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- PDB-7lny: Apo structure of the Histone chaperone ASF1A residues 1-155 -

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Basic information

Entry
Database: PDB / ID: 7lny
TitleApo structure of the Histone chaperone ASF1A residues 1-155
ComponentsHistone chaperone ASF1A
KeywordsCELL CYCLE / Histone chaperone / immunoglobulin domain-like / protein interaction / replication-coupled nucleosome assembly / replication-independent nucleosome assembly
Function / homology
Function and homology information


histone chaperone activity / muscle cell differentiation / DNA replication-dependent chromatin assembly / DNA repair-dependent chromatin remodeling / Formation of Senescence-Associated Heterochromatin Foci (SAHF) / replication fork processing / osteoblast differentiation / nucleosome assembly / site of double-strand break / histone binding ...histone chaperone activity / muscle cell differentiation / DNA replication-dependent chromatin assembly / DNA repair-dependent chromatin remodeling / Formation of Senescence-Associated Heterochromatin Foci (SAHF) / replication fork processing / osteoblast differentiation / nucleosome assembly / site of double-strand break / histone binding / DNA repair / chromatin binding / chromatin / protein-containing complex / nucleoplasm / nucleus
Similarity search - Function
Histone chaperone ASF1-like / Histone chaperone ASF1-like superfamily / ASF1 like histone chaperone
Similarity search - Domain/homology
Histone chaperone ASF1A
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.1 Å
AuthorsSimon, B. / Boggon, T.J. / Calderwood, D. / Turk, B.E.
Funding support United States, 3items
OrganizationGrant numberCountry
American Heart Association18POST33960340 United States
National Institutes of Health/National Center for Advancing Translational Sciences (NIH/NCATS)1R03TR002912-01 United States
National Institutes of Health/National Cancer Institute (NIH/NCI)P50 CA196530 United States
CitationJournal: Nat Commun / Year: 2022
Title: Tousled-like kinase 2 targets ASF1 histone chaperones through client mimicry.
Authors: Simon, B. / Lou, H.J. / Huet-Calderwood, C. / Shi, G. / Boggon, T.J. / Turk, B.E. / Calderwood, D.A.
History
DepositionFeb 8, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 16, 2022Provider: repository / Type: Initial release
Revision 1.1Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Histone chaperone ASF1A
B: Histone chaperone ASF1A
C: Histone chaperone ASF1A
D: Histone chaperone ASF1A
E: Histone chaperone ASF1A
F: Histone chaperone ASF1A
G: Histone chaperone ASF1A


Theoretical massNumber of molelcules
Total (without water)124,8097
Polymers124,8097
Non-polymers00
Water8,377465
1
A: Histone chaperone ASF1A


Theoretical massNumber of molelcules
Total (without water)17,8301
Polymers17,8301
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Histone chaperone ASF1A


Theoretical massNumber of molelcules
Total (without water)17,8301
Polymers17,8301
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: Histone chaperone ASF1A


Theoretical massNumber of molelcules
Total (without water)17,8301
Polymers17,8301
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: Histone chaperone ASF1A


Theoretical massNumber of molelcules
Total (without water)17,8301
Polymers17,8301
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
5
E: Histone chaperone ASF1A


Theoretical massNumber of molelcules
Total (without water)17,8301
Polymers17,8301
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
6
F: Histone chaperone ASF1A


Theoretical massNumber of molelcules
Total (without water)17,8301
Polymers17,8301
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
7
G: Histone chaperone ASF1A


Theoretical massNumber of molelcules
Total (without water)17,8301
Polymers17,8301
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)142.282, 118.980, 109.506
Angle α, β, γ (deg.)90.000, 103.450, 90.000
Int Tables number5
Space group name H-MC121

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Components

#1: Protein
Histone chaperone ASF1A / Anti-silencing function protein 1 homolog A / hAsf1 / hAsf1a / CCG1-interacting factor A / CIA / hCIA


Mass: 17829.896 Da / Num. of mol.: 7 / Fragment: Residues 1-155
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: ASF1A, CGI-98, HSPC146 / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta 2 / References: UniProt: Q9Y294
#2: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 465 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.65 Å3/Da / Density % sol: 66.3 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6
Details: 1 ml reservoir of 22% PEG3350 and 6% of Tascimate pH6.0

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9792 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Aug 17, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9792 Å / Relative weight: 1
ReflectionResolution: 2.1→106.5 Å / Num. obs: 102510 / % possible obs: 99.3 % / Redundancy: 4.4 % / CC1/2: 0.997 / Rmerge(I) obs: 0.061 / Rpim(I) all: 0.032 / Rrim(I) all: 0.069 / Net I/σ(I): 12.8
Reflection shell

Diffraction-ID: 1 / Redundancy: 4.5 %

Resolution (Å)Rmerge(I) obsNum. measured allNum. unique obsCC1/2Rpim(I) allRrim(I) allNet I/σ(I) obs% possible all
2.1-2.140.6982248150480.8210.3650.7912.199.4
11.5-106.50.04829906710.9960.0260.05529.399.9

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Phasing

PhasingMethod: molecular replacement
Phasing MR
Highest resolutionLowest resolution
Rotation2 Å106.5 Å
Translation2 Å106.5 Å

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Processing

Software
NameVersionClassification
PHENIX1.19refinement
XDSdata reduction
Aimless0.7.4data scaling
PHASER2.8.2phasing
PDB_EXTRACT3.27data extraction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 2I32

2i32


Resolution: 2.1→106.5 Å / SU ML: 0.31 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 27.02 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.242 10372 5.17 %0
Rwork0.2199 190215 --
obs0.221 102489 98.38 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 179.67 Å2 / Biso mean: 56.6189 Å2 / Biso min: 26.07 Å2
Refinement stepCycle: final / Resolution: 2.1→106.5 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8673 0 0 465 9138
Biso mean---60.05 -
Num. residues----1078
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0028904
X-RAY DIFFRACTIONf_angle_d0.51812179
X-RAY DIFFRACTIONf_dihedral_angle_d12.4565314
X-RAY DIFFRACTIONf_chiral_restr0.0491344
X-RAY DIFFRACTIONf_plane_restr0.0031623
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
2.1-2.12390.31453530.3559628798
2.1239-2.14890.33683750.3479632498
2.1489-2.17510.34243260.3222628198
2.1751-2.20260.33263940.3265635098
2.2026-2.23160.33093190.3104628698
2.2316-2.26220.32333020.3103629396
2.2622-2.29450.33873590.3084630199
2.2945-2.32870.33863380.3058645899
2.3287-2.36510.3183210.2989638799
2.3651-2.40390.2973140.2833631799
2.4039-2.44540.32623950.292642199
2.4454-2.48980.28343880.2867632099
2.4898-2.53770.29662890.2834636599
2.5377-2.58950.2873560.2815634198
2.5895-2.64580.30953600.2825638398
2.6458-2.70740.28383240.2942631098
2.7074-2.77510.30243230.2897622997
2.7751-2.85010.28693600.2799632599
2.8501-2.9340.28062800.2545652499
2.934-3.02870.27273790.2506634599
3.0287-3.1370.29983570.2409638399
3.137-3.26260.24633540.2357636899
3.2626-3.41110.26023220.2167636498
3.4111-3.59090.23443710.2015617896
3.5909-3.81590.20983730.1852635499
3.8159-4.11050.16983240.1756639499
4.1105-4.52420.18324120.1604634099
4.5242-5.17890.16533130.1494635798
5.1789-6.52470.20243430.1763633298
6.5247-106.50.24773480.2007629898
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.82681.31790.39663.0319-0.34961.74120.80520.48660.2064-0.99190.01-1.4232-0.25690.4350.09910.54190.1211-0.03290.60020.00440.515871.36551.94825.971
21.163-0.87310.2446-0.5061-1.53454.8710.28540.3712-0.0655-0.2824-0.36170.04990.1654-0.2787-0.0020.35280.0381-0.02260.3293-0.01370.360864.2752.17335.324
3-0.3537-0.8254-0.9473-0.01761.60092.65020.52320.2977-0.45920.2066-0.0665-0.10650.90810.29950.02810.41110.0655-0.05490.3069-0.02960.408971.64845.75244.926
41.0757-0.20280.9822-0.37340.01651.82270.40280.44950.119-0.08640.06770.14181.213-0.24240.06740.52870.0589-0.10290.4339-0.05530.321761.42548.74628.92
52.1192-1.3128-0.33020.1459-0.61271.22950.12930.1209-0.15930.0374-0.17310.0230.24540.1529-0.00620.380.0333-0.01590.2944-0.00460.356271.70749.42346.879
61.24490.30940.31870.303-0.720.71380.1834-0.08770.16580.00760.457-0.50670.14811.1792-0.00030.32990.07570.02810.4327-0.02210.341374.05954.43642.177
70.89270.3776-0.35660.3824-0.04350.25680.5316-0.64260.82090.2016-0.60370.1793-0.63010.02880.00010.5731-0.12410.06440.5241-0.08890.581969.70264.264.082
80.2496-0.5098-0.19640.37460.10143.2444-0.00570.1565-0.03670.307-0.3156-0.2753-0.7922-0.7883-0.06540.3587-0.0190.0050.2748-0.0190.421766.16760.78853.303
93.5892-1.5427-0.09332.0316-1.64631.4450.25690.3499-0.0064-0.6225-0.0587-0.5251-0.11720.61210.01590.3740.11330.02150.47060.08340.345277.61350.27731.143
106.26122.12961.24312.458-1.33392.1994-0.0168-0.56370.19590.2833-0.06280.3586-0.3035-0.35270.22440.42230.1159-0.00640.39060.00150.476886.24333.36944.847
110.2192-0.7652-0.67492.11530.29013.2529-0.0554-0.2793-0.1807-0.0021-0.2472-0.04780.24880.41960.020.35150.12110.00510.3623-0.01330.47391.60429.38234.964
124.32343.9506-2.71857.2839-2.98351.3592-0.73690.89770.7867-1.90711.05240.70380.6149-1.13240.21080.50150.18070.00990.4556-0.05780.46186.48137.75726.762
131.52150.1872-0.39851.02380.29710.9911-0.34370.62550.14910.15820.585-0.1626-0.4697-1.16080.04660.49730.184-0.02940.5408-0.09590.353385.28135.55222.781
140.6461-0.05030.1581.281-1.6981.5644-0.137-0.2171-0.1109-0.38080.08390.1940.7928-0.1205-0.00840.46050.06150.02970.408-0.00440.501687.90424.01839.211
151.2753-0.1611-0.0163-0.8905-0.15581.15570.07080.5334-0.0627-0.2342-0.1239-0.0481-0.2141-0.3206-00.58460.15440.04690.4549-0.02390.435589.49238.4524.442
160.0061-0.80740.23281.4732-0.20971.82330.03590.281-0.0001-0.2613-0.0918-0.1396-0.3913-0.0375-00.41550.09270.01180.3852-0.02620.312591.55640.95630.912
170.0731-0.2361-0.22690.24170.43070.74040.5309-0.18930.6434-0.8792-0.417-0.8862-1.18140.53840.00550.92450.03070.18080.79230.12880.8607108.7146.53414.425
180.11650.1120.00760.0595-0.0171-0.0323-0.14420.052-0.1492-0.2544-0.5263-0.03180.02320.3913-0.00010.54430.12520.06110.4850.07650.4039103.42138.91121.804
191.5879-1.59470.68672.36931.20392.42930.1643-0.66690.0148-0.1672-0.31240.2931-0.2625-0.0128-0.00010.39130.1249-0.07320.4295-0.00440.324883.63939.56340.396
201.8831-0.3735-1.13018.7793-4.57749.72460.26970.09920.3339-0.21930.23771.75870.5029-1.06070.54480.44390.1133-0.02020.515-0.02440.428945.01358.66328.431
21-0.02260.2087-0.1197-0.0277-0.04430.02070.0486-0.01260.1527-0.023-0.36490.2638-0.6120.17720.00010.7020.02210.07270.4930.09920.52660.82472.6614.687
220.8335-0.18641.85010.0507-0.18252.46110.0394-0.1534-0.3217-0.0282-0.1763-0.4020.3084-0.08570.00020.45220.0850.02730.4405-0.02620.453752.23356.19228.579
230.50040.696-1.78650.3895-0.14671.76540.1910.79470.1315-0.6950.1840.05530.6335-0.14600.49460.0903-0.01960.66030.05440.441553.17358.39310.685
240.3178-1.1643-0.08980.5567-0.0410.3891-0.10110.28380.05090.02070.0692-0.29570.1940.0174-0.02270.40940.0707-0.05280.41980.05070.395955.73757.86629.662
251.86150.1624-1.42070.45840.97541.55270.08560.45660.1758-0.41390.0448-0.0982-0.4252-0.42500.54990.09930.02190.65660.0310.412853.23662.469.441
261.9124-1.0170.50511.41181.91783.57790.20040.35040.1690.3797-0.1457-0.2596-0.7067-0.54770.00040.35740.16810.00140.47860.04560.375148.32465.5213.83
270.1259-0.1623-0.10530.08890.03990.0682-0.05420.61950.4883-0.14390.2823-0.7621-0.6677-0.4791-01.03690.08240.2270.87640.31980.821658.94782.5710.03
280.01540.09770.25670.15470.13310.41550.39730.60010.3862-0.737-0.442-0.4141-1.48570.0786-0.00060.78860.08950.22330.68690.20440.588258.78975.4379.036
293.68520.4781-0.53180.84161.40292.64210.55110.34690.00040.8998-0.52060.8262-0.0748-0.6526-0.00130.51210.0799-0.00750.5594-0.070.348741.31358.19721.318
309.16760.1188-1.22530.98190.46091.7382-0.348-1.14121.02240.55260.35620.0158-0.16650.15650.060.78350.0503-0.03110.71460.09930.470479.9750.195105.573
310.2038-0.3243-0.02190.32060.0297-0.0107-0.3801-0.9563-0.35140.5998-0.2014-1.05650.3971.059-0.00590.50680.1289-0.05670.82860.14150.7245101.69143.90392.793
320.38170.3971-0.87672.4427-1.32510.9297-0.03420.4338-0.05330.56450.31150.21530.8521-0.36360.00510.59960.0412-0.0730.77850.05720.464180.85843.169103.166
332.4708-0.0427-0.46180.40991.70542.4537-0.19020.79550.4758-0.24480.67330.1478-0.0094-0.61790.00030.41110.0469-0.03920.55180.05350.44686.52347.78287.092
34-0.06010.1604-0.3354-0.07740.00310.47620.027-0.3367-0.4393-0.1521-0.0721-0.17660.59160.8278-0.00080.61990.07770.00560.52730.15060.477483.48439.846103.925
351.1998-0.8946-0.25350.0639-0.48092.8855-0.15420.0257-0.11850.11380.0957-0.1037-0.144-0.0129-0.00010.49060.0104-0.01460.4684-0.05980.418190.62949.30386.66
36-0.12210.0322-0.32140.4977-0.4642.27640.16620.06470.42010.11320.0897-0.2138-0.26690.21040.00010.41040.0058-0.04630.5099-0.07390.42991.16753.51292.747
370.34910.3902-0.0770.5553-0.01-0.0313-0.1217-0.5809-0.00110.4546-0.2532-0.5351-0.24761.1131-0.00020.549-0.021-0.08681.0015-0.03540.7094112.72252.37781.308
380.6808-1.0966-1.13422.32981.91171.7907-0.1227-1.86810.02-0.39910.5529-0.9135-0.98092.3520.13990.42410.1282-0.01980.92290.03590.6059104.68848.09988.506
390.7336-1.84770.15733.80210.12582.46460.0421-0.89190.83010.01990.09270.037-0.2696-0.1236-0.00430.57290.0441-0.04610.5551-0.05190.280480.70155.58199.244
402.4242-0.0957-1.90551.7989-0.28335.35870.4408-0.073-0.29740.23150.0762-0.58720.1968-0.33170.26930.51060.15860.00340.268-0.05030.6469102.92214.92848.475
410.7441-0.28650.59291.0291-0.73010.39730.04360.7839-0.1518-0.3035-0.14170.04390.7547-0.3008-0.01280.48240.10620.01370.5861-0.07040.562597.09816.34639.61
421.1722-1.5604-1.12981.01020.98321.5361-0.1917-0.6116-0.24860.50990.53020.22870.2899-1.5050.01480.50610.04570.05610.54880.06310.539993.00211.21956.376
430.9447-0.0394-0.23971.377-0.86510.35930.20490.4076-0.0146-0.1274-0.0704-0.00230.32060.29070.00010.46370.14120.03440.459-0.01230.463598.40820.56140.512
442.2275-0.725-1.59311.60210.42130.7671-0.1244-0.6135-0.44720.39630.15460.1997-0.1335-0.2196-00.6270.10130.03850.55230.05640.572396.25710.89859.253
451.35760.2149-1.12011.6847-1.55741.4107-0.2961-0.9349-0.19060.15830.0438-0.0686-0.6855-0.7249-0.00340.47560.1283-0.13030.3948-0.05410.526101.6948.2355.012
460.2116-0.33270.1290.1574-0.09130.0529-0.7637-1.77270.51051.30060.7785-1.0477-0.94770.38510.00051.35210.2122-0.29551.3439-0.03370.9192110.82813.56277.039
470.2060.0767-0.3126-0.0886-0.04880.4497-0.3135-1.9710.02010.70230.6516-0.5054-0.59361.29810.0140.78550.1657-0.13770.8205-0.10030.5715107.1416.52766.308
483.19620.43751.62861.5963-0.89151.49450.5421.8043-1.5391-0.27890.3993-0.11620.47990.97270.05170.53910.1393-0.09360.557-0.16820.671598.534.44543.545
490.30560.39960.3360.54740.31220.51760.20010.2739-0.1702-0.4151-0.1312-0.8019-0.06190.60490.00020.51940.11270.13430.61780.05360.7689112.21931.99142.771
500.76220.3806-1.93550.98740.63582.3127-0.03610.0650.2205-0.13960.1868-0.65070.35630.3752-0.00230.43090.10480.00280.50390.02430.5867106.81130.78750.836
511.6356-0.2353-1.1359-0.16290.59061.5746-0.4379-0.52310.43990.36640.795-0.9103-0.21911.3690.00360.50330.1019-0.09780.6926-0.02520.6451111.539.47160.895
520.6293-0.18320.11671.7251.30381.2395-0.0685-0.2673-0.22550.13380.3312-0.54060.22320.66350.01360.44680.0990.01690.56230.00930.6162108.83735.18455.899
530.0270.712-0.14961.94970.65250.8278-0.0443-0.143-0.1499-0.08420.1787-0.5994-0.42810.35430.02810.3314-0.0180.04050.5223-0.00080.579107.83643.08952.957
541.5052-0.093-0.44730.3156-0.26620.7079-0.06360.20020.26290.3829-0.03550.2238-0.12410.0554-00.43340.014-0.01310.42540.01580.406188.84951.00364.602
550.3120.22410.96722.12690.22062.54340.25380.2599-0.2309-0.5002-0.1455-0.6637-0.27630.55140.00120.49080.03820.11930.488-0.00250.6266111.6740.16548.232
562.57660.35280.77571.57882.42999.5002-0.52350.02950.1349-0.16960.2382-0.3481-1.39561.67340.46290.61950.1181-0.06180.28740.11070.5891108.62127.5373.246
570.53250.87780.39790.7192-0.32010.796-0.38370.0809-0.5525-0.6028-0.1497-0.4196-0.85190.94350.00750.65010.05430.00690.35190.04580.6148107.13529.738-3.421
581.7815-0.0263-0.85040.6595-1.51092.1944-0.37250.62190.0098-0.93860.86640.94381.2876-2.10690.06120.7697-0.1524-0.07570.6657-0.02190.680598.89524.634-8.379
592.14480.7208-0.40680.77280.23350.1709-0.32470.4156-0.2968-1.0490.33370.92151.246-1.1011-0.00950.80280.02720.11240.61070.0030.883798.51818.9532.403
60-0.22790.4194-0.29520.3164-0.33920.01730.064-0.05330.0877-0.03950.21380.211-0.385-0.3134-00.5740.0449-0.08750.47910.07760.5303104.63932.609-4.172
610.9207-0.2443-0.83872.45410.63511.9944-0.94170.0881-0.0389-0.181-0.21170.18290.8913-0.9245-0.14660.68890.02910.28640.4696-0.01180.6732101.77717.9877.777
621.26291.354-0.80471.8435-1.36682.4768-1.1122-0.5686-0.06-0.6331-0.4240.3505-0.4885-1.238-0.22270.66270.13030.14180.4910.0180.5936108.36418.3134.476
630.3466-0.1469-0.13760.9479-0.95791.266-0.9082-1.3372-0.66520.3661-0.0499-0.9947-0.43630.7401-0.03690.91710.33810.2731.15070.23520.9143110.89319.42929.116
641.12820.5714-0.19870.32070.54522.7113-0.19020.0833-0.4642-0.2257-0.9344-0.4842-1.88370.7575-0.11730.62180.15570.05770.52320.20590.6633111.78723.27113.552
650.6288-0.1952-0.4076-0.07390.11410.2143-0.07030.6278-1.0526-0.2325-0.01210.74850.02880.7732-0.00021.0065-0.05490.21460.6615-0.11970.8497106.29715.615-10.815
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND RESID 2:13 )A2 - 13
2X-RAY DIFFRACTION2( CHAIN A AND RESID 14:39 )A14 - 39
3X-RAY DIFFRACTION3( CHAIN A AND RESID 40:63 )A40 - 63
4X-RAY DIFFRACTION4( CHAIN A AND RESID 64:78 )A64 - 78
5X-RAY DIFFRACTION5( CHAIN A AND RESID 79:105 )A79 - 105
6X-RAY DIFFRACTION6( CHAIN A AND RESID 106:119 )A106 - 119
7X-RAY DIFFRACTION7( CHAIN A AND RESID 120:133 )A120 - 133
8X-RAY DIFFRACTION8( CHAIN A AND RESID 134:141 )A134 - 141
9X-RAY DIFFRACTION9( CHAIN A AND RESID 142:156 )A142 - 156
10X-RAY DIFFRACTION10( CHAIN B AND RESID 2:13 )B2 - 13
11X-RAY DIFFRACTION11( CHAIN B AND RESID 14:39 )B14 - 39
12X-RAY DIFFRACTION12( CHAIN B AND RESID 40:52 )B40 - 52
13X-RAY DIFFRACTION13( CHAIN B AND RESID 53:63 )B53 - 63
14X-RAY DIFFRACTION14( CHAIN B AND RESID 64:78 )B64 - 78
15X-RAY DIFFRACTION15( CHAIN B AND RESID 79:105 )B79 - 105
16X-RAY DIFFRACTION16( CHAIN B AND RESID 106:119 )B106 - 119
17X-RAY DIFFRACTION17( CHAIN B AND RESID 120:133 )B120 - 133
18X-RAY DIFFRACTION18( CHAIN B AND RESID 134:141 )B134 - 141
19X-RAY DIFFRACTION19( CHAIN B AND RESID 142:156 )B142 - 156
20X-RAY DIFFRACTION20( CHAIN C AND RESID 3:13 )C3 - 13
21X-RAY DIFFRACTION21( CHAIN C AND RESID 14:23 )C14 - 23
22X-RAY DIFFRACTION22( CHAIN C AND RESID 24:39 )C24 - 39
23X-RAY DIFFRACTION23( CHAIN C AND RESID 40:64 )C40 - 64
24X-RAY DIFFRACTION24( CHAIN C AND RESID 65:78 )C65 - 78
25X-RAY DIFFRACTION25( CHAIN C AND RESID 79:105 )C79 - 105
26X-RAY DIFFRACTION26( CHAIN C AND RESID 106:119 )C106 - 119
27X-RAY DIFFRACTION27( CHAIN C AND RESID 120:133 )C120 - 133
28X-RAY DIFFRACTION28( CHAIN C AND RESID 134:141 )C134 - 141
29X-RAY DIFFRACTION29( CHAIN C AND RESID 142:156 )C142 - 156
30X-RAY DIFFRACTION30( CHAIN D AND RESID 2:13 )D2 - 13
31X-RAY DIFFRACTION31( CHAIN D AND RESID 14:23 )D14 - 23
32X-RAY DIFFRACTION32( CHAIN D AND RESID 24:39 )D24 - 39
33X-RAY DIFFRACTION33( CHAIN D AND RESID 40:64 )D40 - 64
34X-RAY DIFFRACTION34( CHAIN D AND RESID 65:78 )D65 - 78
35X-RAY DIFFRACTION35( CHAIN D AND RESID 79:105 )D79 - 105
36X-RAY DIFFRACTION36( CHAIN D AND RESID 106:119 )D106 - 119
37X-RAY DIFFRACTION37( CHAIN D AND RESID 120:133 )D120 - 133
38X-RAY DIFFRACTION38( CHAIN D AND RESID 134:141 )D134 - 141
39X-RAY DIFFRACTION39( CHAIN D AND RESID 142:156 )D142 - 156
40X-RAY DIFFRACTION40( CHAIN E AND RESID 3:23 )E3 - 23
41X-RAY DIFFRACTION41( CHAIN E AND RESID 24:39 )E24 - 39
42X-RAY DIFFRACTION42( CHAIN E AND RESID 40:64 )E40 - 64
43X-RAY DIFFRACTION43( CHAIN E AND RESID 65:78 )E65 - 78
44X-RAY DIFFRACTION44( CHAIN E AND RESID 79:105 )E79 - 105
45X-RAY DIFFRACTION45( CHAIN E AND RESID 106:119 )E106 - 119
46X-RAY DIFFRACTION46( CHAIN E AND RESID 120:133 )E120 - 133
47X-RAY DIFFRACTION47( CHAIN E AND RESID 134:141 )E134 - 141
48X-RAY DIFFRACTION48( CHAIN E AND RESID 142:156 )E142 - 156
49X-RAY DIFFRACTION49( CHAIN F AND RESID 4:13 )F4 - 13
50X-RAY DIFFRACTION50( CHAIN F AND RESID 14:39 )F14 - 39
51X-RAY DIFFRACTION51( CHAIN F AND RESID 40:63 )F40 - 63
52X-RAY DIFFRACTION52( CHAIN F AND RESID 64:105 )F64 - 105
53X-RAY DIFFRACTION53( CHAIN F AND RESID 106:119 )F106 - 119
54X-RAY DIFFRACTION54( CHAIN F AND RESID 120:136 )F120 - 136
55X-RAY DIFFRACTION55( CHAIN F AND RESID 137:156 )F137 - 156
56X-RAY DIFFRACTION56( CHAIN G AND RESID 4:23 )G4 - 23
57X-RAY DIFFRACTION57( CHAIN G AND RESID 24:32 )G24 - 32
58X-RAY DIFFRACTION58( CHAIN G AND RESID 33:46 )G33 - 46
59X-RAY DIFFRACTION59( CHAIN G AND RESID 47:69 )G47 - 69
60X-RAY DIFFRACTION60( CHAIN G AND RESID 70:78 )G70 - 78
61X-RAY DIFFRACTION61( CHAIN G AND RESID 79:105 )G79 - 105
62X-RAY DIFFRACTION62( CHAIN G AND RESID 106:119 )G106 - 119
63X-RAY DIFFRACTION63( CHAIN G AND RESID 120:133 )G120 - 133
64X-RAY DIFFRACTION64( CHAIN G AND RESID 134:146 )G134 - 146
65X-RAY DIFFRACTION65( CHAIN G AND RESID 147:155 )G147 - 155

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