PDB-6mwn: Crystal structure of hepatitis A virus IRES domain V in complex w...

ID or keywords:

Entry

Database: PDB / ID: 6mwn

Title

Crystal structure of hepatitis A virus IRES domain V in complex with Fab HAVx

Components

Fab HAVx Heavy Chain
Fab HAVx Light Chain
HAV dV RNA (92-MER)

Keywords

RNA/Immune System /

RNA / Chaperone Assisted RNA Crystallography / Phage Display /

Hepatitis A Virus / IRES / Fab Antibody / RNA-Immune System Complex

Function / homology

Immunoglobulins /

Immunoglobulin-like /

Sandwich / Mainly Beta / : /

RNA / RNA (> 10)

Function and homology information

Biological species

Homo sapiens (human)

Hepatovirus A

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.838 Å

Authors

Koirala, D. / Shao, Y. / Piccirilli, J.A.

Funding support

United States, 2items

Organization	Grant number	Country
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)	R01AI081987	United States
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)	R01GM102489	United States

Citation

Journal: Nat Commun / Year: 2019
Title: A conserved RNA structural motif for organizing topology within picornaviral internal ribosome entry sites.
Authors: Koirala, D. / Shao, Y. / Koldobskaya, Y. / Fuller, J.R. / Watkins, A.M. / Shelke, S.A. / Pilipenko, E.V. / Das, R. / Rice, P.A. / Piccirilli, J.A.

History

Deposition	Oct 29, 2018	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Aug 14, 2019	Provider: repository / Type: Initial release
Revision 1.1	Aug 21, 2019	Group: Data collection / Database references / Category: citation / citation_author Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID
Revision 1.2	Dec 18, 2019	Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.3	Oct 11, 2023	Group: Data collection / Database references / Refinement description Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	6mwn.cif.gz	565.1 KB	Display	PDBx/mmCIF format
PDB format	pdb6mwn.ent.gz	459.8 KB	Display	PDB format
PDBx/mmJSON format	6mwn.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/mw/6mwn ftp://data.pdbj.org/pub/pdb/validation_reports/mw/6mwn	HTTPS FTP

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Related structure data

Related structure data	3ivkS 4kzeS S: Starting model for refinement
Similar structure data	5ot0-d 4zp4-d 5glz-d 4zqd-d 7kqy-d 1pfz-d 6zjx-1 5fjy-d Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#121 - Jan 2010 70S Ribosomes similarity (1) #130 - Oct 2010 Riboswitches similarity (1) #16 - Apr 2001 Aminoacyl-tRNA Synthetases similarity (1) #157 - Jan 2013 Transfer-Messenger RNA similarity (1) #229 - Jan 2019 Fluorescent RNA Aptamers similarity (1) #181 - Jan 2015 Cascade and CRISPR similarity (1) #260 - Aug 2021 Ribonuclease P similarity (1) #253 - Jan 2021 Expressome similarity (1) #146 - Feb 2012 Aminoglycoside Antibiotics similarity (1) #15 - Mar 2001 Transfer RNA similarity (1) #249 - Sep 2020 SARS-CoV-2 RNA-dependent RNA Polymerase similarity (1) #87 - Mar 2007 Zinc Fingers similarity (1) #10 - Oct 2000 Ribosome similarity (1) #210 - Jun 2017 Adenine Riboswitch in Action similarity (1) #262 - Oct 2021 Fifty Years of Open Access to PDB Structures similarity (1) #98 - Feb 2008 Small Interfering RNA similarity (1) #105 - Sep 2008 Ribonuclease A similarity (1) #245 - May 2020 Spliceosomes similarity (1) #65 - May 2005 Self-splicing RNA similarity (1) #226 - Oct 2018 Aminoglycoside Antibiotics and Resistance similarity (1) #21 - Sep 2001 Antibodies similarity (1) #89 - May 2007 Aconitase and Iron Regulatory Protein 1 similarity (1) #81 - Sep 2006 Elongation Factors similarity (1) #106 - Oct 2008 Poly(A) Polymerase similarity (1) #266 - Feb 2022 Oligosaccharyltransferase similarity (1) #143 - Nov 2011 Toll-like Receptors similarity (1) #161 - May 2013 Ricin similarity (1) #230 - Feb 2019 Initiation Factor eIF4E similarity (1) #104 - Aug 2008 Selenocysteine Synthase similarity (1) #227 - Nov 2018 Telomerase similarity (1) #256 - Apr 2021 SARS-CoV-2 Spike and Antibodies similarity (1) #241 - Jan 2020 Twenty Years of Molecules similarity (1)

PDB pages

PDBj /

wwPDB /

NCBI

Related items in Molecule of the Month

#121 - Jan 2010
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Riboswitches
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Transfer-Messenger RNA
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Deposited unit

A: HAV dV RNA (92-MER)

B: HAV dV RNA (92-MER)

C: Fab HAVx Heavy Chain

D: Fab HAVx Light Chain

H: Fab HAVx Heavy Chain

L: Fab HAVx Light Chain

167 kDa, 6 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software
Evidence: native gel electrophoresis, gel filtration, SAXS

Unit cell

Length a, b, c (Å)	74.149, 100.582, 236.594
Angle α, β, γ (deg.)	90.00, 90.00, 90.00
Int Tables number	18
Space group name H-M	P2₁22₁

#1: RNA chain	HAV dV RNA (92-MER) Mass: 29750.332 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: Hepatitis A Virus IRES Domain V / Source: (synth.) Hepatovirus A / References: GenBank: 329582
#2: Antibody	Fab HAVx Heavy Chain Mass: 27588.012 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: Antibody fragment (Fab) that binds to domain V of Hepatitis A Virus IRES Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli)
#3: Antibody	Fab HAVx Light Chain Mass: 26351.588 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Production host: Escherichia coli (E. coli)
#4: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 180 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.63 Å³/Da / Density % sol: 53.27 %
Crystal grow	Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 0.2 M ammonium sulfate, 0.1 M HEPES, pH 7.5, 25% PEG 3350

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Data collection

Diffraction	Mean temperature: 80 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: APS / Beamline: 23-ID-B / Wavelength: 1.033202 Å
Detector	Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 10, 2017
Radiation	Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1.033202 Å / Relative weight: 1
Reflection	Resolution: 2.838→29.607 Å / Num. obs: 42565 / % possible obs: 99.6 % / Redundancy: 6.8 % / B_iso Wilson estimate: 74.9 Å² / CC_1/2: 0.999 / R_merge(I) obs: 0.1004 / R_pim(I) all: 0.04158 / R_rim(I) all: 0.1088 / Net I/av σ(I): 13.89 / Net I/σ(I): 13.89
Reflection shell	Resolution: 2.838→2.94 Å / Redundancy: 6.8 % / Mean I/σ(I) obs: 1.38 / Num. unique obs: 4057 / CC_1/2: 0.595 / R_pim(I) all: 0.524 / % possible all: 96.4

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Processing

Software

Name	Version	Classification
PHENIX	(1.14)	refinement
HKL-2000		data reduction
Aimless		data scaling
PHENIX		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: Fab BL3-6 4KZE, 3IVK

4kze

3ivk

Resolution: 2.838→29.607 Å / SU ML: 0.39 / Cross valid method: FREE R-VALUE / σ(F): 1.38 / Phase error: 27.26

	R_factor	Num. reflection	% reflection
R_free	0.2522	1718	4.04 %
R_work	0.1856	-	-
obs	0.1856	42533	99.54 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å

Refinement step

Cycle: LAST / Resolution: 2.838→29.607 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	6696	3934	0	180	10810

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.009	11256
X-RAY DIFFRACTION	f_angle_d	1.18	16184
X-RAY DIFFRACTION	f_dihedral_angle_d	14.003	6308
X-RAY DIFFRACTION	f_chiral_restr	0.055	1960
X-RAY DIFFRACTION	f_plane_restr	0.007	1366

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.8381-2.9215	0.3721	135	0.3021	3208	X-RAY DIFFRACTION	96
2.9215-3.0157	0.3313	141	0.2816	3347	X-RAY DIFFRACTION	100
3.0157-3.1234	0.3667	143	0.2591	3387	X-RAY DIFFRACTION	100
3.1234-3.2483	0.2922	141	0.2187	3370	X-RAY DIFFRACTION	100
3.2483-3.3959	0.3219	143	0.2033	3380	X-RAY DIFFRACTION	100
3.3959-3.5747	0.2422	142	0.1876	3395	X-RAY DIFFRACTION	100
3.5747-3.7982	0.2383	141	0.1746	3351	X-RAY DIFFRACTION	100
3.7982-4.0908	0.2156	144	0.1629	3406	X-RAY DIFFRACTION	100
4.0908-4.5012	0.2226	143	0.1547	3415	X-RAY DIFFRACTION	100
4.5012-5.1496	0.2248	145	0.1484	3444	X-RAY DIFFRACTION	100
5.1496-6.4767	0.2455	146	0.177	3482	X-RAY DIFFRACTION	100
6.4767-29.609	0.2574	154	0.1775	3630	X-RAY DIFFRACTION	100

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.2866	-0.2283	-0.3197	0.6401	-1.1458	1.9446	-0.2525	-0.5734	0.4343	0.3456	0.5698	-0.0804	-0.6194	0.2693	-0.3834	1.3964	-0.1424	-0.103	1.2299	-0.229	0.6381	17.8697	21.1568	55.3944
2	2.5509	0.0165	1.2394	0.6855	1.4363	5.4327	0.1015	-0.8737	0.1661	0.2409	0.1716	-0.3351	-0.7043	-0.5719	-0.7908	1.9839	-0.0758	0.1781	1.2617	0.0176	1.0381	13.3806	39.0235	51.5643
3	1.0943	-0.8339	-0.8224	1.047	1.2939	2.3214	0.0154	-0.7638	0.4459	0.5555	0.4105	-0.3759	-0.9691	0.1163	-0.4272	1.6103	0.0392	-0.1067	1.4191	-0.2046	0.7509	18.3886	25.5031	61.1247
4	1.8115	1.2395	0.5319	3.2232	0.2291	-0.1129	-0.1294	0.4986	0.0334	-0.2784	0.1409	-0.4585	0.0498	-0.1057	-0.0694	0.714	0.0069	0.086	0.7179	-0.018	0.4854	17.6652	30.0226	-6.4655
5	0.474	1.5215	-0.5137	6.1074	-1.7963	0.8598	0.6096	0.5308	0.2598	0.8572	-0.6627	-1.8675	0.4619	0.4815	-0.0739	1.0394	-0.1545	-0.1493	0.8781	0.1429	1.1215	17.8579	22.8026	17.6631
6	1.0882	0.0521	0.4282	1.2734	0.8451	-0.216	-0.0145	0.5073	0.079	-0.2221	0.105	-0.3936	-0.0537	0.0553	-0.2129	0.8254	-0.0268	0.0689	0.7862	0.0594	0.4839	15.8016	17.912	-12.616
7	0.3135	0.5552	0.4082	1.8887	2.1564	4.5099	0.2224	-0.9635	0.6536	1.3342	0.1634	-1.4035	0.3253	0.3638	-0.4272	0.6817	-0.1565	-0.2857	0.704	-0.1819	1.2422	34.479	49.6843	20.1357
8	1.9306	0.5231	0.374	4.428	2.4254	4.814	0.6699	-0.1319	0.2532	0.0043	-0.3916	-1.664	0.1007	0.6631	-0.566	0.5669	-0.0551	-0.0844	0.5668	-0.0179	1.1455	35.3103	50.7228	11.8902
9	1.7498	0.795	0.5564	3.5206	2.4372	4.7689	0.1175	-0.3844	0.2408	0.4076	0.183	-0.8796	0.0011	0.0732	-0.227	0.4868	-0.0772	-0.1143	0.5186	0.0173	0.9968	28.1466	49.0537	15.1187
10	0.6585	0.1545	0.0851	0.1292	0.4427	1.5239	0.565	0.0694	0.1842	0.601	-0.1058	-0.6216	-0.176	0.7114	-0.0373	1.5878	-0.5157	-0.8133	0.3933	-0.5058	2.3052	34.887	69.7699	38.7834
11	1.9602	0.5185	0.2634	6.0169	1.3662	5.144	1.326	-1.0319	0.3737	1.6743	0.3843	-0.3725	0.3798	-0.8904	-0.1603	2.088	-0.6099	-0.3645	1.4828	0.2938	1.1055	19.5735	67.2854	51.076
12	1.2298	0.76	-0.0363	3.0805	1.0408	1.6161	0.2387	0.2159	-0.7103	0.4893	0.3543	-1.0867	0.1571	0.4112	0.0787	2.3228	-0.6263	-0.7751	0.2713	-0.7141	1.3208	29.679	59.9641	39.4626
13	3.1169	-1.8014	0.2743	3.2916	3.4606	6.1687	-0.1946	0.1433	-0.8321	1.9106	-0.4888	-0.85	-0.0129	0.4495	-0.0806	1.4589	-0.0698	-0.3002	0.747	-0.0264	1.1623	23.1638	65.4044	38.9025
14	0.5847	0.2059	0.8875	2.9351	2.0011	3.2457	0.6216	-0.6715	-0.7157	2.3624	-0.4381	-0.8184	0.8742	0.3707	0.4814	2.778	-0.5909	-0.1432	0.8367	0.0973	1.0375	21.1781	60.9194	51.2631
15	0.58	-0.8154	0.3938	5.5013	3.32	3.957	0.8812	-0.4043	-0.278	1.0075	0.7558	-1.4995	1.1771	1.0536	-0.4579	1.7443	-0.4506	-0.4892	1.3754	-0.057	1.4353	32.6442	61.6518	46.6312
16	1.4237	-1.2267	-0.2926	4.8692	2.6027	5.3861	0.4108	0.1151	0.4826	0.2085	-0.3389	0.3258	-0.5394	-0.7275	0.0935	0.4915	-0.071	0.0364	0.6068	0.037	0.6837	9.2979	52.9072	12.6694
17	1.5492	-0.6229	-0.4241	4.8818	1.7548	4.8323	0.3417	-0.159	0.6156	0.6688	-0.4201	0.5027	1.2647	0.0252	-0.0386	0.6111	-0.1346	-0.0245	0.5608	0.0841	0.6794	10.6015	45.3442	17.2262
18	1.3117	-0.6348	-0.8416	4.7138	2.5694	5.575	0.094	0.1022	1.1158	0.7216	0.5761	-0.4942	0.6549	-0.3348	-0.1036	0.6143	-0.0688	-0.0587	0.4942	0.028	0.7531	14.1334	51.4532	15.755
19	3.1303	-2.5325	1.1623	2.8139	1.0157	5.5135	-0.0037	-0.8111	0.3925	1.6108	0.5908	0.2069	-0.2822	-1.0576	0.8223	1.338	-0.3334	0.2699	0.7463	-0.3738	0.8083	3.9149	60.6777	32.3545
20	1.9215	0.3041	-1.0132	2.1769	2.559	4.2522	1.0937	-0.3822	0.568	2.0787	-0.2739	-0.3628	0.2811	-0.2178	0.0235	2.0093	-0.2464	-0.1473	0.66	-0.1258	1.079	16.7889	73.4617	45.2306
21	2.237	0.3486	-0.0557	5.8893	3.6435	5.7227	0.266	0.1858	0.2024	1.6146	0.3485	-0.5954	-1.399	-0.3838	-0.1165	1.8013	0.2083	0.2185	0.9615	0.1116	0.8921	13.3551	71.0736	38.4381
22	0.7405	0.0612	0.515	3.7236	2.4342	3.5088	0.0419	-0.4042	0.8896	1.2928	-0.2209	-0.103	0.0326	-0.1517	-0.0592	1.995	-0.2176	0.0058	0.9169	-0.2057	1.0241	14.6206	78.6079	47.8373
23	2.739	0.2734	-0.3356	4.1589	1.9236	6.4298	0.2332	-0.1588	-0.0868	0.2799	0.0884	-0.8332	-0.2135	0.7155	-0.2775	0.5252	-0.1105	-0.1249	0.7853	-0.1055	0.6096	32.9389	1.9067	32.9444
24	1.3767	0.7973	-0.2177	1.8913	3.161	7.8868	-0.0018	-0.5644	-0.4911	0.3618	0.2178	-0.026	0.6663	0.8011	-0.495	0.5844	0.184	0.0541	0.6723	0.0598	0.9771	34.3249	-18.2135	9.0913
25	4.9515	-2.0767	0.0115	5.9626	0.1996	6.6302	0.5908	0.6418	0.2993	-0.5959	-0.4519	0.0728	0.4996	-0.4368	-0.2516	0.5081	0.0657	0.0665	0.5514	0.0825	0.564	18.9751	-15.7645	-2.7834
26	3.6911	0.8308	1.2512	2.6098	0.5493	5.3288	-0.3115	0.01	0.4861	-0.1081	0.2585	-0.1262	-0.2525	0.3461	-0.0637	0.4891	0.0674	0.0875	0.4443	0.0385	0.679	26.168	-10.7382	2.9906
27	2.9121	-0.1934	2.7092	3.3071	1.1344	6.8395	0.6811	-0.5302	-0.13	0.6579	-0.4045	0.042	0.8666	0.0837	0.1491	0.6614	-0.124	0.0926	0.6167	0.1722	0.3389	7.0322	-3.6893	33.9525
28	2.3563	0.4937	1.553	2.9274	1.5088	3.3634	0.0249	-0.354	-0.0608	0.3106	0.0012	-0.1219	-0.5248	0.0574	0.0079	0.5659	-0.0014	0.0166	0.5859	-0.0188	0.2882	11.7557	4.4893	32.1127
29	3.007	-2.88	-0.6691	3.0352	1.2649	8.3813	-0.0259	-0.9821	-0.4352	1.4899	1.1644	-1.41	-0.3821	-0.236	-0.1364	0.7914	-0.0192	-0.1135	0.9187	0.1103	0.5767	20.0705	0.3397	42.0598
30	0.6264	1.4032	1.0393	4.0324	4.2511	6.7868	0.0642	-0.0275	-0.5483	-0.2455	0.2842	-0.2451	0.0237	-0.4272	0.1051	0.5088	0.105	0.0055	0.4484	-0.0663	0.4517	4.0501	-8.483	16.4931
31	2.8114	-1.8403	-0.1739	5.2372	1.9919	3.186	-0.0613	-0.094	-0.0028	-0.128	0.3195	-0.6246	0.2813	0.1742	-0.129	0.5031	0.0013	0.0734	0.3248	0.0049	0.4719	16.4532	-21.9137	3.7842
32	4.5434	-0.6365	-0.463	4.8269	1.5308	2.5781	-0.0339	0.0321	-0.431	-0.5909	0.0754	-0.0669	0.147	0.0007	-0.046	0.569	-0.0534	0.1119	0.4388	0.0322	0.4306	13.8643	-24.4483	4.4602

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 594 through 623 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 624 through 653 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 654 through 684 )
4	X-RAY DIFFRACTION	4	chain 'B' and (resid 594 through 623 )
5	X-RAY DIFFRACTION	5	chain 'B' and (resid 624 through 633 )
6	X-RAY DIFFRACTION	6	chain 'B' and (resid 634 through 684 )
7	X-RAY DIFFRACTION	7	chain 'C' and (resid 4 through 43 )
8	X-RAY DIFFRACTION	8	chain 'C' and (resid 44 through 86 )
9	X-RAY DIFFRACTION	9	chain 'C' and (resid 87 through 125 )
10	X-RAY DIFFRACTION	10	chain 'C' and (resid 126 through 138 )
11	X-RAY DIFFRACTION	11	chain 'C' and (resid 139 through 159 )
12	X-RAY DIFFRACTION	12	chain 'C' and (resid 160 through 171 )
13	X-RAY DIFFRACTION	13	chain 'C' and (resid 172 through 189 )
14	X-RAY DIFFRACTION	14	chain 'C' and (resid 190 through 208 )
15	X-RAY DIFFRACTION	15	chain 'C' and (resid 209 through 228 )
16	X-RAY DIFFRACTION	16	chain 'D' and (resid 2 through 39 )
17	X-RAY DIFFRACTION	17	chain 'D' and (resid 40 through 76 )
18	X-RAY DIFFRACTION	18	chain 'D' and (resid 77 through 102 )
19	X-RAY DIFFRACTION	19	chain 'D' and (resid 103 through 114 )
20	X-RAY DIFFRACTION	20	chain 'D' and (resid 115 through 151 )
21	X-RAY DIFFRACTION	21	chain 'D' and (resid 152 through 173 )
22	X-RAY DIFFRACTION	22	chain 'D' and (resid 174 through 214 )
23	X-RAY DIFFRACTION	23	chain 'H' and (resid 4 through 125 )
24	X-RAY DIFFRACTION	24	chain 'H' and (resid 126 through 138 )
25	X-RAY DIFFRACTION	25	chain 'H' and (resid 139 through 159 )
26	X-RAY DIFFRACTION	26	chain 'H' and (resid 160 through 228 )
27	X-RAY DIFFRACTION	27	chain 'L' and (resid 2 through 26 )
28	X-RAY DIFFRACTION	28	chain 'L' and (resid 27 through 91 )
29	X-RAY DIFFRACTION	29	chain 'L' and (resid 92 through 102 )
30	X-RAY DIFFRACTION	30	chain 'L' and (resid 103 through 114 )
31	X-RAY DIFFRACTION	31	chain 'L' and (resid 115 through 151 )
32	X-RAY DIFFRACTION	32	chain 'L' and (resid 152 through 214 )

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