1,4-beta-xylanase / polysaccharide catabolic process / carbohydrate binding Similarity search - Function | |||||||||||||||
Biological species | Caldicellulosiruptor kristjanssonii | |||||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.97 Å | |||||||||||||||
Authors | Krska, D. / Mazurkewich, S. / Navarro Poulsen, J. / Larsbrink, J. / Lo Leggio, L. | |||||||||||||||
Funding support | Sweden, Denmark, 4items
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Citation | Journal: Biochemistry / Year: 2021 Title: Structural and Functional Analysis of a Multimodular Hyperthermostable Xylanase-Glucuronoyl Esterase from Caldicellulosiruptor kristjansonii . Authors: Krska, D. / Mazurkewich, S. / Brown, H.A. / Theibich, Y. / Poulsen, J.N. / Morris, A.L. / Koropatkin, N.M. / Lo Leggio, L. / Larsbrink, J. | |||||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7nwn.cif.gz | 64.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7nwn.ent.gz | Display | PDB format | |
PDBx/mmJSON format | 7nwn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/nw/7nwn ftp://data.pdbj.org/pub/pdb/validation_reports/nw/7nwn | HTTPS FTP |
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-Related structure data
Related structure data | 7nn3C 7nwoC 7nwpC 7nwqC 1i8uS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
-Protein , 1 types, 1 molecules AAA
#1: Protein | Mass: 24645.232 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Caldicellulosiruptor kristjanssonii (strain ATCC 700853 / DSM 12137 / I77R1B) (bacteria) Strain: ATCC 700853 / DSM 12137 / I77R1B / Gene: Calkr_2245 / Production host: Escherichia coli BL21 (bacteria) / References: UniProt: E4S6E9, endo-1,4-beta-xylanase |
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-Non-polymers , 5 types, 159 molecules
#2: Chemical | ChemComp-PEG / #3: Chemical | ChemComp-EDO / #4: Chemical | #5: Chemical | ChemComp-P6G / | #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.35 Å3/Da / Density % sol: 71.74 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.1 M phosphate-citrate and 40% polyethylene glycol 300 PH range: 4.2 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: MAX IV / Beamline: BioMAX / Wavelength: 1.00003 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Apr 19, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.00003 Å / Relative weight: 1 |
Reflection | Resolution: 1.97→46.191 Å / Num. obs: 31213 / % possible obs: 100 % / Redundancy: 79.9 % / CC1/2: 1 / Net I/σ(I): 26.81 |
Reflection shell | Resolution: 1.97→2.11 Å / Num. unique obs: 5694 / CC1/2: 0.76 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1I8U Resolution: 1.97→46.191 Å / Cor.coef. Fo:Fc: 0.969 / Cor.coef. Fo:Fc free: 0.946 / WRfactor Rfree: 0.211 / WRfactor Rwork: 0.162 / SU B: 2.956 / SU ML: 0.08 / Average fsc free: 0.9379 / Average fsc overall: 0.949 / Average fsc work: 0.9496 / Cross valid method: FREE R-VALUE / ESU R: 0.104 / ESU R Free: 0.115 / Details: Hydrogens have not been used
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 46.697 Å2
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Refinement step | Cycle: LAST / Resolution: 1.97→46.191 Å
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Refine LS restraints |
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LS refinement shell |
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