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- PDB-6edv: Structure of a GNAT superfamily acetyltransferase PA3944 in compl... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6edv | ||||||
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Title | Structure of a GNAT superfamily acetyltransferase PA3944 in complex with CoA | ||||||
![]() | Acetyltransferase PA3944 | ||||||
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Function / homology | Acetyltransferase (GNAT) domain / acyltransferase activity, transferring groups other than amino-acyl groups / Gcn5-related N-acetyltransferase (GNAT) domain profile. / ![]() ![]() ![]() ![]() | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Majorek, K.A. / Satchell, K.J.F. / Joachimiak, A. / Minor, W. / Center for Structural Genomics of Infectious Diseases (CSGID) | ||||||
![]() | ![]() Title: A Gcn5-Related N-Acetyltransferase (GNAT) Capable of Acetylating Polymyxin B and Colistin Antibiotics in Vitro. Authors: Czub, M.P. / Zhang, B. / Chiarelli, M.P. / Majorek, K.A. / Joe, L. / Porebski, P.J. / Revilla, A. / Wu, W. / Becker, D.P. / Minor, W. / Kuhn, M.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 99.7 KB | Display | ![]() |
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PDB format | ![]() | 73.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6eddC ![]() 3fbuS C: citing same article ( S: Starting model for refinement |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 22080.045 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 Gene: PA3944 / Production host: ![]() ![]() ![]() References: UniProt: Q9HX72, ![]() | ||||
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#2: Chemical | ChemComp-CA / | ||||
#3: Chemical | ![]() #4: Chemical | ChemComp-COA / | ![]() #5: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 39.09 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 100 mM Tris-HCl pH 7.0, 200 mM calcium acetate monohydrate, 20%w/v PEG 3000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Mar 3, 2012 / Details: mirrors |
Radiation | Monochromator: Si 111 channel / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.35→30 Å / Num. obs: 40378 / % possible obs: 100 % / Redundancy: 13.8 % / Rmerge(I) obs: 0.122 / Net I/σ(I): 7.9 |
Reflection shell | Resolution: 1.35→1.37 Å / Redundancy: 4.6 % / Rmerge(I) obs: 0.778 / % possible all: 99.7 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 3FBU Resolution: 1.35→30 Å / SU B: 1.457 / SU ML: 0.029 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.05 / ESU R Free: 0.047 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING
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Displacement parameters | Biso mean: 18.45 Å2
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Refinement step | Cycle: LAST / Resolution: 1.35→30 Å
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