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Open data
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Basic information
Entry | Database: PDB / ID: 6zvo | ||||||
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Title | Crystal structure of unliganded MLKL executioner domain | ||||||
![]() | Mixed lineage kinase domain-like protein | ||||||
![]() | LIPID BINDING PROTEIN / ![]() | ||||||
Function / homology | ![]() execution phase of necroptosis / Microbial modulation of RIPK1-mediated regulated necrosis / necroptotic signaling pathway / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fiegen, D. / Bauer, M. / Nar, H. | ||||||
![]() | ![]() Title: Locking mixed-lineage kinase domain-like protein in its auto-inhibited state prevents necroptosis. Authors: Rubbelke, M. / Fiegen, D. / Bauer, M. / Binder, F. / Hamilton, J. / King, J. / Thamm, S. / Nar, H. / Zeeb, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 208.8 KB | Display | ![]() |
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PDB format | ![]() | 169 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6zleC ![]() 6zprC ![]() 6zz1C ![]() 4btfS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 17642.326 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() #2: Chemical | ![]() #3: Chemical | ChemComp-BR / | ![]() #4: Water | ChemComp-HOH / | ![]() Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.99 Å3/Da / Density % sol: 38.12 % |
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Crystal grow![]() | Temperature: 277 K / Method: vapor diffusion, hanging drop Details: 31 % Polyethylene glycol monomethyl ether 2,000, 0.15 M Potassium bromide, 0.1 M TRIS, pH 9 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jun 8, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.375→70.158 Å / Num. obs: 65990 / % possible obs: 94.4 % / Redundancy: 6.5 % / CC1/2: 0.999 / Net I/σ(I): 18.3 |
Reflection shell | Resolution: 1.375→1.483 Å / Num. unique obs: 3300 / CC1/2: 0.518 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 4btf Resolution: 1.371→70.16 Å / Cor.coef. Fo:Fc: 0.962 / Cor.coef. Fo:Fc free: 0.945 / SU R Cruickshank DPI: 0.082 / Cross valid method: THROUGHOUT / SU R Blow DPI: 0.087 / SU Rfree Blow DPI: 0.091 / SU Rfree Cruickshank DPI: 0.088
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Displacement parameters | Biso mean: 27.1 Å2
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Refine analyze | Luzzati coordinate error obs: 0.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.371→70.16 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.371→1.45 Å
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