[English] 日本語
Yorodumi- PDB-7cg1: Solution structure of the sensor domain of the anti-sigma factor ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7cg1 | ||||||
---|---|---|---|---|---|---|---|
Title | Solution structure of the sensor domain of the anti-sigma factor RsgI4 in Pseudobacteroides cellulosolvens | ||||||
Components | Anti-sigma factor RsgI, N-terminal | ||||||
Keywords | TRANSCRIPTION / Sugar binding protein | ||||||
Function / homology | Anti-sigma factor RsgI, N-terminal / Anti-sigma factor N-terminus / RsgI N-terminal anti-sigma domain profile. / membrane / Anti-sigma factor RsgI, N-terminal Function and homology information | ||||||
Biological species | Pseudobacteroides cellulosolvens ATCC 35603 = DSM 2933 (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Dong, S. / Feng, Y. | ||||||
Funding support | China, 1items
| ||||||
Citation | Journal: To Be Published Title: Structure of the sensor domain of the anti-sigma factor RsgI4 in Pseudobacteroides cellulosolvens Authors: Dong, S. / Chen, C. / Feng, Y. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 7cg1.cif.gz | 792.6 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb7cg1.ent.gz | 668.2 KB | Display | PDB format |
PDBx/mmJSON format | 7cg1.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cg/7cg1 ftp://data.pdbj.org/pub/pdb/validation_reports/cg/7cg1 | HTTPS FTP |
---|
-Related structure data
Related structure data | 7cg5C 7cg8C C: citing same article (ref.) |
---|---|
Similar structure data | |
Other databases |
|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 14526.462 Da / Num. of mol.: 1 / Fragment: sensor domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudobacteroides cellulosolvens ATCC 35603 = DSM 2933 (bacteria) Gene: Bccel_2225 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: A0A0L6JMH4 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details | Type: solution Contents: 1 mM [U-13C; U-15N] the sensor domain of RsgI4, 50 mM sodium acetate, 0.02 % w/v sodium azide, 0.01 % w/v DSS, 90% H2O/10% D2O Label: sample1 / Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample |
| ||||||||||||||||||||
Sample conditions | Ionic strength: 50 mM / Label: condition1 / pH: 5 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 600 MHz |
---|
-Processing
NMR software |
| ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing / Software ordinal: 5 | ||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |