Details | Type | Solution-ID | Contents | Label | Solvent system |
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micelle1 | 20 mM HEPES, 100 uM [2,3,3-2H,15N]-Leu beta 2 adrenergic receptor (A59C-MTSL), 95% H2O/5% D2O | A59C-MTSL (paramagnetic) | 95% H2O/5% D2Omicelle2 | 20 mM HEPES, 100 uM [2,3,3-2H,15N]-Leu beta 2 adrenergic receptor (A59C-MTSL), 1 mM ascorbate, 95% H2O/5% D2O | A59C-MTSL (diamagnetic) | 95% H2O/5% D2Omicelle3 | 20 mM HEPES, 100 uM [2,3,3-2H,15N]-Leu beta 2 adrenergic receptor (T136C-MTSL), 95% H2O/5% D2O | T136C-MTSL (paramagnetic) | 95% H2O/5% D2Omicelle4 | 20 mM HEPES, 100 uM [2,3,3-2H,15N]-Leu beta 2 adrenergic receptor (T136C-MTSL), 1 mM ascorbate, 95% H2O/5% D2O | T136C-MTSL (diamagnetic) | 95% H2O/5% D2Omicelle5 | 20 mM HEPES, 100 uM [2,3,3-2H,15N]-Leu beta 2 adrenergic receptor (N148C-MTSL), 95% H2O/5% D2O | N148C-MTSL (paramagnetic) | 95% H2O/5% D2Omicelle6 | 20 mM HEPES, 100 uM [2,3,3-2H,15N]-Leu beta 2 adrenergic receptor (N148C-MTSL), 1 mM ascorbate, 95% H2O/5% D2O | N148C-MTSL (diamagnetic) | 95% H2O/5% D2Omicelle7 | 20 mM HEPES, 100 uM [2,3,3-2H,15N]-Leu beta 2 adrenergic receptor (L266C-MTSL), 95% H2O/5% D2O | L266C-MTSL (paramagnetic) | 95% H2O/5% D2Omicelle8 | 20 mM HEPES, 100 uM [2,3,3-2H,15N]-Leu beta 2 adrenergic receptor (L266C-MTSL), 1 mM ascorbate, 95% H2O/5% D2O | L266C-MTSL (diamagnetic) | 95% H2O/5% D2Omicelle9 | 20 mM HEPES, 100 uM [2,3,3-2H,15N]-Leu beta 2 adrenergic receptor (I334C-MTSL), 95% H2O/5% D2O | I334C-MTSL (paramagnetic) | 95% H2O/5% D2Omicelle10 | 20 mM HEPES, 100 uM [2,3,3-2H,15N]-Leu beta 2 adrenergic receptor (I334C-MTSL), 1 mM ascorbate, 95% H2O/5% D2O | I334C-MTSL (diamagnetic) | 95% H2O/5% D2Omicelle11 | 20 mM HEPES, 100 uM [2,3,3-2H,15N]-Leu beta 2 adrenergic receptor (I334C-MTSL), 95% H2O/5% D2O | b2AR-D | 95% H2O/5% D2O | | | | | | | | | | | | | | | | | | | | | |
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Sample | Conc. (mg/ml) | Component | Isotopic labeling | Solution-ID |
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20 mMHEPESnatural abundance1 | 100 uMbeta 2 adrenergic receptor (A59C-MTSL) | [2,3,3-2H,15N]-Leu | 1 | 20 mMHEPESnatural abundance2 | 100 uMbeta 2 adrenergic receptor (A59C-MTSL) | [2,3,3-2H,15N]-Leu | 2 | 1 mMascorbatenatural abundance2 | 20 mMHEPESnatural abundance3 | 100 uMbeta 2 adrenergic receptor (T136C-MTSL) | [2,3,3-2H,15N]-Leu | 3 | 20 mMHEPESnatural abundance4 | 100 uMbeta 2 adrenergic receptor (T136C-MTSL) | [2,3,3-2H,15N]-Leu | 4 | 1 mMascorbatenatural abundance4 | 20 mMHEPESnatural abundance5 | 100 uMbeta 2 adrenergic receptor (N148C-MTSL) | [2,3,3-2H,15N]-Leu | 5 | 20 mMHEPESnatural abundance6 | 100 uMbeta 2 adrenergic receptor (N148C-MTSL) | [2,3,3-2H,15N]-Leu | 6 | 1 mMascorbatenatural abundance6 | 20 mMHEPESnatural abundance7 | 100 uMbeta 2 adrenergic receptor (L266C-MTSL) | [2,3,3-2H,15N]-Leu | 7 | 20 mMHEPESnatural abundance8 | 100 uMbeta 2 adrenergic receptor (L266C-MTSL) | [2,3,3-2H,15N]-Leu | 8 | 1 mMascorbatenatural abundance8 | 20 mMHEPESnatural abundance9 | 100 uMbeta 2 adrenergic receptor (I334C-MTSL) | [2,3,3-2H,15N]-Leu | 9 | 20 mMHEPES | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
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