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- PDB-7nmz: Structure of 14-3-3 eta in complex with Nedd4-2(335-455) containi... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7nmz | ||||||
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Title | Structure of 14-3-3 eta in complex with Nedd4-2(335-455) containing two 14-3-3 binding motifs Ser342 and Ser448 | ||||||
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Function / homology | ![]() glucocorticoid catabolic process / cerebellar granule cell to Purkinje cell synapse / positive regulation of caveolin-mediated endocytosis / RING-type E3 ubiquitin transferase (cysteine targeting) / negative regulation of sodium ion transmembrane transport / negative regulation of potassium ion transmembrane transporter activity / negative regulation of dendrite morphogenesis / regulation of potassium ion transmembrane transporter activity / negative regulation of potassium ion transmembrane transport / intracellular glucocorticoid receptor signaling pathway ...glucocorticoid catabolic process / cerebellar granule cell to Purkinje cell synapse / positive regulation of caveolin-mediated endocytosis / RING-type E3 ubiquitin transferase (cysteine targeting) / negative regulation of sodium ion transmembrane transport / negative regulation of potassium ion transmembrane transporter activity / negative regulation of dendrite morphogenesis / regulation of potassium ion transmembrane transporter activity / negative regulation of potassium ion transmembrane transport / intracellular glucocorticoid receptor signaling pathway / regulation of sodium ion transmembrane transport / negative regulation of sodium ion transmembrane transporter activity / regulation of sodium ion transmembrane transporter activity / negative regulation of protein localization to cell surface / regulation of membrane repolarization / nuclear glucocorticoid receptor binding / positive regulation of dendrite extension / potassium channel inhibitor activity / ventricular cardiac muscle cell action potential / HECT-type E3 ubiquitin transferase / membrane depolarization during action potential / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Pohl, P. / Obsil, T. / Obsilova, V. | ||||||
Funding support | ![]()
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![]() | ![]() Title: 14-3-3-protein regulates Nedd4-2 by modulating interactions between HECT and WW domains. Authors: Pohl, P. / Joshi, R. / Petrvalska, O. / Obsil, T. / Obsilova, V. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 134.5 KB | Display | ![]() |
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PDB format | ![]() | Display | ![]() | |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6zbtC ![]() 6zc9C ![]() 2c63S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 27186.777 Da / Num. of mol.: 2 / Mutation: S235Stop Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() #2: Protein | | Mass: 13442.557 Da / Num. of mol.: 1 / Mutation: T367A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: UniProt: Q96PU5, HECT-type E3 ubiquitin transferase #3: Water | ChemComp-HOH / | ![]() Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47 % |
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Crystal grow![]() | Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.03M of each NPS (sodium nitrate, sodium phosphate dibasic, ammonium sulfate), 0.1Mbicine/Trizma base pH 8.5, 30% sacharose |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: LIQUID ANODE / Type: Excillum MetalJet D2+ 70 kV / Wavelength: 1.3418 Å |
Detector | Type: Bruker PHOTON II / Detector: PIXEL / Date: Jan 20, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.303→32.69 Å / Num. obs: 32703 / % possible obs: 99.86 % / Redundancy: 4.73 % / Biso Wilson estimate: 33.19 Å2 / Rrim(I) all: 0.064 / Net I/σ(I): 19.68 |
Reflection shell | Resolution: 2.303→2.385 Å / Redundancy: 3.99 % / Mean I/σ(I) obs: 1.94 / Num. unique obs: 3215 / Rrim(I) all: 0.655 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 2C63 Resolution: 2.303→32.69 Å / SU ML: 0.1987 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 24.5299 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 43.92 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.303→32.69 Å
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Refine LS restraints |
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LS refinement shell |
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