PDB-5qu6: Crystal Structure of swapped human Nck SH3.1 domain, 1.8A, triclinic

Basic information

• Entry: Database: PDB / ID: 5qu6
• Title: Crystal Structure of swapped human Nck SH3.1 domain, 1.8A, triclinic
• Components: Cytoplasmic protein NCK1Cytoplasm
• Keywords: SIGNALING PROTEIN / SH3 DOMAIN / ADAPTOR / PEPTIDE BINDING / DOMAIN SWAP

Function / homology

Function:

positive regulation of cap-dependent translational initiation / negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation / eukaryotic initiation factor eIF2 binding / positive regulation of peptidyl-serine dephosphorylation / protein phosphatase type 1 complex / positive regulation of translation in response to endoplasmic reticulum stress / positive regulation of cap-independent translational initiation / positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway / cytoskeletal anchor activity / substrate-dependent cell migration, cell extension / vesicle membrane / signal complex assembly / Activation of RAC1 / Nephrin family interactions / DCC mediated attractive signaling / lamellipodium assembly / RHOV GTPase cycle / positive regulation of actin filament polymerization / negative regulation of PERK-mediated unfolded protein response / protein kinase inhibitor activity / antiviral innate immune response / RHOU GTPase cycle / Generation of second messenger molecules / RHO GTPases Activate WASPs and WAVEs / ephrin receptor signaling pathway / signaling adaptor activity / negative regulation of peptidyl-serine phosphorylation / positive regulation of T cell proliferation / regulation of cell migration / T cell activation / negative regulation of insulin receptor signaling pathway / response to endoplasmic reticulum stress / ephrin receptor binding / Downstream signal transduction / Antigen activates B Cell Receptor (BCR) leading to generation of second messengers / molecular condensate scaffold activity / actin filament organization / FCGR3A-mediated phagocytosis / PKR-mediated signaling / receptor tyrosine kinase binding / Regulation of actin dynamics for phagocytic cup formation / VEGFA-VEGFR2 Pathway / positive regulation of neuron projection development / cell-cell junction / signaling receptor complex adaptor activity / cell migration / protein-macromolecule adaptor activity / Potential therapeutics for SARS / ribosome / cadherin binding / protein domain specific binding / signaling receptor binding / negative regulation of transcription by RNA polymerase II / endoplasmic reticulum / signal transduction / positive regulation of transcription by RNA polymerase II / nucleus / plasma membrane / cytosol / cytoplasm

Domain/homology:

Nck1, SH3 domain 1 / Nck1, SH3 domain 2 / Nck1, SH3 domain 3 / Nck1, SH2 domain / Cytoplasmic protein NCK / SH3 domain / SH2 domain / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / Src homology 3 domains / SH2 domain superfamily / SH3-like domain superfamily / Src homology 3 (SH3) domain profile. / SH3 domain

Component:

SH2/SH3 adapter protein NCK1

• Biological species: Homo sapiens (human)
• Method: X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.816 Å
• Authors: Rudolph, M.G.
• Citation: Journal: J.Biol.Chem. / Year: 2020; Title: Small molecule AX-024 reduces T cell proliferation independently of CD3ε/Nck1 interaction, which is governed by a domain swap in the Nck1-SH3.1 domain.; Authors: Richter, K. / Rufer, A.C. / Muller, M. / Burger, D. / Casagrande, F. / Grossenbacher, T. / Huber, S. / Hug, M.N. / Koldewey, P. / D'Osualdo, A. / Schlatter, D. / Stoll, T. / Rudolph, M.G.

History

Deposition	Dec 13, 2019	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Feb 12, 2020	Provider: repository / Type: Initial release
Revision 1.1	May 12, 2021	Group: Structure summary / Category: pdbx_deposit_group Item: _pdbx_deposit_group.group_description / _pdbx_deposit_group.group_type
Revision 1.2	Jun 30, 2021	Group: Database references / Category: citation / citation_author Item: _citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.3	Mar 6, 2024	Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2 Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Revision 1.4	Apr 3, 2024	Group: Database references / Refinement description / Category: citation / pdbx_initial_refinement_model / Item: _citation.title

Assembly

Deposited unit

A: Cytoplasmic protein NCK1

B: Cytoplasmic protein NCK1

C: Cytoplasmic protein NCK1

D: Cytoplasmic protein NCK1

E: Cytoplasmic protein NCK1

F: Cytoplasmic protein NCK1

G: Cytoplasmic protein NCK1

H: Cytoplasmic protein NCK1

I: Cytoplasmic protein NCK1

J: Cytoplasmic protein NCK1

K: Cytoplasmic protein NCK1

L: Cytoplasmic protein NCK1

M: Cytoplasmic protein NCK1

N: Cytoplasmic protein NCK1

O: Cytoplasmic protein NCK1

P: Cytoplasmic protein NCK1

Q: Cytoplasmic protein NCK1

R: Cytoplasmic protein NCK1

S: Cytoplasmic protein NCK1

T: Cytoplasmic protein NCK1

U: Cytoplasmic protein NCK1

V: Cytoplasmic protein NCK1

W: Cytoplasmic protein NCK1

X: Cytoplasmic protein NCK1

Y: Cytoplasmic protein NCK1

Z: Cytoplasmic protein NCK1

1: Cytoplasmic protein NCK1

2: Cytoplasmic protein NCK1

Summary:

• 288 kDa, 28 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	288,383	28
Polymers	288,383	28
Non-polymers	0	0
Water	3,837	213

1

A: Cytoplasmic protein NCK1

C: Cytoplasmic protein NCK1

Summary:

• defined by author&software
• Evidence: gel filtration, elutes as a monomer
• 20.6 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	20,599	2
Polymers	20,599	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4070 Å2
ΔGint	-21 kcal/mol
Surface area	7030 Å2
Method	PISA

2

B: Cytoplasmic protein NCK1

D: Cytoplasmic protein NCK1

Summary:

• defined by author&software
• 20.6 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	20,599	2
Polymers	20,599	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4100 Å2
ΔGint	-25 kcal/mol
Surface area	6910 Å2
Method	PISA

3

E: Cytoplasmic protein NCK1

F: Cytoplasmic protein NCK1

Summary:

• defined by author&software
• 20.6 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	20,599	2
Polymers	20,599	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4200 Å2
ΔGint	-25 kcal/mol
Surface area	7140 Å2
Method	PISA

4

G: Cytoplasmic protein NCK1

H: Cytoplasmic protein NCK1

Summary:

• defined by author&software
• 20.6 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	20,599	2
Polymers	20,599	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4170 Å2
ΔGint	-23 kcal/mol
Surface area	7190 Å2
Method	PISA

5

I: Cytoplasmic protein NCK1

J: Cytoplasmic protein NCK1

Summary:

• defined by author&software
• 20.6 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	20,599	2
Polymers	20,599	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4030 Å2
ΔGint	-27 kcal/mol
Surface area	7100 Å2
Method	PISA

6

K: Cytoplasmic protein NCK1

L: Cytoplasmic protein NCK1

Summary:

• defined by author&software
• 20.6 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	20,599	2
Polymers	20,599	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4110 Å2
ΔGint	-22 kcal/mol
Surface area	7310 Å2
Method	PISA

7

M: Cytoplasmic protein NCK1

N: Cytoplasmic protein NCK1

Summary:

• defined by author&software
• 20.6 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	20,599	2
Polymers	20,599	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4040 Å2
ΔGint	-23 kcal/mol
Surface area	6760 Å2
Method	PISA

8

O: Cytoplasmic protein NCK1

P: Cytoplasmic protein NCK1

Summary:

• defined by author&software
• 20.6 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	20,599	2
Polymers	20,599	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4090 Å2
ΔGint	-23 kcal/mol
Surface area	7070 Å2
Method	PISA

9

Q: Cytoplasmic protein NCK1

R: Cytoplasmic protein NCK1

Summary:

• defined by author&software
• 20.6 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	20,599	2
Polymers	20,599	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4190 Å2
ΔGint	-21 kcal/mol
Surface area	6750 Å2
Method	PISA

10

S: Cytoplasmic protein NCK1

T: Cytoplasmic protein NCK1

Summary:

• defined by author&software
• 20.6 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	20,599	2
Polymers	20,599	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4200 Å2
ΔGint	-25 kcal/mol
Surface area	6930 Å2
Method	PISA

11

U: Cytoplasmic protein NCK1

V: Cytoplasmic protein NCK1

Summary:

• defined by author&software
• 20.6 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	20,599	2
Polymers	20,599	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	4040 Å2
ΔGint	-20 kcal/mol
Surface area	6740 Å2
Method	PISA

12

W: Cytoplasmic protein NCK1

X: Cytoplasmic protein NCK1

Summary:

• defined by author&software
• 20.6 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	20,599	2
Polymers	20,599	2
Non-polymers	0	0
Water	36	2

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	3980 Å2
ΔGint	-24 kcal/mol
Surface area	6900 Å2
Method	PISA

13

Y: Cytoplasmic protein NCK1

Z: Cytoplasmic protein NCK1

Summary:

• defined by author&software
• 20.6 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	20,599	2
Polymers	20,599	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	3690 Å2
ΔGint	-24 kcal/mol
Surface area	6880 Å2
Method	PISA

14

1: Cytoplasmic protein NCK1

2: Cytoplasmic protein NCK1

Summary:

• defined by author&software
• 20.6 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	20,599	2
Polymers	20,599	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	3610 Å2
ΔGint	-22 kcal/mol
Surface area	6330 Å2
Method	PISA

Unit cell

Length a, b, c (Å)	49.083, 92.053, 114.748
Angle α, β, γ (deg.)	103.020, 91.630, 88.570
Int Tables number	1
Space group name H-M	P1

Components

#1: Protein

A B C D E F G H I J K L M N O P Q R S ...
Cytoplasmic protein NCK1 / Cytoplasm / NCK adaptor protein 1 / Nck-1 / SH2/SH3 adaptor protein NCK-alpha

Details:

Mass: 10299.410 Da / Num. of mol.: 28 / Fragment: SH3 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: NCK1, NCK / Plasmid: PET28a(+) / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P16333

#2: Water

ChemComp-HOH / water / Water

Details:

Mass: 18.015 Da / Num. of mol.: 213 / Source method: isolated from a natural source / Formula: H₂O

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 1.75 ų/Da / Density % sol: 29.74 %
• Crystal grow: Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.5 ; Details: 0.1 M Bis-Tris/HCl pH 6.5, 2% tacsimate pH 6.0, 20% PEG 3350

Data collection

• Diffraction: Mean temperature: 100 K
• Diffraction source: Source: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 1 Å
• Detector: Type: PSI PILATUS 6M / Detector: PIXEL / Date: Apr 10, 2018
• Radiation: Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 1 Å / Relative weight: 1
• Reflection: Resolution: 1.81→53.083 Å / Num. obs: 170464 / % possible obs: 95.6 % / Redundancy: 1.98 % / B_iso Wilson estimate: 32.62 Ų / CC_1/2: 0.999 / R_merge(I) obs: 0.052 / R_rim(I) all: 0.07 / Χ²: 0.856 / Net I/σ(I): 8.43 / Num. measured all: 337282 / Scaling rejects: 82

Reflection shell

Diffraction-ID: 1

Resolution (Å)	Redundancy (%)	Rmerge(I) obs	Mean I/σ(I) obs	Num. measured obs	Num. possible	Num. unique obs	CC1/2	Rrim(I) all	% possible all
1.81-1.86	1.968	2.95	0.27	24393	13114	12397	0.122	3.998	94.5
1.86-1.91	1.866	1.901	0.4	22749	12958	12190	0.197	2.578	94.1
1.91-1.96	1.93	1.458	0.58	22855	12511	11840	0.263	1.979	94.6
1.96-2.02	2.059	1.032	0.88	23968	12114	11642	0.352	1.398	96.1
2.02-2.09	2.054	0.78	1.13	23040	11755	11218	0.459	1.057	95.4
2.09-2.16	2.039	0.558	1.6	22217	11406	10895	0.63	0.754	95.5
2.16-2.25	2.018	0.409	2.12	21356	11051	10584	0.748	0.554	95.8
2.25-2.34	1.994	0.272	3.09	20206	10590	10131	0.882	0.367	95.7
2.34-2.44	1.846	0.193	3.91	18034	10095	9769	0.928	0.261	96.8
2.44-2.56	1.965	0.171	4.68	18437	9696	9381	0.94	0.232	96.8
2.56-2.7	2.067	0.124	6.65	18403	9203	8903	0.968	0.167	96.7
2.7-2.86	2.049	0.089	9.22	17316	8730	8450	0.983	0.119	96.8
2.86-3.06	2.025	0.057	13.41	15867	8197	7835	0.993	0.077	95.6
3.06-3.3	1.951	0.042	17.85	14017	7628	7184	0.996	0.057	94.2
3.3-3.62	1.786	0.03	21.99	11821	6959	6619	0.998	0.04	95.1
3.62-4.05	2.009	0.024	28.7	12239	6371	6092	0.998	0.033	95.6
4.05-4.67	2.04	0.018	35.7	10912	5580	5348	0.999	0.025	95.8
4.67-5.72	1.97	0.017	35.25	8934	4737	4535	0.999	0.024	95.7
5.72-8.09	1.82	0.018	34.39	6393	3658	3512	0.999	0.024	96
8.09-53.083	2.127	0.013	43.29	4125	1987	1939	0.999	0.017	97.6

Processing

Software

Name	Version	Classification	NB
XSCALE		data scaling
PHENIX	1.12rc1_2801	refinement
PDB_EXTRACT	3.24	data extraction
XDS		data reduction
XSCALE		data scaling
PHASER		phasing

Refinement

Method to determine structure: MOLECULAR REPLACEMENT
Starting model: inhouse model

Resolution: 1.816→53.083 Å / SU ML: 0.3 / Cross valid method: THROUGHOUT / σ(F): 1.96 / Phase error: 39.29 / Stereochemistry target values: ML / Details: DIMERIC

	Rfactor	Num. reflection	% reflection	Selection details
Rfree	0.263	5422	4.94 %	random
Rwork	0.2082	104229	-	-
obs	0.211	109651	62.14 %	-

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
• Displacement parameters: B_iso max: 179.46 Ų / B_iso mean: 57.935 Ų / B_iso min: 12.03 Ų

Refinement step

Cycle: final / Resolution: 1.816→53.083 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	13132	0	0	213	13345
Biso mean	-	-	-	40.83	-
Num. residues	-	-	-	-	1546

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.008	13417
X-RAY DIFFRACTION	f_angle_d	0.907	18113
X-RAY DIFFRACTION	f_chiral_restr	0.062	1909
X-RAY DIFFRACTION	f_plane_restr	0.005	2286
X-RAY DIFFRACTION	f_dihedral_angle_d	16.334	8045

LS refinement shell

Refine-ID: X-RAY DIFFRACTION / R_factor R_free error: 0 / Total num. of bins used: 30

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	Num. reflection all	% reflection obs (%)
1.8161-1.8367	0.2802	2	0.3189	56	58	1
1.8367-1.8583	0.2489	5	0.2655	100	105	2
1.8583-1.881	0.2646	13	0.2943	186	199	3
1.881-1.9048	0.4167	17	0.2735	314	331	6
1.9048-1.9299	0.3629	24	0.2838	491	515	9
1.9299-1.9563	0.3854	31	0.2963	694	725	12
1.9563-1.9843	0.3884	43	0.2851	861	904	16
1.9843-2.0139	0.3358	63	0.2953	1177	1240	21
2.0139-2.0453	0.3436	79	0.2911	1529	1608	27
2.0453-2.0789	0.3373	116	0.2866	1844	1960	34
2.0789-2.1147	0.3253	117	0.284	2276	2393	41
2.1147-2.1532	0.3246	155	0.2859	2786	2941	50
2.1532-2.1946	0.3559	191	0.272	3311	3502	59
2.1946-2.2394	0.3295	206	0.279	3748	3954	67
2.2394-2.2881	0.346	209	0.2775	4469	4678	80
2.2881-2.3413	0.3202	257	0.2659	5170	5427	92
2.3413-2.3999	0.2871	262	0.2641	5396	5658	97
2.3999-2.4647	0.3431	233	0.2772	5461	5694	96
2.4647-2.5373	0.3029	254	0.2829	5413	5667	97
2.5373-2.6192	0.2854	292	0.2826	5428	5720	97
2.6192-2.7128	0.3291	325	0.2768	5408	5733	97
2.7128-2.8214	0.3307	288	0.2773	5352	5640	97
2.8214-2.9498	0.3287	276	0.2667	5448	5724	96
2.9498-3.1053	0.2795	274	0.2419	5277	5551	95
3.1053-3.2998	0.2923	294	0.2238	5267	5561	94
3.2998-3.5545	0.2771	297	0.193	5279	5576	95
3.5545-3.9122	0.2753	289	0.1746	5332	5621	96
3.9122-4.478	0.1779	271	0.131	5395	5666	96
4.478-5.6408	0.1819	256	0.1353	5347	5603	96
5.6408-53.1062	0.2037	283	0.1746	5414	5697	97

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	4.9961	-1.1849	-0.8081	7.4976	6.8995	6.7283	0.1881	0.0044	-0.1873	-0.3292	0.2373	-0.375	-0.2016	0.0293	-0.3883	0.1232	-0.0838	-0.0564	0.3191	0.1179	0.2966	-19.6727	28.9686	-8.9467
2	2.7811	1.492	-1.858	3.3018	-3.8911	4.5911	0.6946	-1.0469	1.1202	1.323	-0.3245	0.2483	-1.4429	-0.0708	-0.5231	0.4079	-0.0621	0.1051	0.5389	-0.1742	0.4962	-19.2116	43.1138	-1.2406
3	4.9826	3.5514	1.2984	5.4682	1.1618	2.7793	0.1696	-0.3531	0.1152	0.262	0.0482	0.1447	0.0929	-0.5937	-0.1768	0.1215	-0.0531	-0.0599	0.3451	0.1212	0.2393	-22.1454	33.1506	-9.2113
4	7.5835	4.3849	6.7518	2.9415	4.0219	6.813	0.1459	2.5594	-1.7578	-0.6335	0.5569	-0.4252	1.375	1.495	-0.608	0.4314	0.0761	0.0456	0.7801	-0.2188	0.604	-8.4177	32.8047	-15.7915
5	6.4332	3.5337	0.6358	4.4322	0.8333	4.7683	0.0736	-0.2892	-0.121	0.0017	0.0448	-0.172	0.3371	-0.0378	-0.1242	0.088	-0.0205	0.0196	0.2346	-0.0291	0.4181	-2.3988	42.8519	-5.2769
6	7.5976	0.4371	-1.1815	5.0251	-0.1395	8.1503	0.2265	0.3299	-0.1317	-0.0416	-0.0291	0.0152	-0.2341	0.015	-0.122	0.0739	0.0408	-0.0029	0.0747	0.006	0.1542	22.3559	7.6138	-2.4485
7	7.5212	-0.7356	-4.9762	3.4848	0.6468	5.3426	0.2024	0.4803	0.713	-0.2001	0.0307	-0.5106	0.0264	0.0815	-0.206	0.1526	0.0751	0.0521	0.2885	0.1184	0.3135	6.5024	17.6029	-3.2516
8	8.223	1.472	-0.8664	5.2716	-1.2498	8.1752	-0.0444	-0.2296	0.1875	-0.3108	0.5004	-0.1891	-0.0139	0.1722	-0.3859	0.1507	-0.0681	0.0329	0.1687	-0.0705	0.4287	0.4633	44.7285	-7.2362
9	0.2382	0.4694	0.7977	1.0548	1.6173	2.6829	-0.3207	0.283	0.9614	-0.3578	0.3098	-0.2138	-0.1532	1.1549	0.0989	0.3256	-0.0768	-0.0406	0.3913	0.0264	0.5599	-7.3963	35.8142	1.481
10	8.7865	1.5292	4.2894	3.5941	0.9796	6.703	-0.0087	0.2773	0.0312	-0.0876	-0.0391	-0.3298	0.2576	0.1966	0.0315	0.1474	-0.008	-0.0358	0.1585	0.0798	0.1975	-17.2342	34.2886	-10.391
11	6.2309	-2.4504	-0.1994	8.5086	2.104	5.2917	0.1465	0.7733	0.7017	-0.1796	-0.267	-0.5295	-0.1758	0.4793	-0.0709	0.1593	0.0427	0.061	0.3132	0.0948	0.4057	1.7118	23.2207	-2.7906
12	5.7269	-5.5659	0.9797	5.5908	0.1415	6.8329	0.5829	1.301	-0.5381	-0.8109	-0.4721	0.2412	0.3346	0.1545	-0.0626	0.3311	0.0992	-0.0021	0.4554	-0.0175	0.3547	2.2184	10.788	-7.583
13	4.8897	-5.4303	4.6965	6.6243	-5.114	4.5355	-0.1588	0.1158	0.4716	-0.2795	0.31	-0.1311	-0.1462	-0.547	-0.0462	0.1852	0.0194	0.0231	0.3225	0.1293	0.2543	-1.9057	22.8133	-0.0986
14	9.9611	-5.902	-7.4928	5.1402	3.8353	5.9646	0.927	-3.5987	1.9891	1.7547	0.2149	-0.6899	-2.6565	0.8693	-1.1037	0.8516	0.0324	-0.0676	0.8152	-0.1984	0.5572	13.0145	19.7143	4.4596
15	7.5622	-2.5205	-0.9476	2.0781	2.0646	7.6113	0.1051	0.3713	0.2949	-0.3508	-0.1257	-0.0394	-0.4432	0.0945	0.0652	0.131	-0.0032	0.0465	0.1706	0.0603	0.2006	19.7818	9.5731	-5.2632
16	6.1912	0.461	-0.385	4.9327	-0.3821	6.5958	-0.1482	-0.32	0.0208	0.3441	0.1403	0.0828	-0.0433	0.0237	-0.0376	0.1408	0.0254	-0.0129	0.0566	0.0035	0.1201	-25.1545	2.7102	29.5113
17	2.1203	1.2133	1.3559	3.9316	5.7152	8.9913	-0.1004	-0.2334	-0.0711	0.3082	-0.1532	-0.2986	-0.2698	0.0764	0.2075	0.4211	-0.0817	-0.1086	0.3574	0.0975	0.1384	-12.8485	15.386	33.8387
18	4.449	-2.049	2.8814	3.1136	0.0064	6.2705	0.0166	-0.0851	-0.2107	0.4963	-0.1423	0.1195	-0.9273	-0.1092	0.061	0.5014	-0.1397	-0.159	0.4525	0.0442	0.0275	-11.588	20.842	34.1644
19	7.6171	0.7149	-0.0793	4.1684	0.9732	5.5379	-0.2628	-0.1578	-0.1	0.422	-0.0047	-0.5495	0.1693	0.4501	0.164	0.2278	-0.0026	-0.0527	0.1416	0.0438	0.1839	-21.2768	4.4077	30.3387
20	5.2904	0.8142	0.2247	7.5648	0.0797	5.7032	0.1169	-0.2178	0.0877	0.0967	-0.1361	0.0583	-0.0196	-0.2534	0.0353	0.0975	0.0228	-0.0181	0.2092	0.0389	0.088	-14.7508	19.4698	10.0018
21	5.513	-2.8128	-0.252	4.1003	0.0613	4.3368	0.2564	-0.515	0.7552	0.3162	0.0978	0.0672	-0.8927	-0.3827	-0.2352	0.4524	0.0937	0.0571	0.2822	-0.0242	0.3073	-23.1593	32.2132	21.0588
22	8.3837	-0.4243	-0.4741	6.8037	0.8384	7.8416	-0.0342	-0.5216	1.5665	-0.1754	0.3123	-0.8685	-1.1539	0.042	-0.2879	0.6902	0.0989	0.0684	0.3233	-0.0815	0.641	-23.7535	36.9857	21.8366
23	6.7238	-1.9952	-0.3602	8.1127	2.3589	6.4016	0.0254	-0.3072	1.1125	0.0763	-0.1048	0.3165	-0.966	-0.4116	0.0707	0.4859	0.136	0.1445	0.3748	-0.0254	0.479	-27.268	33.5277	22.0194
24	5.3182	-0.1913	-3.9528	2.8186	1.2759	7.0365	0.1615	-0.0734	0.1085	0.2199	-0.1248	-0.0468	-0.4037	0.0293	-0.0459	0.1673	0.0193	-0.0346	0.1329	0.0546	0.1529	-14.6863	24.2796	11.0787
25	8.6483	-2.614	-0.2861	6.7168	3.0716	7.3002	0.0161	-1.1192	-0.6222	1.2164	0.1673	-0.9452	0.519	1.0784	-0.0704	0.4317	-0.0552	-0.158	0.6338	0.1997	0.3452	-6.0968	17.3593	18.9932
26	7.3911	-0.4822	-0.2968	4.7581	1.6149	2.384	-0.2669	0.4823	0.0132	-0.6302	0.1971	0.23	0.2797	-0.0086	0.0705	0.309	-0.0345	-0.022	0.2292	0.019	0.1241	1.8877	50.3395	-41.3661
27	2.6463	-1.5901	-3.1198	5.1654	3.3655	4.362	-0.0011	0.2197	0.1596	-0.0788	-0.2589	-0.2308	0.0034	-0.0657	0.2004	0.4195	0.1626	0.0275	0.4588	0.0142	0.1563	13.9483	37.4611	-44.3999
28	7.4333	1.7153	-5.0654	4.8215	-0.6861	5.7106	0.1596	0.0268	0.2347	-0.2367	-0.1097	0.2377	0.7118	-0.0284	-0.0424	0.4699	0.1481	0.0076	0.4721	-0.0478	0.1623	15.5645	32.139	-44.8427
29	8.6351	-1.4453	-0.8484	7.2258	1.9008	7.204	-0.059	-0.0453	-0.0485	-0.4574	-0.01	-0.7511	-0.1813	0.635	-0.024	0.2767	0.015	0.0126	0.1712	0.0235	0.1492	6.2874	48.8662	-40.8618
30	4.5062	-4.1526	-2.6361	9.562	-1.2416	3.8917	0.2359	0.7783	-0.1926	-0.8603	-0.3503	-0.6582	0.3351	0.471	0.0603	0.2998	0.0154	0.0853	0.5191	-0.019	0.3081	15.7831	-0.3418	8.1293
31	6.6357	2.842	1.607	6.9687	0.4762	2.3709	-0.1131	-0.3183	-0.5054	0.2172	-0.063	0.4706	0.1916	-0.7649	0.1735	0.1605	-0.1212	0.0336	0.3505	-0.0502	0.3514	4.8818	0.4635	20.6412
32	7.2695	3.5576	0.7144	5.3027	0.5199	0.7633	-0.0279	-0.2892	0.1853	-0.3321	0.169	-0.3205	-0.2286	0.1735	-0.1341	0.0374	-0.2956	0.0079	0.6035	-0.0712	0.3466	-6.3118	-11.2505	11.9298
33	3.7177	-0.8281	-2.9371	6.8708	2.7671	4.0955	-0.146	-0.111	-0.1173	0.1286	0.3038	-0.6148	0.0585	0.2162	-0.1035	0.1844	-0.0974	-0.0093	0.2863	-0.0977	0.2984	-7.5052	-13.4477	9.6191
34	8.2945	2.4462	3.5099	4.4345	-0.3512	4.8715	-0.1522	0.0256	-0.5188	-0.2752	0.1086	0.6624	0.0776	-0.7472	0.0106	0.1752	-0.0619	0.0274	0.3356	-0.144	0.3632	2.7919	-1.0694	17.545
35	7.0251	1.0463	2.1058	9.1892	2.7762	2.0172	0.3285	-0.4275	-1.4514	0.4608	-0.3483	0.0859	0.45	-0.2597	0.0569	0.3456	-0.0991	0.0116	0.4169	0.0631	0.6045	-20.8927	63.7449	13.3135
36	7.8969	6.9327	3.8962	7.5139	1.5151	6.0018	0.3803	-0.2412	-1.4958	0.3221	0.133	-0.4358	0.2202	0.3476	-0.5081	0.2213	-0.0969	-0.047	0.4275	-0.0134	0.6661	-18.254	66.5789	12.5756
37	5.3798	6.068	2.8954	6.929	3.0931	1.9091	0.6181	-0.7337	2.8799	1.6884	-0.2395	2.4528	-0.8958	-1.041	-0.2815	0.5147	0.0053	0.1994	0.6868	-0.052	0.9601	-31.8968	72.4776	10.9153
38	1.2291	1.0887	0.8585	2.099	0.8156	1.1276	0.2365	-0.1074	-0.5268	0.2714	-0.0419	-0.2489	0.0706	-0.0422	0.1195	0.015	-0.3024	0.0083	0.3033	-0.2917	0.7517	-37.8487	59.105	5.2424
39	1.6071	-1.161	0.9027	1.5516	-0.4679	0.5501	0.1428	-0.3243	1.0611	0.4608	-0.285	-1.0398	-0.0706	-0.0155	0.038	0.3023	-0.2134	0.0368	0.3088	-0.1018	0.8485	-41.1953	50.643	9.4112
40	6.3398	1.7953	0.4476	5.2754	-0.7975	3.338	0.1051	0.4499	0.0357	-0.2968	-0.1027	-0.2195	-0.6359	0.4634	0.1008	0.3161	-0.1712	0.0295	0.383	-0.1534	0.4938	-38.127	65.2253	0.1418
41	7.7542	0.8722	2.4693	8.6696	-2.2621	3.7827	0.2273	-0.2471	-0.359	0.4597	-0.2708	-0.1259	-0.0038	0.0124	0.1075	0.1747	-0.1163	-0.0098	0.3566	-0.1952	0.7283	-45.1963	56.8648	3.4788
42	6.9732	5.3802	0.8597	8.5221	0.4144	0.1199	1.318	-1.2692	-1.131	2.6962	-0.8477	1.5359	1.7715	-1.1722	-0.3974	1.0417	-0.3877	-0.0264	0.8174	0.1065	1.2745	-33.2255	55.6983	13.9037
43	8.9482	3.8501	-2.2666	5.0878	-1.7799	3.605	0.1949	-0.4877	-0.9819	0.2095	0.1546	0.4798	0.1212	-0.2013	-0.1788	0.1399	-0.1644	-0.0367	0.3904	-0.0258	0.5198	-22.9344	66.6672	10.7087
44	5.0155	-0.345	0.2966	2.4721	0.6491	3.16	0.0936	0.028	0.0064	0.1098	0.2013	-0.6628	0.0886	0.2557	-0.1873	0.2161	0.0523	-0.0031	0.3128	-0.1942	0.472	-29.5394	66.3175	-20.323
45	6.4169	-3.8588	-2.0175	3.4938	-0.664	5.2499	-0.2219	-0.3434	0.1425	0.045	0.1159	0.6991	0.0206	-0.8284	0.0429	0.1736	0.0149	0.009	0.4207	-0.1725	0.6069	-19.2577	54.7518	-29.1318
46	8.1461	-1.1528	-0.3605	4.1541	1.5471	6.784	-0.0656	0.6961	-0.3438	-0.1798	-0.2844	0.9074	-0.0774	-0.688	0.3094	0.1983	-0.0085	-0.0029	0.378	-0.1244	0.4262	-16.7257	51.7425	-33.806
47	0.4452	-0.9014	-0.6975	2.0046	0.9911	2.0354	-0.0791	0.103	-0.629	0.8861	-0.3589	0.6133	-0.2519	-1.0045	0.4334	0.625	0.0749	0.0032	0.8691	-0.1793	0.6715	-14.7781	55.9473	-20.3136
48	7.1123	-3.0742	-0.1817	5.4785	-0.4339	4.1502	-0.1072	0.1664	-0.3597	0.3645	0.1438	0.0952	0.1948	0.0765	-0.0691	0.195	0.0738	0.0016	0.4164	-0.1819	0.4461	-28.7155	64.0812	-22.0521
49	7.3757	-4.512	-0.7473	8.5369	0.9752	5.2068	0.3645	0.8439	0.029	-0.5297	-0.6093	0.1854	0.0348	-0.0479	0.2958	0.2276	0.1311	-0.0332	0.3491	-0.1017	0.2861	-20.6604	-32.9389	97.3631
50	9.353	-4.4004	-0.961	6.8202	-0.1064	4.4069	0.5965	0.6767	0.3084	-0.7765	-0.1543	-0.2131	-0.2647	-0.6746	-0.19	0.2504	0.1193	0.084	0.4165	-0.0945	0.4338	-4.0771	-40.3624	89.8828
51	4.2823	-2.7206	0.1003	6.4681	1.2117	5.3554	0.7754	0.4008	1.3598	-0.5344	-0.1519	-0.7768	-0.7457	0.1154	-0.5522	0.3253	0.0578	0.2106	0.3971	-0.0217	0.7663	-0.3317	-37.5892	88.5212
52	3.5028	-3.7636	-1.692	7.8212	1.2612	1.1805	0.7491	1.0475	-3.0117	-1.1078	-1.0272	1.7033	0.8912	-0.4672	0.3896	0.4817	0.0749	-0.0449	0.5812	-0.266	0.9679	-11.4002	-45.131	90.7584
53	5.5088	-3.7228	-1.167	8.6833	1.0473	3.2947	0.3509	0.8852	-0.2941	-0.7213	-0.3767	0.1069	-0.1632	-0.3077	-0.0493	0.1642	0.0586	-0.0219	0.3629	-0.1327	0.3692	-19.0084	-32.1468	96.4839
54	3.1107	-0.5544	-1.6098	8.1133	1.3977	4.287	0.0449	-0.1408	-0.9527	0.5095	0.2557	-0.9675	0.1688	-0.4281	-0.1346	0.8283	-0.1332	-0.2414	0.666	-0.006	0.5282	3.6452	15.53	-31.8324
55	5.4837	1.6578	2.199	3.102	0.4011	4.4295	-0.0584	0.4882	-0.315	-0.6968	-0.0126	0.1672	0.1993	-0.2853	0.169	0.3423	0.0365	-0.1055	0.4259	-0.0526	0.2153	8.0084	26.0994	-28.0161
56	7.1134	0.6879	-1.4115	6.7716	-1.178	1.9928	0.22	0.4468	0.2856	-0.6626	-0.4901	0.3643	-0.3066	-0.3035	0.3297	0.3241	0.1234	-0.0651	0.3774	-0.0149	0.175	15.3009	34.9491	-24.045
57	1.9887	2.1136	0.1914	6.5797	-2.2124	6.9781	-0.0135	0.0518	-0.2033	0.1665	-0.0194	1.7631	0.3263	-1.5633	-0.0681	0.1964	-0.041	-0.0059	0.5621	-0.0916	0.568	5.0813	29.269	-17.6548
58	6.1242	-1.5609	-1.2522	1.9827	0.3901	8.2751	0.1989	0.187	0.1971	-0.1606	-0.5946	-0.3373	-0.4154	0.5869	0.4196	0.2651	0.0049	-0.0786	0.2986	0.074	0.2148	18.1082	34.8111	-20.5254
59	3.6028	2.0054	2.3266	3.6452	-0.382	2.6178	-0.0355	1.1303	-1.1626	-0.0616	0.6261	-0.97	1.1015	1.303	-0.8075	0.555	0.2767	-0.1872	0.7374	-0.4075	0.4876	16.0845	22.2026	-29.8258
60	3.3354	3.6421	2.0595	6.9409	0.6895	2.4829	-0.1045	0.7242	-0.7586	-0.0835	0.2567	0.1191	0.8597	-0.3656	-0.2847	0.4577	-0.0272	-0.0699	0.4827	-0.1722	0.394	1.9947	22.5889	-30.6516
61	3.4752	3.2159	-2.1012	7.8143	-0.6944	1.5927	0.402	-0.327	-1.3298	0.352	0.0931	0.5003	1.8038	-1.7116	-0.5012	1.0959	-0.5183	-0.1256	0.8421	0.1625	0.6053	-7.682	14.6093	-28.5677
62	5.4531	2.6847	3.0405	2.8389	1.344	8.5346	-0.0314	1.7337	-0.978	-0.6421	0.4448	-0.6815	1.0751	0.3648	-0.5402	0.7832	-0.032	-0.1154	0.6919	-0.1437	0.6102	5.1235	20.2315	-35.2217
63	7.4403	-0.053	0.37	5.1714	4.2593	9.0091	-0.6855	-0.8209	-0.1068	-0.3508	0.2312	-1.08	0.4533	-0.0577	0.308	0.9975	0.2315	0.2881	0.5095	0.0999	0.7157	-14.9285	-15.7141	42.7915
64	2.5079	-2.2986	-2.6581	3.9978	2.8009	3.2125	-0.7612	-0.5268	-0.5986	0.1013	0.3946	-0.6414	0.7414	0.2526	0.3869	1.2314	0.291	0.4453	0.5752	0.1603	0.9029	-16.7238	-18.8143	41.5089
65	2.7441	-0.4929	-2.8782	0.9587	1.0969	5.6279	-0.4683	-0.5588	-0.4636	0.3102	0.1543	-0.2074	1.1875	-0.0098	0.4092	0.8939	0.1665	0.1073	0.4083	0.0479	0.263	-27.7361	-4.594	53.3068
66	5.5359	-0.6212	0.3511	8.865	-1.7477	7.1073	-0.3536	0.0168	-0.2276	-0.1667	-0.2408	0.4532	1.4839	-0.6097	0.553	0.6914	0.0205	0.1105	0.3621	-0.0496	0.2854	-32.4607	-4.3705	45.5542
67	2.1967	-0.2786	-0.9437	7.2114	-0.564	3.2116	-0.6282	-0.0219	-0.9504	0.4454	-0.211	0.6426	1.8437	-0.505	0.499	0.9926	-0.0077	0.38	0.5458	-0.005	0.3161	-34.2433	-6.2183	51.0454
68	0.8962	-1.9569	-0.0873	4.2398	0.183	-0	-0.5047	-0.2175	-0.6204	-0.2692	0.1406	-0.4518	1.2896	0.5984	0.1682	0.9952	0.2883	0.2531	0.3834	0.0612	0.5275	-19.8821	-13.8248	43.6514
69	2.1259	-2.1021	-1.0905	6.1201	4.3435	5.0998	0.6707	0.456	0.0245	-0.6124	-0.1168	-1.0736	-0.1291	0.237	-0.7473	1.6503	-0.519	-0.5051	0.9905	0.2619	0.934	15.8117	67.2843	-54.8803
70	0.7062	-0.3852	0.8514	0.5678	0.3724	2.9826	-0.3467	0.4558	0.6882	0.5945	-0.2449	-0.7633	-2.0775	0.4505	0.618	2.3935	-0.5794	-0.4404	1.3287	0.118	0.8368	13.5328	68.7497	-60.0074
71	4.1691	4.1933	2.4756	4.2191	2.468	1.5437	0.2913	-0.8573	1.3549	0.7305	-0.5867	0.4757	-0.5489	0.4131	0.2954	1.3735	-0.5205	-0.3468	0.8203	0.1738	1.071	10.9029	71.6753	-49.4457
72	0.7158	1.0568	0.9776	2.801	0.3837	4.5034	-0.4137	0.1305	0.4671	-0.0384	-0.1159	0.1725	-1.2425	-0.0393	0.1867	1.1449	-0.3757	-0.2331	0.6233	0.0618	0.2285	-1.0438	57.3981	-60.3139
73	5.9298	3.0786	1.9513	8.8996	4.4177	6.3271	-0.6772	-0.2976	1.3592	-0.7424	-0.4555	0.9439	-2.069	-0.6462	0.9886	0.8759	0.0476	-0.2515	0.5266	-0.1388	0.3686	-6.1343	55.8639	-58.8457
74	5.4618	1.4062	3.4222	2.7584	0.7022	4.4864	-0.2703	-0.2066	0.2362	0.4812	0.3133	0.0564	-1.1363	-0.4682	0.2436	1.5319	-0.2817	-0.7551	0.8561	0.3807	0.9927	-3.6953	71.2024	-62.6548
75	1.9177	-0.7474	-2.2773	0.3543	0.7951	2.8381	-0.2159	-0.061	0.3775	-0.1355	0.1624	-0.2129	-0.7033	0.6197	0.0711	2.0142	-0.4046	-0.5867	0.6436	0.096	0.7528	-1.7135	64.9601	-64.165
76	4.3884	1.0481	-0.2506	3.5935	3.0428	8.2233	-0.2451	0.2692	0.3376	-0.3506	-0.43	0.2835	-1.2375	-0.1568	0.489	0.6999	-0.2167	-0.2065	0.5433	-0.0176	0.1385	-2.689	53.7813	-61.1146
77	3.5801	3.1102	-0.3449	5.1284	-0.6618	1.843	-0.3201	0.3633	0.5667	-0.0915	-0.0767	-0.0745	-0.5753	0.1928	-0.1253	1.2766	-0.6403	-0.216	0.809	0.2796	0.5311	7.7044	66.5709	-53.543
78	2.1142	1.4949	0.8334	3.3615	0.6837	0.888	0.0201	0.0537	-0.3899	0.2319	0.065	-0.3865	0.0966	0.0557	-0.0673	1.41	-0.7994	-0.4196	0.8326	0.2099	0.7691	12.4128	70.4225	-53.9478
79	2.4803	0.3726	1.1578	4.3458	0.6153	0.8663	-0.24	0.093	0.1474	-0.9462	0.2228	-0.7208	0.76	0.0102	-0.0062	1.5516	-0.5443	-0.1786	0.8539	0.1723	0.985	-34.2763	-28.1144	57.6808
80	7.6472	6.1855	3.4297	6.0839	3.2778	1.7729	0.6673	-1.486	0.8373	0.7114	-0.9724	0.5495	0.0563	-0.5409	0.4505	1.5644	-0.785	-0.2662	0.9783	-0.0067	0.9659	-36.7541	-28.5471	68.9181
81	7.0991	5.1181	4.5634	9.5196	5.8427	4.1688	-0.775	1.0354	-0.418	-1.0685	1.1442	-0.2292	0.4433	0.0277	-0.5115	1.2818	-0.3389	-0.421	0.8488	-0.0036	1.0309	-18.4431	-17.325	70.7199
82	7.1912	0.8857	-0.0591	7.8845	-0.5417	5.4685	0.3711	-0.1229	-0.9855	0.2806	0.3495	0.4614	0.9495	-0.8938	-0.82	0.8291	-0.3894	-0.2179	0.8981	0.093	0.7206	-25.6915	-16.5967	75.4256
83	4.4683	-2.1621	2.6632	7.7419	0.4901	4.7883	0.3476	-0.1248	-0.2948	-0.1669	-0.2858	0.1259	0.3756	-0.3384	0.2587	1.2612	-0.5078	-0.0748	0.8012	-0.0036	0.5857	-22.2652	-14.1292	78.2095
84	8.382	6.2535	6.7284	5.0801	5.4021	5.7532	-0.0088	0.2597	-0.9014	0.0723	-0.4416	0.3954	2.1188	-0.757	0.383	1.659	-0.3107	-0.151	0.6723	-0.0185	1.1943	-20.0961	-29.7039	74.3047
85	3.5698	-2.6688	-4.2716	4.3897	0.1756	8.9142	0.4793	0.1304	-1.0166	0.5034	-0.5071	0.358	2.1178	-0.8962	0.059	1.4999	-0.2193	-0.3777	0.8271	0.12	0.9722	-19.3536	-24.0356	73.7878
86	1.0112	0.2016	0.1533	2.5225	-2.5913	7.1916	0.3431	-0.3048	-0.0494	0.6477	-0.157	-0.0232	0.0095	-0.0585	-0.1421	1.0092	-0.4789	-0.1987	0.8477	0.0648	0.6301	-17.9866	-12.9576	78.5661
87	1.9899	1.9593	0.2536	2.3492	0.6033	0.317	-0.0028	0.1563	-0.4662	-0.3868	0.3361	-0.1762	0.1458	-0.0531	-0.0042	1.338	-0.6781	-0.3867	0.7051	0.1774	1.1771	-31.2231	-24.9555	64.2407
88	9.0319	-1.5161	0.9839	4.948	-1.3503	4.1924	0.5292	0.7302	0.7439	0.4546	-0.4747	0.8046	-0.4423	0.2788	-0.0319	1.0978	0.3504	0.2543	0.6082	0.0956	0.6	5.9124	71.2722	-89.2886
89	9.3021	-3.0274	-0.2805	2.785	-0.8049	7.0393	0.3261	-0.0462	1.523	-0.3353	0.1317	-0.3065	-0.8976	0.0516	-0.2466	0.9012	0.4314	0.2687	0.7488	0.0703	0.8879	9.602	69.6357	-89.6123
90	6.5124	-5.4887	1.8076	4.8852	-2.7452	6.2368	0.1888	-0.4279	-1.3498	0.0114	0.3555	1.4491	1.2433	-0.0991	-0.6154	1.2554	0.3092	0.2826	0.7176	0.2499	0.9666	1.2179	64.7295	-78.4038
91	6.6657	-2.6194	-1.7019	7.2599	1.1459	3.9198	0.3805	-0.0233	0.0664	0.5154	0.193	-0.382	-0.7304	-0.0423	-0.3891	1.0625	0.3751	0.3624	0.5165	0.1253	0.976	-3.6003	80.2785	-76.1156
92	3.2757	2.2448	-1.4376	6.1601	2.4643	6.9928	0.3904	0.0697	0.1707	0.8441	0.3495	-0.1819	-0.963	0.0236	-0.6151	1.1064	0.1889	0.4358	0.4443	0.1266	1.0994	-6.0613	81.2561	-69.5178
93	6.5311	-5.8168	-4.2233	9.2754	7.1749	5.5858	0.33	0.6528	-0.4716	-0.2656	-0.397	1.427	-0.3861	-0.5292	0.1395	1.0232	0.5016	0.3993	0.6801	0.2852	1.0104	-3.5714	76.3436	-81.5213
94	6.6683	-4.1229	-4.6403	8.6976	2.1796	3.3066	0.3463	0	0.2569	-0.4145	0.3616	-0.0717	-0.5805	-0.1078	-0.5949	0.6956	0.356	0.0842	0.5885	0.0229	0.7278	7.7589	70.7875	-85.8748

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 5 through 14 )	A	5 - 14
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 15 through 19 )	A	15 - 19
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 20 through 32 )	A	20 - 32
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 33 through 38 )	A	33 - 38
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 39 through 59 )	A	39 - 59
6	X-RAY DIFFRACTION	6	chain 'B' and (resid 5 through 32 )	B	5 - 32
7	X-RAY DIFFRACTION	7	chain 'B' and (resid 33 through 59 )	B	33 - 59
8	X-RAY DIFFRACTION	8	chain 'C' and (resid 4 through 32 )	C	4 - 32
9	X-RAY DIFFRACTION	9	chain 'C' and (resid 33 through 38 )	C	33 - 38
10	X-RAY DIFFRACTION	10	chain 'C' and (resid 39 through 59 )	C	39 - 59
11	X-RAY DIFFRACTION	11	chain 'D' and (resid 5 through 14 )	D	5 - 14
12	X-RAY DIFFRACTION	12	chain 'D' and (resid 15 through 24 )	D	15 - 24
13	X-RAY DIFFRACTION	13	chain 'D' and (resid 25 through 32 )	D	25 - 32
14	X-RAY DIFFRACTION	14	chain 'D' and (resid 33 through 38 )	D	33 - 38
15	X-RAY DIFFRACTION	15	chain 'D' and (resid 39 through 59 )	D	39 - 59
16	X-RAY DIFFRACTION	16	chain 'E' and (resid 1 through 33 )	E	1 - 33
17	X-RAY DIFFRACTION	17	chain 'E' and (resid 34 through 59 )	E	34 - 59
18	X-RAY DIFFRACTION	18	chain 'F' and (resid 5 through 32 )	F	5 - 32
19	X-RAY DIFFRACTION	19	chain 'F' and (resid 33 through 61 )	F	33 - 61
20	X-RAY DIFFRACTION	20	chain 'G' and (resid 5 through 32 )	G	5 - 32
21	X-RAY DIFFRACTION	21	chain 'G' and (resid 33 through 60 )	G	33 - 60
22	X-RAY DIFFRACTION	22	chain 'H' and (resid 5 through 19 )	H	5 - 19
23	X-RAY DIFFRACTION	23	chain 'H' and (resid 20 through 38 )	H	20 - 38
24	X-RAY DIFFRACTION	24	chain 'H' and (resid 39 through 56 )	H	39 - 56
25	X-RAY DIFFRACTION	25	chain 'H' and (resid 57 through 62 )	H	57 - 62
26	X-RAY DIFFRACTION	26	chain 'I' and (resid 3 through 33 )	I	3 - 33
27	X-RAY DIFFRACTION	27	chain 'I' and (resid 34 through 58 )	I	34 - 58
28	X-RAY DIFFRACTION	28	chain 'J' and (resid 5 through 32 )	J	5 - 32
29	X-RAY DIFFRACTION	29	chain 'J' and (resid 33 through 62 )	J	33 - 62
30	X-RAY DIFFRACTION	30	chain 'K' and (resid 1 through 6 )	K	1 - 6
31	X-RAY DIFFRACTION	31	chain 'K' and (resid 7 through 38 )	K	7 - 38
32	X-RAY DIFFRACTION	32	chain 'K' and (resid 39 through 58 )	K	39 - 58
33	X-RAY DIFFRACTION	33	chain 'L' and (resid 1 through 32 )	L	1 - 32
34	X-RAY DIFFRACTION	34	chain 'L' and (resid 33 through 59 )	L	33 - 59
35	X-RAY DIFFRACTION	35	chain 'M' and (resid 5 through 19 )	M	5 - 19
36	X-RAY DIFFRACTION	36	chain 'M' and (resid 20 through 32 )	M	20 - 32
37	X-RAY DIFFRACTION	37	chain 'M' and (resid 33 through 38 )	M	33 - 38
38	X-RAY DIFFRACTION	38	chain 'M' and (resid 39 through 59 )	M	39 - 59
39	X-RAY DIFFRACTION	39	chain 'N' and (resid 3 through 9 )	N	3 - 9
40	X-RAY DIFFRACTION	40	chain 'N' and (resid 10 through 24 )	N	10 - 24
41	X-RAY DIFFRACTION	41	chain 'N' and (resid 25 through 32 )	N	25 - 32
42	X-RAY DIFFRACTION	42	chain 'N' and (resid 33 through 38 )	N	33 - 38
43	X-RAY DIFFRACTION	43	chain 'N' and (resid 39 through 58 )	N	39 - 58
44	X-RAY DIFFRACTION	44	chain 'O' and (resid 1 through 32 )	O	1 - 32
45	X-RAY DIFFRACTION	45	chain 'O' and (resid 33 through 58 )	O	33 - 58
46	X-RAY DIFFRACTION	46	chain 'P' and (resid 5 through 31 )	P	5 - 31
47	X-RAY DIFFRACTION	47	chain 'P' and (resid 32 through 38 )	P	32 - 38
48	X-RAY DIFFRACTION	48	chain 'P' and (resid 39 through 59 )	P	39 - 59
49	X-RAY DIFFRACTION	49	chain 'Q' and (resid 5 through 38 )	Q	5 - 38
50	X-RAY DIFFRACTION	50	chain 'Q' and (resid 39 through 60 )	Q	39 - 60
51	X-RAY DIFFRACTION	51	chain 'R' and (resid 5 through 31 )	R	5 - 31
52	X-RAY DIFFRACTION	52	chain 'R' and (resid 32 through 38 )	R	32 - 38
53	X-RAY DIFFRACTION	53	chain 'R' and (resid 39 through 59 )	R	39 - 59
54	X-RAY DIFFRACTION	54	chain 'S' and (resid 5 through 24 )	S	5 - 24
55	X-RAY DIFFRACTION	55	chain 'S' and (resid 25 through 60 )	S	25 - 60
56	X-RAY DIFFRACTION	56	chain 'T' and (resid 5 through 14 )	T	5 - 14
57	X-RAY DIFFRACTION	57	chain 'T' and (resid 15 through 24 )	T	15 - 24
58	X-RAY DIFFRACTION	58	chain 'T' and (resid 25 through 32 )	T	25 - 32
59	X-RAY DIFFRACTION	59	chain 'T' and (resid 33 through 38 )	T	33 - 38
60	X-RAY DIFFRACTION	60	chain 'T' and (resid 39 through 43 )	T	39 - 43
61	X-RAY DIFFRACTION	61	chain 'T' and (resid 44 through 48 )	T	44 - 48
62	X-RAY DIFFRACTION	62	chain 'T' and (resid 49 through 59 )	T	49 - 59
63	X-RAY DIFFRACTION	63	chain 'U' and (resid 5 through 19 )	U	5 - 19
64	X-RAY DIFFRACTION	64	chain 'U' and (resid 20 through 38 )	U	20 - 38
65	X-RAY DIFFRACTION	65	chain 'U' and (resid 39 through 48 )	U	39 - 48
66	X-RAY DIFFRACTION	66	chain 'U' and (resid 49 through 60 )	U	49 - 60
67	X-RAY DIFFRACTION	67	chain 'V' and (resid 5 through 31 )	V	5 - 31
68	X-RAY DIFFRACTION	68	chain 'V' and (resid 32 through 57 )	V	32 - 57
69	X-RAY DIFFRACTION	69	chain 'W' and (resid 6 through 19 )	W	6 - 19
70	X-RAY DIFFRACTION	70	chain 'W' and (resid 20 through 24 )	W	20 - 24
71	X-RAY DIFFRACTION	71	chain 'W' and (resid 25 through 38 )	W	25 - 38
72	X-RAY DIFFRACTION	72	chain 'W' and (resid 39 through 60 )	W	39 - 60
73	X-RAY DIFFRACTION	73	chain 'X' and (resid 5 through 14 )	X	5 - 14
74	X-RAY DIFFRACTION	74	chain 'X' and (resid 15 through 19 )	X	15 - 19
75	X-RAY DIFFRACTION	75	chain 'X' and (resid 20 through 24 )	X	20 - 24
76	X-RAY DIFFRACTION	76	chain 'X' and (resid 25 through 36 )	X	25 - 36
77	X-RAY DIFFRACTION	77	chain 'X' and (resid 37 through 43 )	X	37 - 43
78	X-RAY DIFFRACTION	78	chain 'X' and (resid 44 through 58 )	X	44 - 58
79	X-RAY DIFFRACTION	79	chain 'Y' and (resid 5 through 28 )	Y	5 - 28
80	X-RAY DIFFRACTION	80	chain 'Y' and (resid 29 through 38 )	Y	29 - 38
81	X-RAY DIFFRACTION	81	chain 'Y' and (resid 39 through 48 )	Y	39 - 48
82	X-RAY DIFFRACTION	82	chain 'Y' and (resid 49 through 59 )	Y	49 - 59
83	X-RAY DIFFRACTION	83	chain 'Z' and (resid 5 through 14 )	Z	5 - 14
84	X-RAY DIFFRACTION	84	chain 'Z' and (resid 15 through 19 )	Z	15 - 19
85	X-RAY DIFFRACTION	85	chain 'Z' and (resid 20 through 24 )	Z	20 - 24
86	X-RAY DIFFRACTION	86	chain 'Z' and (resid 25 through 32 )	Z	25 - 32
87	X-RAY DIFFRACTION	87	chain 'Z' and (resid 33 through 58 )	Z	33 - 58
88	X-RAY DIFFRACTION	88	chain '1' and (resid 5 through 19 )	1	5 - 19
89	X-RAY DIFFRACTION	89	chain '1' and (resid 20 through 31 )	1	20 - 31
90	X-RAY DIFFRACTION	90	chain '1' and (resid 32 through 38 )	1	32 - 38
91	X-RAY DIFFRACTION	91	chain '1' and (resid 39 through 58 )	1	39 - 58
92	X-RAY DIFFRACTION	92	chain '2' and (resid 5 through 28 )	2	5 - 28
93	X-RAY DIFFRACTION	93	chain '2' and (resid 29 through 43 )	2	29 - 43
94	X-RAY DIFFRACTION	94	chain '2' and (resid 44 through 57 )	2	44 - 57