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- PDB-5suh: The structure of double ringed trimeric shell protein MSM0271 fro... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5suh | ||||||
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Title | The structure of double ringed trimeric shell protein MSM0271 from the RMM microcompartment | ||||||
![]() | MSM0271 protein | ||||||
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Function / homology | BMC (bacterial microcompartment) domain / Alpha-Beta Plaits / 2-Layer Sandwich / Alpha Beta / Uncharacterized protein![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Mallette, E. / Kimber, M.S. | ||||||
![]() | ![]() Title: A Complete Structural Inventory of the Mycobacterial Microcompartment Shell Proteins Constrains Models of Global Architecture and Transport. Authors: Mallette, E. / Kimber, M.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 245.7 KB | Display | ![]() |
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PDB format | ![]() | 200.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26019.549 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 700084 / mc(2)155 / Gene: MSMEG_0271, MSMEI_0264 / Plasmid: pET28a / Production host: ![]() ![]() ![]() #2: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.72 Å3/Da / Density % sol: 54.71 % / Description: hexagonal prisms |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 10 mg/mL protein, 0.8 M Na/K tartrate, 0.1 M Tris, 0.5% (w/v) PEG 2000MME |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX-300 / Detector: CCD / Date: Jul 10, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.7→47.2 Å / Num. obs: 24387 / % possible obs: 99.9 % / Redundancy: 9.6 % / Biso Wilson estimate: 69.4 Å2 / CC1/2: 0.997 / Rsym value: 0.133 / Net I/σ(I): 19.2 |
Reflection shell | Resolution: 2.7→2.77 Å / Redundancy: 11.1 % / Mean I/σ(I) obs: 2.1 / CC1/2: 0.752 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: MSM0275 Resolution: 2.7→47.139 Å / SU ML: 0.37 / Cross valid method: THROUGHOUT / σ(F): 1.37 / Phase error: 22.9
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→47.139 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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