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- PDB-5udo: Crystal structure of the coiled-coil domain from Listeria Innocua... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5udo | ||||||
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Title | Crystal structure of the coiled-coil domain from Listeria Innocua Phage Integrase (Tetragonal Form II) | ||||||
![]() | A118 serine integrase | ||||||
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Function / homology | ![]() DNA strand exchange activity / DNA integration / ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gupta, K. / Yuan, J.B. / Sharp, R. / Van Duyne, G.D. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Coiled-coil interactions mediate serine integrase directionality. Authors: Gupta, K. / Sharp, R. / Yuan, J.B. / Li, H. / Van Duyne, G.D. #1: ![]() Title: Attachment site recognition and regulation of directionality by the serine integrases. Authors: Rutherford, K. / Yuan, P. / Perry, K. / Sharp, R. / Van Duyne, G.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 138.1 KB | Display | ![]() |
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PDB format | ![]() | 91.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5u96C ![]() 5uaeC ![]() 4kisS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 39155.633 Da / Num. of mol.: 8 / Fragment: coiled-coil domain (UNP residues 133-452) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal grow![]() | Temperature: 294.16 K / Method: vapor diffusion, hanging drop / pH: 7.5 / Details: 0.8-1.2 M Na Citrate |
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 2M / Detector: PIXEL / Date: Jun 3, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.541→50 Å / Num. obs: 16440 / % possible obs: 98.7 % / Redundancy: 5.5 % / Biso Wilson estimate: 67 Å2 / Rmerge(I) obs: 0.091 / Net I/σ(I): 12.9 |
Reflection shell | Resolution: 2.541→2.64 Å / Rmerge(I) obs: 0.615 / Mean I/σ(I) obs: 1.95 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 4KIS Resolution: 2.541→35.853 Å / Cross valid method: FREE R-VALUE / σ(F): 1.38 / Phase error: 32.79 / Stereochemistry target values: TWIN_LSQ_F
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.541→35.853 Å
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Refine LS restraints |
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LS refinement shell |
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