+Open data
-Basic information
Entry | Database: PDB / ID: 6fsp | ||||||
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Title | Crystal structure of APRT from Thermus thermophilus | ||||||
Components | PRPP-binding protein, adenine/guanine phosphoribosyltransferase | ||||||
Keywords | TRANSFERASE | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Thermus thermophilus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.7 Å | ||||||
Authors | Timofeev, V.I. / Sinitsyna, E.V. / Kostromina, M.A. / Muravieva, T.I. / Makarov, D.A. / Mikheeva, O.O. / Kuranova, I.P. / Esipov, R.S. | ||||||
Citation | Journal: To Be Published Title: Crystal structure of APRT from Thermus thermophilus Authors: Timofeev, V.I. / Sinitsyna, E.V. / Kostromina, M.A. / Muravieva, T.I. / Makarov, D.A. / Mikheeva, O.O. / Kuranova, I.P. / Esipov, R.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6fsp.cif.gz | 384.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6fsp.ent.gz | 320.3 KB | Display | PDB format |
PDBx/mmJSON format | 6fsp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fs/6fsp ftp://data.pdbj.org/pub/pdb/validation_reports/fs/6fsp | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 18425.527 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Details: TYPVEIAGVRRELPIVQVGPGVAVALLNLLGDTELTEAAAEALAKRLPPEVEVLVTPEVKAVPLAHALSRITGKPYVVARKTEKPYMINPVSRQVLSITTGKPQLLVLDGADIPRVRGKKVAIVDDVVSTGSTLAGLRELIESVGGEVVAVLAVFTEGTPRQDVVALGHLP Source: (gene. exp.) Thermus thermophilus (bacteria) / Gene: TtJL18_0431 / Production host: Escherichia coli (E. coli) / References: UniProt: H9ZPT0, UniProt: Q5SHW6*PLUS |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.33 Å3/Da / Density % sol: 47.2 % |
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Crystal grow | Temperature: 296 K / Method: liquid diffusion / Details: SA 25% |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL41XU / Wavelength: 0.8 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Dec 8, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→20 Å / Num. obs: 25557 / % possible obs: 96.6 % / Redundancy: 3.4 % / Net I/σ(I): 15.24 |
Reflection shell | Resolution: 2.7→2.77 Å |
-Processing
Software |
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Refinement | Resolution: 2.7→20 Å / Cor.coef. Fo:Fc: 0.85 / Cor.coef. Fo:Fc free: 0.77 / SU B: 50.631 / SU ML: 0.483 / Cross valid method: THROUGHOUT / ESU R Free: 0.556 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 46.147 Å2
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Refinement step | Cycle: 1 / Resolution: 2.7→20 Å
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Refine LS restraints |
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