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Yorodumi- PDB-7m1u: Crystal structure of an archaeal CNNM, MtCorB, R235L mutant with ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7m1u | |||||||||
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Title | Crystal structure of an archaeal CNNM, MtCorB, R235L mutant with C-terminal deletion | |||||||||
Components | Hemolysin, contains CBS domains | |||||||||
Keywords | MEMBRANE PROTEIN / CorB / CNNM / DUF21 / magnesium transporter | |||||||||
Function / homology | Function and homology information | |||||||||
Biological species | Methanoculleus thermophilus (archaea) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.8 Å | |||||||||
Authors | Chen, Y.S. / Kozlov, G. / Gehring, K. | |||||||||
Funding support | Canada, 1items
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Citation | Journal: Nat Commun / Year: 2021 Title: Crystal structure of an archaeal CorB magnesium transporter. Authors: Chen, Y.S. / Kozlov, G. / Moeller, B.E. / Rohaim, A. / Fakih, R. / Roux, B. / Burke, J.E. / Gehring, K. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7m1u.cif.gz | 382.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7m1u.ent.gz | 264.8 KB | Display | PDB format |
PDBx/mmJSON format | 7m1u.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m1/7m1u ftp://data.pdbj.org/pub/pdb/validation_reports/m1/7m1u | HTTPS FTP |
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-Related structure data
Related structure data | 7m1tC 7msuC 7lj6 7lj7 C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 36591.164 Da / Num. of mol.: 2 / Mutation: R235L Source method: isolated from a genetically manipulated source Source: (gene. exp.) Methanoculleus thermophilus (archaea) / Gene: SAMN04488571_101329 / Plasmid: pET29a / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / Variant (production host): C41 / References: UniProt: A0A1G8XA46 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 5.08 Å3/Da / Density % sol: 75.79 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop Details: 0.1 M sodium citrate, pH 5.5; 50 mM Li2SO4; 11% PEG4000; 10 mM MgSO4 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: CLSI / Beamline: 08B1-1 / Wavelength: 1.52154 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Feb 14, 2021 |
Radiation | Monochromator: KOHZU double crystal monochromator (DCM) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.52154 Å / Relative weight: 1 |
Reflection | Resolution: 3.8→50 Å / Num. obs: 15203 / % possible obs: 91.7 % / Redundancy: 6 % / CC1/2: 0.965 / Net I/σ(I): 16.3 |
Reflection shell | Resolution: 3.8→3.94 Å / Redundancy: 4.5 % / Mean I/σ(I) obs: 0.8 / Num. unique obs: 152 / CC1/2: 0.51 / % possible all: 78.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 7LJ6, 7LJ7 Resolution: 3.8→46.27 Å / SU ML: 0.6517 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 33.632 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 95.9 Å2 | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.8→46.27 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -12.7023134885 Å / Origin y: -30.0606052659 Å / Origin z: 23.9817900124 Å
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Refinement TLS group | Selection details: all |