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- PDB-6fzd: Crystal Structure of lipase from Geobacillus stearothermophilus T... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6fzd | ||||||
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Title | Crystal Structure of lipase from Geobacillus stearothermophilus T6 variant L184F/A187F/L360F | ||||||
![]() | Lipase![]() | ||||||
![]() | ![]() ![]() ![]() ![]() | ||||||
Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gihaz, S. / Kanteev, M. / Pazy, Y. / Fishman, A. | ||||||
![]() | ![]() Title: Filling the Void: Introducing Aromatic Interactions into Solvent Tunnels To Enhance Lipase Stability in Methanol. Authors: Gihaz, S. / Kanteev, M. / Pazy, Y. / Fishman, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 177.8 KB | Display | ![]() |
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PDB format | ![]() | 139.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6fz1C ![]() 6fz7C ![]() 6fz8C ![]() 6fz9C ![]() 6fzaC ![]() 6fzcC ![]() 4x6uS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 44077.129 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Production host: ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-ZN / |
#3: Chemical | ChemComp-CA / |
#4: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 45.78 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: 0.2M sodium citrate, 25% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 4M / Detector: PIXEL / Date: Dec 9, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.8→45.63 Å / Num. obs: 37550 / % possible obs: 98.8 % / Redundancy: 7.9 % / CC1/2: 1 / Rmerge(I) obs: 0.048 / Rrim(I) all: 0.051 / Net I/σ(I): 35.3 |
Reflection shell | Resolution: 1.8→1.8487 Å |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 4X6U Resolution: 1.8→45.628 Å / SU ML: 0.17 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 21.06
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→45.628 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -10.4492 Å / Origin y: 9.734 Å / Origin z: -18.177 Å
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Refinement TLS group | Selection details: all |