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Yorodumi- PDB-6pd6: Crystal Structure of a H5N1 influenza virus hemagglutinin at pH 7.0 -
+Open data
-Basic information
Entry | Database: PDB / ID: 6pd6 | ||||||
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Title | Crystal Structure of a H5N1 influenza virus hemagglutinin at pH 7.0 | ||||||
Components | Hemagglutinin | ||||||
Keywords | VIRAL PROTEIN / Influenza / H5 | ||||||
Function / homology | Function and homology information clathrin-dependent endocytosis of virus by host cell / host cell surface receptor binding / apical plasma membrane / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / virion attachment to host cell / host cell plasma membrane Similarity search - Function | ||||||
Biological species | Influenza A virus | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.87 Å | ||||||
Authors | Antanasijevic, A. / Durst, M.A. / Lavie, A. / Caffrey, M. | ||||||
Citation | Journal: J.Struct.Biol. / Year: 2020 Title: Identification of a pH sensor in Influenza hemagglutinin using X-ray crystallography. Authors: Antanasijevic, A. / Durst, M.A. / Lavie, A. / Caffrey, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6pd6.cif.gz | 120.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6pd6.ent.gz | 90.7 KB | Display | PDB format |
PDBx/mmJSON format | 6pd6.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pd/6pd6 ftp://data.pdbj.org/pub/pdb/validation_reports/pd/6pd6 | HTTPS FTP |
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-Related structure data
Related structure data | 6pcxC 6pd3C 6pd5C 2fk0S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 59893.277 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Influenza A virus (A/chicken/Vietnam/4/2003(H5N1)) Strain: A/chicken/Vietnam/4/2003(H5N1) / Gene: HA / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: Q1KHJ8 | ||||||||
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#2: Chemical | ChemComp-SO4 / #3: Chemical | #4: Sugar | ChemComp-NAG / #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.07 Å3/Da / Density % sol: 69.77 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 100 mM cacodylate, pH 7.0, 200 mM sodium chloride, 2 M ammonium sulfate, 10% glycerol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 21-ID-G / Wavelength: 0.979 Å |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Mar 3, 2016 |
Radiation | Monochromator: diamond(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.79→30 Å / Num. obs: 24399 / % possible obs: 98.2 % / Redundancy: 6.6 % / CC1/2: 0.997 / Rrim(I) all: 0.166 / Net I/σ(I): 14.79 |
Reflection shell | Resolution: 2.79→2.95 Å / Redundancy: 6.18 % / Mean I/σ(I) obs: 1.54 / Num. unique obs: 3544 / CC1/2: 0.542 / Rrim(I) all: 1.397 / % possible all: 90.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 2FK0 Resolution: 2.87→29.58 Å / Cor.coef. Fo:Fc: 0.948 / Cor.coef. Fo:Fc free: 0.895 / SU B: 23 / SU ML: 0.364 / SU R Cruickshank DPI: 0.6395 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.64 / ESU R Free: 0.382 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 230.15 Å2 / Biso mean: 79.781 Å2 / Biso min: 43.01 Å2
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Refinement step | Cycle: final / Resolution: 2.87→29.58 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.874→2.948 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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