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- PDB-5tu8: Crystal structure of Staphylococcus epidermidis Aap G58-spacer-G5... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5tu8 | |||||||||||||||||||||||||||||||
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Title | Crystal structure of Staphylococcus epidermidis Aap G58-spacer-G513* (variant G5-spacer-variant G5) | |||||||||||||||||||||||||||||||
![]() | Aap G58-spacer-G513*![]() ![]() Function / homology | ![]() Biological species | ![]() ![]() ![]() Method | ![]() ![]() ![]() ![]() Shelton, C.L. / Conrady, D.G. / Herr, A.B. | Funding support | | ![]()
![]() ![]() Title: Functional consequences of B-repeat sequence variation in the staphylococcal biofilm protein Aap: deciphering the assembly code. Authors: Shelton, C.L. / Conrady, D.G. / Herr, A.B. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 71 KB | Display | ![]() |
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PDB format | ![]() | 50.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 5tu7C ![]() 5tu9C ![]() 4fupS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 22582.828 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.42 Å3/Da / Density % sol: 49.09 % |
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Crystal grow![]() | Temperature: 293.15 K / Method: vapor diffusion, hanging drop Details: 100 mM sodium acetate pH 5 175 mM calcium chloride 22% PEG 6000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Mar 28, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.33→92.25 Å / Biso Wilson estimate: 48.9 Å2 / Rmerge(I) obs: 0.15 |
Reflection shell | Resolution: 2.33→2.413 Å / Redundancy: 4.6 % / Rmerge(I) obs: 0.72 / Mean I/σ(I) obs: 2.52 / % possible all: 89 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 4FUP Resolution: 2.33→92.25 Å / Cor.coef. Fo:Fc: 0.9146 / Cor.coef. Fo:Fc free: 0.8903 / SU R Cruickshank DPI: 0.23 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.257 / SU Rfree Blow DPI: 0.206 / SU Rfree Cruickshank DPI: 0.197
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Displacement parameters | Biso mean: 50.38 Å2
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Refine analyze | Luzzati coordinate error obs: 0.281 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: 1 / Resolution: 2.33→92.25 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.33→2.46 Å / Total num. of bins used: 10
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