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- PDB-7ewo: Crystal Structure of D67A, E68P double mutant of O-acetyl-L-serin... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7ewo | ||||||
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Title | Crystal Structure of D67A, E68P double mutant of O-acetyl-L-serine sulfhydrylase from Haemophilus influenzae | ||||||
![]() | Cysteine synthase![]() | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Rahisuddin, R. / Ekka, M.K. / Singh, A.K. / Saini, N. / Patel, M. / Kumar, N. / Kumaran, S. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal Structure of D67A, E68P double mutant of O-acetyl-L-serine sulfhydrylase from Haemophilus influenzae Authors: Rahisuddin, R. / Ekka, M.K. / Singh, A.K. / Saini, N. / Patel, M. / Kumaran, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 147.4 KB | Display | ![]() |
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PDB format | ![]() | 93 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4ho1S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 37244.453 Da / Num. of mol.: 1 / Mutation: D67E, A68P Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: cysK, HI_1103 / Plasmid: pET28a Production host: ![]() ![]() ![]() References: UniProt: P45040, ![]() |
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#2: Water | ChemComp-HOH / ![]() |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.87 Å3/Da / Density % sol: 34.34 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.5 / Details: 0.1M HEPES, 1.3M Sodium Citrate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Sep 26, 2012 |
Radiation | Monochromator: M / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.397→40.59 Å / Num. obs: 10586 / % possible obs: 95.93 % / Redundancy: 5.6 % / Biso Wilson estimate: 43.7 Å2 / Rmerge(I) obs: 0.2 / Net I/σ(I): 31.8 |
Reflection shell | Resolution: 2.397→2.483 Å / Redundancy: 4.1 % / Rmerge(I) obs: 0.23 / Mean I/σ(I) obs: 2.8 / Num. unique obs: 786 / % possible all: 73.94 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 4HO1 Resolution: 2.4→40.59 Å / SU ML: 0.2549 / Cross valid method: FREE R-VALUE / σ(F): 1.37 / Phase error: 26.0497 / Stereochemistry target values: GeoStd + Monomer Library
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 50.68 Å2 | ||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.4→40.59 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 15.0865181773 Å / Origin y: 3.72909906416 Å / Origin z: -4.49140423341 Å
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Refinement TLS group | Selection details: all |