[English] 日本語
![](img/lk-miru.gif)
- PDB-7acf: CRYSTAL STRUCTURE OF CRYSTAL FORM 2 OF AN ACTIVE KRAS G12D (GPPCP... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 7acf | ||||||
---|---|---|---|---|---|---|---|
Title | CRYSTAL STRUCTURE OF CRYSTAL FORM 2 OF AN ACTIVE KRAS G12D (GPPCP) DIMER IN COMPLEX WITH BI-5747 | ||||||
![]() | GTPase KRas | ||||||
![]() | ![]() ![]() | ||||||
Function / homology | ![]() forebrain astrocyte development / negative regulation of epithelial cell differentiation / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kessler, D. | ||||||
![]() | ![]() Title: Reply to Tran et al.: Dimeric KRAS protein-protein interaction stabilizers. Authors: Kessler, D. / Gollner, A. / Gmachl, M. / Mantoulidis, A. / Martin, L.J. / Zoephel, A. / Mayer, M. / Covini, D. / Fischer, S. / Gerstberger, T. / Gmaschitz, T. / Goodwin, C. / Greb, P. / ...Authors: Kessler, D. / Gollner, A. / Gmachl, M. / Mantoulidis, A. / Martin, L.J. / Zoephel, A. / Mayer, M. / Covini, D. / Fischer, S. / Gerstberger, T. / Gmaschitz, T. / Goodwin, C. / Greb, P. / Haering, D. / Hela, W. / Hoffmann, J. / Karolyi-Oezguer, J. / Knesl, P. / Kornigg, S. / Koegl, M. / Kousek, R. / Lamarre, L. / Moser, F. / Munico-Martinez, S. / Peinsipp, C. / Phan, J. / Rinnenthal, J. / Sai, J. / Salamon, C. / Scherbantin, Y. / Schipany, K. / Schnitzer, R. / Schrenk, A. / Sharps, B. / Siszler, G. / Sun, Q. / Waterson, A. / Wolkerstorfer, B. / Zeeb, M. / Pearson, M. / Fesik, S.W. / McConnell, D.B. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 295.3 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 239.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 6quuS S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 19386.848 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-MG / #3: Chemical | ChemComp-GCP / #4: Chemical | #5: Water | ChemComp-HOH / | ![]() Has ligand of interest | Y | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.66 Å3/Da / Density % sol: 53.76 % |
---|---|
Crystal grow![]() | Temperature: 278 K / Method: vapor diffusion / Details: Morpheus Screen E9 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Feb 18, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.9→40 Å / Num. obs: 64227 / % possible obs: 100 % / Redundancy: 10.2 % / Biso Wilson estimate: 32.89 Å2 / CC1/2: 1 / Rmerge(I) obs: 0.083 / Rrim(I) all: 0.09 / Net I/σ(I): 17.5 |
Reflection shell | Resolution: 1.907→1.914 Å / Num. unique obs: 4695 / CC1/2: 0.7 |
-
Processing
Software |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure![]() ![]() Starting model: 6QUU Resolution: 1.91→40 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.943 / Rfactor Rfree error: 0 / SU R Cruickshank DPI: 0.138 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.143 / SU Rfree Blow DPI: 0.117 / SU Rfree Cruickshank DPI: 0.116
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 40.26 Å2
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.24 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.91→40 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 1.91→1.96 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement TLS group |
|