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Open data
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Basic information
Entry | Database: PDB / ID: 6bc7 | ||||||
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Title | Cryo X-ray structure of sisomicin bound AAC-VIa | ||||||
![]() | AAC 3-VI protein | ||||||
![]() | TRANSFERASE/ANTIBIOTIC / ![]() ![]() | ||||||
Function / homology | ![]() aminoglycoside 3-N-acetyltransferase / aminoglycoside 3-N-acetyltransferase activity / response to antibiotic / ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Cuneo, M.J. / Kumar, P. | ||||||
![]() | ![]() Title: A low-barrier hydrogen bond mediates antibiotic resistance in a noncanonical catalytic triad. Authors: Kumar, P. / Serpersu, E.H. / Cuneo, M.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 78.7 KB | Display | ![]() |
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PDB format | ![]() | 56 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6bbrC ![]() 6bbzC ![]() 6bc2C ![]() 6bc3C ![]() 6bc4C ![]() 6bc5C ![]() 6bc6SC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 32344.461 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-SIS / (![]() |
#3: Chemical | ChemComp-ACT / ![]() |
#4: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.3 Å3/Da / Density % sol: 46.57 % |
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Crystal grow![]() | Temperature: 285 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: 17-25% PEG4000, 0.1 M sodium citrate, pH 5.6, 0.2 M ammonium acetate, 10% glycerol |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Jul 10, 2016 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength![]() | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 1.8→50 Å / Num. obs: 26880 / % possible obs: 98.7 % / Redundancy: 2.9 % / Biso Wilson estimate: 16.54 Å2 / Rmerge(I) obs: 0.089 / Χ2: 1.258 / Net I/σ(I): 9.1 / Num. measured all: 76779 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB entry 6BC6 Resolution: 1.8→35.615 Å / SU ML: 0.19 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 17.46 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→35.615 Å
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Refine LS restraints |
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LS refinement shell |
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