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- PDB-7kua: Crystal structure of the MarR family transcriptional regulator fr... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7kua | ||||||
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Title | Crystal structure of the MarR family transcriptional regulator from Pseudomonas putida bound to Indole 3 acetic acid | ||||||
![]() | Transcriptional regulator, MarR family![]() | ||||||
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Function / homology | MarR family / MarR-type HTH domain profile. / helix_turn_helix multiple antibiotic resistance protein / MarR-type HTH domain / Winged helix DNA-binding domain superfamily / DNA-binding transcription factor activity / Winged helix-like DNA-binding domain superfamily / 1H-INDOL-3-YLACETIC ACID / IacR![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Walton, W.G. / Lietzan, A.D. / Redinbo, M.R. / Dangl, J.L. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Diverse MarR bacterial regulators of auxin catabolism in the plant microbiome. Authors: Conway, J.M. / Walton, W.G. / Salas-Gonzalez, I. / Law, T.F. / Lindberg, C.A. / Crook, L.E. / Kosina, S.M. / Fitzpatrick, C.R. / Lietzan, A.D. / Northen, T.R. / Jones, C.D. / Finkel, O.M. / ...Authors: Conway, J.M. / Walton, W.G. / Salas-Gonzalez, I. / Law, T.F. / Lindberg, C.A. / Crook, L.E. / Kosina, S.M. / Fitzpatrick, C.R. / Lietzan, A.D. / Northen, T.R. / Jones, C.D. / Finkel, O.M. / Redinbo, M.R. / Dangl, J.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 53.4 KB | Display | ![]() |
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PDB format | ![]() | 32.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7kfoC ![]() 7kfqC ![]() 7kfsC ![]() 7kh3C ![]() 7kigC ![]() 7kjlC ![]() 7kjqC ![]() 7kk0C ![]() 7kkcC ![]() 7kkiC ![]() 7krhC ![]() 7kymC ![]() 7l19C ![]() 7l1iC ![]() 3cjnS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 22120.930 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-IAC / ![]() |
#3: Water | ChemComp-HOH / ![]() |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.98 Å3/Da / Density % sol: 37.73 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: Anatrace MCSG2 screen 35 % (v/v) Microlytic Mix(1), pH 7.0, which consists of: 1.8305 M Malonic acid, 0.25 M Ammonium citrate tribasic, 0.12 M Succinic acid, 0.3 M DL-Malic acid, 0.4 M ...Details: Anatrace MCSG2 screen 35 % (v/v) Microlytic Mix(1), pH 7.0, which consists of: 1.8305 M Malonic acid, 0.25 M Ammonium citrate tribasic, 0.12 M Succinic acid, 0.3 M DL-Malic acid, 0.4 M Sodium acetate trihydrate, 0.5 M Sodium formate, 0.16 M Ammonium tartrate dibasic, the mixture titrated to pH 7.0 using sodium hydroxide |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Nov 15, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.88→50 Å / Num. obs: 14591 / % possible obs: 99.9 % / Redundancy: 8.3 % / Biso Wilson estimate: 29.48 Å2 / CC1/2: 1 / Net I/σ(I): 33.4 |
Reflection shell | Resolution: 1.88→1.91 Å / Num. unique obs: 715 / CC1/2: 0.957 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 3CJN Resolution: 1.89→25.99 Å / SU ML: 0.2014 / Cross valid method: FREE R-VALUE / σ(F): 1.38 / Phase error: 26.0325 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 39.08 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.89→25.99 Å
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Refine LS restraints |
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LS refinement shell |
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