+Open data
-Basic information
Entry | Database: PDB / ID: 6c2r | ||||||
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Title | Aurora A ligand complex | ||||||
Components | Aurora kinase A | ||||||
Keywords | TRANSFERASE / Protein kinase | ||||||
Function / homology | Function and homology information Interaction between PHLDA1 and AURKA / regulation of centrosome cycle / axon hillock / spindle assembly involved in female meiosis I / cilium disassembly / positive regulation of oocyte maturation / spindle pole centrosome / histone H3S10 kinase activity / chromosome passenger complex / pronucleus ...Interaction between PHLDA1 and AURKA / regulation of centrosome cycle / axon hillock / spindle assembly involved in female meiosis I / cilium disassembly / positive regulation of oocyte maturation / spindle pole centrosome / histone H3S10 kinase activity / chromosome passenger complex / pronucleus / meiotic spindle / mitotic centrosome separation / germinal vesicle / protein localization to centrosome / anterior/posterior axis specification / centrosome localization / neuron projection extension / spindle organization / positive regulation of mitochondrial fission / mitotic spindle pole / SUMOylation of DNA replication proteins / spindle midzone / regulation of G2/M transition of mitotic cell cycle / centriole / protein serine/threonine/tyrosine kinase activity / positive regulation of mitotic cell cycle / positive regulation of mitotic nuclear division / AURKA Activation by TPX2 / TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest / mitotic spindle organization / ciliary basal body / regulation of cytokinesis / negative regulation of protein binding / regulation of signal transduction by p53 class mediator / molecular function activator activity / liver regeneration / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 / regulation of protein stability / spindle microtubule / mitotic spindle / kinetochore / response to wounding / spindle / G2/M transition of mitotic cell cycle / microtubule cytoskeleton / Regulation of PLK1 Activity at G2/M Transition / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / mitotic cell cycle / midbody / basolateral plasma membrane / peptidyl-serine phosphorylation / proteasome-mediated ubiquitin-dependent protein catabolic process / Regulation of TP53 Activity through Phosphorylation / protein autophosphorylation / postsynaptic density / non-specific serine/threonine protein kinase / protein kinase activity / protein heterodimerization activity / cell division / protein phosphorylation / negative regulation of gene expression / protein serine kinase activity / protein serine/threonine kinase activity / centrosome / glutamatergic synapse / apoptotic process / ubiquitin protein ligase binding / negative regulation of apoptotic process / protein kinase binding / perinuclear region of cytoplasm / nucleoplasm / ATP binding / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.96 Å | ||||||
Authors | Antonysamy, S. / Pustilnik, A. / Manglicmot, D. / Froning, K. / Weichert, K. / Wasserman, S. | ||||||
Citation | Journal: Cancer Discov / Year: 2019 Title: Aurora A Kinase Inhibition Is Synthetic Lethal with Loss of theRB1Tumor Suppressor Gene. Authors: Gong, X. / Du, J. / Parsons, S.H. / Merzoug, F.F. / Webster, Y. / Iversen, P.W. / Chio, L.C. / Van Horn, R.D. / Lin, X. / Blosser, W. / Han, B. / Jin, S. / Yao, S. / Bian, H. / Ficklin, C. / ...Authors: Gong, X. / Du, J. / Parsons, S.H. / Merzoug, F.F. / Webster, Y. / Iversen, P.W. / Chio, L.C. / Van Horn, R.D. / Lin, X. / Blosser, W. / Han, B. / Jin, S. / Yao, S. / Bian, H. / Ficklin, C. / Fan, L. / Kapoor, A. / Antonysamy, S. / Mc Nulty, A.M. / Froning, K. / Manglicmot, D. / Pustilnik, A. / Weichert, K. / Wasserman, S.R. / Dowless, M. / Marugan, C. / Baquero, C. / Lallena, M.J. / Eastman, S.W. / Hui, Y.H. / Dieter, M.Z. / Doman, T. / Chu, S. / Qian, H.R. / Ye, X.S. / Barda, D.A. / Plowman, G.D. / Reinhard, C. / Campbell, R.M. / Henry, J.R. / Buchanan, S.G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6c2r.cif.gz | 125 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6c2r.ent.gz | 96.1 KB | Display | PDB format |
PDBx/mmJSON format | 6c2r.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/c2/6c2r ftp://data.pdbj.org/pub/pdb/validation_reports/c2/6c2r | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 31440.150 Da / Num. of mol.: 1 / Fragment: residues 125-391 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) Gene: AURKA, AIK, AIRK1, ARK1, AURA, AYK1, BTAK, IAK1, STK15, STK6 Production host: unidentified baculovirus References: UniProt: O14965, non-specific serine/threonine protein kinase | ||||
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#2: Chemical | #3: Chemical | ChemComp-EG7 / ( | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.12 Å3/Da / Density % sol: 41.87 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, sitting drop / pH: 4.6 Details: 100mM MES pH 4.6, 23% PEG 3350, 150mM Ammonium Sulfate |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 31-ID / Wavelength: 0.97931 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: May 29, 2014 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.97931 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 1.96→19.804 Å / Num. obs: 19961 / % possible obs: 99.9 % / Redundancy: 13.8 % / Biso Wilson estimate: 31.24 Å2 / Rmerge(I) obs: 0.141 / Rrim(I) all: 0.149 / Rsym value: 0.141 / Net I/av σ(I): 3.9 / Net I/σ(I): 10.9 / Num. measured all: 275445 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Processing
Software |
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Refinement | Resolution: 1.96→19.7 Å / Cor.coef. Fo:Fc: 0.952 / Cor.coef. Fo:Fc free: 0.923 / SU R Cruickshank DPI: 0.16 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.164 / SU Rfree Blow DPI: 0.149 / SU Rfree Cruickshank DPI: 0.148
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Displacement parameters | Biso max: 118.46 Å2 / Biso mean: 41.64 Å2 / Biso min: 15.55 Å2
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Refine analyze | Luzzati coordinate error obs: 0.26 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.96→19.7 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.96→2.07 Å / Rfactor Rfree error: 0 / Total num. of bins used: 10
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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