PDB-5u2w: Crystal structure of a Short chain dehydrogenase from Burkholderi...

ID or keywords:

Entry

Database: PDB / ID: 5u2w

Title

Crystal structure of a Short chain dehydrogenase from Burkholderia cenocepacia J2315 in complex with NADP

Components

Short chain dehydrogenase

Keywords

OXIDOREDUCTASE /

Structural Genomics / Seattle Structural Genomics Center for Infectious Disease / SSGCID

Function / homology

Function and homology information

: /

nucleotide binding
Similarity search - Function

Biological species

Burkholderia cenocepacia (bacteria)

Method

X-RAY DIFFRACTION /

MOLECULAR REPLACEMENT /

molecular replacement / Resolution: 1.55 Å

Authors

Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Citation

Journal: to be published
Title: Crystal structure of a Short chain dehydrogenase from Burkholderia cenocepacia J2315 in complex with NADP
Authors: Abendroth, J. / Horanyi, P.S. / Lorimer, D.D. / Edwards, T.E.

History

Deposition	Dec 1, 2016	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Jan 18, 2017	Provider: repository / Type: Initial release
Revision 1.1	Oct 4, 2023	Group: Data collection / Database references / Refinement description Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	5u2w.cif.gz	397.1 KB	Display	PDBx/mmCIF format
PDB format	pdb5u2w.ent.gz	322.6 KB	Display	PDB format
PDBx/mmJSON format	5u2w.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/u2/5u2w ftp://data.pdbj.org/pub/pdb/validation_reports/u2/5u2w	HTTPS FTP

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Related structure data

Related structure data	5u4sC 3v2gS S: Starting model for refinement C: citing same article (ref.)
Similar structure data	6y0z-1 3nrc-1 4ibo-d 5t5q-d 5er6-d 5ha5-d 4d0s-d 5wuw-1 4cql-1 6le3-d Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search
Other databases	TargetTrack: SSGCID-BuceA.00010.g

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#188 - Aug 2015 Tetrahydrobiopterin Biosynthesis similarity (1) #216 - Dec 2017 Biodegradable Plastic similarity (1) #134 - Feb 2011 Integrin similarity (1) #172 - Apr 2014 RecA and Rad51 similarity (1) #194 - Feb 2016 Designer Insulins similarity (1) #234 - Jun 2019 MDM2 and Cancer similarity (1) #259 - Jul 2021 Designed Proteins and Citizen Science similarity (1)

Deposited unit

A: Short chain dehydrogenase

B: Short chain dehydrogenase

C: Short chain dehydrogenase

D: Short chain dehydrogenase

hetero molecules

108 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

Idetical with deposited unit
defined by author&software

Unit cell

Length a, b, c (Å)	136.970, 72.370, 100.530
Angle α, β, γ (deg.)	90.000, 100.880, 90.000
Int Tables number	5
Space group name H-M	C121

Components on special symmetry positions

ID	Model	Components
1	1	B-407- HOH
2	1	C-535- HOH

#1: Protein	Short chain dehydrogenase Mass: 26019.244 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (bacteria) Strain: ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610 Gene: BCAL2464 / Plasmid: BuceA.00010.g.B1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: B4E730
#2: Chemical	ChemComp-NAP / NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / 2'-MONOPHOSPHOADENOSINE 5'-DIPHOSPHORIBOSE / Nicotinamide adenine dinucleotide phosphate Mass: 743.405 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₂₁H₂₈N₇O₁₇P₃
#3: Chemical	ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol Mass: 62.068 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C₂H₆O₂
#4: Chemical	ChemComp-IMD / IMIDAZOLE / Imidazole Mass: 69.085 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₃H₅N₂
#5: Water	ChemComp-HOH / water / Water Mass: 18.015 Da / Num. of mol.: 1256 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.35 Å³/Da / Density % sol: 47.8 %
Crystal grow	Temperature: 290 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: Molecular Dimensions Morpheus screen G2: 10% w/v PEG 8000, 20% v/v ethylene glycol: 20mM of each sodium formate, ammonium acetate, trisodium citrate, sodium potassium L-tartrate, sodium ...Details: Molecular Dimensions Morpheus screen G2: 10% w/v PEG 8000, 20% v/v ethylene glycol: 20mM of each sodium formate, ammonium acetate, trisodium citrate, sodium potassium L-tartrate, sodium oxamate: 100mM MES/imidazole pH 6.5: BuceA.00010.g.B1.PW37236 at 20.78mg/ml + 4mM NADP: cryo: direct: tray 285571g2, puck yrq2-3

Crystal

Density Matthews: 2.35 Å³/Da / Density % sol: 47.8 %

Crystal grow

Temperature: 290 K / Method: vapor diffusion, sitting drop / pH: 6.5
Details: Molecular Dimensions Morpheus screen G2: 10% w/v PEG 8000, 20% v/v ethylene glycol: 20mM of each sodium formate, ammonium acetate, trisodium citrate, sodium potassium L-tartrate, sodium ...

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Data collection

Diffraction

Mean temperature: 100 K

Diffraction source

Source:

ROTATING ANODE / Type: RIGAKU FR-E+ SUPERBRIGHT / Wavelength: 1.5418 Å

Detector

Type: RIGAKU SATURN 944+ / Detector: CCD / Date: Nov 24, 2016

Radiation

Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray

Radiation wavelength

Wavelength: 1.5418 Å / Relative weight: 1

Reflection

Resolution: 1.55→43.945 Å / Num. obs: 137073 / % possible obs: 98 % / Observed criterion σ(I): -3 / Redundancy: 14.301 % / B_iso Wilson estimate: 10.88 Å² / CC_1/2: 1 / R_merge(I) obs: 0.047 / Net I/σ(I): 38.65

Reflection shell

Diffraction-ID: 1

Resolution (Å)	Redundancy (%)	R_merge(I) obs	Mean I/σ(I) obs	Num. unique all	CC_1/2	% possible all
1.55-1.59	8.309	0.39	6.01	12623	0.911	90.4
1.59-1.63	10.356	0.32	7.64		0.951	94
1.63-1.68	11.294	0.266	8.98		0.97	95.5
1.68-1.73	11.634	0.222	10.77		0.981	96.7
1.73-1.79	12.032	0.191	12.53		0.988	98
1.79-1.85	12.445	0.157	15.03		0.992	98.8
1.85-1.92	12.876	0.121	19.08		0.996	99.6
1.92-2	13.291	0.102	24.04		0.997	99.9
2-2.09	13.878	0.081	30.06		0.998	99.9
2.09-2.19	14.336	0.064	38.68		0.999	100
2.19-2.31	13.158	0.067	42.5		0.999	99.8
2.31-2.45	15.025	0.053	49.93		0.999	99.8
2.45-2.62	14.13	0.054	54.37		0.999	99.8
2.62-2.83	16.252	0.042	63.99		1	100
2.83-3.1	17.532	0.035	75.25		1	99.9
3.1-3.47	18.212	0.035	86.75		1	100
3.47-4	23.109	0.027	113.33		1	99.9
4-4.9	29.039	0.025	137.29		1	99.8
4.9-6.93	29.48	0.026	127.62		1	100
6.93-43.945	27.922	0.022	139.72		0.999	99.3

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Phasing

Phasing	Method: molecular replacement

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Processing

Software

Name	Version	Classification
XDS		data reduction
XSCALE		data scaling
MOLREP		phasing
PHENIX		model building
Coot		model building
PHENIX	(dev_2597)	refinement
PDB_EXTRACT		data extraction

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: PDB entry 3v2g via Balbes

3v2g

Resolution: 1.55→43.945 Å / SU ML: 0.12 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 15.22

	R_factor	Num. reflection	% reflection
R_free	0.1632	2019	1.47 %
R_work	0.1369	-	-
obs	0.1373	137043	98.04 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å

Displacement parameters

B_iso max: 61.99 Å² / B_iso mean: 15.5888 Å² / B_iso min: 4.38 Å²

Refinement step

Cycle: final / Resolution: 1.55→43.945 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	6919	0	244	1314	8477
B_iso mean	-	-	12.98	28.64	-
Num. residues	-	-	-	-	981

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.008	7512
X-RAY DIFFRACTION	f_angle_d	1.017	10261
X-RAY DIFFRACTION	f_chiral_restr	0.064	1206
X-RAY DIFFRACTION	f_plane_restr	0.006	1454
X-RAY DIFFRACTION	f_dihedral_angle_d	12.951	4622

LS refinement shell

Refine-ID: X-RAY DIFFRACTION / R_factor R_free error: 0 / Total num. of bins used: 14

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Num. reflection all	% reflection obs (%)
1.55-1.5888	0.2414	127	0.1864	8807	8934	90
1.5888-1.6317	0.1922	152	0.156	9173	9325	94
1.6317-1.6797	0.1714	136	0.1479	9375	9511	95
1.6797-1.734	0.1914	146	0.1433	9475	9621	97
1.734-1.7959	0.1618	123	0.1389	9642	9765	98
1.7959-1.8678	0.1537	137	0.1385	9658	9795	99
1.8678-1.9529	0.1819	148	0.1392	9817	9965	100
1.9529-2.0558	0.1774	158	0.138	9779	9937	100
2.0558-2.1846	0.136	138	0.129	9837	9975	100
2.1846-2.3533	0.1339	145	0.1306	9828	9973	100
2.3533-2.5901	0.1823	158	0.1362	9835	9993	100
2.5901-2.9648	0.1692	155	0.1372	9848	10003	100
2.9648-3.735	0.1587	148	0.1304	9894	10042	100
3.735-43.9629	0.1449	148	0.1319	10056	10204	100

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.4319	-0.1943	0.2585	4.9011	-0.9326	3.0259	-0.018	0.4541	0.0571	-0.276	-0.032	0.0549	-0.1293	-0.0724	0.0225	0.0658	-0.0037	0.0277	0.1439	0.0015	0.0549	49.1409	3.8121	1.3809
2	2.579	0.2712	0.1985	1.5181	0.2522	2.3267	-0.0784	0.1835	-0.0818	-0.0544	0.051	-0.0596	0.021	0.0377	0.0125	0.0603	0.002	0.0338	0.0732	-0.0206	0.0691	51.6608	-1.7636	3.5201
3	1.2221	0.0905	0.6855	1.4895	1.3364	5.2645	-0.0082	0.2601	-0.1336	-0.1994	0.0577	-0.1345	-0.0824	0.3262	-0.0347	0.0814	0.0077	0.0393	0.1499	-0.0175	0.1335	58.5004	-1.0938	2.5199
4	4.058	-1.8917	3.1243	3.8909	-4.277	7.2854	-0.093	0.0688	0.2188	0.1254	-0.1541	-0.3709	-0.4057	0.51	0.145	0.0677	-0.023	0.0103	0.0971	-0.0005	0.121	59.425	10.0529	9.9047
5	0.9127	-0.5197	0.1998	2.0486	-2.3584	4.6905	-0.0454	-0.1128	-0.1192	-0.0344	-0.0088	-0.0972	0.2563	0.2156	0.0463	0.0616	0.0204	0.0022	0.0682	0	0.1017	51.8892	-3.7806	22.7772
6	0.747	-0.0784	-0.0262	1.0497	-0.6818	1.2688	-0.005	-0.0093	0.0527	-0.0309	0.0187	0.0342	0.0059	0.0099	-0.009	0.0422	-0.0017	-0.0027	0.0419	-0.0131	0.0472	43.645	4.4881	18.666
7	1.3165	-0.6958	-0.0807	3.8853	0.5035	0.4876	-0.0543	-0.0092	-0.2508	0.0709	0.0278	-0.157	0.1401	0.0997	0.0024	0.0902	0.0139	0.0162	0.061	-0.0004	0.1141	43.3673	-8.5162	13.6982
8	1.0649	0.7657	-0.7622	3.6574	-1.8156	2.0088	-0.1024	0.0247	-0.2159	-0.1069	0.0706	-0.1282	0.2358	0.0409	0.0354	0.0808	0.002	-0.0027	0.0635	-0.0194	0.0931	37.5066	-13.0226	10.4636
9	1.4651	0.0143	0.1733	0.2305	0.2197	1.1266	-0.0029	0.0657	0.0888	-0.0893	0.0095	-0.0123	-0.027	0.052	0.0008	0.0442	-0.0054	0.0032	0.0529	-0.0139	0.0593	34.1489	1.1783	10.4504
10	2.3097	0.9235	-0.0221	5.7917	-0.809	1.651	0.0366	-0.406	-0.1215	0.213	0.0597	0.2146	0.125	-0.215	-0.0855	0.1142	-0.0128	-0.0205	0.2259	0.0203	0.0634	39.2337	0.4515	53.0192
11	2.1845	0.0309	0.1531	0.9853	-0.1887	1.7697	-0.0741	-0.2623	0.0617	0.016	0.0838	0.0171	-0.0097	-0.0962	-0.0177	0.0985	0.0244	-0.0062	0.1483	-0.0301	0.0476	40.8852	6.4112	51.9754
12	0.7883	-0.449	-0.3872	0.7862	0.9347	2.2152	-0.0757	-0.2299	0.0527	0.1252	0.1123	-0.0305	0.0733	0.1282	-0.0346	0.1108	0.0182	-0.0113	0.164	-0.0334	0.0909	46.4989	6.9984	55.7295
13	6.1182	2.1018	-3.9782	1.9256	-1.519	2.849	-0.1023	-0.3846	-0.1339	0.0549	0.0498	-0.1608	0.3386	0.3394	0.0767	0.094	0.033	-0.0284	0.1729	-0.02	0.0972	52.8763	-3.775	49.5177
14	1.0017	0.5662	-0.4667	1.9686	-1.5063	3.2242	-0.0131	-0.0053	0.0398	-0.0003	0.0335	-0.065	-0.1201	0.1195	-0.0053	0.0588	-0.0093	-0.0214	0.0861	-0.0286	0.071	48.5078	4.6508	36.241
15	0.9695	-0.804	0.853	2.5514	-2.6417	3.8657	0.0336	-0.0749	-0.037	-0.0252	0.1256	0.1475	-0.0002	-0.1441	-0.1649	0.0529	0.0021	-0.0059	0.0647	-0.0114	0.0544	36.8008	2.7165	35.5873
16	0.9716	-0.0412	0.1014	0.1952	0.2568	0.7491	-0.0241	-0.0859	0.0463	0.0412	0.016	-0.0071	-0.0532	0.0246	0.0089	0.0684	0.0066	-0.0104	0.0781	-0.005	0.0542	38.283	3.3031	36.0496
17	0.6018	-0.4162	0.613	3.1412	-2.2011	2.6903	-0.0742	-0.0821	0.1168	0.068	0.0214	-0.0536	-0.2256	-0.0122	0.0409	0.1032	0.0221	-0.0061	0.1193	-0.0435	0.0916	28.469	14.1537	39.5778
18	0.6574	-0.5327	0.4229	3.8476	-1.1578	0.8272	0.0048	-0.1538	-0.0155	0.1277	0.0258	-0.0421	0.0033	-0.047	-0.0392	0.0619	0.0102	-0.0053	0.1	-0.0014	0.0498	28.1985	0.3188	38.6809
19	2.7627	-0.7633	-0.2801	4.9761	0.7019	2.1706	-0.0196	0.337	-0.1325	-0.1884	-0.0677	0.0019	0.0496	0.0944	0.0395	0.0594	-0.0068	-0.0171	0.0954	0.0034	0.0164	19.4902	-4.1425	-4.2471
20	3.1355	-0.0111	-0.1651	1.5667	0.1515	1.7532	-0.0426	0.1186	0.0265	-0.0489	0.0015	-0.0241	-0.0339	-0.0152	0.0281	0.0665	-0.0065	-0.0118	0.0519	0.0143	0.0229	16.6352	1.3852	-3.7296
21	3.4066	-3.3446	-3.9042	5.213	2.0717	6.4663	0.0604	0.3489	0.1499	-0.3174	-0.0703	-0.0984	-0.1929	-0.0764	-0.0344	0.1372	-0.0259	-0.0447	0.139	0.0448	0.0958	13.3399	3.8863	-11.1925
22	2.028	0.0449	-1.99	1.2243	-0.1414	4.5714	0.0139	0.1007	0.0632	-0.1531	0.0351	-0.0065	-0.01	0.0301	-0.0309	0.0655	-0.0083	-0.024	0.0764	-0.0041	0.0495	9.6665	-2.8723	-4.3187
23	4.4829	-2.893	-4.964	2.6616	3.1171	5.5008	-0.1357	0.1589	-0.1915	0.0081	-0.0114	0.1618	0.2851	-0.2451	0.2038	0.0759	-0.0063	-0.0127	0.0958	-0.0049	0.0975	6.8592	-10.5967	-0.0454
24	1.3572	-0.7196	-0.6136	1.6765	1.1041	2.6753	-0.0149	-0.0477	0.0864	0.0365	0.0466	0.0144	-0.107	-0.1322	-0.0238	0.038	0.0059	-0.0164	0.0579	0.0127	0.0574	9.4399	0.3513	12.4206
25	0.9044	0.2027	0.1977	1.4268	1.2297	1.818	0.0123	-0.012	0.0384	0.0025	0.1071	-0.1456	-0.0734	0.0976	-0.1003	0.0591	0.0051	-0.0102	0.0394	0.0067	0.0608	20.919	0.2942	13.106
26	1.1073	-1.0716	-0.3127	2.439	0.8462	0.798	0.0123	-0.0207	-0.0484	0.0225	0.0118	-0.043	0.0327	-0.0307	-0.0189	0.0558	-0.0086	-0.0064	0.0547	0.0116	0.0425	18.207	-4.4242	17.0474
27	1.4837	-0.6852	-0.2541	3.891	-0.2795	1.7227	-0.0015	0.0362	-0.134	-0.1851	0.0426	0.1842	0.1618	-0.067	0.0122	0.063	-0.01	-0.0149	0.032	0.0012	0.0639	23.6673	-8.7266	9.8113
28	3.2615	0.2999	1.0914	7.4366	-0.8885	0.4991	-0.2089	-0.1741	0.7118	0.1104	0.0158	0.4089	-0.2296	-0.2862	0.1281	0.1574	0.0165	-0.05	0.0961	-0.0138	0.2026	18.114	17.0817	7.6681
29	0.7823	-0.0193	0.5038	3.9427	2.0645	2.3616	-0.0874	0.036	0.1518	-0.1325	0.0193	0.0932	-0.241	-0.0242	0.0576	0.082	-0.0186	-0.0021	0.0717	0.0203	0.0922	27.1946	12.7969	7.7972
30	0.8113	0.267	0.2896	2.5268	0.8594	0.883	-0.0102	0.0769	-0.0319	-0.0977	0.0449	-0.0001	0.0062	0.0386	-0.0451	0.0636	-0.0114	-0.0031	0.0582	0.0001	0.0636	30.14	-0.7637	9.9859
31	2.3691	0.3278	-0.0901	5.8383	0.9098	2.0588	0.1031	-0.3093	0.1978	0.329	-0.0337	-0.0461	-0.107	-0.0627	-0.0687	0.0973	0.0091	0.0315	0.1923	-0.0092	0.0707	8.1094	1.006	45.938
32	0.9917	0.2983	-0.2117	1.2539	-0.2232	1.124	-0.0136	-0.3296	0.0042	0.1332	0.0225	0.1375	-0.012	-0.0598	-0.0171	0.0991	0.0183	0.029	0.1909	0.0313	0.0907	4.9918	-6.4382	46.9782
33	1.1531	0.4142	-0.0983	1.9779	0.9783	4.7949	-0.0142	-0.1975	0.1994	0.1017	0.0487	0.2965	-0.1408	-0.2635	0.0086	0.0777	0.0393	0.0442	0.2186	0.0214	0.1658	-1.5434	1.9602	39.9832
34	1.0796	-0.2858	-0.3149	1.1858	0.6097	1.6089	-0.0163	-0.0514	-0.0181	0.0555	0.0421	0.0917	0.0539	-0.1363	-0.0104	0.0579	-0.0083	0.0097	0.085	0.0342	0.0688	10.4241	-3.3383	28.8919
35	0.7539	0.0301	-0.063	0.1392	-0.1847	0.888	-0.0331	-0.0966	-0.0749	0.0261	0.0223	0.0474	0.0378	-0.0841	0.0079	0.048	0.0069	0.0101	0.0882	0.0151	0.0636	15.9881	-2.4307	31.52
36	1.5433	-1.6174	-1.1376	3.7325	1.9014	1.9326	-0.1166	-0.1498	-0.1793	0.1474	0.0722	0.1459	0.1931	-0.0212	0.0585	0.0734	-0.0033	-0.0065	0.1211	0.0441	0.0768	23.418	-12.9327	39.1957
37	1.8855	-0.443	0.0928	1.0533	-0.6939	0.6155	0.0036	-0.1576	0.0599	0.0508	-0.0244	0.0765	-0.0966	-0.0549	0.0129	0.0593	0.0023	0.0013	0.1051	0.0045	0.0432	24.0769	1.3917	38.1462

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 1 through 16 )	A	1 - 16
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 17 through 40 )	A	17 - 40
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 41 through 67 )	A	41 - 67
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 68 through 82 )	A	68 - 82
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 83 through 116 )	A	83 - 116
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 117 through 176 )	A	117 - 176
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 177 through 199 )	A	177 - 199
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 200 through 225 )	A	200 - 225
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 226 through 246 )	A	226 - 246
10	X-RAY DIFFRACTION	10	chain 'B' and (resid 2 through 16 )	B	2 - 16
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 17 through 40 )	B	17 - 40
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 41 through 67 )	B	41 - 67
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 68 through 82 )	B	68 - 82
14	X-RAY DIFFRACTION	14	chain 'B' and (resid 83 through 128 )	B	83 - 128
15	X-RAY DIFFRACTION	15	chain 'B' and (resid 129 through 152 )	B	129 - 152
16	X-RAY DIFFRACTION	16	chain 'B' and (resid 153 through 199 )	B	153 - 199
17	X-RAY DIFFRACTION	17	chain 'B' and (resid 200 through 225 )	B	200 - 225
18	X-RAY DIFFRACTION	18	chain 'B' and (resid 226 through 246 )	B	226 - 246
19	X-RAY DIFFRACTION	19	chain 'C' and (resid 2 through 16 )	C	2 - 16
20	X-RAY DIFFRACTION	20	chain 'C' and (resid 17 through 40 )	C	17 - 40
21	X-RAY DIFFRACTION	21	chain 'C' and (resid 41 through 54 )	C	41 - 54
22	X-RAY DIFFRACTION	22	chain 'C' and (resid 55 through 67 )	C	55 - 67
23	X-RAY DIFFRACTION	23	chain 'C' and (resid 68 through 82 )	C	68 - 82
24	X-RAY DIFFRACTION	24	chain 'C' and (resid 83 through 128 )	C	83 - 128
25	X-RAY DIFFRACTION	25	chain 'C' and (resid 129 through 152 )	C	129 - 152
26	X-RAY DIFFRACTION	26	chain 'C' and (resid 153 through 173 )	C	153 - 173
27	X-RAY DIFFRACTION	27	chain 'C' and (resid 174 through 185 )	C	174 - 185
28	X-RAY DIFFRACTION	28	chain 'C' and (resid 186 through 199 )	C	186 - 199
29	X-RAY DIFFRACTION	29	chain 'C' and (resid 200 through 225 )	C	200 - 225
30	X-RAY DIFFRACTION	30	chain 'C' and (resid 226 through 246 )	C	226 - 246
31	X-RAY DIFFRACTION	31	chain 'D' and (resid 2 through 16 )	D	2 - 16
32	X-RAY DIFFRACTION	32	chain 'D' and (resid 17 through 54 )	D	17 - 54
33	X-RAY DIFFRACTION	33	chain 'D' and (resid 55 through 82 )	D	55 - 82
34	X-RAY DIFFRACTION	34	chain 'D' and (resid 83 through 152 )	D	83 - 152
35	X-RAY DIFFRACTION	35	chain 'D' and (resid 153 through 199 )	D	153 - 199
36	X-RAY DIFFRACTION	36	chain 'D' and (resid 200 through 225 )	D	200 - 225
37	X-RAY DIFFRACTION	37	chain 'D' and (resid 226 through 246 )	D	226 - 246

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