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Yorodumi- PDB-6ss0: Structure of the arginase-2-inhibitory human antigen-binding frag... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6ss0 | ||||||
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Title | Structure of the arginase-2-inhibitory human antigen-binding fragment Fab C0021181 | ||||||
Components | (Fab C0021181 ...) x 2 | ||||||
Keywords | PROTEIN BINDING / arginase-2 inhibitor / IgG / antigen-binding fragment | ||||||
Function / homology | DI(HYDROXYETHYL)ETHER / TRIETHYLENE GLYCOL / SUCCINIC ACID Function and homology information | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.7 Å | ||||||
Authors | Burschowsky, D. / Addyman, A. / Fiedler, S. / Groves, M. / Haynes, S. / Seewooruthun, C. / Carr, M. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: Mabs Title: Structural and functional characterization of C0021158, a high-affinity monoclonal antibody that inhibits Arginase 2 function via a novel non-competitive mechanism of action. Authors: Austin, M. / Burschowsky, D. / Chan, D.T.Y. / Jenkinson, L. / Haynes, S. / Diamandakis, A. / Seewooruthun, C. / Addyman, A. / Fiedler, S. / Ryman, S. / Whitehouse, J. / Slater, L.H. / ...Authors: Austin, M. / Burschowsky, D. / Chan, D.T.Y. / Jenkinson, L. / Haynes, S. / Diamandakis, A. / Seewooruthun, C. / Addyman, A. / Fiedler, S. / Ryman, S. / Whitehouse, J. / Slater, L.H. / Hadjinicolaou, A.V. / Gileadi, U. / Gowans, E. / Shibata, Y. / Barnard, M. / Kaserer, T. / Sharma, P. / Luheshi, N.M. / Wilkinson, R.W. / Vaughan, T.J. / Holt, S.V. / Cerundolo, V. / Carr, M.D. / Groves, M.A.T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6ss0.cif.gz | 338.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6ss0.ent.gz | Display | PDB format | |
PDBx/mmJSON format | 6ss0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ss/6ss0 ftp://data.pdbj.org/pub/pdb/validation_reports/ss/6ss0 | HTTPS FTP |
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-Related structure data
Related structure data | 6srvSC 6srxC 6ss2C 6ss4C 6tulC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
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-Components
-Antibody , 2 types, 4 molecules HHHIIILLLMMM
#1: Antibody | Mass: 24819.836 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: Amino acids are numbered according to the Kabat numbering scheme. Source: (gene. exp.) Homo sapiens (human) / Plasmid: pEU1.3 fab / Cell line (production host): CHO / Production host: Cricetulus griseus (Chinese hamster) / Strain (production host): ExpiCHO #2: Antibody | Mass: 23124.482 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: Amino acids are numbered according to the Kabat numbering scheme. Residue 10 would not be present in the Kabat scheme, but due to problems with non-continuous numbering in L-peptides, we ...Details: Amino acids are numbered according to the Kabat numbering scheme. Residue 10 would not be present in the Kabat scheme, but due to problems with non-continuous numbering in L-peptides, we included it. Therefore, residues 2-10 should be read as 1-9, per Kabat. Source: (gene. exp.) Homo sapiens (human) / Plasmid: pEU1.3 fab / Cell line (production host): CHO / Production host: Cricetulus griseus (Chinese hamster) / Strain (production host): ExpiCHO |
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-Non-polymers , 5 types, 586 molecules
#3: Chemical | ChemComp-CL / #4: Chemical | #5: Chemical | #6: Chemical | ChemComp-PGE / | #7: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.13 Å3/Da / Density % sol: 42.33 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 100 mM SPG pH 7.0 25% PEG1500 PACT A4 SPG: succinic acid, phosphate, glycine |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-1 / Wavelength: 0.97242 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Nov 11, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97242 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→46.548 Å / Num. obs: 88773 / % possible obs: 100 % / Redundancy: 6.4 % / CC1/2: 1 / Rmerge(I) obs: 0.15 / Net I/σ(I): 5.9 |
Reflection shell | Resolution: 1.7→1.73 Å / Redundancy: 5.4 % / Rmerge(I) obs: 2.59 / Mean I/σ(I) obs: 0.9 / Num. unique obs: 4541 / CC1/2: 0.36 / % possible all: 99.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6SRV Resolution: 1.7→46.544 Å / Cor.coef. Fo:Fc: 0.966 / Cor.coef. Fo:Fc free: 0.951 / SU B: 3.339 / SU ML: 0.101 / Cross valid method: FREE R-VALUE / ESU R: 0.117 / ESU R Free: 0.113 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 26.241 Å2
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Refinement step | Cycle: LAST / Resolution: 1.7→46.544 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 20
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