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- PDB-5xns: Crystal structure of the Smc head domain with an extended coiled ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5xns | ||||||
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Title | Crystal structure of the Smc head domain with an extended coiled coil bound to the C-terminal domain of ScpA derived from Pyrococcus furiosus | ||||||
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![]() | DNA BINDING PROTEIN/CELL CYCLE / ![]() ![]() | ||||||
Function / homology | ![]() cohesin loader activity / chromosome condensation / mitotic sister chromatid cohesion / ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kwak, M.-J. / Shin, H.-C. / Oh, B.-H. | ||||||
![]() | ![]() Title: Structure of Full-Length SMC and Rearrangements Required for Chromosome Organization Authors: Diebold-Durand, M.L. / Lee, H. / Ruiz Avila, L.B. / Noh, H. / Shin, H.-C. / Im, H. / Bock, F.P. / Burmann, F. / Durand, A. / Basfeld, A. / Ham, S. / Basquin, J. / Oh, B.-H. / Gruber, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 108.1 KB | Display | ![]() |
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PDB format | ![]() | 80.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5nmoC ![]() 5nnvC ![]() 5xeiC ![]() 5xg2C ![]() 5xg3C ![]() 4i99S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 45220.027 Da / Num. of mol.: 1 Fragment: UNP residues 1-201 and 973-1069, linked with linker residues SGGSGGS Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1 / Gene: smc, PF1843 / Production host: ![]() ![]() ![]() | ||
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#2: Protein | Mass: 8783.374 Da / Num. of mol.: 1 / Fragment: UNP residues 143-212 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1 / Gene: PF1842 / Production host: ![]() ![]() ![]() | ||
#3: Chemical | ![]() #4: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.37 Å3/Da / Density % sol: 63.49 % |
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Crystal grow![]() | Temperature: 295 K / Method: vapor diffusion, hanging drop Details: 1M Ammonium citrate dibasic, 0.1M Sodium acetate trihydrate pH 4.6, 0.3M NDSB-195 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Oct 31, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.01→50 Å / Num. obs: 45242 / % possible obs: 92.8 % / Redundancy: 6 % / Biso Wilson estimate: 27.06 Å2 / Net I/σ(I): 31.6 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 4I99 Resolution: 2.01→42.932 Å / SU ML: 0.26 / Cross valid method: FREE R-VALUE / σ(F): 1.66 / Phase error: 23.56
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 77.73 Å2 / Biso mean: 34.2864 Å2 / Biso min: 13.78 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.01→42.932 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 14
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