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- PDB-6tbl: Crystal structure of MMS19(CTD)-CIAO1-CIAO2B CIA targeting complex -

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Basic information

Entry
Database: PDB / ID: 6tbl
TitleCrystal structure of MMS19(CTD)-CIAO1-CIAO2B CIA targeting complex
Components
  • MIP18 family protein galla-2
  • MMS19 nucleotide excision repair protein homolog
  • Probable cytosolic iron-sulfur protein assembly protein Ciao1
KeywordsBIOSYNTHETIC PROTEIN / Fe-S cluster / Fe-S / CIA / CIA targeting complex
Function / homology
Function and homology information


cytosolic [4Fe-4S] assembly targeting complex / protein maturation by [4Fe-4S] cluster transfer / protein maturation by iron-sulfur cluster transfer / MMXD complex / iron-sulfur cluster assembly / DNA metabolic process / positive regulation of double-strand break repair via homologous recombination / chromosome segregation / nuclear estrogen receptor binding / spindle ...cytosolic [4Fe-4S] assembly targeting complex / protein maturation by [4Fe-4S] cluster transfer / protein maturation by iron-sulfur cluster transfer / MMXD complex / iron-sulfur cluster assembly / DNA metabolic process / positive regulation of double-strand break repair via homologous recombination / chromosome segregation / nuclear estrogen receptor binding / spindle / transcription coactivator activity / cell division / DNA repair / DNA damage response / positive regulation of DNA-templated transcription / enzyme binding / nucleoplasm / nucleus / cytosol / cytoplasm
Similarity search - Function
Probable cytosolic iron-sulfur protein assembly protein, CIAO1/Cia1 / MMS19, C-terminal / MMS19, N-terminal / MIP18 family / DNA repair/transcription protein MET18/MMS19 / RNAPII transcription regulator C-terminal / Dos2-interacting transcription regulator of RNA-Pol-II / MIP18 family-like / Iron-sulfur cluster assembly protein / Fe-S cluster assembly domain superfamily ...Probable cytosolic iron-sulfur protein assembly protein, CIAO1/Cia1 / MMS19, C-terminal / MMS19, N-terminal / MIP18 family / DNA repair/transcription protein MET18/MMS19 / RNAPII transcription regulator C-terminal / Dos2-interacting transcription regulator of RNA-Pol-II / MIP18 family-like / Iron-sulfur cluster assembly protein / Fe-S cluster assembly domain superfamily / Armadillo-like helical / Armadillo-type fold / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
: / Probable cytosolic iron-sulfur protein assembly protein Ciao1 / MMS19 nucleotide excision repair protein homolog / MIP18 family protein galla-2
Similarity search - Component
Biological speciesMus musculus (house mouse)
Drosophila melanogaster (fruit fly)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.65 Å
AuthorsKassube, S.A. / Thoma, N.H.
Funding support Switzerland, 3items
OrganizationGrant numberCountry
European Molecular Biology OrganizationALTF 871-2014 Switzerland
Swiss National Science FoundationCRSII3_160734 Switzerland
European Research Council666068 Switzerland
CitationJournal: Nat Struct Mol Biol / Year: 2020
Title: Structural insights into Fe-S protein biogenesis by the CIA targeting complex.
Authors: Susanne A Kassube / Nicolas H Thomä /
Abstract: The cytosolic iron-sulfur (Fe-S) assembly (CIA) pathway is required for the insertion of Fe-S clusters into cytosolic and nuclear client proteins, including many DNA replication and repair factors. ...The cytosolic iron-sulfur (Fe-S) assembly (CIA) pathway is required for the insertion of Fe-S clusters into cytosolic and nuclear client proteins, including many DNA replication and repair factors. The molecular mechanisms of client protein recognition and Fe-S cluster transfer remain unknown. Here, we report crystal structures of the CIA targeting complex (CTC), revealing that its CIAO2B subunit is centrally located and bridges CIAO1 and the client adaptor protein MMS19. Cryo-EM reconstructions of human CTC bound either to the DNA replication factor primase or to the DNA helicase DNA2, combined with biochemical, biophysical and yeast complementation assays, reveal an evolutionarily conserved, bipartite client recognition mode facilitated by CIAO1 and the structural flexibility of the MMS19 subunit. Unexpectedly, the primase Fe-S cluster is located ~70 Å away from the CTC reactive cysteine, implicating conformational dynamics of the CTC or additional maturation factors in the mechanism of Fe-S cluster transfer.
History
DepositionNov 1, 2019Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jul 29, 2020Provider: repository / Type: Initial release
Revision 1.1Feb 10, 2021Group: Database references / Category: citation
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2May 1, 2024Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: MMS19 nucleotide excision repair protein homolog
B: MMS19 nucleotide excision repair protein homolog
C: MIP18 family protein galla-2
D: MIP18 family protein galla-2
E: Probable cytosolic iron-sulfur protein assembly protein Ciao1
F: Probable cytosolic iron-sulfur protein assembly protein Ciao1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)137,58311
Polymers137,3196
Non-polymers2645
Water18010
1
A: MMS19 nucleotide excision repair protein homolog
C: MIP18 family protein galla-2
E: Probable cytosolic iron-sulfur protein assembly protein Ciao1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)68,8236
Polymers68,6593
Non-polymers1633
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: MMS19 nucleotide excision repair protein homolog
D: MIP18 family protein galla-2
F: Probable cytosolic iron-sulfur protein assembly protein Ciao1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)68,7615
Polymers68,6593
Non-polymers1012
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)144.070, 144.070, 166.430
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number90
Space group name H-MP4212
Space group name HallP4ab2ab
Symmetry operation#1: x,y,z
#2: -y+1/2,x+1/2,z
#3: y+1/2,-x+1/2,z
#4: x+1/2,-y+1/2,-z
#5: -x+1/2,y+1/2,-z
#6: -x,-y,z
#7: y,x,-z
#8: -y,-x,-z

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Components

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Protein , 3 types, 6 molecules ABCDEF

#1: Protein MMS19 nucleotide excision repair protein homolog / MET18 homolog / MMS19-like protein


Mass: 13570.036 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Mms19, Mms19l / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q9D071
#2: Protein MIP18 family protein galla-2


Mass: 17806.127 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: galla-2, CG7949 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q9VTC4
#3: Protein Probable cytosolic iron-sulfur protein assembly protein Ciao1


Mass: 37283.277 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: Ciao1, CG12797 / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q7K1Y4

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Non-polymers , 3 types, 15 molecules

#4: Chemical ChemComp-K / POTASSIUM ION


Mass: 39.098 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: K
#5: Chemical ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol


Mass: 62.068 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C2H6O2
#6: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 10 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.22 Å3/Da / Density % sol: 61.76 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / Details: 0.2 M KSCN, 20% PEG 3350

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Data collection

DiffractionMean temperature: 292 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X06DA / Wavelength: 0.999995 Å
DetectorType: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Apr 15, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.999995 Å / Relative weight: 1
ReflectionResolution: 2.65→19.99 Å / Num. obs: 51309 / % possible obs: 99.64 % / Redundancy: 53.1 % / Biso Wilson estimate: 81.78 Å2 / CC1/2: 1 / Rmerge(I) obs: 0.2125 / Rpim(I) all: 0.02927 / Rrim(I) all: 0.2146 / Net I/σ(I): 23.53
Reflection shellResolution: 2.65→2.744 Å / Redundancy: 50.4 % / Mean I/σ(I) obs: 0.83 / Num. unique obs: 5018 / CC1/2: 0.363 / % possible all: 99.98

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Processing

Software
NameVersionClassification
PHENIX1.16_3549refinement
XDSdata reduction
XDSdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: CIAO1-CIAO2B structure, to be deposited

Resolution: 2.65→19.99 Å / SU ML: 0.4329 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 28.9381
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2422 2566 5 %
Rwork0.1977 48721 -
obs0.1999 51287 99.96 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 102.97 Å2
Refinement stepCycle: LAST / Resolution: 2.65→19.99 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms9425 0 14 10 9449
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00239668
X-RAY DIFFRACTIONf_angle_d0.497613164
X-RAY DIFFRACTIONf_chiral_restr0.0441522
X-RAY DIFFRACTIONf_plane_restr0.00341684
X-RAY DIFFRACTIONf_dihedral_angle_d13.07555826
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.65-2.70.41021390.36822630X-RAY DIFFRACTION99.96
2.7-2.760.3481410.33272678X-RAY DIFFRACTION100
2.76-2.820.33821400.32252668X-RAY DIFFRACTION99.96
2.82-2.880.38681400.30542655X-RAY DIFFRACTION99.89
2.88-2.950.33321410.3032687X-RAY DIFFRACTION99.96
2.95-3.030.37671400.28412662X-RAY DIFFRACTION99.96
3.03-3.120.32171410.2872673X-RAY DIFFRACTION99.93
3.12-3.220.31241400.27762654X-RAY DIFFRACTION99.89
3.22-3.340.31121430.26112713X-RAY DIFFRACTION99.96
3.34-3.470.31581410.24442674X-RAY DIFFRACTION99.96
3.47-3.630.29391420.23012700X-RAY DIFFRACTION99.93
3.63-3.820.23921420.20712700X-RAY DIFFRACTION100
3.82-4.050.23161420.18122706X-RAY DIFFRACTION100
4.05-4.360.20621440.16732732X-RAY DIFFRACTION100
4.36-4.80.17911440.15082719X-RAY DIFFRACTION100
4.8-5.480.2381450.15982762X-RAY DIFFRACTION100
5.48-6.860.2061470.18692794X-RAY DIFFRACTION100
6.86-19.990.19321540.15112914X-RAY DIFFRACTION99.77
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.29359385702-1.758785482120.5095978989384.74323852302-0.01202218508852.280441009950.075107984799-0.295162712875-0.487199320612-0.124082963216-0.4279252976150.4472067324760.221068071189-0.699864951263-0.1867342338280.72365512177-0.108511593555-0.004571600621241.28729207424-0.1399146150960.727640029162454.860826552140.12422305514.2417040286
24.65357455877-1.824520028160.6124644223482.597103269460.6409648010873.248808946080.0186037874427-0.03109088142910.0381127036620.01075431588-0.04120489964960.09353461818640.406361813578-0.955465153625-0.1252233390010.645569674599-0.06404968331460.002610189328431.12697319429-0.06478431038370.726821042772458.582805075142.0975389822.7014525775
35.13145282484-3.31559954058-0.2225425954244.979949518381.17148019060.723055718273-0.176553458633-1.18576397328-0.2172659646310.7972412038740.1147214729020.5645833295480.211258199681-1.23130029325-0.1179917242350.654858390178-0.1077468164190.01107074585191.27296949782-0.04658925496910.728900833267459.812347608143.86059350333.5513458242
43.81923601708-1.451665922940.1768354602172.182496581630.3833517786093.295729737630.1909556721560.2006990540840.2694716925380.00226992588464-0.174970512698-0.1939517276070.146347956613-0.824845447892-0.08324632436960.614442028574-0.0239531985750.01589372269230.942195083025-0.04140635006360.740246313899469.292152803144.09499336729.3450248018
53.51898365343-1.12654377224-0.2657102778781.33967887334-0.9663511899111.58071016295-0.7751822394040.1009966896-1.47684479714-0.3070835609920.766919788214-0.8923905051451.325054345791.36787427649-0.1982103791941.42486414210.2616459534770.1890407832770.920298668480.03824052627860.939953700978445.172152379162.357036271-6.83091776612
60.503705160979-0.4333510498770.3036086800272.4819832782-0.03055743302862.31791196707-0.3406159215250.3283458828960.874691682903-0.2430947084440.3296925463730.186613916720.7852733118970.381360146543-0.03001786844691.149416670610.2367463212730.1269640348810.738399209753-0.005815013319850.637417585754439.222548748171.314795472-6.0953433063
72.285892731260.04821344037550.02003436080574.77175776798-0.5663825568093.6448511491-0.2270176799050.213888154083-0.171452246943-0.07393083247280.1990633961580.1867754153870.8181632686630.676589709446-0.01165765006651.016044230860.1887338311660.1377934552670.777113737620.01349125180460.681659858885439.594488848171.0212139742.40262702176
83.997416408173.00550359776-0.8847489989536.24968744564-0.5293188560580.995848953898-0.27210571805-0.443729635355-0.2122968401391.151460581640.124676658658-0.2944632595880.6355843890360.332547744821-0.01630801909691.066799516710.1485512067820.1645140612510.743127429120.09679545840840.761569397116437.732480289171.88110319113.199832395
93.458614361961.79729768805-0.3239915731854.48296571546-1.428562290152.44622196884-0.134490364209-0.31966892655-0.03679831421380.2300669244140.2143895581260.215373613570.3031443421720.641216175090.08185022943930.8360263719620.1600870577390.04904636330650.7846540675798.02221987991E-50.711604703996439.94655086183.2317124077.60834731487
105.090402222823.31642844793-3.226160902983.79150498618-2.442352593912.107807299010.346827258788-0.305307956203-1.10935713229-0.1113968114160.3733491093892.954028999241.01137696626-1.96244998334-0.01051251025121.07741305128-0.0001907084635460.01073302088640.9060379227670.1626680623541.37364195818423.785957784174.20775872314.2754763064
110.5666008078841.614270201271.308141857765.713278626194.854428931744.09900848758-0.237689355457-1.457915645440.2361542296261.0917078208-0.3116165807540.604696898714-0.333730480294-0.5536505161250.4169889439961.13133230420.03787737861630.1575318681161.06016182564-0.1257848971331.16645548715487.887906519119.16339827652.3633225114
127.54954968864-0.3196660168183.450972542354.63607563847-1.58873897242.02222592513-0.4188678617050.8185564780470.259831750887-0.758775208917-0.183353040995-0.0411342788802-0.755715504305-0.3050472381970.05812476999131.23058927065-0.161629722750.09180156436421.33780817911-0.1581306382041.39314645252478.740213429116.26040527939.0480421387
131.88835684084-0.683373828154-2.081330559640.6520513018420.6349239550792.84764843850.465958134980.760722287082-0.751584594411-1.709944612260.0234624414969-0.999413916021-0.826593921072-0.6238029977220.02478830611740.7588305377110.0927168036456-0.1003347469281.02795911577-0.1802266107340.897304659152477.914230683127.33430163227.1034990076
141.658303474880.5373964746080.3854970796582.743111393970.6334319912592.20102911598-0.1431039380810.3162328286640.101191932088-0.2362670769090.0838414529326-0.07456539947760.1274848533180.023338704757-0.003600261365610.6121772047370.01214582856150.02749738842570.7404781467270.03392394772150.751034529567486.355715339148.44556685536.3343532493
150.462868482950.0846917917778-0.1753498678441.719636633740.2817039951331.298386715830.112186075478-0.413137580028-0.145209949440.361988510664-0.216922273317-0.0349386308453-0.0196271893744-0.373948224469-0.02761635421630.585459486958-0.04293405392550.02717368654120.739949683737-0.02806591147570.681246536379479.562953984148.39621590445.8093172148
162.03976509255-0.07762612901462.510386589221.6996289647-1.667301062114.400388240480.2735473377491.289290854160.370845014276-0.823764829386-0.5802564032350.420173387488-0.598604850003-1.25878332775-0.04199091165140.7734231958250.0791139284391-0.101040188140.93903851955-0.04452825852281.0144246373472.692395779158.55482742836.6898931643
172.627163602930.8945396483750.651211680252.50975750398-0.758398222690.561067931702-0.1348960928850.698637495907-0.858664092476-0.0906712083829-0.0886299106925-0.207079715051.47474972966-0.2037863680370.1929960762021.20065685787-0.04939039461630.1614285547840.97519834063-0.06422413357670.940246367943460.673415944198.30050436325.2411929652
181.10483485967-0.2996696004381.220800386333.187680792520.09206024372421.897750291850.08499368385521.051158979730.271739847042-0.419375523516-0.0870216644435-0.1631274678550.0347152151980.06164268882170.03668183313450.7147727331750.03886941938360.04230371576650.7942078088910.02878463638370.739308316103434.277756422196.33421627511.5104071734
191.86928561599-0.7600942736190.5683546905630.742679045449-0.5260858257552.49130218673-0.105719908077-0.3991321314470.09586816754580.2656895474650.1164182605650.0624216711482-0.0599681159795-0.05430320989360.08860053985070.649836599160.00532218663120.03097945933740.665666820701-0.03020807071990.786718050816431.0461706193.11581573224.6053517554
203.054687908-0.7339302333051.918041771462.694877882622.053390238693.64269562316-0.8089870566090.485342718524-1.08069411387-0.5452180206390.4266937209030.05750279240371.0865693484-0.597771975887-0.03483230942930.708156519341-0.06681484225340.08244959891030.822366176101-0.117209158910.975080158216421.818794532182.94151595416.6689950226
211.549899937160.06454163680150.2213131203391.87969867217-0.0807349577250.8356137808330.0265948617277-0.1622021943130.06072888791750.953909276765-0.1831805679440.7945634907680.122699486442-1.01414543578-0.06490678838141.04384813324-0.2794051276620.3219330054161.40961001124-0.2900618029791.17128864672457.823472371133.57987899956.7936416125
221.573444732910.8612709299330.5584370662392.63717144993-0.4194551722611.225522484240.198473613428-0.06797116528210.3588761034320.344317788159-0.2822791762981.27997693692-0.0256464928933-1.07188752507-0.1981803957050.907036624361-0.1643114346490.3330341653341.47766258784-0.3602853598121.31314162676452.954944374143.08665111357.9337276928
230.6941400240670.4713800668120.8238521808470.750150876570.4441831302581.073973317130.225471479904-1.171339137370.09094215140780.2241996133530.1708992210820.427735299310.328714396189-1.67652329147-0.2114485271651.14783143364-0.2214249072230.3335274382922.09668465175-0.2725187207591.58550539023451.967515705151.52674208461.3913188592
241.5587433269-1.170693466810.0633305360612.8606725177-0.8173728739141.382794878380.109144616442-0.252732480176-0.1519797530150.715594974724-0.1445842716241.16633820196-1.20404326163-0.430774716677-0.09400492718141.205493284290.08777561216580.2963771600011.58671380393-0.2806439959561.29074200624459.891364336155.55016483363.3018881192
251.09725694557-1.137693055451.078685514711.48446534084-1.282889429391.057508886240.0580122433685-1.031086359860.660149891970.891549339819-0.3503996651730.527527276140.397943244718-0.481947879077-0.3024548255271.28137108984-0.2843829859070.5278269946921.35808521789-0.5748178702571.16922263113463.901087796154.16902573870.7093249444
261.85924481184-0.751060987077-0.456994869262.484049454890.6138756627841.772102075520.207325111749-0.8412589545860.4606673936771.18274681566-0.3814086831630.604368938429-0.0546766138882-0.404639097361-0.04841282782981.42726343247-0.388101861630.2499389254341.47260086879-0.2757948545011.03462004988470.605996656151.42602405773.5224651184
270.670750164889-0.4838526507821.151837454222.25859955366-2.387143652083.260514035730.34861794146-0.9740331399940.1742190067441.35879427628-0.5516341441610.6423570645880.718087779576-0.126376765281-0.09813703290451.62943632356-0.3911156700510.1568728011941.39264285943-0.09890331911970.859515457427475.303563732137.5881267271.1614549494
282.03097180891-0.5318301863270.2374432301331.86996044133-0.3858564708331.052488514140.21798304596-0.933122455896-0.09369818568111.18514444692-0.2341744253530.170702703571.166853224920.0853760402149-0.165250926061.41623496385-0.351712631130.1130460876761.277516992070.03825956232810.961330904463475.858738339134.88142214170.763844461
291.88930972209-0.6474309474880.2518654254981.58772336833-0.229256137791.271416889110.0586732551253-0.308173363636-0.8411184454131.15010758232-0.3182431690230.2909503252730.739322917173-0.447898926868-0.2088827294631.36970497906-0.368944935210.2163510528931.2615707464-0.05052404468271.1274919796467.611204084128.66224731862.2899534074
302.07028226758-1.492452726810.2190431412962.05045687635-0.818755448492.151133151490.284136236427-0.0268563121826-0.780676105346-0.1495402482490.0463675103774-0.05477254822160.4398767992230.4269811052110.08876829650050.7763946980630.1357838189380.09577163790210.913942865960.1131162194450.996903286353449.861408312172.93654008237.4270549615
311.72446574749-0.3124340696170.5528162223221.122723889770.5650477023221.417858020080.173592869662-0.357430782837-0.4806084963160.2524006429970.2150009654510.2796853781980.6051330267510.0438882280416-0.07397205954250.899717851940.107658341449-0.02228036789270.8204817688080.1358901056891.02902683274437.915618832166.25117739239.0254053766
320.6409534572190.4266609001620.2770249708961.358788882130.6100048549921.93690323220.0880312949346-0.720076755816-1.262882681550.3436760994850.2998611257880.642148441320.906926645464-0.302350384027-0.1962361422521.1014536099-0.04682669010490.04303724880831.028092584980.2798416102491.19184101126429.829659921168.82623465346.3897080144
331.701519362840.2637021349090.0538719999782.61962481689-0.7132986006391.610967761450.0100001830846-0.842893122449-0.2293116600890.7505838665960.1464034142240.259925209771-0.254718951157-0.03277734209620.06802816201390.8724093221960.07962704624040.108465189351.132527503910.02201047454280.818040778645434.35477024186.00604210453.2270544837
342.42977008949-0.6673600962660.2738313368832.92854290587-1.01815211691.106023496560.0109279566708-0.4547501005090.2167048058250.2477915181770.011991132914-0.525216928626-0.08613757989750.6691150553920.1282096473230.733913942501-0.0726489120655-0.0204251978821.13802456677-0.04886158916580.951554301519451.699094997187.0457975244.4330666853
351.98832389921-1.638211734450.5851909785062.07518394222-1.326895543511.87723914860.106861239785-0.746726849014-0.141829513536-0.04517458122520.0446695738035-0.367004905331-0.06451677884970.3776284388230.02575552109940.6975429082830.0517970238982-0.004019230647481.045340832140.1029375686090.863609095951451.188199502175.94968110739.9139605361
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 913 through 945 )
2X-RAY DIFFRACTION2chain 'A' and (resid 946 through 986 )
3X-RAY DIFFRACTION3chain 'A' and (resid 987 through 1006 )
4X-RAY DIFFRACTION4chain 'A' and (resid 1007 through 1031 )
5X-RAY DIFFRACTION5chain 'B' and (resid 912 through 927 )
6X-RAY DIFFRACTION6chain 'B' and (resid 928 through 945 )
7X-RAY DIFFRACTION7chain 'B' and (resid 946 through 986 )
8X-RAY DIFFRACTION8chain 'B' and (resid 987 through 1006 )
9X-RAY DIFFRACTION9chain 'B' and (resid 1007 through 1024 )
10X-RAY DIFFRACTION10chain 'B' and (resid 1025 through 1031 )
11X-RAY DIFFRACTION11chain 'C' and (resid 0 through 9 )
12X-RAY DIFFRACTION12chain 'C' and (resid 10 through 20 )
13X-RAY DIFFRACTION13chain 'C' and (resid 21 through 35 )
14X-RAY DIFFRACTION14chain 'C' and (resid 36 through 88 )
15X-RAY DIFFRACTION15chain 'C' and (resid 89 through 141 )
16X-RAY DIFFRACTION16chain 'C' and (resid 142 through 155 )
17X-RAY DIFFRACTION17chain 'D' and (resid -1 through 20 )
18X-RAY DIFFRACTION18chain 'D' and (resid 21 through 69 )
19X-RAY DIFFRACTION19chain 'D' and (resid 70 through 141 )
20X-RAY DIFFRACTION20chain 'D' and (resid 142 through 155 )
21X-RAY DIFFRACTION21chain 'E' and (resid 2 through 27 )
22X-RAY DIFFRACTION22chain 'E' and (resid 28 through 73 )
23X-RAY DIFFRACTION23chain 'E' and (resid 74 through 93 )
24X-RAY DIFFRACTION24chain 'E' and (resid 94 through 111 )
25X-RAY DIFFRACTION25chain 'E' and (resid 112 through 132 )
26X-RAY DIFFRACTION26chain 'E' and (resid 133 through 196 )
27X-RAY DIFFRACTION27chain 'E' and (resid 197 through 224 )
28X-RAY DIFFRACTION28chain 'E' and (resid 225 through 281 )
29X-RAY DIFFRACTION29chain 'E' and (resid 282 through 335 )
30X-RAY DIFFRACTION30chain 'F' and (resid 0 through 27 )
31X-RAY DIFFRACTION31chain 'F' and (resid 28 through 87 )
32X-RAY DIFFRACTION32chain 'F' and (resid 88 through 122 )
33X-RAY DIFFRACTION33chain 'F' and (resid 123 through 254 )
34X-RAY DIFFRACTION34chain 'F' and (resid 255 through 305 )
35X-RAY DIFFRACTION35chain 'F' and (resid 306 through 335 )

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