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- PDB-6fz7: Crystal Structure of lipase from Geobacillus stearothermophilus T... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6fz7 | ||||||
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Title | Crystal Structure of lipase from Geobacillus stearothermophilus T6 methanol stable variant L184F | ||||||
![]() | Lipase![]() | ||||||
![]() | ![]() ![]() ![]() ![]() | ||||||
Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gihaz, S. / Kanteev, M. / Pazy, Y. / Fishman, A. | ||||||
![]() | ![]() Title: Filling the Void: Introducing Aromatic Interactions into Solvent Tunnels To Enhance Lipase Stability in Methanol. Authors: Gihaz, S. / Kanteev, M. / Pazy, Y. / Fishman, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 180.8 KB | Display | ![]() |
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PDB format | ![]() | 141.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6fz1C ![]() 6fz8C ![]() 6fz9C ![]() 6fzaC ![]() 6fzcC ![]() 6fzdC ![]() 4x6uS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 43967.016 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Production host: ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-ZN / |
#3: Chemical | ChemComp-CA / |
#4: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.33 Å3/Da / Density % sol: 47.26 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: 0.2M sodium citrate, 25% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: May 20, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.736→57.255 Å / Num. obs: 43045 / % possible obs: 99.4 % / Redundancy: 5.4 % / CC1/2: 0.997 / Rmerge(I) obs: 0.08 / Rrim(I) all: 0.09 / Net I/σ(I): 11.8 |
Reflection shell | Resolution: 1.736→1.736 Å |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 4X6U Resolution: 1.736→57.255 Å / SU ML: 0.11 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 18.86
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.736→57.255 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: -10.5673 Å / Origin y: 11.0237 Å / Origin z: -18.5546 Å
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Refinement TLS group | Selection details: all |