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Yorodumi- PDB-6q2i: Solution state NMR structures of the RNA recognition motif (RRM) ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6q2i | ||||||
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Title | Solution state NMR structures of the RNA recognition motif (RRM) domain of human CstF-64 | ||||||
Components | Cleavage stimulation factor subunit 2 | ||||||
Keywords | TRANSCRIPTION / RNA recognition motif / RNA cleavage and polyadenylation / RNA BINDING PROTEIN | ||||||
Function / homology | Function and homology information cleavage body / Processing of Intronless Pre-mRNAs / mRNA cleavage and polyadenylation specificity factor complex / mRNA 3'-end processing / mRNA 3'-end processing / tRNA processing in the nucleus / RNA Polymerase II Transcription Termination / Processing of Capped Intron-Containing Pre-mRNA / cellular response to nerve growth factor stimulus / nuclear body ...cleavage body / Processing of Intronless Pre-mRNAs / mRNA cleavage and polyadenylation specificity factor complex / mRNA 3'-end processing / mRNA 3'-end processing / tRNA processing in the nucleus / RNA Polymerase II Transcription Termination / Processing of Capped Intron-Containing Pre-mRNA / cellular response to nerve growth factor stimulus / nuclear body / mRNA binding / RNA binding / nucleoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Latham, M.P. / Masoumzadeh, E. | ||||||
Funding support | United States, 1items
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Citation | Journal: Nucleic Acids Res. / Year: 2020 Title: A missense mutation in the CSTF2 gene that impairs the function of the RNA recognition motif and causes defects in 3' end processing is associated with intellectual disability in humans. Authors: Grozdanov, P.N. / Masoumzadeh, E. / Kalscheuer, V.M. / Bienvenu, T. / Billuart, P. / Delrue, M.A. / Latham, M.P. / MacDonald, C.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6q2i.cif.gz | 327.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6q2i.ent.gz | 271.7 KB | Display | PDB format |
PDBx/mmJSON format | 6q2i.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/q2/6q2i ftp://data.pdbj.org/pub/pdb/validation_reports/q2/6q2i | HTTPS FTP |
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-Related structure data
Related structure data | 6tzeC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 12095.447 Da / Num. of mol.: 1 / Fragment: RNA recognition motif, residues 1-108 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CSTF2 / Plasmid: pET22 / Production host: Escherichia coli (E. coli) / References: UniProt: P33240 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution Contents: 980 uM [U-100% 13C; U-100% 15N] RNA Recognition Motif, 90% H2O/10% D2O Label: 15N, 13C_sample / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 980 uM / Component: RNA Recognition Motif / Isotopic labeling: [U-100% 13C; U-100% 15N] |
Sample conditions | Ionic strength: 10 mM / Label: conditions_1 / pH: 6 / Pressure: 1 atm / Temperature: 303 K |
-NMR measurement
NMR spectrometer | Type: Agilent DD2 / Manufacturer: Agilent / Model: DD2 / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 5 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 10000 / Conformers submitted total number: 10 |