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Open data
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Basic information
Entry | Database: PDB / ID: 6sqp | |||||||||
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Title | Crystal structure of Cat MDM2-S429E RING domain homodimer | |||||||||
![]() | (E3 ubiquitin-protein ligase ...) x 3 | |||||||||
![]() | ![]() ![]() ![]() ![]() ![]() | |||||||||
Function / homology | ![]() regulation of biological quality / ribonucleoprotein complex binding / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Magnussen, H.M. / Ahmed, S.F. / Huang, D.T. | |||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: Structural basis for DNA damage-induced phosphoregulation of MDM2 RING domain. Authors: Magnussen, H.M. / Ahmed, S.F. / Sibbet, G.J. / Hristova, V.A. / Nomura, K. / Hock, A.K. / Archibald, L.J. / Jamieson, A.G. / Fushman, D. / Vousden, K.H. / Weissman, A.M. / Huang, D.T. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 127.2 KB | Display | ![]() |
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PDB format | ![]() | 97.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6sqoC ![]() 6sqrC ![]() 6sqsC ![]() 5mnjS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
-E3 ubiquitin-protein ligase ... , 3 types, 4 molecules ABDC
#1: Protein | Mass: 7152.696 Da / Num. of mol.: 1 / Mutation: S429E, G443T Source method: isolated from a genetically manipulated source Details: Residues 422-491 and contains S429E and G443T mutation. GS at the N-terminus resulted from cloning. Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() References: UniProt: Q7YRZ8, RING-type E3 ubiquitin transferase | ||
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#2: Protein | Mass: 7720.328 Da / Num. of mol.: 2 / Mutation: S429E, G443T Source method: isolated from a genetically manipulated source Details: Residues 422-491 and contains S429E and G443T. GS at the N-terminus resulted from cloning. Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() References: UniProt: Q7YRZ8, RING-type E3 ubiquitin transferase #3: Protein | | Mass: 7378.926 Da / Num. of mol.: 1 / Mutation: S429E, G443T Source method: isolated from a genetically manipulated source Details: Residues 422-491 and contains S429E and G443T. GS at the N-terminus resulted from cloning. Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() References: UniProt: Q7YRZ8, RING-type E3 ubiquitin transferase |
-Non-polymers , 4 types, 254 molecules ![](data/chem/img/ZN.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/NO3.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/NO3.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-ZN / #5: Chemical | ![]() #6: Chemical | ChemComp-NO3 / | ![]() #7: Water | ChemComp-HOH / | ![]() |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 39.13 % |
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Crystal grow![]() | Temperature: 292 K / Method: vapor diffusion / Details: 0.1 M MMT, pH 9.0 and 25% (w/v) PEG1500 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Jul 7, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.21→23.53 Å / Num. obs: 71796 / % possible obs: 98.3 % / Redundancy: 3.2 % / CC1/2: 0.991 / Net I/σ(I): 5.1 |
Reflection shell | Resolution: 1.21→1.24 Å / Num. unique obs: 5274 / CC1/2: 0.608 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 5MNJ Resolution: 1.21→23.53 Å / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 1.21→23.53 Å
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