Entry Database : PDB / ID : 6hep Structure visualization Downloads & linksTitle Crystal structure of human 14-3-3 beta in complex with CFTR R-domain peptide pS753-pS768 Components14-3-3 protein beta/alpha Cystic fibrosis transmembrane conductance regulator DetailsKeywords SIGNALING PROTEIN / 14-3-3 / CFTR / multivalencyFunction / homology Function and homology informationFunction Domain/homology Component
negative regulation of protein dephosphorylation / positive regulation of voltage-gated chloride channel activity / positive regulation of cyclic nucleotide-gated ion channel activity / Sec61 translocon complex binding / channel-conductance-controlling ATPase / intracellularly ATP-gated chloride channel activity / cytoplasmic sequestering of protein / positive regulation of enamel mineralization / transepithelial water transport / RHO GTPases regulate CFTR trafficking ... negative regulation of protein dephosphorylation / positive regulation of voltage-gated chloride channel activity / positive regulation of cyclic nucleotide-gated ion channel activity / Sec61 translocon complex binding / channel-conductance-controlling ATPase / intracellularly ATP-gated chloride channel activity / cytoplasmic sequestering of protein / positive regulation of enamel mineralization / transepithelial water transport / RHO GTPases regulate CFTR trafficking / intracellular pH elevation / Tristetraprolin (TTP, ZFP36) binds and destabilizes mRNA / ATPase-coupled inorganic anion transmembrane transporter activity / negative regulation of G protein-coupled receptor signaling pathway / amelogenesis / Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA / chloride channel inhibitor activity / Golgi-associated vesicle membrane / MTOR signalling / multicellular organismal-level water homeostasis / vesicle docking involved in exocytosis / ARMS-mediated activation / SHOC2 M1731 mutant abolishes MRAS complex function / Gain-of-function MRAS complexes activate RAF signaling / membrane hyperpolarization / cholesterol transport / Rap1 signalling / bicarbonate transport / bicarbonate transmembrane transporter activity / chloride channel regulator activity / Signaling by Hippo / vacuolar membrane / chloride transmembrane transporter activity / sperm capacitation / chloride channel activity / Frs2-mediated activation / protein kinase inhibitor activity / positive regulation of catalytic activity / cholesterol biosynthetic process / RHOQ GTPase cycle / mTORC1-mediated signalling / positive regulation of exocytosis / positive regulation of insulin secretion involved in cellular response to glucose stimulus / chloride channel complex / Regulation of localization of FOXO transcription factors / ATPase-coupled transmembrane transporter activity / phosphoserine residue binding / protein targeting / Activation of BAD and translocation to mitochondria / ABC-type transporter activity / SARS-CoV-2 targets host intracellular signalling and regulatory pathways / Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex / SARS-CoV-1 targets host intracellular signalling and regulatory pathways / cellular response to cAMP / RHO GTPases activate PKNs / cellular response to forskolin / isomerase activity / chloride transmembrane transport / response to endoplasmic reticulum stress / Translocation of SLC2A4 (GLUT4) to the plasma membrane / establishment of localization in cell / PDZ domain binding / TP53 Regulates Metabolic Genes / Defective CFTR causes cystic fibrosis / phosphoprotein binding / Late endosomal microautophagy / RAF activation / clathrin-coated endocytic vesicle membrane / Signaling by high-kinase activity BRAF mutants / MAP2K and MAPK activation / ABC-family proteins mediated transport / transmembrane transport / recycling endosome / Aggrephagy / Chaperone Mediated Autophagy / histone deacetylase binding / Negative regulation of MAPK pathway / recycling endosome membrane / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / Signaling by BRAF and RAF1 fusions / melanosome / Cargo recognition for clathrin-mediated endocytosis / Clathrin-mediated endocytosis / protein-folding chaperone binding / early endosome membrane / early endosome / endosome membrane / Ub-specific processing proteases / cadherin binding / apical plasma membrane / lysosomal membrane / protein domain specific binding / focal adhesion / endoplasmic reticulum membrane / perinuclear region of cytoplasm / enzyme binding / cell surface Similarity search - Function : / CFTR regulator domain / Cystic fibrosis TM conductance regulator (CFTR), regulator domain / Cystic fibrosis transmembrane conductance regulator / 14-3-3 domain / Delta-Endotoxin; domain 1 / 14-3-3 proteins signature 2. / 14-3-3 protein, conserved site / 14-3-3 proteins signature 1. / 14-3-3 protein ... : / CFTR regulator domain / Cystic fibrosis TM conductance regulator (CFTR), regulator domain / Cystic fibrosis transmembrane conductance regulator / 14-3-3 domain / Delta-Endotoxin; domain 1 / 14-3-3 proteins signature 2. / 14-3-3 protein, conserved site / 14-3-3 proteins signature 1. / 14-3-3 protein / 14-3-3 homologues / 14-3-3 domain / 14-3-3 domain superfamily / 14-3-3 protein / ABC transporter transmembrane region / ABC transporter type 1, transmembrane domain / ABC transporter integral membrane type-1 fused domain profile. / ABC transporter type 1, transmembrane domain superfamily / ABC transporter-like, conserved site / ABC transporters family signature. / ABC transporter / ABC transporter-like, ATP-binding domain / ATP-binding cassette, ABC transporter-type domain profile. / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Up-down Bundle / P-loop containing nucleoside triphosphate hydrolase / Mainly Alpha Similarity search - Domain/homologyBiological species Homo sapiens (human)Method X-RAY DIFFRACTION / SYNCHROTRON / Resolution : 1.86 Å DetailsAuthors Stevers, L.M. / Ottmann, C. / Brunsveld, L. Funding support Netherlands, 3items Details Hide detailsOrganization Grant number Country Netherlands Organisation for Scientific Research 024.001.035 Netherlands Netherlands Organisation for Scientific Research 2012 022.004.027 Netherlands Netherlands Organisation for Scientific Research 016.150.366 Netherlands
CitationJournal : J. Am. Chem. Soc. / Year : 2018Title : A Thermodynamic Model for Multivalency in 14-3-3 Protein-Protein Interactions.Authors : Stevers, L.M. / de Vink, P.J. / Ottmann, C. / Huskens, J. / Brunsveld, L. History Deposition Aug 20, 2018 Deposition site : PDBE / Processing site : PDBERevision 1.0 Oct 24, 2018 Provider : repository / Type : Initial releaseRevision 1.1 Nov 7, 2018 Group : Data collection / Database references / Category : citation / citation_authorItem : _citation.journal_volume / _citation.page_first ... _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.title / _citation_author.identifier_ORCID
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