根拠: light scattering, Multi-angle light scattering experiments (and others) indicate that sigma-1 recepto can adopt a variety of oligomerization states. The trimeric arrangement present in the ...根拠: light scattering, Multi-angle light scattering experiments (and others) indicate that sigma-1 recepto can adopt a variety of oligomerization states. The trimeric arrangement present in the asymmetric unit shown here is likely representative of one particular biological oligomer.
タイプ
名称
対称操作
数
identity operation
1_555
x,y,z
1
Buried area
8950 Å2
ΔGint
-174 kcal/mol
Surface area
31250 Å2
手法
PISA
単位格子
Length a, b, c (Å)
84.837, 128.590, 109.180
Angle α, β, γ (deg.)
90.00, 90.00, 90.00
Int Tables number
18
Space group name H-M
P21212
Components on special symmetry positions
ID
モデル
要素
1
1
C-304-
SO4
-
要素
-
タンパク質 , 1種, 3分子 ABC
#1: タンパク質
Sigmanon-opioidintracellularreceptor1 / Aging-associated gene 8 protein / SR31747-binding protein / SR-BP / Sigma 1-type opioid receptor / hSigmaR1
温度: 293 K / 手法: 脂質キュービック相法 / pH: 6 詳細: Protein was reconstituted in cubic phase by mixing with a 1:1.5 (w:w) protein:lipid ratio. Lipid used was a 10:1 (w:w) mix of monoolein with cholesterol. The crystal was grown in 240 mM ...詳細: Protein was reconstituted in cubic phase by mixing with a 1:1.5 (w:w) protein:lipid ratio. Lipid used was a 10:1 (w:w) mix of monoolein with cholesterol. The crystal was grown in 240 mM lithium sulfate, 42% PEG 300, 1% hexanediol, and 0.1M MES pH 6.0