分子量: 53089.223 Da / 分子数: 1 変異: S83G,A86L,T101R,H103D,H105Y,L119F,M121L,E124D,R143K,D150E,A161V,R167L,R213L,V234L,K235R,V240L,I253A,I260A,N262R,K263R,H305R,C332V,F342A,T354S,F358V,S362A 由来タイプ: 組換発現 詳細: Residues 58-371 represent the rat neurotensin receptor 1 mutant H4 (NTSR1-H4). Residues 273 to 290 were deleted for crystallisation purposes. Residues 372 to 380 form a linker connecting ...詳細: Residues 58-371 represent the rat neurotensin receptor 1 mutant H4 (NTSR1-H4). Residues 273 to 290 were deleted for crystallisation purposes. Residues 372 to 380 form a linker connecting NTSR1 with the DARPin crystallisation chaperone. Residues 381 to 536 represent the DARPin crystallisation chaperone that is related to 5LW2. Residues 537 to 539 represent a short linker connecting the DARPin crystallisation chaperone with the HRV 3C protease recognition sequence. Residues 540 to 544 are part of the HRV 3C protease recognition sequence visible in the electron density. 由来: (組換発現) Rattus norvegicus (ドブネズミ), (組換発現) synthetic construct (人工物) 遺伝子: Ntsr1, Ntsr / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P20789
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 3.78 Å3/Da / 溶媒含有率: 67.5 %
結晶化
温度: 293.1 K / 手法: 脂質キュービック相法 / pH: 4.5 詳細: 100 mM Na acetate 400-475 mM K citrate 30-31% (v/v) PEG400