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- PDB-5upl: CDC42 binds PAK4 via an extended GTPase-effector inteface - 2 pep... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5upl | ||||||
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Title | CDC42 binds PAK4 via an extended GTPase-effector inteface - 2 peptide: PAK4FL, CDC42 - UNREFINED | ||||||
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Function / homology | ![]() GBD domain binding / submandibular salivary gland formation / actin filament branching / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ha, B.H. / Boggon, T.J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: CDC42 binds PAK4 via an extended GTPase-effector interface. Authors: Ha, B.H. / Boggon, T.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 104.8 KB | Display | ![]() |
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PDB format | ![]() | 75.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5upkC ![]() 4fieS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 51099.684 Da / Num. of mol.: 1 / Fragment: UNP residues 2-426 / Mutation: S474SEP Source method: isolated from a genetically manipulated source Details: Ser474 is phosphorylated (SEP) / Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: UniProt: O96013, ![]() |
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#2: Protein | Mass: 20716.744 Da / Num. of mol.: 1 / Fragment: UNP residues 1-177 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50.88 % |
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Crystal grow![]() | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 8.5 / Details: 0.1M Tris-HCl pH 8.5, 50mM Na2SO4, 6% PEG6000 / PH range: 8.0-9.0 / Temp details: R/T |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Dec 9, 2013 |
Radiation | Monochromator: Si (111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 3→43.637 Å / Num. obs: 14433 / % possible obs: 99.9 % / Redundancy: 18 % / CC1/2: 0.588 / Rmerge(I) obs: 0.117 / Rpim(I) all: 0.034 / Rsym value: 0.117 / Χ2: 1.187 / Net I/σ(I): 20.3 |
Reflection shell | Resolution: 3→3.11 Å / Mean I/σ(I) obs: 1.6 / CC1/2: 0.588 / Rpim(I) all: 0.707 / Χ2: 1.608 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 4fie Resolution: 3.003→43.637 Å / SU ML: 0.57 / Cross valid method: NONE / σ(F): 1.33 / Phase error: 34.46 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.003→43.637 Å
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Refine LS restraints |
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LS refinement shell |
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