+Open data
-Basic information
Entry | Database: PDB / ID: 6okn | ||||||
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Title | OX40R (TNFRSF4) bound to Fab 1A7 | ||||||
Components |
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Keywords | IMMUNE SYSTEM / TNFRSF4 / CD134 / OX40R / Fab | ||||||
Function / homology | Function and homology information tumor necrosis factor receptor activity / TNFs bind their physiological receptors / positive regulation of immunoglobulin production / T cell proliferation / positive regulation of B cell proliferation / negative regulation of DNA-binding transcription factor activity / virus receptor activity / inflammatory response / immune response / external side of plasma membrane ...tumor necrosis factor receptor activity / TNFs bind their physiological receptors / positive regulation of immunoglobulin production / T cell proliferation / positive regulation of B cell proliferation / negative regulation of DNA-binding transcription factor activity / virus receptor activity / inflammatory response / immune response / external side of plasma membrane / negative regulation of DNA-templated transcription / cell surface / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 3.25 Å | ||||||
Authors | Ultsch, M.H. / Boenig, G. / Harris, S.F. | ||||||
Citation | Journal: Mabs / Year: 2019 Title: Tetravalent biepitopic targeting enables intrinsic antibody agonism of tumor necrosis factor receptor superfamily members. Authors: Yang, Y. / Yeh, S.H. / Madireddi, S. / Matochko, W.L. / Gu, C. / Pacheco Sanchez, P. / Ultsch, M. / De Leon Boenig, G. / Harris, S.F. / Leonard, B. / Scales, S.J. / Zhu, J.W. / Christensen, ...Authors: Yang, Y. / Yeh, S.H. / Madireddi, S. / Matochko, W.L. / Gu, C. / Pacheco Sanchez, P. / Ultsch, M. / De Leon Boenig, G. / Harris, S.F. / Leonard, B. / Scales, S.J. / Zhu, J.W. / Christensen, E. / Hang, J.Q. / Brezski, R.J. / Marsters, S. / Ashkenazi, A. / Sukumaran, S. / Chiu, H. / Cubas, R. / Kim, J.M. / Lazar, G.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6okn.cif.gz | 415.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6okn.ent.gz | 356.8 KB | Display | PDB format |
PDBx/mmJSON format | 6okn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ok/6okn ftp://data.pdbj.org/pub/pdb/validation_reports/ok/6okn | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Antibody | Mass: 24206.057 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) #2: Antibody | Mass: 23531.135 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli) #3: Protein | Mass: 17697.852 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: TNFRSF4, TXGP1L / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P43489 #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.8 Å3/Da / Density % sol: 56.03 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: tbd |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å | ||||||||||||||||||||||||
Detector | Type: RAYONIX MX300HE / Detector: CCD / Date: Jun 22, 2016 | ||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | ||||||||||||||||||||||||
Reflection | Resolution: 3.25→29.49 Å / Num. obs: 24096 / % possible obs: 99.8 % / Redundancy: 13.3 % / Biso Wilson estimate: 85.31 Å2 / CC1/2: 0.997 / Rmerge(I) obs: 0.166 / Rpim(I) all: 0.047 / Rrim(I) all: 0.172 / Net I/σ(I): 9.2 | ||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Phasing
Phasing | Method: molecular replacement |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.25→29.49 Å / Cor.coef. Fo:Fc: 0.931 / Cor.coef. Fo:Fc free: 0.923 / Rfactor Rfree error: 0 / Cross valid method: THROUGHOUT / σ(F): 0 / SU Rfree Blow DPI: 0.42
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Displacement parameters | Biso max: 256.96 Å2 / Biso mean: 120.06 Å2 / Biso min: 50.28 Å2
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Refine analyze | Luzzati coordinate error obs: 0.41 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 3.25→29.49 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.25→3.39 Å / Rfactor Rfree error: 0 / Total num. of bins used: 12
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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