+Open data
-Basic information
Entry | Database: PDB / ID: 6v8f | ||||||
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Title | Crystal structure of recombinat mutant Q185R of human fumarase | ||||||
Components | Fumarate hydratase, mitochondrialFumarase | ||||||
Keywords | LYASE / HsFH / fumarate hydratase | ||||||
Function / homology | Function and homology information regulation of arginine metabolic process / tricarboxylic acid cycle heteromeric enzyme complex / fumarate hydratase activity / fumarate hydratase / fumarate metabolic process / Citric acid cycle (TCA cycle) / malate metabolic process / urea cycle / positive regulation of double-strand break repair via nonhomologous end joining / homeostasis of number of cells within a tissue ...regulation of arginine metabolic process / tricarboxylic acid cycle heteromeric enzyme complex / fumarate hydratase activity / fumarate hydratase / fumarate metabolic process / Citric acid cycle (TCA cycle) / malate metabolic process / urea cycle / positive regulation of double-strand break repair via nonhomologous end joining / homeostasis of number of cells within a tissue / tricarboxylic acid cycle / site of double-strand break / chromosome / positive regulation of cold-induced thermogenesis / histone binding / mitochondrial matrix / DNA repair / DNA damage response / mitochondrion / extracellular exosome / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å | ||||||
Authors | Ajalla, M.A.A. / Nonato, M.C. | ||||||
Funding support | Brazil, 1items
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Citation | Journal: To Be Published Title: Crystal structure of recombinat mutant Q185R of human fumarase Authors: Ajalla, M.A.A. / Nonato, M.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6v8f.cif.gz | 202.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6v8f.ent.gz | 158.8 KB | Display | PDB format |
PDBx/mmJSON format | 6v8f.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/v8/6v8f ftp://data.pdbj.org/pub/pdb/validation_reports/v8/6v8f | HTTPS FTP |
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-Related structure data
Related structure data | 5uppS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 50168.457 Da / Num. of mol.: 2 / Mutation: Q185R Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: FH / Plasmid: pET28a-SUMO / Details (production host): pET28a modified vector / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P07954, fumarate hydratase #2: Chemical | #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.88 Å3/Da / Density % sol: 57.3 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 0.1 M HEPES, 0.2 M Lithium sulfate, 25% (m/v) PEG 3350 PH range: 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 1 / Wavelength: 0.97857 Å |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Feb 9, 2018 |
Radiation | Monochromator: channel cut cryogenically cooled monochromator Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97857 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→95.244 Å / Num. obs: 54953 / % possible obs: 100 % / Redundancy: 32.4 % / CC1/2: 0.999 / Net I/σ(I): 19.6 |
Reflection shell | Resolution: 2.3→2.37 Å / Num. unique obs: 4425 / CC1/2: 0.927 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5UPP Resolution: 2.3→95.24 Å / SU ML: 0.19 / Cross valid method: FREE R-VALUE / σ(F): 1.46 / Phase error: 14.98
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 122.65 Å2 / Biso mean: 34.1849 Å2 / Biso min: 7.83 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.3→95.24 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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