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- PDB-6swr: Crystal structure of the lysosomal potassium channel MtTMEM175 T3... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6swr | |||||||||
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Title | Crystal structure of the lysosomal potassium channel MtTMEM175 T38A mutant soaked with zinc | |||||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() ![]() ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Brunner, J.D. / Jakob, R.P. / Schulze, T. / Neldner, Y. / Moroni, A. / Thiel, G. / Maier, T. / Schenck, S. | |||||||||
![]() | ![]() Title: Structural basis for ion selectivity in TMEM175 K + channels. Authors: Brunner, J.D. / Jakob, R.P. / Schulze, T. / Neldner, Y. / Moroni, A. / Thiel, G. / Maier, T. / Schenck, S. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.1 MB | Display | ![]() |
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PDB format | ![]() | 919.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6hd8C ![]() 6hd9C ![]() 6hdaC ![]() 6hdbC ![]() 6hdcC ![]() 1anfS ![]() 5jqhS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
-Antibody / Protein , 2 types, 4 molecules ADBE
#1: Antibody | ![]() Mass: 53267.004 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() Gene: malE, b4034, JW3994 / Plasmid: pBXNPHM3 / Strain: K12 / Production host: ![]() ![]() ![]() #2: Protein | Mass: 29450.943 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 23168 / DSM 4126 / NBRC 15989 / NCIMB 1408 / VKM B-1430 / H-43 Gene: Ftrac_2467 / Production host: ![]() ![]() ![]() |
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-Sugars , 2 types, 4 molecules ![](data/chem/img/LMT.gif)
#3: Polysaccharide | #4: Sugar | |
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-Non-polymers , 2 types, 32 molecules ![](data/chem/img/K.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/HOH.gif)
#5: Chemical | ChemComp-K / |
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#6: Water | ChemComp-HOH / ![]() |
-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.24 Å3/Da / Density % sol: 70.96 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 100 mM Tris-HCl pH 8.5, 150 mM NaCl, 150 mM MgCl2 and 28-30 % PEG400 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Feb 9, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 3.2→49.72 Å / Num. obs: 43526 / % possible obs: 99.8 % / Redundancy: 51.8 % / Biso Wilson estimate: 76.29 Å2 / CC1/2: 0.993 / Net I/σ(I): 6.5 |
Reflection shell | Resolution: 3.2→3.32 Å / Num. unique obs: 4510 / CC1/2: 0.663 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 1ANF, 5JQH Resolution: 3.2→49.72 Å / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 3.2→49.72 Å
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