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- PDB-6uzi: Crystal structure of Dihydrolipoyl dehydrogenase from Elizabethki... -

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Basic information

Entry
Database: PDB / ID: 6uzi
TitleCrystal structure of Dihydrolipoyl dehydrogenase from Elizabethkingia anophelis NUHP1
ComponentsDihydrolipoyl dehydrogenaseDihydrolipoamide dehydrogenase
KeywordsOXIDOREDUCTASE / SSGCID / Structural Genomics / Dihydrolipoyl dehydrogenase / BD94_2540 / Seattle Structural Genomics Center for Infectious Disease
Function / homology
Function and homology information


dihydrolipoyl dehydrogenase / dihydrolipoyl dehydrogenase activity / cell redox homeostasis / flavin adenine dinucleotide binding
Similarity search - Function
Dihydrolipoamide dehydrogenase / Pyridine nucleotide-disulphide oxidoreductase, class I / FAD/NAD-linked reductase, C-terminal dimerisation domain / Pyridine nucleotide-disulphide oxidoreductase, class I, active site / Pyridine nucleotide-disulphide oxidoreductases class-I active site. / Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain / Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain / FAD/NAD-linked reductase, dimerisation domain superfamily / FAD/NAD(P)-binding domain / Pyridine nucleotide-disulphide oxidoreductase ...Dihydrolipoamide dehydrogenase / Pyridine nucleotide-disulphide oxidoreductase, class I / FAD/NAD-linked reductase, C-terminal dimerisation domain / Pyridine nucleotide-disulphide oxidoreductase, class I, active site / Pyridine nucleotide-disulphide oxidoreductases class-I active site. / Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain / Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain / FAD/NAD-linked reductase, dimerisation domain superfamily / FAD/NAD(P)-binding domain / Pyridine nucleotide-disulphide oxidoreductase / Enolase-like; domain 1 / FAD/NAD(P)-binding domain / FAD/NAD(P)-binding domain / 3-Layer(bba) Sandwich / FAD/NAD(P)-binding domain superfamily / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
FLAVIN-ADENINE DINUCLEOTIDE / Dihydrolipoyl dehydrogenase
Similarity search - Component
Biological speciesElizabethkingia anophelis NUHP1 (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å
AuthorsSeattle Structural Genomics Center for Infectious Disease (SSGCID)
CitationJournal: TO BE PUBLISHED
Title: Crystal structure of Dihydrolipoyl dehydrogenase from Elizabethkingia anophelis NUHP1
Authors: Abendroth, J. / Dranow, D.M. / Lorimer, D.D. / Horanyi, P.S. / Edwards, T.E.
History
DepositionNov 15, 2019Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 15, 2020Provider: repository / Type: Initial release
Revision 1.1Oct 11, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_conn / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Dihydrolipoyl dehydrogenase
B: Dihydrolipoyl dehydrogenase
C: Dihydrolipoyl dehydrogenase
D: Dihydrolipoyl dehydrogenase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)208,43321
Polymers204,5504
Non-polymers3,88317
Water3,981221
1
A: Dihydrolipoyl dehydrogenase
B: Dihydrolipoyl dehydrogenase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)104,35813
Polymers102,2752
Non-polymers2,08311
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area11930 Å2
ΔGint-140 kcal/mol
Surface area32740 Å2
MethodPISA
2
C: Dihydrolipoyl dehydrogenase
D: Dihydrolipoyl dehydrogenase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)104,0748
Polymers102,2752
Non-polymers1,7996
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area11450 Å2
ΔGint-134 kcal/mol
Surface area32610 Å2
MethodPISA
Unit cell
Length a, b, c (Å)74.060, 106.800, 255.700
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 2 or (resid 3 and (name...
21(chain B and (resid 2 through 33 or (resid 34...
31(chain C and (resid 2 through 33 or (resid 34...
41(chain D and (resid 2 through 106 or (resid 107...

NCS domain segments:

Ens-ID: 1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11ASNASNASNASN(chain A and (resid 2 or (resid 3 and (name...AA210
12GLNGLNGLNGLN(chain A and (resid 2 or (resid 3 and (name...AA311
13METMETFADFAD(chain A and (resid 2 or (resid 3 and (name...AA - E1 - 5019
14METMETFADFAD(chain A and (resid 2 or (resid 3 and (name...AA - E1 - 5019
15METMETFADFAD(chain A and (resid 2 or (resid 3 and (name...AA - E1 - 5019
16METMETFADFAD(chain A and (resid 2 or (resid 3 and (name...AA - E1 - 5019
21ASNASNGLUGLU(chain B and (resid 2 through 33 or (resid 34...BB2 - 3310 - 41
22LYSLYSLYSLYS(chain B and (resid 2 through 33 or (resid 34...BB3442
23METMETFADFAD(chain B and (resid 2 through 33 or (resid 34...BB - M1 - 5019
31ASNASNGLUGLU(chain C and (resid 2 through 33 or (resid 34...CC2 - 3310 - 41
32LYSLYSLYSLYS(chain C and (resid 2 through 33 or (resid 34...CC3442
33HISHISFADFAD(chain C and (resid 2 through 33 or (resid 34...CC - P-2 - 5016
34HISHISFADFAD(chain C and (resid 2 through 33 or (resid 34...CC - P-2 - 5016
35HISHISFADFAD(chain C and (resid 2 through 33 or (resid 34...CC - P-2 - 5016
36HISHISFADFAD(chain C and (resid 2 through 33 or (resid 34...CC - P-2 - 5016
41ASNASNASNASN(chain D and (resid 2 through 106 or (resid 107...DD2 - 10610 - 114
42LYSLYSLYSLYS(chain D and (resid 2 through 106 or (resid 107...DD107115
43ASNASNFADFAD(chain D and (resid 2 through 106 or (resid 107...DD - U2 - 50110
44ASNASNFADFAD(chain D and (resid 2 through 106 or (resid 107...DD - U2 - 50110
45ASNASNFADFAD(chain D and (resid 2 through 106 or (resid 107...DD - U2 - 50110
46ASNASNFADFAD(chain D and (resid 2 through 106 or (resid 107...DD - U2 - 50110

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Components

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Protein , 1 types, 4 molecules ABCD

#1: Protein
Dihydrolipoyl dehydrogenase / Dihydrolipoamide dehydrogenase


Mass: 51137.500 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Elizabethkingia anophelis NUHP1 (bacteria)
Gene: BD94_2540 / Plasmid: ElanA.01412.a.B1
Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria)
Strain (production host): BL21(DE3)
References: UniProt: A0A077ELH4, dihydrolipoyl dehydrogenase

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Non-polymers , 5 types, 238 molecules

#2: Chemical
ChemComp-FAD / FLAVIN-ADENINE DINUCLEOTIDE / Flavin adenine dinucleotide


Mass: 785.550 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C27H33N9O15P2 / Feature type: SUBJECT OF INVESTIGATION / Comment: FAD*YM
#3: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Zn
#4: Chemical ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Cl
#5: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol


Mass: 62.068 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C2H6O2
#6: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 221 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.48 Å3/Da / Density % sol: 50.3 %
Crystal growTemperature: 290 K / Method: vapor diffusion, sitting drop / pH: 6.5
Details: Rigaku Reagents JCSG+ screen, condition B2: 20% w/V PEG 3350, 200mM sodium thiocyanate: ElanA.01412.a.B1.PS38371 at 19.1 mg/ml. Cryo: 25% EG: tray 296479b2: puck CEB1-4.

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 21-ID-F / Wavelength: 0.9787 Å
DetectorType: RAYONIX MX-300 / Detector: CCD / Date: Dec 7, 2017
RadiationMonochromator: C(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9787 Å / Relative weight: 1
ReflectionResolution: 2.8→45.25 Å / Num. obs: 50364 / % possible obs: 98.9 % / Redundancy: 6.23 % / Biso Wilson estimate: 53.341 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.088 / Rrim(I) all: 0.096 / Χ2: 1.022 / Net I/σ(I): 17 / Num. measured all: 313757 / Scaling rejects: 24
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured obsNum. possibleNum. unique obsCC1/2Rrim(I) all% possible all
2.8-2.876.2840.632.8423221370136950.8560.68799.8
2.87-2.956.3030.5333.3822919364136360.8740.58199.9
2.95-3.046.3220.4394.1322012349334820.9180.47899.7
3.04-3.136.3020.3535.1921439342234020.9480.38599.4
3.13-3.236.2810.2726.7320873335033230.9650.29699.2
3.23-3.356.2940.2039.0420033320531830.9770.22299.3
3.35-3.476.2920.15711.3519234308630570.9860.17199.1
3.47-3.616.2710.12613.818563299829600.990.13798.7
3.61-3.786.2730.10117.0817822287628410.9950.1198.8
3.78-3.966.2160.08220.2416957276627280.9950.0998.6
3.96-4.176.2910.0723.5216217261925780.9960.07798.4
4.17-4.436.2110.05628.0615253249424560.9980.06198.5
4.43-4.736.2390.04831.6814387234023060.9980.05298.5
4.73-5.116.1930.04731.5713384220021610.9980.05198.2
5.11-5.66.150.05229.1612417204220190.9980.05698.9
5.6-6.266.1540.05229.4611213185018220.9980.05698.5
6.26-7.236.0930.04432.369858164616180.9990.04898.3
7.23-8.856.0350.0341.448304140413760.9990.03298
8.85-12.525.8460.02746.036425112210990.9990.02998
12.52-45.255.1860.02844.4632266686220.9980.03193.1

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Processing

Software
NameVersionClassification
XDSdata reduction
XSCALEdata scaling
PHENIX1.17.1 -3660refinement
PDB_EXTRACT3.25data extraction
MoRDaphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB entry 3urh as per Morda

3urh


Resolution: 2.8→45.25 Å / SU ML: 0.36 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 23.1
RfactorNum. reflection% reflectionSelection details
Rfree0.2295 1947 3.87 %0
Rwork0.1689 ---
obs0.1713 50342 98.9 %-
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 134.11 Å2 / Biso mean: 58.2917 Å2 / Biso min: 21.17 Å2
Refinement stepCycle: final / Resolution: 2.8→45.25 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13658 0 243 221 14122
Biso mean--65.22 42.33 -
Num. residues----1870
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00414100
X-RAY DIFFRACTIONf_angle_d0.72219167
X-RAY DIFFRACTIONf_dihedral_angle_d19.6915127
X-RAY DIFFRACTIONf_chiral_restr0.052303
X-RAY DIFFRACTIONf_plane_restr0.0042594
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A8446X-RAY DIFFRACTION8.239TORSIONAL
12B8446X-RAY DIFFRACTION8.239TORSIONAL
13C8446X-RAY DIFFRACTION8.239TORSIONAL
14D8446X-RAY DIFFRACTION8.239TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 14

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.8-2.870.33221640.235634253589100
2.87-2.950.29871350.230834363571100
2.95-3.030.27831440.213934103554100
3.03-3.130.28571400.20633434357499
3.13-3.240.27061490.19623420356999
3.24-3.370.27631270.20143454358199
3.37-3.530.26051430.19513419356299
3.53-3.710.21981340.17033428356299
3.71-3.950.22851440.15883420356499
3.95-4.250.20951330.14833423355698
4.25-4.680.16841180.12483485360398
4.68-5.350.19311290.13673489361898
5.35-6.740.21891480.17493515366399
6.74-45.250.20981390.16373637377697
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
18.99681.6943.90347.8361.7721.94-0.07490.5475-0.2548-0.69660.18470.01960.06980.2141-0.06310.4762-0.03260.02770.2326-0.0010.3354-9.61229.4421-71.7922
24.2681.05323.35342.23211.18256.2292-0.29490.039-0.0089-0.4016-0.01340.1901-0.1022-0.4020.28940.4216-0.027-0.02030.2984-0.0010.54878.442322.6202-54.6674
30.9830.14280.41271.1195-0.05080.6064-0.0330.1779-0.0894-0.25470.00510.0262-0.11640.08990.05820.446-0.01820.03890.32980.00690.35710.982522.8066-64.4577
42.1364-0.3754-0.33021.8437-0.48371.47610.028-0.03690.20980.0848-0.0749-0.0007-0.23970.0210.0250.38460.0001-0.02780.2249-0.01210.31270.536434.7973-43.9883
53.9634-0.51553.18761.13840.22225.7868-0.0345-0.26780.00650.00920.01960.3405-0.0201-0.51560.02980.2577-0.00230.04310.2920.00980.3665-16.865314.2166-59.24
63.04880.4668-0.65552.30460.31492.37350.0243-0.3547-0.27430.4085-0.0609-0.02280.19620.02990.01820.3255-0.0066-0.03960.28720.07180.2765-1.05967.6084-33.4506
70.0361-0.02640.29551.1067-0.77592.7401-0.3659-0.7293-0.36810.31520.1707-0.2410.49450.39680.16660.74060.0946-0.16140.72090.25770.601814.6676-4.6078-16.4899
81.6824-0.14760.35291.1973-0.08251.06290.0478-0.13030.06590.3122-0.1739-0.4747-0.05760.62520.11840.3577-0.0128-0.11960.48250.01680.559523.716613.2495-35.744
91.3495-0.29110.02620.80160.05340.5338-0.0175-0.5721-0.35970.50050.0467-0.56110.54760.77810.03760.73410.1777-0.27240.70070.12430.749427.975-5.3073-25.9602
103.2251-0.9211-0.32320.28810.12681.65680.04880.2563-0.7183-0.0402-0.1059-0.96790.56140.6579-0.04110.47130.175-0.05220.50830.02540.844529.3334-5.4938-49.8454
111.5927-0.5592-0.30341.13910.07361.9306-0.2076-0.427-0.51990.40.2236-0.48240.77050.59040.06740.86130.3042-0.17150.63140.15480.943326.0456-14.6437-31.15
122.3409-0.3007-2.21691.1007-0.86586.7361-0.2397-0.3164-0.45430.04770.1561-0.05541.07310.00750.02270.69260.0448-0.0670.37230.11460.49956.1818-10.5738-29.2219
131.4956-0.00310.21630.50650.81131.97170.03560.0536-0.21350.0639-0.016-0.14270.32380.0103-0.02460.36520.0304-0.01980.20560.03310.39385.1708-2.3214-52.2677
142.3205-0.0509-1.22280.4761-1.16474.76850.15080.00560.60720.69620.0394-0.2127-1.03990.1989-0.15950.88070.14890.02780.37050.02910.7151-22.084281.7364-31.5797
154.41233.31-0.31743.7306-0.09742.14770.2260.565-0.13120.54290.1527-0.4947-0.4897-0.4199-0.39180.50730.2067-0.00310.61420.00560.4645-37.181958.6556-37.8469
161.24530.2757-0.27691.21320.34561.617-0.06430.17050.1803-0.04750.02690.0569-0.2585-0.29180.02780.4360.1612-0.01980.43170.03830.3451-30.03357.3161-39.8179
173.4501-1.2744-0.83792.93650.36812.45940.12250.3144-0.0359-0.427-0.1892-0.2155-0.0783-0.58130.0070.48140.04810.00090.4283-0.03050.3409-21.767742.9431-48.7427
184.52191.5536-0.30522.3988-0.16745.4616-0.0703-0.14420.1679-0.09010.3337-0.6564-0.60410.2063-0.28210.55310.06580.04410.378-0.05060.6891-10.40769.129-32.1667
193.75391.8137-0.70093.64510.14713.2979-0.3062-0.55740.51230.41730.234-0.2934-0.67080.03460.04730.67130.1697-0.05710.4412-0.04810.5236-20.751767.489-20.8237
202.20540.35290.1481.86230.7484.7147-0.0883-0.1697-0.11020.2030.0338-0.21060.2686-0.21180.07070.48670.14430.04480.40250.0530.4013-31.960347.0621-13.257
217.49752.3026-2.25353.1677-0.00791.89370.13620.1367-0.19540.0616-0.18940.05040.1486-1.0187-0.04090.5949-0.01890.10840.82870.05250.4662-47.040935.54954.7495
224.97550.20320.23884.23070.56851.60610.4565-0.2342-0.09330.583-0.40610.3341-0.2981-0.9042-0.12670.49150.0060.01211.0311-0.03270.4211-56.621546.5659-18.4511
231.65350.12550.26080.23320.13660.83830.0119-0.2931-0.2410.1320.03720.23620.1621-1.0414-0.03760.56070.01740.10761.11310.00380.518-60.308443.2434-10.7573
241.1225-0.49520.09250.23850.00890.4891-0.1241-0.34940.44250.2888-0.16460.1874-0.2333-1.02990.02240.65990.43150.05361.2716-0.07080.6252-64.164765.1288-15.5057
254.32810.03910.34061.42450.77242.7833-0.2196-0.21710.2111-0.0459-0.25890.3541-0.1344-1.09920.40340.56720.26350.10071.4751-0.06570.6564-74.569359.7443-12.3928
262.41730.2076-1.9832.5534-1.89624.0817-0.2299-0.5369-0.31210.46550.09030.10320.0699-0.75310.24670.75740.19490.11891.15740.01220.4249-55.911148.115610.9971
271.30430.3639-0.23761.4427-0.76681.74610.1766-0.4086-0.11640.36410.0898-0.0987-0.3831-0.3112-0.03360.5680.13390.03670.73960.00960.3693-42.940550.78333.0866
281.41030.3929-0.53180.529-0.02041.5791-0.1277-0.0380.37460.20630.1290.0717-0.5701-0.78130.02140.70380.36910.0850.6296-0.00790.5359-45.200868.2533-14.7105
291.82860.1947-0.01060.8832-0.26291.7132-0.08860.10190.38890.14870.0568-0.0041-0.8034-0.37640.0030.66740.24680.02130.5192-0.03910.4427-36.834767.4686-14.6353
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 33 )A1 - 33
2X-RAY DIFFRACTION2chain 'A' and (resid 34 through 65 )A34 - 65
3X-RAY DIFFRACTION3chain 'A' and (resid 66 through 163 )A66 - 163
4X-RAY DIFFRACTION4chain 'A' and (resid 164 through 275 )A164 - 275
5X-RAY DIFFRACTION5chain 'A' and (resid 276 through 351 )A276 - 351
6X-RAY DIFFRACTION6chain 'A' and (resid 352 through 467 )A352 - 467
7X-RAY DIFFRACTION7chain 'B' and (resid 1 through 46 )B1 - 46
8X-RAY DIFFRACTION8chain 'B' and (resid 47 through 105 )B47 - 105
9X-RAY DIFFRACTION9chain 'B' and (resid 106 through 195 )B106 - 195
10X-RAY DIFFRACTION10chain 'B' and (resid 196 through 244 )B196 - 244
11X-RAY DIFFRACTION11chain 'B' and (resid 245 through 320 )B245 - 320
12X-RAY DIFFRACTION12chain 'B' and (resid 321 through 351 )B321 - 351
13X-RAY DIFFRACTION13chain 'B' and (resid 352 through 467 )B352 - 467
14X-RAY DIFFRACTION14chain 'C' and (resid -2 through 33 )C-2 - 33
15X-RAY DIFFRACTION15chain 'C' and (resid 34 through 65 )C34 - 65
16X-RAY DIFFRACTION16chain 'C' and (resid 66 through 236 )C66 - 236
17X-RAY DIFFRACTION17chain 'C' and (resid 237 through 275 )C237 - 275
18X-RAY DIFFRACTION18chain 'C' and (resid 276 through 320 )C276 - 320
19X-RAY DIFFRACTION19chain 'C' and (resid 321 through 351 )C321 - 351
20X-RAY DIFFRACTION20chain 'C' and (resid 352 through 467 )C352 - 467
21X-RAY DIFFRACTION21chain 'D' and (resid 2 through 33 )D2 - 33
22X-RAY DIFFRACTION22chain 'D' and (resid 34 through 65 )D34 - 65
23X-RAY DIFFRACTION23chain 'D' and (resid 66 through 195 )D66 - 195
24X-RAY DIFFRACTION24chain 'D' and (resid 196 through 244 )D196 - 244
25X-RAY DIFFRACTION25chain 'D' and (resid 245 through 275 )D245 - 275
26X-RAY DIFFRACTION26chain 'D' and (resid 276 through 320 )D276 - 320
27X-RAY DIFFRACTION27chain 'D' and (resid 321 through 351 )D321 - 351
28X-RAY DIFFRACTION28chain 'D' and (resid 352 through 390 )D352 - 390
29X-RAY DIFFRACTION29chain 'D' and (resid 391 through 467 )D391 - 467

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