+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 6cnk | ||||||||||||
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タイトル | Structure of the 3alpha2beta stiochiometry of the human Alpha4Beta2 nicotinic receptor | ||||||||||||
要素 |
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キーワード | TRANSPORT PROTEIN (運搬体タンパク質) / Ligand-gated ion channel (リガンド依存性イオンチャネル) / Acetylcholine receptor (アセチルコリン受容体) / Cys-loop Receptor / MEMBRANE PROTEIN (膜タンパク質) | ||||||||||||
機能・相同性 | 機能・相同性情報 vestibulocochlear nerve development / lateral geniculate nucleus development / regulation of circadian sleep/wake cycle, REM sleep / regulation of synaptic transmission, dopaminergic / quaternary ammonium group binding / synaptic transmission involved in micturition / Highly sodium permeable postsynaptic acetylcholine nicotinic receptors / Highly calcium permeable nicotinic acetylcholine receptors / optic nerve morphogenesis / response to acetylcholine ...vestibulocochlear nerve development / lateral geniculate nucleus development / regulation of circadian sleep/wake cycle, REM sleep / regulation of synaptic transmission, dopaminergic / quaternary ammonium group binding / synaptic transmission involved in micturition / Highly sodium permeable postsynaptic acetylcholine nicotinic receptors / Highly calcium permeable nicotinic acetylcholine receptors / optic nerve morphogenesis / response to acetylcholine / Highly calcium permeable postsynaptic nicotinic acetylcholine receptors / central nervous system projection neuron axonogenesis / acetylcholine-gated channel complex / regulation of dopamine metabolic process / cholinergic synapse / negative regulation of action potential / behavioral response to nicotine / positive regulation of dopamine secretion / acetylcholine receptor activity / postsynaptic specialization membrane / inhibitory postsynaptic potential / nervous system process / synaptic transmission, cholinergic / acetylcholine binding / acetylcholine receptor signaling pathway / acetylcholine-gated monoatomic cation-selective channel activity / regulation of synapse assembly / 活動電位 / regulation of dendrite morphogenesis / heterocyclic compound binding / regulation of dopamine secretion / B cell activation / plasma membrane raft / 脱分極 / social behavior / 学習 / ligand-gated monoatomic ion channel activity / smooth muscle contraction / monoatomic ion transport / positive regulation of B cell proliferation / sensory perception of pain / 視覚 / locomotory behavior / response to cocaine / 学習 / regulation of membrane potential / sensory perception of sound / response to nicotine / visual learning / 記憶 / 認識 / calcium ion transport / presynaptic membrane / chemical synaptic transmission / postsynaptic membrane / response to ethanol / response to oxidative stress / response to hypoxia / neuron projection / external side of plasma membrane / DNA修復 / neuronal cell body / シナプス / 樹状突起 / protein-containing complex binding / シグナル伝達 / 生体膜 / 細胞膜 類似検索 - 分子機能 | ||||||||||||
生物種 | Homo sapiens (ヒト) Mus musculus (ハツカネズミ) | ||||||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.9 Å | ||||||||||||
データ登録者 | Walsh Jr, R.M. / Roh, S.H. / Gharpure, A. / Morales-Perez, C.L. / Hibbs, R.E. | ||||||||||||
資金援助 | 米国, 3件
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引用 | ジャーナル: Nature / 年: 2018 タイトル: Structural principles of distinct assemblies of the human α4β2 nicotinic receptor. 著者: Richard M Walsh / Soung-Hun Roh / Anant Gharpure / Claudio L Morales-Perez / Jinfeng Teng / Ryan E Hibbs / 要旨: Fast chemical communication in the nervous system is mediated by neurotransmitter-gated ion channels. The prototypical member of this class of cell surface receptors is the cation-selective nicotinic ...Fast chemical communication in the nervous system is mediated by neurotransmitter-gated ion channels. The prototypical member of this class of cell surface receptors is the cation-selective nicotinic acetylcholine receptor. As with most ligand-gated ion channels, nicotinic receptors assemble as oligomers of subunits, usually as hetero-oligomers and often with variable stoichiometries . This intrinsic heterogeneity in protein composition provides fine tunability in channel properties, which is essential to brain function, but frustrates structural and biophysical characterization. The α4β2 subtype of the nicotinic acetylcholine receptor is the most abundant isoform in the human brain and is the principal target in nicotine addiction. This pentameric ligand-gated ion channel assembles in two stoichiometries of α- and β-subunits (2α:3β and 3α:2β). Both assemblies are functional and have distinct biophysical properties, and an imbalance in the ratio of assemblies is linked to both nicotine addiction and congenital epilepsy. Here we leverage cryo-electron microscopy to obtain structures of both receptor assemblies from a single sample. Antibody fragments specific to β2 were used to 'break' symmetry during particle alignment and to obtain high-resolution reconstructions of receptors of both stoichiometries in complex with nicotine. The results reveal principles of subunit assembly and the structural basis of the distinctive biophysical and pharmacological properties of the two different stoichiometries of this receptor. | ||||||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 6cnk.cif.gz | 491.7 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb6cnk.ent.gz | 404.1 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 6cnk.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/cn/6cnk ftp://data.pdbj.org/pub/pdb/validation_reports/cn/6cnk | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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-要素
-Neuronal acetylcholine receptor subunit ... , 2種, 5分子 ABDCE
#1: タンパク質 | 分子量: 44862.367 Da / 分子数: 3 変異: Glu-Arg linker was inserted in the MX-M4 junction, between Phe559-Ser560 in the alpha4 subunit,Glu-Arg linker was inserted in the MX-M4 junction, between Phe559-Ser560 in the alpha4 subunit 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: CHRNA4, NACRA4 / プラスミド: pEZT-BM / 細胞株 (発現宿主): HEK293 / 発現宿主: Homo sapiens (ヒト) / Variant (発現宿主): GnTI- / 参照: UniProt: P43681 #2: タンパク質 | 分子量: 46748.863 Da / 分子数: 2 変異: Glu-Arg linker was inserted in the MX-M4 junction between Gln420-Ser421 in the beta 2 subunit.,Glu-Arg linker was inserted in the MX-M4 junction between Gln420-Ser421 in the beta 2 subunit. 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: CHRNB2 / プラスミド: pEZT-BM / 細胞株 (発現宿主): HEK293 / 発現宿主: Homo sapiens (ヒト) / Variant (発現宿主): GnTI- / 参照: UniProt: P17787 |
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-抗体 , 2種, 4分子 FJGK
#3: 抗体 | 分子量: 26378.596 Da / 分子数: 2 / 由来タイプ: 天然 / 由来: (天然) Mus musculus (ハツカネズミ) / Plasmid details: From generated Hybridoma / 株: BALB/c #4: 抗体 | 分子量: 51195.668 Da / 分子数: 2 / 由来タイプ: 天然 / 由来: (天然) Mus musculus (ハツカネズミ) / Plasmid details: From generated Hybridoma / 株: BALB/c |
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-糖 , 2種, 5分子
#5: 多糖 | #6: 糖 | |
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-非ポリマー , 3種, 14分子
#7: 化合物 | ChemComp-Y01 / #8: 化合物 | ChemComp-NA / | #9: 化合物 | |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-試料調製
構成要素 | 名称: Complex of two Fab fragments with the 3alpha2beta stoichiometry of the human Alpha4Beta2 nicotinic receptor タイプ: COMPLEX 詳細: Fab fragmented generated by proteolytic cleavage of IgG antibody Entity ID: #1-#4 / 由来: MULTIPLE SOURCES | ||||||||||||||||||||||||||||||
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分子量 | 値: 0.294 MDa | ||||||||||||||||||||||||||||||
由来(天然) | 生物種: Homo sapiens (ヒト) | ||||||||||||||||||||||||||||||
緩衝液 | pH: 7.4 | ||||||||||||||||||||||||||||||
緩衝液成分 |
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試料 | 濃度: 6 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES | ||||||||||||||||||||||||||||||
試料支持 | 詳細: Sample was glow discharged at 30mA for 80 seconds using a PELCO easiGLow グリッドの材料: GOLD / グリッドのサイズ: 200 divisions/in. / グリッドのタイプ: Quantifoil R1.2/1.3 | ||||||||||||||||||||||||||||||
急速凍結 | 装置: FEI VITROBOT MARK IV / 凍結剤: ETHANE / 湿度: 100 % / 凍結前の試料温度: 277 K / 詳細: 4 second blot time |
-電子顕微鏡撮影
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM |
電子レンズ | モード: BRIGHT FIELDBright-field microscopy / 倍率(補正後): 46730 X / 最大 デフォーカス(公称値): 4500 nm / 最小 デフォーカス(公称値): 500 nm / Cs: 2.7 mm / C2レンズ絞り径: 70 µm / アライメント法: COMA FREE |
試料ホルダ | 凍結剤: NITROGEN 試料ホルダーモデル: FEI TITAN KRIOS AUTOGRID HOLDER 最高温度: 90 K / 最低温度: 80 K |
撮影 | 平均露光時間: 15 sec. / 電子線照射量: 75 e/Å2 / 検出モード: SUPER-RESOLUTION フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) 撮影したグリッド数: 2 / 実像数: 5166 |
電子光学装置 | エネルギーフィルター名称: GIF Quantum LS / エネルギーフィルター 上限: 10 eV / エネルギーフィルター 下限: -10 eV |
-解析
EMソフトウェア |
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||||||||||||||||||
粒子像の選択 | 選択した粒子像数: 649773 | ||||||||||||||||||||||||||||||||||||||||
対称性 | 点対称性: C1 (非対称) | ||||||||||||||||||||||||||||||||||||||||
3次元再構成 | 解像度: 3.9 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 139551 / アルゴリズム: BACK PROJECTION / クラス平均像の数: 2 / 対称性のタイプ: POINT | ||||||||||||||||||||||||||||||||||||||||
原子モデル構築 | プロトコル: AB INITIO MODEL / 空間: REAL | ||||||||||||||||||||||||||||||||||||||||
精密化 | 最高解像度: 3.7 Å |